FastQCFastQC Report
Wed 27 Apr 2016
SRR616829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55707065
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1937210.34774942819191784No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1371590.24621473057322266No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190140.2136425604185035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG99350.030.19067439
CTCGTAT104000.028.92425242
CGCCAAT113400.028.1177133
CGCGATT574400.027.99446120
ACACGTC118150.027.16871813
GTCACGC118150.026.6147829
ACGTCTG124900.026.28279115
TCTCGTA113700.026.05138641
CACGTCT127300.025.88885714
CGTCTGA127500.025.68596316
AGTCACG128550.024.52991328
CGATTTT649950.024.41396722
GCGATTT650250.024.3958621
ACGCCAA134450.023.92779732
CACACGT138300.023.32148412
ATAGAAT760800.023.2426972
TCGTATG133700.023.09044343
TCACGCC141750.022.3233430
ATCTCGT134650.022.19393540
TATAGAA805500.022.0550671
Produced by FastQC (version 0.11.5)