FastQCFastQC Report
Tue 26 Apr 2016
SRR616816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616816_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences156601624
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACC199500.010.542911
ACGTATA193050.010.35299618-19
CTCGTTT514550.09.993251
CTCGAAT489150.09.6941851
CTCGGTT329100.09.1676941
CTCGTAT302850.09.087491
CTGGAAT1508000.09.0462731
CTCAAAT1066000.09.00315951
CGCGCTA75450.08.9521989
CGACGAT278850.08.8419481
CTTTATT1948800.08.8146231
CTCCGAT396950.08.70399951
CGGGGAT482550.08.6726621
CGTATAG176250.08.662374518-19
CTCGGAT589200.08.3875911
CGACCCG246350.08.1865393
CTTGGAT1093400.08.1841341
GGTCGAC191100.08.1826235
CTCAGAT1234150.08.1401341
CTCGCAT309900.08.1305171
Produced by FastQC (version 0.11.5)