FastQCFastQC Report
Wed 27 Apr 2016
SRR616835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616835_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73608054
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1756290.238600248826032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1677260.22786365198569167No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT768860.10445324366271115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT710600.026.472852
TATAGAA751750.025.1048871
GAATTCG871900.021.500575
TAGAATT877700.021.4712943
CGCGATT771850.021.1248220
AGAATTC903650.020.8522034
GCGATTT798150.020.40618121
CGATTTT816150.019.99747822
AATTCGC968800.019.2827196
ATTCGCG1100100.017.1326187
TTCGCGG1242100.015.351498
TCGCGGC1250250.015.208289
CGCGGCC1316750.014.49320410
TCGCGAT1286450.014.38152919
CTCGCGA1294100.014.2878118
GATTTTT1172200.014.15945823
GCTCGCG1339600.014.07506517
CCGCTCG1350300.014.0169215
CGCTCGC1358800.013.89938416
GGCCGCT1404500.013.53848213
Produced by FastQC (version 0.11.5)