FastQCFastQC Report
Wed 27 Apr 2016
SRR616821_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616821_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62812947
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT8340791.3278775154427955No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT4950010.7880556853987444No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1066890.16985192559107282No Hit
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTGTAGGGCGTTTT985680.15692306237438597No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA718540.11439361378793453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA3810700.037.7946241
ATAGAAT4043050.035.5356033
TATAGAA4061950.035.377382
GCGTTTT1797800.035.34693545
GAATTCG4083750.034.7379346
TAGAATT4150500.034.625154
AATTCGC4107700.034.5884747
CGCGATA1936000.034.4969221
ATTCGCG4146600.034.2718358
AGAATTC4157700.034.2489135
TAATACG1804500.034.0362226
CGCGATT2027450.033.88134821
TCGCGAT4137900.033.82543620
TCGCGGC4201500.033.7846410
GCGATAA1897900.033.76255422
TTCGCGG4217750.033.7047429
CGATAAT1899650.033.47832523
AATACGA1780350.033.47653227
ATACGAC1845850.033.4446928
CTCGCGA4193600.033.4254219
Produced by FastQC (version 0.11.5)