FastQCFastQC Report
Wed 27 Apr 2016
SRR616818_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616818_2.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences170945296
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA210800.010.23031218-19
CTCGAAT551050.010.1173491
CTCGTTT566350.09.4445861
CTCGTAT292100.09.156011
CTCAAAT1191900.09.139981
CTGGAAT1675000.09.1209921
CTCGGAT647250.08.7844931
CTCCGAT406950.08.7585321
CGCCACT405150.08.7229951
CGACGAT294450.08.7073261
CTCGGTT366500.08.6690051
CGGGGAT500400.08.5896411
CTCAGAT1344750.08.50111
CTCGATT240350.08.4710461
CGTATAG194050.08.2978218-19
CGGGAAT410700.08.2379011
CGCCAAC364000.08.2037281
CTTTATT2041200.08.1496211
CTGGGAT2101150.08.1419991
CGCCAAT245550.08.0255031
Produced by FastQC (version 0.11.5)