FastQCFastQC Report
Wed 27 Apr 2016
SRR616834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68738393
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1707900.2484637660935716No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1471570.21408268884028173No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT912280.13271767933242198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT638150.025.0747622
TATAGAA670000.024.113941
GAATTCG784650.020.2927575
TAGAATT796000.020.1447353
CGCGATT681850.020.03930320
AGAATTC815450.019.6394164
GCGATTT706900.019.30053521
CGATTTT721900.018.918222
TATCTCG65150.018.40755539
AATTCGC870100.018.2067766
CGCCAAT70450.017.82071733
GTCACGC74600.016.73877329
TCGTATG75450.016.4310543
ATTCGCG984500.016.1962557
CTCGTAT76100.016.0244542
TCTCGTA78500.014.81847841
ACACGTC89050.014.47774513
TTCGCGG1123850.014.3121488
CGTATGC86850.014.30028544
TCGCGGC1135050.014.14121159
Produced by FastQC (version 0.11.5)