FastQCFastQC Report
Wed 27 Apr 2016
SRR616827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47767077
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1299070.27195928275033454No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT801200.16773058983701264No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT754940.15804609522161048No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC729740.15277049504201398TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG124300.033.0711939
CACGTCT146250.031.40494514
CTCGTAT136850.031.11323742
ACACGTC146550.031.09011713
ACGTCTG148600.030.92575815
CGTCTGA149200.030.87080616
TCTCGTA138250.030.22656841
TCGTATG143200.030.22411243
GTCACCG155750.029.07421329
CGTATGC150950.028.93434144
CACCGAT155650.028.89560531
CCGATGT152050.028.3226933
ACCGATG159700.028.10768932
TCACCGA161000.028.03073130
CACACGT164550.027.75591512
ATCTCGT153300.027.34511240
CGCGATT332500.026.21756220
GCACACG180550.025.68551611
GTATGCC182800.024.15648845
GCGATTT386050.022.68273421
Produced by FastQC (version 0.11.5)