FastQCFastQC Report
Wed 27 Apr 2016
SRR616820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41248610
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1545920.3747811138363208No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1273000.30861646004556276No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1053210.25533224028639995No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC640730.1553337191241111TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC118400.029.87285813
AATCTCG111500.029.1586839
CTCGTAT112750.028.33649442
TCTCGTA112900.028.2191341
ACGTCTG127950.027.6783615
TCGTATG120050.027.60672643
CACGTCT129850.027.2906914
CACACGT131600.027.08165412
CGTCTGA132950.026.55274616
CGTATGC126550.026.3665544
ATCTCGT127050.025.05856140
CGCGATT406900.024.55655320
GCACACG150450.024.12223411
GCGATTT428550.023.36847721
CGATTTT432600.023.12889922
GTATGCC146200.023.02282145
ATAGAAT499650.022.7044662
GTAATCT145000.022.42201837
TATAGAA524200.021.7662281
TAATCTC161400.020.21339638
Produced by FastQC (version 0.11.5)