FastQCFastQC Report
Tue 26 Apr 2016
SRR616817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616817_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences166713119
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1886360.11315006349320354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTT581650.010.0912771
CTCGAAT540300.09.6330761
CTCAAAT1221800.09.5109361
ACGTATA207950.09.50087618-19
CTGGAAT1673300.09.3087081
CTTTATT2251150.09.1827681
GTCGACC208450.09.1810531
CTCGGTT357700.09.0675751
CGATTAG132350.09.0058024
CTCGATT240750.08.9947261
CGACAAT213500.08.8526771
CTCGTAT328550.08.8313911
CTCAGAT1383200.08.8062651
CTCCGAT431750.08.67828751
CTCGGAT653550.08.6759071
CGACGAT305950.08.5369281
CGGGGAT531050.08.4773931
CTTGGAT1207300.08.2523881
CCGGGAT457950.08.181791
CGGGAAT397950.08.150451
Produced by FastQC (version 0.11.5)