FastQCFastQC Report
Tue 26 Apr 2016
SRR616817_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616817_2.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences166713119
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2098540.12587731622968437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT299800.010.6571371
ACGTATA211500.09.87956818-19
CTCAAAT1223550.09.7805751
CTCGTTT585500.09.7038461
CTCGAAT536150.09.6925211
CTGGAAT1668650.09.61922551
CGACGAT305950.09.3084921
CTCCGAT430100.09.2082791
CTTTATT2249900.09.13534451
CTCGGAT638150.09.1267521
CTCAGAT1399750.09.079281
CGGGGAT550800.08.977231
CTCGGTT375000.08.8877061
CGCGCTA84600.08.8702979
CTGGGAT2136450.08.4365321
CGACAAT214550.08.3765691
CGGGAAT410050.08.3251211
CTCGATT242700.08.2865461
CTTGGAT1209000.08.2780631
CGTATAG192350.08.19674718-19
Produced by FastQC (version 0.11.5)