FastQCFastQC Report
Tue 26 Apr 2016
SRR616816_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616816_2.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences156601624
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA192100.010.16138618-19
CTCGTTT545400.09.9742571
CGATTAG130300.09.66954
CTCGAAT502950.09.6437311
CGACGAT285600.09.2906421
CTCGTAT285150.09.2886271
GTCGACC211250.09.2515571
CTCAAAT1101750.09.0680261
CTGGAAT1564100.09.0294571
CTCCGAT409450.08.9889671
CTTTATT1993550.08.8947741
CTCAGAT1269600.08.8055291
CGCGCTA87100.08.7781929
CTCGGTT349400.08.6693441
CTCGGAT619650.08.6256051
CGACAAT207550.08.6145941
CGGGAAT396600.08.3330091
CTGGGAT2004000.08.3121261
CGGGGAT520350.08.1606661
CTTGGAT1117650.08.0327811
Produced by FastQC (version 0.11.5)