FastQCFastQC Report
Sat 18 Jun 2016
SRR799686_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR799686_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8000000
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT647220.809025No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA498460.623075No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA372470.46558750000000004No Hit
AAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAA128230.1602875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2540700.072.777246
TGGTATC2532200.072.5836947
AGTGGTA2551250.072.365075
CAGTGGT2572000.071.96324
GTATCAA2557100.071.929539
GGTATCA2567050.071.7470868
AAGCAGT2699350.069.948221
AGCAGTG2749250.068.6662452
GCAGTGG2770550.067.979843
GAGTACT137850.050.55988720-21
GTCACCG86350.049.923822-23
GTACTTT125700.048.256722-23
TCACCGG90250.048.06105422-23
AGAGTAC231350.047.0405918-19
AGTACTT133300.046.62576320-21
CAGAGTA590150.046.4820818-19
CAGAGTG576800.044.80986418-19
AGAGTGG247700.043.77458218-19
GAGTCAC124350.043.21939520-21
AGAGTCA226500.042.66356318-19
Produced by FastQC (version 0.11.5)