FastQCFastQC Report
Wed 27 Apr 2016
SRR616839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616839_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54058089
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1415830.26190899940987555No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1318950.24398753718430558No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT718160.13284968323612031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT543150.026.2033732
TATAGAA560850.025.5940691
CGCGATT504500.025.58913420
GCGATTT525100.024.57705321
CGATTTT533500.024.24491122
GAATTCG617500.023.1576615
TAGAATT638200.022.473
AATTCGC642450.022.1952846
AGAATTC668400.021.4446324
ATTCGCG713800.020.1942627
TTCGCGG781950.018.604018
TCGCGGC783350.018.5851389
TCGCGAT801200.017.65742919
CTCGCGA813850.017.47143618
CGCGGCC836650.017.45761510
GCTCGCG838650.017.24957717
CTCGTAT56450.017.1798242
CCGCTCG852100.017.05388515
CGCTCGC859450.016.88446616
AATCTCG63350.016.58709339
Produced by FastQC (version 0.11.5)