FastQCFastQC Report
Wed 27 Apr 2016
SRR616828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52498080
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1298100.2472661857347926No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT805820.15349513734597534No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT767280.14615391648608864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT357550.026.42161420
CTCGTAT102350.026.01664242
ACACGTC114300.025.56277313
CGTCTGA116150.024.80836716
ACGTCTG119500.024.48120715
AATCTCG108250.024.4169839
TCGTATG113900.023.97618743
TCTCGTA110850.023.50703641
CACGTCT125750.023.40856214
CGTATGC118500.023.2123544
CGATTTT408350.023.02050222
GCGATTT409400.022.8594821
CACACGT135900.021.74229412
ATCTCGT124850.021.09981740
GCACACG146700.020.3511911
ATAGAAT535950.019.7170312
GTATGCC145300.018.9605945
TATAGAA563200.018.8685191
GAATTCG574700.018.3561425
TAGAATT607250.017.4598523
Produced by FastQC (version 0.11.5)