FastQCFastQC Report
Wed 27 Apr 2016
SRR616832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56801491
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT777560.1368907728143967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA736470.12965680777640148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT407000.026.1856062
TATAGAA452400.023.7818031
CGCGATT396050.023.75093820
GAATTCG451450.023.7367025
ACACGTC90750.023.20502913
AATTCGC477200.022.2531246
GCGATTT427100.022.09273521
TAGAATT489500.022.0756533
CGATTTT429300.021.89566422
AGAATTC496450.021.6714554
CTCGTAT92550.021.05651542
AATCTCG91950.021.04757539
ATTCGCG527700.020.4347727
TCTCGTA97800.019.71942741
CACGTCT112150.018.9777514
ACGTCTG114400.018.87979715
TCGTATG108050.018.514743
TTCGCGG593000.018.2490638
TCGCGGC597050.018.159279
CGTCTGA119800.018.14138816
Produced by FastQC (version 0.11.5)