FastQCFastQC Report
Wed 27 Apr 2016
SRR616823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58998771
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1851960.31389806408001275No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1367310.23175228514505836No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1186560.20111605375644181No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC871960.14779290910992027TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG133800.033.0599839
ACACGTC160900.030.9870913
CTCGTAT150200.030.76790242
TCTCGTA151400.030.0936141
TCGTATG164850.028.45660243
CACCGAT170300.028.27456731
ACCGATG172300.028.03773332
CCGATGT164250.027.95968833
ACGTCTG178800.027.74649415
GTCACCG174950.027.65159829
ATCTCGT167250.027.3358640
CACGTCT183100.027.23005114
TCACCGA176000.027.17987630
CGTATGC175300.027.02977244
CACACGT184900.026.94060912
CGTCTGA186600.026.7192816
GCACACG200450.025.04149211
CGCGATT501150.023.8801720
GTATGCC204400.023.13757145
GCGATTT525850.022.69002221
Produced by FastQC (version 0.11.5)