FastQCFastQC Report
Wed 27 Apr 2016
SRR616832_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616832_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56801491
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT11877712.0910912356156284No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT6121171.0776424865326157No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1999700.35205061782621166No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT976890.1719831614983487No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTC745400.13122894960627002No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTG570520.10044102539491437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA4218350.038.2147941
TGTATAG653450.036.4479641
GCGTTTT2151000.036.02716445
ATAGAAT4484950.035.7476963
TATAGAA4522750.035.529912
GAATTCG4525650.035.017396
TAGAATT4579150.034.9934964
AGAATTC4578800.034.7857485
AATTCGC4547600.034.739797
ATTCGCG4601500.034.399868
TCGCGGC4694050.033.8342710
TTCGCGG4681800.033.832089
CGCGATA2379950.033.81608621
TCGCGAT4767700.033.54368620
CGCGGCC4756750.033.49511
CTCGCGA4774200.033.48212419
TAATACG2223600.033.4407426
CCGCTCG4827950.033.2939416
GCGGCCG4795700.033.29046612
GCTCGCG4818500.033.2591318
Produced by FastQC (version 0.11.5)