FastQCFastQC Report
Wed 27 Apr 2016
SRR616838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616838_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53649803
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1676230.31243917149145917No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1136740.211881486312261No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1035900.19308551794682266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT92050.025.42241933
CTCGTAT85750.025.40089242
TATCTCG87550.025.26372539
CGCGATT481750.023.77111420
TCGTATG95150.023.08012843
ATAGAAT551450.022.9899062
GCGATTT500900.022.80432921
CGTATGC97550.022.60438744
CGATTTT506700.022.56549822
GTCACGC103150.022.4463129
TCTCGTA97750.022.00641341
TATAGAA587500.021.7729971
ACGCCAA112100.020.93588432
AGTCACG111500.020.926528
ACACGTC116650.020.44433413
ACGTCTG121150.019.68491415
CGTCTGA121300.019.67908316
TAGAATT648750.019.6597733
GAATTCG646350.019.659645
ATCTCGT113650.019.34338440
Produced by FastQC (version 0.11.5)