FastQCFastQC Report
Wed 27 Apr 2016
SRR616819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51038758
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1269180.24866984419957866No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1032720.20234034691831645No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT725360.1421194457749148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG84350.028.19445439
CTCGTAT93300.026.5509142
ACACGTC101050.026.27359213
CGCGATT424150.024.6611820
TCGTATG102700.024.40554443
TCTCGTA101250.023.97729541
CGTATGC105800.023.83931244
CACCGAT109650.023.72045331
ATAGAAT496750.023.706622
GCGATTT449000.023.36645521
CCGATGT107200.023.31809233
ACCGATG113250.023.22469732
CGATTTT458400.022.87750622
TATAGAA520400.022.7881411
GTCACCG115750.022.70364629
CGTCTGA118700.022.65118416
TCACCGA118800.022.0450130
ACGTCTG121300.021.96163415
CACGTCT123750.021.58138314
GAATTCG555200.021.0771145
Produced by FastQC (version 0.11.5)