FastQCFastQC Report
Wed 27 Apr 2016
SRR616825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616825_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53394102
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1376600.25781873810706657No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT982820.1840690194583664No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT972470.18213060311417917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT490300.023.5959822
CGCGATT444150.023.34815820
GCGATTT468600.022.11072321
CGATTTT475400.021.84657922
TATCTCG57650.021.8165939
TATAGAA533850.021.8151511
CGCCAAT62750.021.0483233
GAATTCG552850.020.9385175
TAGAATT573950.020.239323
AATTCGC589200.019.6467426
AGAATTC591650.019.5729894
GTCACGC69000.019.04390529
ACACGTC72350.019.00077613
CTCGTAT67600.018.63845842
ATTCGCG639400.018.3224187
TCTCGTA72400.017.3719141
TTCGCGG713150.016.61068
TCGCGGC713250.016.5830369
AGTCACG80000.016.45349528
ACGTCTG86150.016.08770415
Produced by FastQC (version 0.11.5)