FastQCFastQC Report
Wed 27 Apr 2016
SRR616824_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616824_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48055734
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT9097041.8930186354036336No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT5333771.1099133352119852No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1398350.2909850466543701No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA788040.1639845933890012No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT731690.1522586253702836No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT675350.14053473826869445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA3695550.036.779841
GCGTTTT1706600.035.3487845
ATAGAAT3977800.034.1327363
TATAGAA3981500.034.054212
CGCGATA1875650.033.8666221
GAATTCG3990750.033.4179536
TAATACG1746100.033.4142426
TAGAATT4066000.033.385574
GCGATAA1832200.033.2345622
AGAATTC4065500.033.1716585
AATTCGC4017150.033.1614077
ATACGAC1777450.033.07966228
AATACGA1713600.033.05762527
CGATAAT1827250.033.0280523
TCGCGAT4025350.032.92307320
TACGACT1820050.032.8584329
ATTCGCG4062550.032.8122678
CGCGATT1915850.032.69778421
CGACTCA1815100.032.69198231
ATAATAC1787950.032.68237725
Produced by FastQC (version 0.11.5)