##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR799686_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8000000 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.467603 31.0 26.0 31.0 16.0 34.0 2 27.709168375 31.0 26.0 31.0 16.0 34.0 3 27.6407345 31.0 26.0 31.0 16.0 34.0 4 30.362796 35.0 28.0 37.0 12.0 37.0 5 30.60328 35.0 28.0 37.0 16.0 37.0 6 30.481861375 35.0 28.0 37.0 13.0 37.0 7 30.4074405 35.0 28.0 37.0 13.0 37.0 8 30.364519375 35.0 28.0 37.0 13.0 37.0 9 31.351370625 35.0 28.0 39.0 13.0 39.0 10-11 31.3915286875 35.0 28.0 39.0 14.5 39.0 12-13 31.0903658125 35.0 27.0 39.0 12.0 39.0 14-15 32.4217173125 36.5 28.5 40.0 16.0 41.0 16-17 31.812537875 36.0 27.0 40.0 12.0 41.0 18-19 31.8264745625 36.0 27.0 40.0 13.0 41.0 20-21 31.808595562500003 36.0 27.0 40.0 13.0 41.0 22-23 26.5844136875 30.5 14.5 38.5 7.5 40.5 24-25 27.0533765625 28.5 17.5 37.5 10.0 40.0 26-27 28.696554562499998 32.5 21.5 38.0 9.0 40.0 28-29 29.331078312499997 33.5 23.0 38.5 9.0 40.0 30-31 28.95288275 32.5 22.0 38.5 8.5 40.0 32-33 28.455957125 32.0 21.0 38.0 8.0 40.0 34-35 28.734800874999998 32.5 21.5 38.0 8.0 40.0 36-37 28.8175938125 33.0 22.0 38.0 8.0 40.0 38-39 28.654723437500003 33.0 22.0 38.0 8.0 40.0 40-41 28.5108945625 32.0 22.0 38.0 8.0 40.0 42-43 28.5289040625 32.5 22.0 38.0 8.0 40.0 44-45 28.289185125 32.0 21.0 38.0 8.0 40.0 46-47 28.067556125 31.5 20.5 38.0 8.0 40.0 48-49 27.822591625 31.0 20.0 38.0 8.0 40.0 50-51 27.493534750000002 31.0 20.0 37.0 8.0 40.0 52-53 27.4323016875 31.0 20.0 37.0 8.0 40.0 54-55 27.343380625 31.0 20.0 37.0 8.0 40.0 56-57 27.123527875 30.0 20.0 36.5 8.0 40.0 58-59 26.962964 30.0 19.0 36.0 8.0 39.0 60-61 26.719059625 30.0 18.5 36.0 7.5 39.0 62-63 26.50470975 30.0 18.0 35.0 7.0 39.0 64-65 26.281335125 29.0 18.0 35.0 7.0 39.0 66-67 26.0404330625 29.0 18.0 35.0 7.0 38.5 68-69 25.827486375 29.0 18.0 35.0 7.0 38.0 70-71 25.38974675 29.0 17.0 34.0 7.0 37.5 72-73 25.273319125 29.0 17.5 34.0 7.0 37.0 74-75 25.1070931875 29.0 17.0 34.0 7.0 36.5 76-77 24.8322633125 28.0 17.0 34.0 7.0 36.0 78-79 24.664452812500002 28.0 17.0 34.0 7.0 36.0 80-81 24.4650616875 28.0 15.5 34.0 7.0 35.0 82-83 24.308924 27.5 16.0 34.0 7.0 35.0 84-85 24.098690062499998 27.0 15.0 34.0 7.0 35.0 86-87 23.982287125 27.0 15.0 33.5 7.0 35.0 88-89 23.8085255625 27.0 15.0 33.0 7.0 35.0 90-91 23.7701844375 27.0 15.0 33.0 7.0 35.0 92-93 23.632363875000003 27.0 15.0 33.0 7.0 35.0 94-95 23.5710045625 27.0 15.0 33.0 7.0 35.0 96-97 23.419666874999997 26.0 14.5 33.0 7.0 35.0 98-99 23.268199062500003 26.0 14.5 33.0 7.0 35.0 100-101 22.208164125000003 24.5 10.5 32.0 7.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4051847524480898 1101 2 0.37127302647338567 1101 3 0.4563319928777574 1101 4 0.46659299105494867 1101 5 0.38044415800411 1101 6 0.3922002294419755 1101 7 0.40194719108765753 1101 8 0.5217061706324237 1101 9 0.6130528227786627 1101 10-11 0.5896336071835364 1101 12-13 0.5572540809985398 1101 14-15 0.5725705478329246 1101 16-17 0.5998970348671939 1101 18-19 0.703700868367207 1101 20-21 0.7494751523538845 1101 22-23 5.8632153633663435 1101 24-25 4.613913383110724 1101 26-27 1.6853566454462339 1101 28-29 1.1025307761115712 1101 30-31 0.9073193453498796 1101 32-33 0.9267586738588989 1101 34-35 0.8026469448891334 1101 36-37 0.7685156367644801 1101 38-39 0.796066338363655 1101 40-41 0.8293886355853637 1101 42-43 0.7433575967888153 1101 44-45 0.6984250905049301 1101 46-47 0.5580491345106786 1101 48-49 0.5964845200216473 1101 50-51 0.7751813168653747 1101 52-53 0.680103963580617 1101 54-55 0.5597494186151906 1101 56-57 0.5220836168900256 1101 58-59 0.5155656376567102 1101 60-61 0.42364067990267884 1101 62-63 0.3952597698840741 1101 64-65 0.40918612824644995 1101 66-67 0.4271529621002266 1101 68-69 0.3903348880575379 1101 70-71 0.4004656075810935 1101 72-73 0.2864515973101298 1101 74-75 0.40381428255690466 1101 76-77 0.41792168623894455 1101 78-79 0.3491743533291327 1101 80-81 0.3352294738836257 1101 82-83 0.2800643009936401 1101 84-85 0.25976781774689073 1101 86-87 0.22541554234251393 1101 88-89 0.28092242314191296 1101 90-91 0.296001507866567 1101 92-93 0.2199294709069406 1101 94-95 0.16052819773295468 1101 96-97 0.15865268264384014 1101 98-99 0.1700992972531168 1101 100-101 0.1950108617534987 1102 1 0.2716163791320234 1102 2 0.18545810811894725 1102 3 0.3039515245941651 1102 4 0.1977518769016946 1102 5 0.21374849286054243 1102 6 0.11675048263004939 1102 7 0.29611729407756826 1102 8 0.26462289979724574 1102 9 0.26651234006314795 1102 10-11 0.13752813310177814 1102 12-13 0.30860835127695907 1102 14-15 0.3115141234776786 1102 16-17 0.38015046178745493 1102 18-19 0.40348464675877693 1102 20-21 0.39468003815072805 1102 22-23 4.69393010790337 1102 24-25 3.8461621961084553 1102 26-27 1.3482702951015604 1102 28-29 0.7543866370045222 1102 30-31 0.6195969708321698 1102 32-33 0.6449756172217604 1102 34-35 0.5401355072444964 1102 36-37 0.5001807144907389 1102 38-39 0.4268727783019841 1102 40-41 0.5646090345788899 1102 42-43 0.4874945103073216 1102 44-45 0.46650264951031417 1102 46-47 0.35974499471658916 1102 48-49 0.357411844193976 1102 50-51 0.5114227330863024 1102 52-53 0.36957301248063956 1102 54-55 0.24115070285120055 1102 56-57 0.26817601851677964 1102 58-59 0.20528875580243167 1102 60-61 0.1394067226694773 1102 62-63 0.09407305908392871 1102 64-65 0.1125198466548234 1102 66-67 0.0818334164346588 1102 68-69 0.14183453380732303 1102 70-71 0.13719667478526176 1102 72-73 0.0015702412170703894 1102 74-75 0.06170652446953895 1102 76-77 0.04615079308898018 1102 78-79 -0.0013282050226557374 1102 80-81 -0.0032781687466822973 1102 82-83 0.004192448239859203 1102 84-85 0.02054564319249863 1102 86-87 -0.010513566844252864 1102 88-89 0.008416702188451808 1102 90-91 -0.03381605143831479 1102 92-93 -0.07769548766507128 1102 94-95 -0.08649585258591941 1102 96-97 -0.06048144307456127 1102 98-99 -0.02965884908591221 1102 100-101 -0.03357867301629014 1103 1 -0.06814175427576075 1103 2 -0.15441310927817398 1103 3 -0.07533867065791355 1103 4 -0.12409048248218113 1103 5 -0.21269194434231764 1103 6 -0.1073226932932485 1103 7 -0.07085717230702215 1103 8 -0.07680634919995555 1103 9 0.15543187647861245 1103 10-11 -0.1524822574815481 1103 12-13 -0.05282410826804096 1103 14-15 -0.040187102881219516 1103 16-17 -0.013074718395774454 1103 18-19 -0.013710155778795752 1103 20-21 -0.02668329982990869 1103 22-23 -9.199617212079282 1103 24-25 -7.774402258187251 1103 26-27 -1.7776921991005068 1103 28-29 -0.3882849335740559 1103 30-31 -0.09311924129092475 1103 32-33 0.06801844314445304 1103 34-35 0.05211141195997726 1103 36-37 0.050363611778085016 1103 38-39 0.04148034665700351 1103 40-41 0.11781425873358486 1103 42-43 0.03055088134728834 1103 44-45 -0.024111358644329783 1103 46-47 -0.0725102022739712 1103 48-49 -0.08325945042006921 1103 50-51 0.0786262745351145 1103 52-53 -0.06018832509034766 1103 54-55 -0.09724804337516346 1103 56-57 -0.0801991455140616 1103 58-59 -0.19474683411481308 1103 60-61 -0.1496928625981937 1103 62-63 -0.18395823427842473 1103 64-65 -0.22340774807121377 1103 66-67 -0.24495142109978474 1103 68-69 -0.21904426704658775 1103 70-71 -0.2121693961856259 1103 72-73 -0.3104189588150774 1103 74-75 -0.32246892174448405 1103 76-77 -0.2643479488881404 1103 78-79 -0.291275378372557 1103 80-81 -0.2633868851544179 1103 82-83 -0.2918232030604564 1103 84-85 -0.2703922327386401 1103 86-87 -0.25830839361383084 1103 88-89 -0.22526413900908793 1103 90-91 -0.2785190115979681 1103 92-93 -0.26156387703191086 1103 94-95 -0.3223422030772767 1103 96-97 -0.31075575859473403 1103 98-99 -0.2867647353256899 1103 100-101 -0.27737876724932775 1104 1 -1.3476359508022533 1104 2 -1.195727711151477 1104 3 -1.0351899267929205 1104 4 -1.248609026411252 1104 5 -1.3325634153034684 1104 6 -0.48607825449732545 1104 7 -0.5338511707291254 1104 8 -0.26290489020026797 1104 9 -0.5004078986397467 1104 10-11 -0.5838653662573599 1104 12-13 -0.7742896614612107 1104 14-15 -1.321415049528131 1104 16-17 -1.633853005960031 1104 18-19 -1.4187259748554908 1104 20-21 -1.3497035215676867 1104 22-23 -9.982695863987548 1104 24-25 -8.226145281523717 1104 26-27 -3.3178529579373794 1104 28-29 -1.8772194078580036 1104 30-31 -1.655936620711831 1104 32-33 -1.8340414197297648 1104 34-35 -1.6670136688267974 1104 36-37 -1.830670788547355 1104 38-39 -1.7923307775933601 1104 40-41 -1.7149620543273478 1104 42-43 -1.791956827584471 1104 44-45 -1.6472737129546928 1104 46-47 -1.6051584328631243 1104 48-49 -1.7630988454472565 1104 50-51 -1.943596714483661 1104 52-53 -1.8448056567396627 1104 54-55 -1.7940768753149712 1104 56-57 -1.7555629911638704 1104 58-59 -1.7731146652964718 1104 60-61 -1.7849164230764174 1104 62-63 -1.7312928171489013 1104 64-65 -1.6594635517737295 1104 66-67 -1.6494316453033768 1104 68-69 -1.7922570935169198 1104 70-71 -1.7731545100080162 1104 72-73 -1.8665607450587594 1104 74-75 -1.8057855228355386 1104 76-77 -1.7079747923566764 1104 78-79 -1.7012013058896294 1104 80-81 -1.856747625106344 1104 82-83 -1.8411614184377 1104 84-85 -1.856906604311174 1104 86-87 -1.7894717319578248 1104 88-89 -1.8601734106758876 1104 90-91 -1.7559027906733355 1104 92-93 -1.7267207670142497 1104 94-95 -1.8018319135541176 1104 96-97 -1.891760396841672 1104 98-99 -1.923049071707446 1104 100-101 -1.8277816127097566 1105 1 0.49552714760093863 1105 2 0.4919591601477258 1105 3 0.5013558324543759 1105 4 0.512711933462846 1105 5 0.4876191270469832 1105 6 0.6010353044533545 1105 7 0.24805003585569807 1105 8 0.5944849318005438 1105 9 0.3963778478695481 1105 10-11 0.3234385600943632 1105 12-13 0.45326133151549897 1105 14-15 0.7857145723735357 1105 16-17 0.537226301280672 1105 18-19 0.4887718712373825 1105 20-21 0.7770475096494813 1105 22-23 3.3034637342373685 1105 24-25 3.2951308059455364 1105 26-27 1.187491107973024 1105 28-29 0.6406320696531225 1105 30-31 0.41931601374863803 1105 32-33 0.13839399881401704 1105 34-35 0.38124996785066756 1105 36-37 0.43338347195015103 1105 38-39 0.460267083077575 1105 40-41 0.5370911864045489 1105 42-43 0.5433964012959862 1105 44-45 0.4935301785506283 1105 46-47 0.3390974564565532 1105 48-49 0.3376104287228223 1105 50-51 0.48498569223241716 1105 52-53 0.4842720624833028 1105 54-55 0.45799719450143783 1105 56-57 0.6047233436250252 1105 58-59 0.5870248764991608 1105 60-61 0.47976859262713134 1105 62-63 0.49878747341136886 1105 64-65 0.4153779185229105 1105 66-67 0.38127183354048455 1105 68-69 0.43425208727012077 1105 70-71 0.4411776214098815 1105 72-73 0.3860811923517815 1105 74-75 0.43712588862198487 1105 76-77 0.5391431588089368 1105 78-79 0.4981382292084078 1105 80-81 0.49699996633800225 1105 82-83 0.4546394998648573 1105 84-85 0.4403978680565217 1105 86-87 0.3822436136669225 1105 88-89 0.38668398630778 1105 90-91 0.3278555830279508 1105 92-93 0.39168484206704335 1105 94-95 0.3579787476045979 1105 96-97 0.36685218165733247 1105 98-99 0.3328831693110814 1105 100-101 0.2236743600917741 1106 1 -0.233629296115204 1106 2 -0.28272062408342435 1106 3 -0.6291596206360559 1106 4 -0.360557205385426 1106 5 -0.38456757386561335 1106 6 -0.2710518502276784 1106 7 -0.19084677927298443 1106 8 -0.2311507496318299 1106 9 -0.2619571688377107 1106 10-11 -0.26820510373523376 1106 12-13 -0.7283698447459059 1106 14-15 -0.8524806359263053 1106 16-17 -0.4350069438885491 1106 18-19 -1.4414634297530533 1106 20-21 -1.301370080234971 1106 22-23 3.528967975286889 1106 24-25 3.121506268392203 1106 26-27 0.5946627491299274 1106 28-29 -0.8451017311729174 1106 30-31 -0.2731713096573003 1106 32-33 -0.07368002144166397 1106 34-35 -0.13180682875396244 1106 36-37 -0.09963446156305977 1106 38-39 -0.5241358154961873 1106 40-41 -1.252228139014722 1106 42-43 -0.5858665809729686 1106 44-45 -0.7923285782718388 1106 46-47 -0.5216011105777731 1106 48-49 -0.27189519168292264 1106 50-51 -1.5531586503336072 1106 52-53 -1.0246846498509719 1106 54-55 -0.5009097192893037 1106 56-57 -0.8873550248069684 1106 58-59 -0.8789658685191348 1106 60-61 -0.5806205189955875 1106 62-63 -0.38776065313508923 1106 64-65 -0.24877907451016057 1106 66-67 -0.2112944889242705 1106 68-69 -0.23830808481099908 1106 70-71 -0.9372334482722202 1106 72-73 -0.6404179185777821 1106 74-75 -0.5546497500383687 1106 76-77 -0.710094719727433 1106 78-79 -0.27592681311020684 1106 80-81 -0.47689005165842246 1106 82-83 -0.29740554246138373 1106 84-85 -0.3005585294201829 1106 86-87 -0.31021978656727 1106 88-89 -0.8757728958637117 1106 90-91 -0.4329118208027367 1106 92-93 -0.3206199444808959 1106 94-95 -0.28278917302961304 1106 96-97 -0.15209674193994616 1106 98-99 -0.22923043543989863 1106 100-101 -0.17894653147256534 1107 1 0.2802522130787466 1107 2 0.2538323350564369 1107 3 0.3255289176577669 1107 4 0.3129159507794199 1107 5 0.32595438330581317 1107 6 0.09239266424507875 1107 7 -0.11605406392567374 1107 8 -0.16915829441295216 1107 9 -0.01567902313021463 1107 10-11 0.31323258290935385 1107 12-13 -0.26241548582980556 1107 14-15 0.3184929895693216 1107 16-17 0.40468597021046904 1107 18-19 0.405779908300822 1107 20-21 0.04131321486775974 1107 22-23 4.016099306723071 1107 24-25 3.595405068641508 1107 26-27 0.9789745976332505 1107 28-29 0.2644277652837843 1107 30-31 0.3867404711746296 1107 32-33 0.14594971933970413 1107 34-35 0.21928545568240665 1107 36-37 -0.04929549429355973 1107 38-39 0.15360627552136918 1107 40-41 0.35725345566740785 1107 42-43 0.3698478404790997 1107 44-45 0.3776534332535597 1107 46-47 0.30786647319875726 1107 48-49 0.26046448789447396 1107 50-51 0.3963702508457274 1107 52-53 0.3241178510509961 1107 54-55 0.28643326419057047 1107 56-57 0.24780508595189943 1107 58-59 0.2622615734882956 1107 60-61 0.332335816914501 1107 62-63 0.18814111591761318 1107 64-65 0.19370481652176252 1107 66-67 0.20516508090012664 1107 68-69 0.25537300389921214 1107 70-71 0.3415608697760497 1107 72-73 0.2649886457434967 1107 74-75 0.12993724516317684 1107 76-77 0.23054187408206772 1107 78-79 0.25385038678900074 1107 80-81 0.24340055297317775 1107 82-83 0.25745747153196774 1107 84-85 0.29221836982614136 1107 86-87 0.2918372498018229 1107 88-89 0.32268622777425904 1107 90-91 0.2565438429413227 1107 92-93 0.22716816023074315 1107 94-95 0.2163825132698456 1107 96-97 0.24605809154502722 1107 98-99 0.3153247667953849 1107 100-101 0.22940812775132358 1108 1 0.6912105740607863 1108 2 0.7149604919527164 1108 3 0.7466604113458892 1108 4 0.81840038882061 1108 5 0.8809440781491382 1108 6 0.7926699475530938 1108 7 0.8433939859825621 1108 8 0.8824027946291153 1108 9 0.7833052423647864 1108 10-11 0.6162382574890799 1108 12-13 0.7953050968096917 1108 14-15 0.9718206300265777 1108 16-17 0.6973368174211778 1108 18-19 0.9131394897404306 1108 20-21 0.9476109212285202 1108 22-23 5.711395103013054 1108 24-25 4.785409814703158 1108 26-27 1.7070315995644414 1108 28-29 1.3807293835754209 1108 30-31 0.6563100841086573 1108 32-33 0.8596939348811006 1108 34-35 0.8071928596057667 1108 36-37 0.9644150843488966 1108 38-39 1.1014795692654893 1108 40-41 1.2149549574871195 1108 42-43 1.0218179454239689 1108 44-45 1.1101439022725437 1108 46-47 0.9478169051277234 1108 48-49 1.109144489834538 1108 50-51 1.2814470134970364 1108 52-53 1.1743431142875203 1108 54-55 1.194286826679246 1108 56-57 1.2442564635500837 1108 58-59 1.191282875742285 1108 60-61 1.1848996044247073 1108 62-63 1.1750780284868902 1108 64-65 1.1533110941661207 1108 66-67 1.1248987571710067 1108 68-69 1.1276301992132893 1108 70-71 1.2135293806809777 1108 72-73 1.159619971230331 1108 74-75 1.1597148859308497 1108 76-77 1.102133138572988 1108 78-79 1.1130762202642295 1108 80-81 1.175406231297572 1108 82-83 1.1227748217843754 1108 84-85 1.1081570825537206 1108 86-87 1.1570520423276278 1108 88-89 1.2898714002724745 1108 90-91 1.1793392902285156 1108 92-93 1.22532035295772 1108 94-95 1.186739530064166 1108 96-97 0.9885168575476797 1108 98-99 1.099888143278271 1108 100-101 1.0292214537012576 1110 1 0.33151509146781777 1110 2 0.3338000222046311 1110 3 0.1809660595091387 1110 4 0.38886761022754257 1110 5 0.33060797725214996 1110 6 0.302347651522755 1110 7 0.25914780478532506 1110 8 0.06873635412126688 1110 9 -0.29280727026553066 1110 10-11 0.10080631315519639 1110 12-13 0.27707170727195063 1110 14-15 0.16058064392835547 1110 16-17 0.4202036038140058 1110 18-19 0.4922950046248715 1110 20-21 0.32949008566965077 1110 22-23 -8.984223728124853 1110 24-25 -7.488692991748202 1110 26-27 -2.3260416683738434 1110 28-29 -1.1219185413512491 1110 30-31 -0.7761849216861485 1110 32-33 -1.0312577104759129 1110 34-35 -0.8250042822386341 1110 36-37 -0.8202063801692567 1110 38-39 -0.7664065725532296 1110 40-41 -0.9203810270871422 1110 42-43 -1.0369612149774277 1110 44-45 -0.905390624110634 1110 46-47 -0.8146585802836661 1110 48-49 -0.9732805497389663 1110 50-51 -0.9671497694978832 1110 52-53 -0.8658224089756921 1110 54-55 -0.9515910532885883 1110 56-57 -0.8236357283067157 1110 58-59 -0.6990198204019862 1110 60-61 -0.7607737135470103 1110 62-63 -0.5374368068483761 1110 64-65 -0.6311539295937152 1110 66-67 -0.7156470298444297 1110 68-69 -0.955339012377106 1110 70-71 -0.4299489633143345 1110 72-73 -0.19159104497525092 1110 74-75 -0.530688395723228 1110 76-77 -0.6809282990836785 1110 78-79 -0.8037916929964233 1110 80-81 -0.4481197152241094 1110 82-83 -0.637376007905349 1110 84-85 -0.7197569822830054 1110 86-87 -0.6920648629826864 1110 88-89 -0.5739657657476691 1110 90-91 -0.5728019766861259 1110 92-93 -0.609942597155225 1110 94-95 -0.6130128167850017 1110 96-97 -0.5394552427287671 1110 98-99 -0.5935925654572927 1110 100-101 -0.5186037094046512 1111 1 -0.23186838726705616 1111 2 -0.16946074911143683 1111 3 -0.14692117669745386 1111 4 -0.18162792255987625 1111 5 -0.20537087231549833 1111 6 -0.2221394325140551 1111 7 -0.10975861994641178 1111 8 -0.1506722908600011 1111 9 -0.13574409447779345 1111 10-11 -0.14592213816717603 1111 12-13 0.04132779252686447 1111 14-15 -0.06300544556425791 1111 16-17 -0.09879443662354603 1111 18-19 0.004145505416040862 1111 20-21 0.09677868289872293 1111 22-23 5.446782316731259 1111 24-25 4.152698270759437 1111 26-27 1.3592732729828647 1111 28-29 0.6424278980125635 1111 30-31 0.4110388562264653 1111 32-33 0.3713020778806211 1111 34-35 0.3704013763427163 1111 36-37 0.23223107950521538 1111 38-39 0.19634775565823048 1111 40-41 0.27305870228295603 1111 42-43 0.29709135501562756 1111 44-45 0.4196635446803292 1111 46-47 0.3421700911446912 1111 48-49 0.30462850095022276 1111 50-51 0.4942280356968425 1111 52-53 0.3617023447024259 1111 54-55 0.34908430672453505 1111 56-57 0.4158401389192541 1111 58-59 0.37822179366314046 1111 60-61 0.3629370917416743 1111 62-63 0.31588780555535934 1111 64-65 0.3208645691081422 1111 66-67 0.3123043799999472 1111 68-69 0.3953869372858634 1111 70-71 0.39212566092593093 1111 72-73 0.4180251870018701 1111 74-75 0.4940966086118088 1111 76-77 0.5178394096369168 1111 78-79 0.49898941446961587 1111 80-81 0.503234081333602 1111 82-83 0.4290673451563052 1111 84-85 0.5295504272595899 1111 86-87 0.5012682973601699 1111 88-89 0.4916742095156259 1111 90-91 0.4139934533222096 1111 92-93 0.40997820174217736 1111 94-95 0.514394024122339 1111 96-97 0.4966364044573872 1111 98-99 0.5609441976056857 1111 100-101 0.46307895239130303 1113 1 -0.4285630385775576 1113 2 -0.3249161414838433 1113 3 -0.3967252244367998 1113 4 -0.5002296679803528 1113 5 -0.3923031291252812 1113 6 -0.8931190808432419 1113 7 -0.8487476630115118 1113 8 -1.2722905926343522 1113 9 -0.7011245340242986 1113 10-11 -0.8577831088573085 1113 12-13 -0.5554516272177814 1113 14-15 -0.5650104747973188 1113 16-17 -0.7609209628897453 1113 18-19 -0.5678971235036983 1113 20-21 -0.5576692385322275 1113 22-23 -9.825793302687472 1113 24-25 -8.035594202911941 1113 26-27 -2.798724558961954 1113 28-29 -1.2686296909028982 1113 30-31 -0.9985325704677059 1113 32-33 -0.6008839955981458 1113 34-35 -0.7507255138336895 1113 36-37 -0.49973261335147257 1113 38-39 -0.35753669056494175 1113 40-41 -0.35039415140105845 1113 42-43 -0.519384787891255 1113 44-45 -0.5341446718736513 1113 46-47 -0.32533483594501433 1113 48-49 -0.430380175163549 1113 50-51 -0.11579478551147204 1113 52-53 -0.12395253624160674 1113 54-55 -0.26137020774198305 1113 56-57 -0.38094913511680417 1113 58-59 -0.18002902460420955 1113 60-61 -0.19341267622118608 1113 62-63 -0.37627108690388056 1113 64-65 -0.3003758113012083 1113 66-67 -0.25386070068100963 1113 68-69 -0.15421159884366986 1113 70-71 -0.17867115127601707 1113 72-73 -0.20212728921635303 1113 74-75 -0.19207042203413138 1113 76-77 -0.045640881232372976 1113 78-79 -0.2981885016390713 1113 80-81 -0.3424803438129018 1113 82-83 -0.15821599602669423 1113 84-85 -0.1568776951840647 1113 86-87 -0.045797631344363765 1113 88-89 0.10125471674447084 1113 90-91 0.027903725804591772 1113 92-93 0.06569204403238516 1113 94-95 0.10653906929938017 1113 96-97 0.12137028265751937 1113 98-99 0.013517257597662535 1113 100-101 0.06796497610099195 1114 1 -0.16546773075051036 1114 2 -0.22404480884553735 1114 3 -0.2314601192179495 1114 4 -0.2821264464279487 1114 5 -0.09182128166655801 1114 6 -0.3176849684708323 1114 7 -0.17854084259610303 1114 8 -0.16896998404130414 1114 9 -0.3069601401794273 1114 10-11 -0.07261947943461067 1114 12-13 -0.059477632876841824 1114 14-15 -0.2785947985112003 1114 16-17 -0.09785012162346263 1114 18-19 0.030479389445503102 1114 20-21 -0.10096946465401047 1114 22-23 5.428476199617798 1114 24-25 4.114608926710083 1114 26-27 1.359251116542378 1114 28-29 0.7160197752180579 1114 30-31 0.3966229223735418 1114 32-33 0.38477068210492504 1114 34-35 0.20152677007790842 1114 36-37 0.3504501390871191 1114 38-39 0.26428970936238017 1114 40-41 0.3437951410904283 1114 42-43 0.4406128807680041 1114 44-45 0.3373301470828842 1114 46-47 0.4845181067886024 1114 48-49 0.5561699408350407 1114 50-51 0.5574386030677765 1114 52-53 0.5253412283127155 1114 54-55 0.5164941854478258 1114 56-57 0.624817357455381 1114 58-59 0.5862307000845526 1114 60-61 0.5464276861582107 1114 62-63 0.5494923459754091 1114 64-65 0.45821574202983584 1114 66-67 0.5425588557064494 1114 68-69 0.6143484070619856 1114 70-71 0.605121653897033 1114 72-73 0.6943791217885611 1114 74-75 0.7192675770214869 1114 76-77 0.5552565808593464 1114 78-79 0.6584832929701925 1114 80-81 0.6366324838769053 1114 82-83 0.6777862803206283 1114 84-85 0.6538548353017113 1114 86-87 0.5485592278111397 1114 88-89 0.653666545351399 1114 90-91 0.5723142480072596 1114 92-93 0.4567696014103646 1114 94-95 0.5639098769385917 1114 96-97 0.5764630826708697 1114 98-99 0.5696388251750228 1114 100-101 0.6279305620623994 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 268.0 3 333.0 4 355.0 5 337.0 6 363.0 7 1040.0 8 5485.0 9 22295.0 10 70066.0 11 145257.0 12 205358.0 13 225188.0 14 220811.0 15 210902.0 16 204750.0 17 201891.0 18 200650.0 19 200671.0 20 204434.0 21 210309.0 22 218951.0 23 229173.0 24 239166.0 25 244117.0 26 243979.0 27 248082.0 28 261628.0 29 280112.0 30 302087.0 31 333420.0 32 376718.0 33 429752.0 34 500106.0 35 560465.0 36 557944.0 37 439891.0 38 186419.0 39 17215.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 11.990988458186681 49.579176819338315 21.71667418129181 16.713160541183193 2 16.230971533028203 51.1413828415744 16.520153703537872 16.10749192185953 3 44.05003866474012 23.429031535494833 16.807211715059815 15.713718084705233 4 15.853026403052258 23.3484051621806 16.817739360763994 43.98082907400315 5 15.813920968356024 50.09109356809116 18.672417873200956 15.422567590351862 6 42.57858325124138 24.895801559163715 16.658986693602827 15.86662849599208 7 16.191085067128654 23.69643753223793 45.100909875313754 15.011567525319666 8 42.610042116246035 24.603368574597162 17.54415617552632 15.242433133630476 9 41.950403920300325 24.760822599984323 17.789270355629135 15.49950312408621 10-11 15.274793753022703 38.00657707497626 31.31016611211782 15.408463059883212 12-13 14.982848945443944 25.179193387829628 30.897090922093607 28.940866744632824 14-15 15.249679482150427 51.166026089393426 17.841609139945696 15.74268528851045 16-17 28.65585784128037 24.299997974704812 17.786817428640227 29.257326755374596 18-19 15.552968039783648 37.14015079770206 18.11485869575953 29.192022466754757 20-21 28.96969521947863 37.02723789792777 18.19075054613063 15.812316336462972 22-23 31.298235662754585 22.675239377226248 28.382350387209904 17.64417457280927 24-25 23.923166483559733 31.047356873843523 22.243919694193394 22.785556948403357 26-27 25.718350935464795 27.37096488152388 23.73214987965572 23.178534303355605 28-29 40.983485313957836 22.463492766072374 20.32483592219292 16.22818599777687 30-31 33.08600498566037 26.887688232122343 22.320773324372688 17.7055334578446 32-33 23.70183643633871 30.237848007839858 26.063588621857292 19.996726933964144 34-35 22.221360900410264 28.740449382430178 25.438035279380216 23.60015443777934 36-37 24.944417937842502 27.20092555176752 26.774844864476304 21.079811645913676 38-39 24.96681440620217 26.905368272715396 25.95785868811069 22.16995863297174 40-41 21.536560871548996 29.684144088386955 27.224313341149788 21.554981698914265 42-43 21.835502046444606 28.05123596449903 27.480961568211754 22.632300420844608 44-45 21.62908864423648 30.532470744422234 25.270134344126948 22.568306267214336 46-47 23.564441634452336 26.948651876060588 25.779061452213508 23.707845037273565 48-49 22.622636693442438 28.53475024696232 25.495759901722813 23.346853157872424 50-51 23.533025973450272 29.891504116246136 25.115988351427653 21.45948155887594 52-53 24.020590306383156 26.793675734434963 27.02050071426736 22.16523324491452 54-55 23.210547018344823 28.34326104394368 26.45590704043887 21.990284897272627 56-57 23.630278104737243 28.16753492665872 25.158935435954838 23.0432515326492 58-59 26.257400255629992 26.90093934611717 25.17498362376237 21.66667677449047 60-61 24.068655514717857 27.901831306804176 25.041643343914092 22.987869834563874 62-63 24.232248039564723 28.870159779742476 25.074332662941934 21.823259517750866 64-65 22.642726460834876 27.88430798175928 26.93641366271121 22.536551894694632 66-67 24.257029212264424 28.29849918716053 26.108274931500585 21.336196669074457 68-69 25.980922204706925 27.661971792247336 25.21631265390738 21.140793349138356 70-71 22.447581685225423 28.378932225371717 26.95745282854914 22.216033260853717 72-73 23.17548468252136 27.24845760598995 26.138295250074723 23.43776246141397 74-75 23.39744405202963 29.328958168992227 25.877146290473547 21.396451488504596 76-77 23.885302892878496 26.73752634794314 26.46688867833041 22.910282080847956 78-79 22.868714592970473 27.724355398046164 25.287989831817804 24.11894017716556 80-81 24.16440598703522 27.75172746096104 26.1874186294421 21.89644792256164 82-83 24.151640866176287 27.279214194708366 26.590753084243257 21.978391854872083 84-85 25.61257035905275 27.084613128656088 25.310751180786983 21.99206533150418 86-87 23.55708076761811 27.33890734242947 25.931970482420414 23.172041407532006 88-89 25.325505584806162 26.607950426676304 25.105983832062577 22.960560156454957 90-91 24.061764206385654 27.64731623805971 25.111352926097812 23.179566629456826 92-93 23.40641574052614 28.843261294764083 26.395155041812274 21.355167922897504 94-95 22.45311644693838 28.079824168196637 27.05884353246098 22.408215852404002 96-97 24.26749678155879 27.17281972953843 26.04589650933204 22.513786979570742 98-99 25.34755671841722 27.2729170772824 25.68591345661276 21.693612747687627 100-101 22.77848614335644 29.227507642283012 26.07482441224101 21.919181802119546 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 56659.0 1 40671.5 2 18851.0 3 11612.5 4 9710.0 5 8863.5 6 8454.0 7 8439.5 8 8528.0 9 8897.0 10 9551.5 11 11513.5 12 42956.0 13 45240.5 14 15887.5 15 13716.0 16 13578.0 17 14237.0 18 15317.5 19 16713.0 20 18723.5 21 21436.5 22 24674.5 23 28364.5 24 32972.5 25 38647.5 26 45093.5 27 52152.0 28 59130.5 29 67037.5 30 76254.5 31 86462.0 32 97510.0 33 109821.0 34 124261.0 35 139097.5 36 152846.0 37 162971.5 38 168764.0 39 172986.0 40 178788.5 41 187662.5 42 196490.0 43 205050.5 44 215790.0 45 226286.0 46 236622.5 47 246991.0 48 254465.5 49 260771.0 50 268922.5 51 280189.5 52 299278.0 53 335811.0 54 339106.0 55 309629.0 56 291387.0 57 268134.0 58 236647.0 59 203891.5 60 173849.5 61 144720.5 62 118283.0 63 95928.0 64 78091.5 65 63876.5 66 51980.0 67 42289.5 68 33996.0 69 26869.0 70 21451.0 71 17427.5 72 14318.0 73 11469.5 74 8830.5 75 6707.0 76 5036.0 77 3721.0 78 2693.5 79 1899.0 80 1340.5 81 926.0 82 639.5 83 457.0 84 324.0 85 227.0 86 149.0 87 110.0 88 76.5 89 41.0 90 25.0 91 16.0 92 16.5 93 12.0 94 4.0 95 2.5 96 1.5 97 3.0 98 2.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.139175 2 0.05435 3 0.054287499999999995 4 0.067425 5 0.06155000000000001 6 0.0105 7 0.011025 8 0.0118625 9 0.0127625 10-11 0.01444375 12-13 0.020725 14-15 0.02019375 16-17 0.014575 18-19 0.0167125 20-21 0.012475 22-23 15.068675 24-25 0.029956250000000004 26-27 0.01861875 28-29 0.028374999999999997 30-31 0.025781250000000002 32-33 0.01729375 34-35 0.024462499999999998 36-37 0.03800625 38-39 0.03663125 40-41 0.045206249999999996 42-43 0.049212500000000006 44-45 0.053781249999999996 46-47 0.05903125 48-49 0.0480125 50-51 0.03710625 52-53 0.03305625 54-55 0.04094375 56-57 0.031143749999999998 58-59 0.03618125 60-61 0.032793749999999997 62-63 0.0397375 64-65 0.0231375 66-67 0.03791875 68-69 0.015962499999999998 70-71 0.0236125 72-73 0.02250625 74-75 0.01899375 76-77 0.027775 78-79 0.01654375 80-81 0.01854375 82-83 0.026125 84-85 0.008425 86-87 0.02483125 88-89 0.012756249999999998 90-91 0.009643750000000001 92-93 0.008425 94-95 0.01524375 96-97 0.009825 98-99 0.01676875 100-101 0.0339625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8000000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.51363289887192 #Duplication Level Percentage of deduplicated Percentage of total 1 91.64135963804085 69.20171990060516 2 4.440691526717684 6.706654995313806 3 1.3171521203261343 2.983888250588354 4 0.7125120845827552 2.152175039647686 5 0.47334767890321966 1.7872101429115415 6 0.32070932855155154 1.4530755902091337 7 0.23729993461251161 1.2543566104478856 8 0.17329526797695177 1.0468924199298537 9 0.12995384755032308 0.8831958423939957 >10 0.5190477478131362 6.25516032781951 >50 0.019083894060651474 1.0068703740638572 >100 0.013718844581490553 1.8684544005633827 >500 0.0012418115449079321 0.6494829910021092 >1k 5.000578730116348E-4 0.5055311307554063 >5k 1.7243372943149233E-5 0.10156455928065032 >10k+ 6.897349177259693E-5 2.143767424467655 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 64722 0.809025 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49846 0.623075 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA 37247 0.46558750000000004 No Hit AAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAA 12823 0.1602875 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.25E-5 0.0 2 0.0 0.0 0.0 1.25E-5 0.0 3 1.25E-5 0.0 0.0 2.5E-5 0.0 4 2.5E-5 0.0 0.0 3.75E-5 0.0 5 2.5E-5 0.0 0.0 3.75E-5 1.25E-5 6 2.5E-5 0.0 0.0 5.0E-5 1.25E-5 7 3.75E-5 0.0 0.0 6.25E-5 1.25E-5 8 5.0E-5 0.0 0.0 6.25E-5 1.25E-5 9 6.25E-5 0.0 0.0 6.25E-5 1.25E-5 10-11 6.25E-5 2.4999999999999998E-5 0.0 7.5E-5 1.25E-5 12-13 6.25E-5 5.0E-5 0.0 7.5E-5 1.25E-5 14-15 6.25E-5 8.125E-5 0.0 8.125E-5 1.25E-5 16-17 7.5E-5 1.0E-4 0.0 8.75E-5 1.25E-5 18-19 9.999999999999999E-5 1.0E-4 0.0 9.375E-5 1.25E-5 20-21 1.1875E-4 1.0E-4 0.0 1.125E-4 1.25E-5 22-23 1.3749999999999998E-4 1.0E-4 1.25E-5 1.25E-4 1.25E-5 24-25 1.9375000000000002E-4 1.0E-4 1.25E-5 1.3125000000000002E-4 1.25E-5 26-27 2.25E-4 1.0E-4 1.25E-5 1.375E-4 2.5E-5 28-29 2.5E-4 1.0E-4 1.25E-5 1.5E-4 2.5E-5 30-31 3.0625E-4 1.0E-4 1.25E-5 1.5E-4 2.5E-5 32-33 3.9375E-4 1.0E-4 1.25E-5 1.5E-4 2.5E-5 34-35 4.875E-4 1.1875E-4 1.25E-5 1.5E-4 3.75E-5 36-37 5.9375E-4 1.25E-4 1.25E-5 1.75E-4 4.375E-5 38-39 6.187499999999999E-4 1.3125000000000002E-4 1.25E-5 1.875E-4 6.25E-5 40-41 6.5E-4 1.375E-4 1.25E-5 1.875E-4 8.75E-5 42-43 7.25E-4 1.375E-4 1.25E-5 1.9375000000000002E-4 8.75E-5 44-45 8.1875E-4 1.375E-4 1.25E-5 2.0E-4 1.0E-4 46-47 9.125E-4 1.4375E-4 1.25E-5 2.0E-4 1.1875E-4 48-49 0.001075 1.5E-4 1.25E-5 2.0625E-4 1.25E-4 50-51 0.0011749999999999998 1.625E-4 1.25E-5 2.4375E-4 1.25E-4 52-53 0.0013062500000000001 1.6875E-4 1.25E-5 2.5E-4 1.25E-4 54-55 0.001425 1.875E-4 1.25E-5 2.5E-4 1.3125000000000002E-4 56-57 0.00161875 1.875E-4 1.25E-5 2.625E-4 1.375E-4 58-59 0.001775 1.9375000000000002E-4 1.25E-5 3.0000000000000003E-4 1.5000000000000001E-4 60-61 0.00191875 2.0E-4 1.25E-5 3.25E-4 1.625E-4 62-63 0.00206875 2.0E-4 1.25E-5 3.3125E-4 1.75E-4 64-65 0.00220625 2.0E-4 1.25E-5 3.5E-4 1.75E-4 66-67 0.002425 2.0625E-4 1.25E-5 3.75E-4 1.875E-4 68-69 0.00273125 2.125E-4 1.25E-5 4.3125E-4 2.0E-4 70-71 0.003125 2.125E-4 1.25E-5 4.375E-4 2.0E-4 72-73 0.00354375 2.125E-4 1.25E-5 4.375E-4 2.25E-4 74-75 0.0044062500000000004 2.125E-4 2.5E-5 4.4375E-4 2.3125E-4 76-77 0.00559375 2.125E-4 2.5E-5 4.5625E-4 2.5E-4 78-79 0.007062499999999999 2.125E-4 2.5E-5 4.625E-4 2.5E-4 80-81 0.0090875 2.125E-4 2.5E-5 4.625E-4 2.5E-4 82-83 0.012275000000000001 2.25E-4 2.5E-5 4.6875E-4 2.5E-4 84-85 0.016575 2.25E-4 2.5E-5 4.875E-4 2.5E-4 86-87 0.0226125 2.5625E-4 2.5E-5 4.875E-4 2.5E-4 88-89 0.030543749999999998 2.75E-4 2.5E-5 4.875E-4 2.5E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGTAT 254070 0.0 72.77724 6 TGGTATC 253220 0.0 72.583694 7 AGTGGTA 255125 0.0 72.36507 5 CAGTGGT 257200 0.0 71.9632 4 GTATCAA 255710 0.0 71.92953 9 GGTATCA 256705 0.0 71.747086 8 AAGCAGT 269935 0.0 69.94822 1 AGCAGTG 274925 0.0 68.666245 2 GCAGTGG 277055 0.0 67.97984 3 GAGTACT 13785 0.0 50.559887 20-21 GTCACCG 8635 0.0 49.9238 22-23 GTACTTT 12570 0.0 48.2567 22-23 TCACCGG 9025 0.0 48.061054 22-23 AGAGTAC 23135 0.0 47.04059 18-19 AGTACTT 13330 0.0 46.625763 20-21 CAGAGTA 59015 0.0 46.48208 18-19 CAGAGTG 57680 0.0 44.809864 18-19 AGAGTGG 24770 0.0 43.774582 18-19 GAGTCAC 12435 0.0 43.219395 20-21 AGAGTCA 22650 0.0 42.663563 18-19 >>END_MODULE