FastQCFastQC Report
Wed 27 Apr 2016
SRR616833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63797335
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1508160.23639858937681332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1441180.22589971822490704No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT777210.12182483798108495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT617400.025.9685822
TATAGAA640450.025.1557141
TCGTATG91150.022.98074543
AATCTCG95600.021.77050839
GAATTCG745150.021.4590595
CTCGTAT95450.021.42678542
ACACGTC107450.021.2748213
TAGAATT757000.021.2599073
TCTCGTA95100.021.12783441
AGAATTC771250.020.8641344
CGTATGC102550.020.57979444
CGCGATT667700.020.5111820
GCGATTT688850.019.8454921
CGATTTT700200.019.58485422
AATTCGC825150.019.2994846
ACGTCTG121250.018.90910715
CGTCTGA125050.018.29848716
CACGTCT125650.018.15741714
ATCTCGT119450.017.31067840
ATTCGCG951100.016.8454597
Produced by FastQC (version 0.11.5)