FastQCFastQC Report
Wed 27 Apr 2016
SRR616826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51882153
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1488060.28681539102666No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1036920.1998606341567976No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT997440.19225108102202312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT421650.022.934620
GCGATTT446050.021.69515621
CGATTTT452600.021.38122722
ATAGAAT528250.020.7848632
TATAGAA558100.019.8139671
GAATTCG600250.018.3704325
TAGAATT611900.018.0133233
AATTCGC631800.017.4423896
AGAATTC636850.017.3288064
ACACGTC73550.016.54944813
ATTCGCG677650.016.414967
AATCTCG70500.015.51027239
TCTCGTA67650.015.46526741
TCGTATG73050.015.30749943
CTCGTAT69300.015.12941242
TTCGCGG750150.015.0084578
TCGCGGC749600.014.9864519
GATTTTT670550.014.74371823
ACGTCTG85550.014.62256915
TCGCGAT749200.014.40619419
Produced by FastQC (version 0.11.5)