FastQCFastQC Report
Sat 18 Jun 2016
SRR799686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR799686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8000000
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3448474.3105875000000005No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1205871.5073375No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA376070.4700875No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA81740.10217500000000002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2360750.070.410469
TGGTATC2368950.070.135127
GTGGTAT2357200.070.102056
GGTATCA2373400.070.027288
AGTGGTA2400350.068.738175
CAGTGGT2415850.068.112884
AAGCAGT2448300.066.8527451
AGCAGTG2500250.065.6149442
GCAGTGG2529450.065.094623
AGAGTAC639600.043.51174518-19
GAGTACT542650.042.96478320-21
AGTACTT532200.042.64723220-21
GTACTTT522500.042.6421722-23
TAACGCC100300.041.3557554-55
CTTAACG100350.041.31181752-53
TACTTTT563600.039.75681722-23
ACGCAGA2152950.039.5533114-15
AGACTCG101900.039.4431866-67
CAACGCA2165550.039.39787712-13
AACGCCC106100.039.35976854-55
Produced by FastQC (version 0.11.5)