FastQCFastQC Report
Wed 27 Apr 2016
SRR616822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55598469
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1887110.3394176195751002No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1208940.21744123925426795No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1106950.19909720895372138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT494350.024.06752220
GCGATTT517250.023.0237421
CGATTTT528850.022.51022122
ATAGAAT646150.020.7045612
TATAGAA670600.020.070021
GAATTCG725250.018.4339925
TAGAATT731750.018.3532643
AATCTCG72450.017.6704839
TCGTATG75000.017.54967543
AATTCGC763900.017.5042576
AGAATTC766400.017.4148654
ACACGTC84950.017.34813513
CTCGTAT76250.016.7013342
TCTCGTA74350.016.49263441
ATTCGCG818450.016.4365587
CGTATGC82000.016.13384844
GATTTTT774850.015.69758623
TTCGCGG892150.015.2073648
TCGCGGC894300.015.0726839
TCGCGAT894150.014.75564119
Produced by FastQC (version 0.11.5)