FastQCFastQC Report
Wed 27 Apr 2016
SRR616830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51541016
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1257260.24393387976674735No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT936730.18174457406893182No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT801580.15552273940428338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT387500.026.40777620
CGATTTT445850.023.0563622
GCGATTT448350.022.96721
ATAGAAT584150.020.1143972
TATAGAA600600.019.6040761
GAATTCG663700.017.6496945
TAGAATT668900.017.5987363
AGAATTC690550.016.988894
AATTCGC697100.016.7756846
ATTCGCG753000.015.553667
GATTTTT682150.015.42058323
TCGCGAT742850.015.39523319
CTCGCGA766750.014.88167218
TCGCGGC813200.014.4400689
GCTCGCG803700.014.41642417
TTCGCGG817700.014.4062758
CCGCTCG812950.014.28100715
CGCTCGC818850.014.15917916
TCGTATG59550.014.05732143
GCCGCTC853700.013.6266114
Produced by FastQC (version 0.11.5)