FastQCFastQC Report
Wed 27 Apr 2016
SRR616836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51489680
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1641030.3187104678063643No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1304620.2533750452517864No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT924140.17948062602059286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG82450.026.90849739
CTCGTAT87850.025.69019942
CGCGATT502550.024.81084420
TCTCGTA90550.024.45205541
GCGATTT524150.023.7758921
CGATTTT527450.023.69112222
CACCGAT110850.022.22762331
ACACGTC115350.021.747413
ATAGAAT652750.021.4176352
ATCTCGT104600.021.36114940
TCGTATG108500.021.29804443
ACCGATG115500.021.29380232
CGTCTGA116500.021.26223616
CGTATGC112450.020.82990644
ACGTCTG119900.020.77190815
CCGATGT113500.020.75653533
GTCACCG119400.020.59767529
TATAGAA688450.020.4674931
TCACCGA121900.020.15692530
CACGTCT126050.019.90123414
Produced by FastQC (version 0.11.5)