FastQCFastQC Report
Tue 26 Apr 2016
SRR616818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616818_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences170945296
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA226350.010.54393318-19
CTCGTTT599350.09.3202121
CTCGAAT588300.09.1834141
CTGGAAT1808400.09.1814371
CCGGGAT441450.09.1541261
CGGGAAT408600.09.1220381
CGGGGAT493700.08.9285431
CTCGGTT373750.08.3966321
CGTATAG214050.08.31249118-19
CTCAAAT1247550.08.2857471
CGACAAT241950.08.0218291
CGCGAAT106050.07.96710161
CTCGGAT677300.07.93390561
CTCGCGT86400.07.87913131
CGGCAAT267450.07.87076331
CGGAAAT396450.07.8427871
CTCGTAT342400.07.82586151
CTCAGAT1453800.07.73791931
CTCGATT255500.07.72868061
CTGGGAT2271100.07.71903371
Produced by FastQC (version 0.11.5)