FastQCFastQC Report
Wed 27 Apr 2016
SRR616831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85342728
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1351380.1583474106897544No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1141150.13371379457192883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT610750.025.653882
TATAGAA662300.023.7069631
CGCGATT602450.023.348220
GAATTCG684250.023.0559125
GCGATTT644100.021.8908121
TAGAATT729800.021.6879253
AATTCGC722950.021.6132056
CGATTTT650200.021.58508922
AGAATTC756500.020.916574
ATTCGCG791400.020.0508617
TATCTCG87150.019.13360839
ACACGTC96750.017.85935213
TTCGCGG900100.017.7718878
CGCCAAT97550.017.76059733
TCGCGGC908000.017.5924859
CTCGCGA932100.016.87212818
TCGCGAT928000.016.85940219
GTCACGC106550.016.19608729
CCGCTCG997100.016.10169415
GCTCGCG995450.016.0696217
Produced by FastQC (version 0.11.5)