FastQCFastQC Report
Wed 27 Apr 2016
SRR616824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616824_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48055734
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1307440.27206742903978953No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT998710.2078232745336904No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT839310.17465345550647504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT393900.024.32162920
GCGATTT417000.022.95813221
CGATTTT420250.022.80742322
ATAGAAT483350.022.119812
TATAGAA511150.021.0705241
GAATTCG532350.020.0288565
ACACGTC72250.020.02353113
AATCTCG67800.019.57929839
TAGAATT555400.019.3556143
CTCGTAT68400.019.14421842
AATTCGC560200.018.992976
TCTCGTA69100.018.82021541
AGAATTC573750.018.709124
ATTCGCG604500.017.8430237
TCGTATG76850.017.8296143
CGTATGC79000.017.40133544
ACGTCTG87650.016.53109215
CACGTCT89350.016.44319514
TTCGCGG664950.016.3664258
TCGCGGC668250.016.24529
Produced by FastQC (version 0.11.5)