FastQCFastQC Report
Wed 27 Apr 2016
SRR616821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62812947
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1438110.22895120650842893No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1136560.18094358795170046No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT673700.10725495812192987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG81800.024.53505539
CGCCAAT91800.023.11233533
GTCACGC100350.021.16552529
ACACGTC101750.021.00702913
CTCGTAT99650.020.11755642
TCTCGTA99150.019.92400441
TCGTATG104900.019.64693543
CGCGATT354800.019.31615820
AGTCACG112950.018.88411128
ACGTCTG119000.018.01861615
CGTATGC113450.017.98776644
ATAGAAT455150.017.7910582
GCGATTT389050.017.71397621
ACGCCAA121950.017.65649832
CGATTTT391550.017.58363522
CACACGT124050.017.5752912
CACGTCT123200.017.31303214
ATCTCGT116650.016.97356640
CGTCTGA128750.016.82885416
TATAGAA493550.016.543181
Produced by FastQC (version 0.11.5)