FastQCFastQC Report
Wed 27 Apr 2016
SRR616837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51933582
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1256410.24192631272766818No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1001920.1929233381206018No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC690280.1329159232652198TruSeq Adapter, Index 12 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT638450.12293586835585499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT119250.029.00157742
AATCTCG123800.028.40778539
TCTCGTA123000.027.76977741
ACACGTC140650.027.57714713
TCGTATG130450.027.5632843
CGTATGC133850.026.91332644
ACGTCTG150200.025.8085615
CGTCTGA152200.025.26247416
ATCTCGT139550.024.7504740
CACGTCT159100.024.49210714
CGCGATT424200.024.41847620
ATAGAAT474900.024.3269442
CACACGT165400.023.43697512
TATAGAA498750.023.3626141
GCACACG171000.023.19572611
GCGATTT448350.023.12861821
CGATTTT456400.022.76506422
GTATGCC166850.021.7655545
GTAATCT162900.021.54809237
GAATTCG540200.021.4112225
Produced by FastQC (version 0.11.5)