FastQCFastQC Report
Wed 27 Apr 2016
SRR616840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57455511
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1904840.3315330360563672No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1197220.20837339694011248No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1025640.17851029120600806No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC917370.1596661458637101TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG137000.032.4034339
CTCGTAT145300.031.77486442
TCTCGTA150950.029.98933641
TCGTATG156300.029.89845743
ACACGTC176200.028.97360413
CGTATGC163650.028.8031344
CGTCTGA179150.028.25810416
CACCGAT176700.028.12789331
ACCGATG179850.027.6974132
CCGATGT171550.027.63485133
ACGTCTG185200.027.37121815
ATCTCGT168900.026.81551640
TCACCGA185550.026.7499530
GTCACCG186150.026.74831229
CACGTCT191750.026.54184514
CACACGT201600.025.45701812
GCACACG203150.025.3401811
ATAGAAT541500.024.6530882
CGCGATT487700.024.46943320
TATAGAA569800.023.5870931
Produced by FastQC (version 0.11.5)