##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR799686_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8000000 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.86567 31.0 31.0 33.0 28.0 34.0 2 31.164584875 31.0 31.0 34.0 28.0 34.0 3 31.460022125 31.0 31.0 34.0 30.0 34.0 4 34.852520125 35.0 35.0 37.0 32.0 37.0 5 34.826902375 35.0 35.0 37.0 32.0 37.0 6 34.4556955 35.0 35.0 37.0 30.0 37.0 7 34.585564375 35.0 35.0 37.0 32.0 37.0 8 34.633786875 35.0 35.0 37.0 32.0 37.0 9 36.07455475 38.0 35.0 39.0 31.0 39.0 10-11 36.14854875 37.5 35.0 39.0 31.5 39.0 12-13 35.946996687500004 38.0 35.0 39.0 30.0 39.0 14-15 37.1327058125 39.0 36.0 40.0 32.0 41.0 16-17 35.496246812500004 38.0 34.5 40.0 26.5 41.0 18-19 33.8917786875 38.0 33.0 40.0 10.0 41.0 20-21 36.1629476875 38.0 34.0 40.0 29.0 41.0 22-23 36.514129187500004 38.5 35.0 40.0 30.0 41.0 24-25 35.239275125 38.0 33.5 40.0 25.5 41.0 26-27 33.761937625 37.0 31.5 40.0 19.5 41.0 28-29 33.907305 37.0 32.0 40.0 20.5 41.0 30-31 33.477345687500005 37.0 31.0 40.0 17.5 41.0 32-33 32.964338062500005 37.0 30.0 40.0 16.0 41.0 34-35 32.965897625 37.0 30.0 40.0 16.0 41.0 36-37 32.8883390625 37.0 30.0 39.5 16.0 41.0 38-39 32.49512581250001 35.0 30.0 39.0 16.0 41.0 40-41 32.432285875000005 35.0 30.0 39.0 15.5 41.0 42-43 32.339625 35.0 30.0 39.0 15.5 41.0 44-45 31.047814062500002 35.0 27.0 39.0 11.0 41.0 46-47 31.743565250000003 35.0 28.5 39.0 15.0 40.5 48-49 31.8159368125 35.0 28.5 39.0 14.5 40.0 50-51 31.66936425 35.0 28.0 39.0 14.0 40.0 52-53 31.490205625 35.0 28.0 39.0 13.0 40.0 54-55 31.17277575 35.0 27.5 39.0 13.0 40.0 56-57 30.8938644375 35.0 27.0 38.5 11.5 40.0 58-59 30.5888766875 34.0 26.5 38.0 10.5 40.0 60-61 30.2398325625 34.0 26.0 38.0 10.0 40.0 62-63 30.1476131875 34.0 26.0 37.5 10.0 40.0 64-65 29.763706562499998 34.0 26.0 37.0 10.0 40.0 66-67 29.518506062500002 33.5 26.0 36.5 10.0 39.0 68-69 29.2821765625 33.0 26.0 36.0 10.0 39.0 70-71 29.0352495 33.0 25.0 36.0 10.0 39.0 72-73 28.8894471875 33.0 25.0 35.0 10.0 38.5 74-75 28.5887256875 32.5 25.0 35.0 10.0 37.5 76-77 28.355334687499997 32.0 25.0 35.0 9.0 37.0 78-79 28.217103625 32.0 25.0 35.0 8.0 36.5 80-81 28.025621375 32.0 24.0 35.0 8.0 36.0 82-83 27.930060625 32.0 24.0 35.0 8.0 36.0 84-85 27.668230625 32.0 24.0 35.0 8.0 35.5 86-87 27.413721375 32.0 23.5 35.0 8.0 35.0 88-89 27.4016060625 32.0 24.0 35.0 8.0 35.0 90-91 27.3426715625 32.0 24.0 35.0 8.0 35.0 92-93 27.269984125 32.0 23.5 34.0 8.0 35.0 94-95 27.257593937499998 32.0 24.0 34.0 7.5 35.0 96-97 27.1374183125 32.0 23.0 34.0 7.0 35.0 98-99 27.063655812500002 31.5 23.0 34.0 7.0 35.0 100-101 25.528034249999997 29.0 20.5 33.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.00830895872319104 1101 2 -0.5092783921367356 1101 3 -0.07563634463316049 1101 4 0.041790091269923835 1101 5 -0.1929717324000677 1101 6 0.4222144970959718 1101 7 0.25073084302729143 1101 8 0.22770333318837288 1101 9 0.3672254996073292 1101 10-11 0.32787912337877145 1101 12-13 0.42091300440498713 1101 14-15 0.36138703282628626 1101 16-17 2.8604194243095407 1101 18-19 5.3860007028547265 1101 20-21 1.4224854335429669 1101 22-23 0.7656599655864156 1101 24-25 0.3326373138793457 1101 26-27 0.4746954164382018 1101 28-29 0.541316026762992 1101 30-31 0.5226765685836625 1101 32-33 0.5262851124757901 1101 34-35 0.5593155508262058 1101 36-37 0.43549030723686855 1101 38-39 0.4196904588421475 1101 40-41 0.5384473152483835 1101 42-43 0.5022025789092552 1101 44-45 2.6095928825635717 1101 46-47 1.0615228973189232 1101 48-49 0.5914555910041663 1101 50-51 0.39180909816868237 1101 52-53 0.34005564039480163 1101 54-55 0.1779114281061034 1101 56-57 0.19566461363936227 1101 58-59 0.27381545383175165 1101 60-61 0.3599923418390638 1101 62-63 0.2398424982138465 1101 64-65 0.25609877436739126 1101 66-67 0.2536688801888971 1101 68-69 0.2316744955231833 1101 70-71 0.24471115012103084 1101 72-73 0.25405056381960023 1101 74-75 0.3254188699628848 1101 76-77 0.21358299641758904 1101 78-79 0.139378221687533 1101 80-81 0.23044209720261577 1101 82-83 0.23172007001280903 1101 84-85 0.26847633554226036 1101 86-87 0.3298826739753018 1101 88-89 0.2533220209716909 1101 90-91 0.16870790609682373 1101 92-93 0.21297677145683025 1101 94-95 0.15000438346425327 1101 96-97 0.13271635651631897 1101 98-99 0.1496753513353397 1101 100-101 0.14948220266639467 1102 1 -0.3955418839971472 1102 2 -0.35552095672091255 1102 3 -0.15365687074472945 1102 4 -1.383339489746561 1102 5 -0.8420508607243775 1102 6 -0.3428239768468728 1102 7 -0.4539171025349731 1102 8 -0.39587132661943514 1102 9 -0.2912651895161389 1102 10-11 -0.34790878188911734 1102 12-13 -0.20320164909801974 1102 14-15 -0.43491545139495 1102 16-17 2.0882266768906277 1102 18-19 4.576074199474984 1102 20-21 0.5621957625557528 1102 22-23 0.032707578676507865 1102 24-25 -0.37171029748102313 1102 26-27 -0.18332693530490474 1102 28-29 -0.061545851746558355 1102 30-31 -0.1406124272096747 1102 32-33 -0.19551472654326574 1102 34-35 -0.1571400668634979 1102 36-37 -0.21450452887616223 1102 38-39 -0.24169245886605495 1102 40-41 -0.12253827690470587 1102 42-43 -0.18028421790886995 1102 44-45 1.937459658745258 1102 46-47 0.4258803640914621 1102 48-49 -0.02919899341304344 1102 50-51 -0.19688026539754233 1102 52-53 -0.26520717883940037 1102 54-55 -0.3569182194427114 1102 56-57 -0.4133348843291209 1102 58-59 -0.3609211972491231 1102 60-61 -0.3299834807757449 1102 62-63 -0.43201471235956745 1102 64-65 -0.3246066402196952 1102 66-67 -0.3668046539627099 1102 68-69 -0.3465100773609322 1102 70-71 -0.33386614995630737 1102 72-73 -0.4187539622961083 1102 74-75 -0.32295166545366527 1102 76-77 -0.3645231815143539 1102 78-79 -0.395735813881398 1102 80-81 -0.4394959096563049 1102 82-83 -0.3707521044927624 1102 84-85 -0.28230563337487524 1102 86-87 -0.26453157813526573 1102 88-89 -0.3141126325488415 1102 90-91 -0.3707168568153314 1102 92-93 -0.31787288322317053 1102 94-95 -0.34239105999126096 1102 96-97 -0.34980798728284057 1102 98-99 -0.39412541790229483 1102 100-101 -0.4311608692456197 1103 1 0.11644038923793332 1103 2 0.2521340451940901 1103 3 0.1913138438177846 1103 4 0.11765755261066602 1103 5 -0.08744125896555488 1103 6 0.36111304265126876 1103 7 0.2207862652308208 1103 8 0.1344560358582143 1103 9 0.19142173516620886 1103 10-11 0.2309565130287936 1103 12-13 0.3420298537804527 1103 14-15 0.14491346103935854 1103 16-17 2.4754806965691927 1103 18-19 5.104220503139839 1103 20-21 1.2099953731206412 1103 22-23 0.5688072898965189 1103 24-25 0.06728516324857026 1103 26-27 -0.029963482784076234 1103 28-29 -0.06857873130398673 1103 30-31 0.01857088042117283 1103 32-33 -0.02780341149686194 1103 34-35 -0.04747346487944526 1103 36-37 0.0493870597912931 1103 38-39 0.01879120675020829 1103 40-41 0.19896292149069694 1103 42-43 0.19106847482829892 1103 44-45 2.244416271830989 1103 46-47 0.7554264830873976 1103 48-49 0.26803363701118244 1103 50-51 0.17569376236158618 1103 52-53 0.06950715999111523 1103 54-55 0.03282693418193361 1103 56-57 0.016420012036082454 1103 58-59 0.08974799145587298 1103 60-61 0.1457853503119253 1103 62-63 0.06555543988430657 1103 64-65 0.07331951252305302 1103 66-67 0.062339732361021305 1103 68-69 0.10246079964434784 1103 70-71 0.13584498136361844 1103 72-73 0.07610050392067436 1103 74-75 0.15543154665947156 1103 76-77 0.11315260609081434 1103 78-79 0.09809485474112023 1103 80-81 0.07566079099457568 1103 82-83 0.12172274978972553 1103 84-85 0.17364735612613913 1103 86-87 0.15170715632337917 1103 88-89 0.07758467470118902 1103 90-91 0.04569651085769166 1103 92-93 0.04273573852838197 1103 94-95 0.03279343273459645 1103 96-97 0.04909374688470436 1103 98-99 0.041457872390367356 1103 100-101 0.06427590761584767 1104 1 0.20466612051884425 1104 2 0.3372808612298819 1104 3 0.27939868248726185 1104 4 0.2109680020516791 1104 5 0.16386413630113594 1104 6 0.4936478521966592 1104 7 -0.4799411488947456 1104 8 -0.13697618294108338 1104 9 0.06598422253953373 1104 10-11 -0.5904598166701405 1104 12-13 -0.4126698930101824 1104 14-15 -0.027648154733086017 1104 16-17 2.6877862979114013 1104 18-19 5.275555104117462 1104 20-21 1.202389047068003 1104 22-23 0.0021456999137186017 1104 24-25 -0.04920636123115685 1104 26-27 -0.4344416957195065 1104 28-29 -1.2872089669032505 1104 30-31 -1.3538575499521954 1104 32-33 -1.3910277494420598 1104 34-35 -1.3628266612289295 1104 36-37 -1.6118557802676463 1104 38-39 -1.5349756289943812 1104 40-41 -1.4747726947515005 1104 42-43 -1.595612043551398 1104 44-45 0.4665971530256492 1104 46-47 -0.9405979649895997 1104 48-49 -1.4042670508192998 1104 50-51 -1.7483798991074302 1104 52-53 -1.6434193777934567 1104 54-55 -1.3353407152301564 1104 56-57 -0.8405056060844522 1104 58-59 -1.526055007423519 1104 60-61 -1.7932960309101702 1104 62-63 -1.2616223037545495 1104 64-65 -1.7612934961490865 1104 66-67 -1.567177436595916 1104 68-69 -1.6391168861193073 1104 70-71 -1.7288831384647416 1104 72-73 -1.425001878505558 1104 74-75 -1.5355063588815483 1104 76-77 -1.8164684708982328 1104 78-79 -1.2626343726430527 1104 80-81 -1.5283665634951724 1104 82-83 -1.5293044625746397 1104 84-85 -1.5918922011971546 1104 86-87 -1.6380210489838802 1104 88-89 -1.750498869256397 1104 90-91 -1.728449804744674 1104 92-93 -1.7226531505395393 1104 94-95 -1.7732469216921594 1104 96-97 -1.8249692124462094 1104 98-99 -1.7589539706276796 1104 100-101 -1.8373688825573922 1105 1 -7.811060101694522E-4 1105 2 0.02960218308407292 1105 3 -0.0975697558634927 1105 4 0.09490690232739496 1105 5 0.15180925803483802 1105 6 -3.472217441594008 1105 7 -0.6386041211375826 1105 8 -0.22586275564949432 1105 9 0.16480287551647166 1105 10-11 0.2776010334001029 1105 12-13 0.3232722005740385 1105 14-15 0.270262422587777 1105 16-17 -24.960539673519197 1105 18-19 -0.4148290349548347 1105 20-21 0.31779771759928366 1105 22-23 -2.4081437205902816 1105 24-25 0.18443530092986293 1105 26-27 0.623766447860632 1105 28-29 0.6879834220717029 1105 30-31 0.6711369976803354 1105 32-33 0.722474855497282 1105 34-35 0.890844581426613 1105 36-37 1.0226944800753373 1105 38-39 1.1026212656873469 1105 40-41 0.9590684460116847 1105 42-43 0.9603732614012515 1105 44-45 2.98473404395806 1105 46-47 1.4552711779019631 1105 48-49 1.0484784460754533 1105 50-51 0.9210787634264186 1105 52-53 0.8638968961358167 1105 54-55 0.8443059610632666 1105 56-57 0.7712423723240249 1105 58-59 0.749442763510654 1105 60-61 0.7947420316454199 1105 62-63 0.7527995570796904 1105 64-65 0.8787905533971063 1105 66-67 0.863735699207794 1105 68-69 0.7252447206908315 1105 70-71 0.7049975040256378 1105 72-73 0.7036004778799629 1105 74-75 0.7115541804162575 1105 76-77 0.6606713650798923 1105 78-79 0.7457102962458713 1105 80-81 0.693203195764486 1105 82-83 0.6522598225292136 1105 84-85 0.5787318260297845 1105 86-87 0.7137720427609899 1105 88-89 0.5484442540925407 1105 90-91 0.516158299208282 1105 92-93 0.5010257040720596 1105 94-95 0.5985770450691454 1105 96-97 0.5344326754733224 1105 98-99 0.46207723706297443 1105 100-101 0.3321247255000195 1106 1 -0.5942441957684252 1106 2 -0.6147842851937568 1106 3 -0.35942583719666743 1106 4 -0.18465642865245968 1106 5 -0.49355986596517454 1106 6 0.04868808878447339 1106 7 -0.057188662901751286 1106 8 -0.10156723449622973 1106 9 -0.19435782520820055 1106 10-11 -0.10175311991266511 1106 12-13 -0.6068143744349115 1106 14-15 -0.43465017412531637 1106 16-17 1.8212189678684112 1106 18-19 4.7444601157562225 1106 20-21 0.29969590896154585 1106 22-23 0.17325819504277717 1106 24-25 -0.3443216269343594 1106 26-27 -0.6378850272188217 1106 28-29 -0.3585141116861408 1106 30-31 -0.1948239529916833 1106 32-33 -0.2875307721600251 1106 34-35 -0.28996340769841566 1106 36-37 -0.278409511754937 1106 38-39 -0.2364142064988357 1106 40-41 -0.8512119123631834 1106 42-43 -0.885290578132679 1106 44-45 1.5457598683099896 1106 46-47 0.32131182937667546 1106 48-49 -0.6833416913960768 1106 50-51 -0.5661849223415594 1106 52-53 -0.35856705640771125 1106 54-55 -0.35355491191859656 1106 56-57 -0.3419452314504632 1106 58-59 -0.35591436330463466 1106 60-61 -0.8289188961196032 1106 62-63 -0.6044896799802899 1106 64-65 -1.0031249810863123 1106 66-67 -1.0566021290458174 1106 68-69 -0.8533875725899378 1106 70-71 -0.9785406994001669 1106 72-73 -0.8897400915192506 1106 74-75 -0.9246427254836007 1106 76-77 -0.3222540166936696 1106 78-79 -0.2306471045906413 1106 80-81 -0.1578033831294512 1106 82-83 -0.6139000610622993 1106 84-85 -0.9776563394697533 1106 86-87 -1.44873090022978 1106 88-89 -0.49235828905957035 1106 90-91 -0.28754928602888086 1106 92-93 -0.25431474508490126 1106 94-95 -0.24228197683761366 1106 96-97 -0.1888243064295274 1106 98-99 -0.22194555416456296 1106 100-101 -0.23746206561845185 1107 1 0.1334945450166245 1107 2 0.1945753410014568 1107 3 0.08546440160296243 1107 4 0.16318062800996103 1107 5 0.13261191606434153 1107 6 0.4259497321764627 1107 7 0.2584433675406643 1107 8 0.15065703444533085 1107 9 0.16747203908921904 1107 10-11 0.19247375208126982 1107 12-13 0.1783063749529319 1107 14-15 0.11433188134558492 1107 16-17 1.5395646940975283 1107 18-19 4.910345370202304 1107 20-21 1.1896427063533537 1107 22-23 0.6234540784670841 1107 24-25 0.10371439285266604 1107 26-27 0.10954959361420435 1107 28-29 -0.03226568010725828 1107 30-31 -0.1256297578800627 1107 32-33 0.034901641279311946 1107 34-35 0.14328731869652955 1107 36-37 0.06995130484589396 1107 38-39 0.057874986423286146 1107 40-41 0.295159930683063 1107 42-43 0.24865166852397635 1107 44-45 2.3129866781241226 1107 46-47 0.8483147076476385 1107 48-49 0.3729065449522757 1107 50-51 0.2551799246779076 1107 52-53 0.21910748356984655 1107 54-55 0.16997165784826151 1107 56-57 0.18424652416360843 1107 58-59 0.28283561930781076 1107 60-61 0.35600168126940446 1107 62-63 0.30745539961721136 1107 64-65 0.3123020024520997 1107 66-67 0.3574204792029043 1107 68-69 0.3637349358750015 1107 70-71 0.37492991834180955 1107 72-73 0.35026003160485786 1107 74-75 0.34384416104281357 1107 76-77 0.3612862915294279 1107 78-79 0.25289353966753936 1107 80-81 0.2665498531802406 1107 82-83 0.31080213623223685 1107 84-85 0.34652424936297876 1107 86-87 0.41803509578044284 1107 88-89 0.28667564596792516 1107 90-91 0.25936657225595283 1107 92-93 0.2816770908495414 1107 94-95 0.2707964617604013 1107 96-97 0.2742059380274249 1107 98-99 0.2555675387954395 1107 100-101 0.2257809806440676 1108 1 0.3054980238373517 1108 2 0.303487035238053 1108 3 0.22048062146438951 1108 4 0.450309721899103 1108 5 0.46215223314494125 1108 6 0.5127992439931646 1108 7 0.22274784800902125 1108 8 0.16326380571485544 1108 9 0.48430787307840006 1108 10-11 0.43721406596724677 1108 12-13 0.45576876598883587 1108 14-15 0.4108647810945669 1108 16-17 2.6571717371247274 1108 18-19 -22.154673973819726 1108 20-21 -4.515076250747931 1108 22-23 -0.18122756402572548 1108 24-25 0.56243574272046 1108 26-27 0.42161912858643547 1108 28-29 0.22505988212719785 1108 30-31 0.40420103890348713 1108 32-33 0.778386900783886 1108 34-35 0.5491745621243922 1108 36-37 0.7819397264012693 1108 38-39 0.8895605815098335 1108 40-41 0.6739540561538391 1108 42-43 0.8047603917679069 1108 44-45 3.1241801130768785 1108 46-47 1.7663887967176635 1108 48-49 1.2869961075198937 1108 50-51 1.2362643814442613 1108 52-53 1.170544936308449 1108 54-55 0.9564384251636646 1108 56-57 0.9427900237070759 1108 58-59 1.0800820773494522 1108 60-61 1.1019492010150174 1108 62-63 1.0529440912236545 1108 64-65 1.146570323651627 1108 66-67 1.126623269446032 1108 68-69 1.0788563690913762 1108 70-71 1.1528197604126618 1108 72-73 1.105018235674276 1108 74-75 1.1178133705714544 1108 76-77 0.9761486420798384 1108 78-79 0.7958189449947604 1108 80-81 0.8571693568006715 1108 82-83 0.9188110093285538 1108 84-85 1.010816768148814 1108 86-87 1.018900385291328 1108 88-89 1.039626543705758 1108 90-91 1.0036376136749858 1108 92-93 0.9983449381040437 1108 94-95 0.983339438285693 1108 96-97 1.0501077204236076 1108 98-99 1.019443102833705 1108 100-101 1.1524902719067747 1110 1 0.3239927542622567 1110 2 0.3229820029326085 1110 3 0.21196351023562698 1110 4 0.4667237076250146 1110 5 0.44168851637518713 1110 6 0.5962603607390804 1110 7 0.4775444532183002 1110 8 0.35097130023116563 1110 9 -0.7161693294706097 1110 10-11 -0.20232907028383806 1110 12-13 -0.6305323257333129 1110 14-15 -0.20211025763935453 1110 16-17 1.6621446183756419 1110 18-19 -22.140310323776568 1110 20-21 -4.711139002794589 1110 22-23 -0.4846812540036822 1110 24-25 0.7455256921574289 1110 26-27 0.9921535734031224 1110 28-29 1.0488713750723093 1110 30-31 0.8958749413183966 1110 32-33 0.850997660225211 1110 34-35 1.1316287242547745 1110 36-37 1.3650207809931132 1110 38-39 1.413342603139256 1110 40-41 0.7187172865211053 1110 42-43 0.8620048264731608 1110 44-45 -22.599277987593304 1110 46-47 -6.466903415778717 1110 48-49 -0.3781020178046077 1110 50-51 0.6620244939377038 1110 52-53 1.1345500078597262 1110 54-55 1.2091125070009134 1110 56-57 1.005521653319935 1110 58-59 0.9270546074247719 1110 60-61 1.056196318776216 1110 62-63 0.9219218762267971 1110 64-65 0.9735029901764669 1110 66-67 0.9833152687042244 1110 68-69 0.978659501025632 1110 70-71 0.9373579421650184 1110 72-73 0.9590252814516553 1110 74-75 0.5794297682223473 1110 76-77 0.601800356686713 1110 78-79 0.8548342656581163 1110 80-81 0.786968088810557 1110 82-83 0.7130457836600961 1110 84-85 0.8502339366667435 1110 86-87 0.9739381655911892 1110 88-89 0.7602473763184214 1110 90-91 0.8652535551876461 1110 92-93 0.854259604528778 1110 94-95 0.9862987803009311 1110 96-97 0.8973707782509805 1110 98-99 0.9500804277897643 1110 100-101 0.7186194586777468 1111 1 0.006728566372963485 1111 2 0.06685671574940955 1111 3 -0.10806078824337462 1111 4 0.03565539618124092 1111 5 0.0665516435774336 1111 6 0.34414720136402366 1111 7 0.10409333060060533 1111 8 -0.03586877813329892 1111 9 -0.059224097051476576 1111 10-11 -0.01748264324933757 1111 12-13 0.036046924377345135 1111 14-15 -0.013463160386081086 1111 16-17 2.4385784010474723 1111 18-19 4.981395806588569 1111 20-21 1.0887794604253784 1111 22-23 0.3544300415060704 1111 24-25 -0.21947938035111747 1111 26-27 -0.11270139454909867 1111 28-29 0.06808867119821826 1111 30-31 0.12778122752891363 1111 32-33 -0.005269989167800304 1111 34-35 -0.10136461611747904 1111 36-37 -0.1619919400329053 1111 38-39 -0.22683757464096743 1111 40-41 -0.013762791166207933 1111 42-43 -0.06686745780984182 1111 44-45 2.041618310538002 1111 46-47 0.4812973291296849 1111 48-49 -0.0445476394539277 1111 50-51 -0.15367717798837788 1111 52-53 -0.17137636120869715 1111 54-55 -0.1860894104421682 1111 56-57 -0.21054006828491367 1111 58-59 -0.10061572123549212 1111 60-61 -0.10166155839208102 1111 62-63 -0.15233360927840778 1111 64-65 -0.061315697346866926 1111 66-67 -0.10910331036879839 1111 68-69 0.0073639301126675605 1111 70-71 -0.09002042407799138 1111 72-73 -0.02383426039972747 1111 74-75 0.003960451431737511 1111 76-77 -0.04552970340652607 1111 78-79 -0.09989219252793902 1111 80-81 -0.26922520060438515 1111 82-83 -0.04724626939115595 1111 84-85 -0.04532368604611747 1111 86-87 0.05505933994266954 1111 88-89 0.015064720854606861 1111 90-91 -0.051347848236343197 1111 92-93 -0.0910479208475472 1111 94-95 -0.06384468053208181 1111 96-97 -0.04890626712421309 1111 98-99 -0.037478580128812666 1111 100-101 -0.14099670083082216 1113 1 -0.05014099869646671 1113 2 0.014712110437674397 1113 3 -0.06466346855986416 1113 4 0.012735391778605276 1113 5 0.05772570030324431 1113 6 0.2958618013552794 1113 7 0.04469586796530223 1113 8 -0.09430999154320574 1113 9 -0.11173028243391059 1113 10-11 -0.15252593921365332 1113 12-13 0.0285066191088319 1113 14-15 -0.21215237957986943 1113 16-17 2.359747484090164 1113 18-19 5.011591095476486 1113 20-21 1.1282826346489898 1113 22-23 0.4417239852097836 1113 24-25 -0.3882525657874538 1113 26-27 -0.5699640613627182 1113 28-29 -0.5483042116801471 1113 30-31 -0.4588636397410113 1113 32-33 -0.4623244936014288 1113 34-35 -0.6810133925473849 1113 36-37 -0.7702842841630329 1113 38-39 -0.7666622178386397 1113 40-41 -0.42372109004613634 1113 42-43 -0.41841032587641536 1113 44-45 1.6839459975630753 1113 46-47 0.22306447331234125 1113 48-49 -0.46879877619171495 1113 50-51 -0.37215003429678006 1113 52-53 -0.6877686539225429 1113 54-55 -0.507786978390893 1113 56-57 -0.4845574250644695 1113 58-59 -0.30551340326116616 1113 60-61 -0.23979392389704657 1113 62-63 -0.23579020840704956 1113 64-65 0.005000460854002853 1113 66-67 -0.08857660836069314 1113 68-69 -0.1325332710264533 1113 70-71 -0.034291602781355124 1113 72-73 -0.15932343496800527 1113 74-75 -0.029487177135557374 1113 76-77 -0.01000448176120372 1113 78-79 -0.34529084939283194 1113 80-81 -0.14172581653435046 1113 82-83 -0.121309883709948 1113 84-85 -0.10958408856284763 1113 86-87 -0.04107454104186914 1113 88-89 -0.03807201933468818 1113 90-91 -0.0233808395678885 1113 92-93 0.062489126380551596 1113 94-95 -0.08796259605341916 1113 96-97 -0.047083579173420986 1113 98-99 0.048784941505864765 1113 100-101 0.1741458231023536 1114 1 -0.04180325605061341 1114 2 -0.04204666081585273 1114 3 -0.12960799436671167 1114 4 -0.02593147535468887 1114 5 0.13962031425403154 1114 6 0.314359598084458 1114 7 0.0506090598770399 1114 8 -0.0365952400552203 1114 9 -0.06846752131684752 1114 10-11 -0.05366511663745399 1114 12-13 0.06837449908907445 1114 14-15 0.02317999896515488 1114 16-17 2.3702006752345 1114 18-19 4.72017043494051 1114 20-21 0.8049512092665054 1114 22-23 0.11186570432081311 1114 24-25 -0.6230633740032232 1114 26-27 -0.6535015629634628 1114 28-29 -0.21490182380512834 1114 30-31 -0.3664543266613549 1114 32-33 -0.5435750278500677 1114 34-35 -0.6344691279932846 1114 36-37 -0.6874376142491343 1114 38-39 -0.8952990155131104 1114 40-41 -0.49830319087707764 1114 42-43 -0.42259657862453537 1114 44-45 1.6479870098576477 1114 46-47 0.06902332218453466 1114 48-49 -0.559614157484301 1114 50-51 -0.6047781248848771 1114 52-53 -0.6713234960879895 1114 54-55 -0.6508766779395998 1114 56-57 -0.8250019839766374 1114 58-59 -0.7539588204063925 1114 60-61 -0.5210130347623974 1114 62-63 -0.6542683484656493 1114 64-65 -0.49524380261978607 1114 66-67 -0.45883919077688873 1114 68-69 -0.5164469448664519 1114 70-71 -0.385059241749218 1114 72-73 -0.5314014666623592 1114 74-75 -0.4248644213526198 1114 76-77 -0.3678624036103102 1114 78-79 -0.5525297899590775 1114 80-81 -0.3733765093335357 1114 82-83 -0.2658487903218649 1114 84-85 -0.22166852322590813 1114 86-87 -0.26893679127445225 1114 88-89 -0.3859234264126421 1114 90-91 -0.39737582188822884 1114 92-93 -0.5676202742250602 1114 94-95 -0.5120823065084217 1114 96-97 -0.4783358631201473 1114 98-99 -0.5145829488901086 1114 100-101 -0.16993085186092216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 10.0 7 193.0 8 746.0 9 1415.0 10 2842.0 11 5120.0 12 9301.0 13 18004.0 14 32639.0 15 49072.0 16 68231.0 17 93866.0 18 116964.0 19 130500.0 20 139673.0 21 152584.0 22 170574.0 23 193714.0 24 219803.0 25 240497.0 26 253007.0 27 264000.0 28 282028.0 29 311168.0 30 349410.0 31 398103.0 32 459923.0 33 536910.0 34 643174.0 35 776234.0 36 841920.0 37 773166.0 38 413662.0 39 51467.0 40 80.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 16.634666517815184 42.24116018050913 21.74770249110552 19.376470810570172 2 17.6114875 43.9694125 19.2091 19.21 3 40.08429003160876 22.118320794370298 19.267019725132396 18.530369448888543 4 18.3854125 22.172449999999998 18.683275 40.7588625 5 18.33880295848978 44.050045036979874 20.507159309542246 17.1039926949881 6 40.23126877554008 22.384225289854772 19.488258986226157 17.89624694837899 7 18.0028625 22.25405 42.530925 17.2121625 8 39.9984125 22.710774999999998 19.8534 17.4374125 9 39.591474999999996 22.449175 20.332075 17.627275 10-11 17.221406249999998 33.6276875 31.65484375 17.4960625 12-13 17.124668749999998 22.557806250000002 31.60715 28.710375 14-15 17.06362361134132 44.766965842190224 20.66241115921953 17.50699938724893 16-17 28.524135681774133 22.28405487198418 20.67387201031286 28.51793743592883 18-19 17.038544390780924 34.03899358863766 20.681006084625082 28.241455935956335 20-21 28.316239269764953 33.367839585489975 20.74600129662508 17.56991984811999 22-23 28.261972282746534 22.124146515518316 32.044016505502064 17.569864696233086 24-25 22.29523125 29.87963125 23.1674 24.6577375 26-27 23.378109347324955 24.93231659950046 29.49911252354005 22.19046152963454 28-29 33.01749592294301 22.091608203276785 27.398304201795277 17.49259167198493 30-31 27.702531246828066 24.85290977883091 28.930791469642188 18.513767504698837 32-33 22.166799866573005 26.611516259524127 31.313586496698292 19.908097377204573 34-35 21.23929580354811 25.88138152487146 30.583349682615584 22.295972988964845 36-37 23.138625100265788 24.824577839938993 31.595207592212336 20.441589467582887 38-39 23.182521647815204 24.720053090006637 30.83265385408173 21.264771408096426 40-41 20.54389782923372 26.79756973177299 31.890589179418843 20.767943259574448 42-43 20.736405946723494 25.514160078276298 32.01121768579292 21.73821628920729 44-45 20.706491394324352 27.383881006044465 30.233192039750023 21.67643555988116 46-47 22.104016090362034 24.781751339405137 30.536137063083917 22.57809550714891 48-49 21.316355207127323 26.075381050323116 30.21479620523454 22.39346753731502 50-51 22.03530233902043 27.297559241452902 29.833087113090624 20.834051306436045 52-53 22.414377801797226 25.011684376460547 30.994010124251265 21.579927697490962 54-55 21.956610217457666 26.534194900646174 29.792234844176136 21.71696003772003 56-57 22.561923362524414 26.636391210263465 28.19764950941498 22.60403591779714 58-59 24.83290625 25.72255 27.810412499999998 21.63413125 60-61 22.891470858034218 26.677711712917795 27.601803082550408 22.829014346497576 62-63 23.040350460197136 27.510784224816128 27.725334345500567 21.72353096948617 64-65 21.64936875 26.616075 29.28539375 22.4491625 66-67 22.6876625 26.85635625 28.928462500000002 21.52751875 68-69 24.078319808384247 26.266265923422072 28.250636236880183 21.404778031313498 70-71 21.09368471651534 26.701421399879788 29.873660080159098 22.331233803445777 72-73 21.77529798884095 25.76395343779047 29.222356500604253 23.238392072764327 74-75 21.99202565231424 27.498730609449968 28.84141580734364 21.66782793089215 76-77 22.395005287306795 25.531194797582618 29.19450245096155 22.879297464149033 78-79 21.536131400365207 26.481526667480015 27.972541005738105 24.009800926416673 80-81 22.517901631139242 26.704379223763997 28.53439596334929 22.24332318174747 82-83 22.521229027618254 26.18108507592624 29.000861455105987 22.296824441349518 84-85 23.669879396231764 26.342392798612313 27.656839556380934 22.330888248774983 86-87 22.32255732233138 26.399419643797444 28.077437577614113 23.200585456257063 88-89 23.720279447552397 25.933511112533335 27.269273862988854 23.07693557692542 90-91 22.577880238997754 26.87257855211471 27.12479132009078 23.424749888796757 92-93 22.285888003268063 27.703602424020602 28.064334210287328 21.946175362424007 94-95 21.583380890651753 26.95855891418939 28.591778582875456 22.866281612283405 96-97 23.08028509762473 26.122830178738205 27.9714934875284 22.825391236108665 98-99 24.05078125 26.07659375 27.675499999999996 22.197125 100-101 21.731816875656023 27.395212089245817 28.40111951170043 22.471851523397728 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 98201.0 1 57734.0 2 13435.5 3 8171.5 4 6000.0 5 4811.5 6 4106.5 7 3692.0 8 3316.0 9 3209.5 10 3429.0 11 10515.0 12 51199.0 13 56109.0 14 24530.0 15 21370.0 16 21250.5 17 22076.5 18 23288.0 19 24493.0 20 25968.5 21 27860.0 22 30413.0 23 33700.5 24 37682.5 25 42440.5 26 48286.5 27 54957.5 28 61738.0 29 69018.5 30 77667.5 31 87957.0 32 99330.0 33 112555.0 34 127805.0 35 142615.5 36 156902.5 37 168376.5 38 174029.0 39 176141.5 40 179537.5 41 186673.5 42 194570.5 43 204156.5 44 217080.0 45 229015.5 46 243581.0 47 259518.0 48 270720.0 49 275096.5 50 279448.5 51 290869.0 52 313141.5 53 353763.5 54 360860.5 55 330047.0 56 299301.0 57 260078.0 58 216681.0 59 176967.0 60 143549.0 61 115266.0 62 91834.0 63 72215.0 64 57173.5 65 45924.5 66 36579.5 67 29112.5 68 22925.0 69 17732.5 70 13824.0 71 11155.5 72 9439.5 73 7524.5 74 5669.0 75 4215.0 76 2876.0 77 1823.0 78 1132.5 79 684.0 80 360.5 81 197.0 82 130.5 83 91.0 84 58.5 85 42.5 86 29.5 87 14.0 88 10.0 89 7.5 90 5.0 91 3.5 92 2.5 93 0.5 94 1.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.12416250000000001 2 0.0 3 3.7500000000000003E-5 4 0.0 5 0.0959875 6 0.0070375 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.75E-4 16-17 2.19006875 18-19 5.12254375 20-21 6.2499999999999995E-6 22-23 1.2499999999999999E-5 24-25 0.0 26-27 8.6875E-4 28-29 6.3125E-4 30-31 0.0018499999999999999 32-33 0.00149375 34-35 0.00198125 36-37 1.625E-4 38-39 1.2499999999999999E-5 40-41 7.5E-4 42-43 0.00165625 44-45 2.39 46-47 0.0022500000000000003 48-49 0.001725 50-51 6.0625E-4 52-53 1.2499999999999999E-5 54-55 7.500000000000001E-5 56-57 1.3125E-4 58-59 0.0 60-61 4.1875E-4 62-63 5.625E-5 64-65 0.0 66-67 0.0 68-69 3.9375E-4 70-71 4.3125E-4 72-73 4.5E-4 74-75 0.001225 76-77 0.00225625 78-79 0.0018 80-81 0.00125625 82-83 0.00204375 84-85 0.00260625 86-87 0.00106875 88-89 1.8750000000000002E-5 90-91 1.0625E-4 92-93 0.00188125 94-95 5.625E-5 96-97 4.375E-5 98-99 0.0 100-101 0.036775 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8000000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.2559967070725 #Duplication Level Percentage of deduplicated Percentage of total 1 87.6937733928795 56.348508143636266 2 7.001303467235759 8.997514650718323 3 1.9773454548872675 3.8116890911394306 4 0.9530623974778607 2.4495989709588812 5 0.6210597695605928 1.9953407253890327 6 0.40439682033571733 1.5590952453505462 7 0.28204096521876426 1.2685976332649527 8 0.19629722057619042 1.009061884716094 9 0.14317093949253826 0.8279632274922911 >10 0.6628878065997836 7.317157824322409 >50 0.03677387200148166 1.567153149413711 >100 0.022512838262702384 3.3084080954188146 >500 0.004153566185031884 1.7841187439582653 >1k 0.0011400566704854393 1.0684180487116826 >5k 2.0358154043235376E-5 0.10692683058688227 >10k+ 6.107446212970613E-5 6.5804477349224175 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 344847 4.3105875000000005 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 120587 1.5073375 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA 37607 0.4700875 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA 8174 0.10217500000000002 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 1.25E-5 0.0 1.25E-5 0.0 2 0.0 1.25E-5 0.0 2.5E-5 0.0 3 0.0 1.25E-5 0.0 3.75E-5 0.0 4 0.0 1.25E-5 0.0 3.75E-5 0.0 5 0.0 1.25E-5 0.0 5.0E-5 0.0 6 0.0 1.25E-5 0.0 5.0E-5 0.0 7 0.0 2.5E-5 0.0 6.25E-5 0.0 8 0.0 2.5E-5 0.0 7.5E-5 1.25E-5 9 0.0 2.5E-5 0.0 7.5E-5 1.25E-5 10-11 0.0 3.75E-5 0.0 8.75E-5 1.25E-5 12-13 2.5E-5 4.375E-5 0.0 8.75E-5 1.25E-5 14-15 3.125E-5 5.0E-5 0.0 1.0625000000000001E-4 1.25E-5 16-17 3.75E-5 5.0E-5 0.0 1.125E-4 1.25E-5 18-19 6.25E-5 6.25E-5 0.0 1.125E-4 2.5E-5 20-21 9.375E-5 6.25E-5 0.0 1.125E-4 2.5E-5 22-23 1.125E-4 7.5E-5 0.0 1.125E-4 3.75E-5 24-25 1.5625E-4 8.125E-5 0.0 1.125E-4 3.75E-5 26-27 2.25E-4 8.75E-5 0.0 1.1875E-4 3.75E-5 28-29 2.6250000000000004E-4 8.75E-5 0.0 1.3125000000000002E-4 3.75E-5 30-31 3.0625E-4 9.375E-5 0.0 1.375E-4 4.375E-5 32-33 3.875E-4 1.0E-4 0.0 1.4375E-4 7.500000000000001E-5 34-35 4.75E-4 1.0E-4 0.0 1.875E-4 9.375E-5 36-37 5.499999999999999E-4 1.0E-4 0.0 2.0E-4 1.0625000000000001E-4 38-39 6.000000000000001E-4 1.125E-4 0.0 2.0625E-4 1.3125000000000002E-4 40-41 6.75E-4 1.125E-4 0.0 2.25E-4 1.5E-4 42-43 7.4375E-4 1.125E-4 0.0 2.25E-4 1.5E-4 44-45 8.0625E-4 1.125E-4 0.0 2.25E-4 1.625E-4 46-47 9.125E-4 1.25E-4 0.0 2.25E-4 1.625E-4 48-49 0.00105625 1.375E-4 0.0 2.3125E-4 1.6875E-4 50-51 0.00114375 1.5E-4 0.0 2.375E-4 1.875E-4 52-53 0.0012812499999999998 1.625E-4 0.0 2.4375E-4 1.875E-4 54-55 0.0013874999999999998 1.6875E-4 0.0 2.625E-4 2.0625E-4 56-57 0.0015375 1.8125E-4 0.0 2.625E-4 2.25E-4 58-59 0.0016749999999999998 2.0E-4 0.0 2.625E-4 2.25E-4 60-61 0.00183125 2.0625E-4 0.0 2.6875E-4 2.25E-4 62-63 0.00199375 2.25E-4 0.0 2.875E-4 2.25E-4 64-65 0.00210625 2.3125E-4 0.0 2.875E-4 2.3125E-4 66-67 0.0023625 2.375E-4 0.0 2.875E-4 2.4375E-4 68-69 0.0027124999999999996 2.5624999999999997E-4 0.0 2.875E-4 2.5624999999999997E-4 70-71 0.0031625 2.625E-4 0.0 2.875E-4 2.625E-4 72-73 0.0036812499999999996 2.625E-4 0.0 2.875E-4 2.8125000000000003E-4 74-75 0.0046375 2.8125E-4 0.0 2.875E-4 3.0000000000000003E-4 76-77 0.0058625 3.0E-4 0.0 2.875E-4 3.125E-4 78-79 0.007525 3.0625E-4 0.0 2.9374999999999996E-4 3.1875E-4 80-81 0.009787500000000001 3.125E-4 0.0 3.0E-4 3.25E-4 82-83 0.01326875 3.125E-4 0.0 3.0625E-4 3.25E-4 84-85 0.01803125 3.125E-4 0.0 3.125E-4 3.5E-4 86-87 0.024743750000000002 3.25E-4 0.0 3.125E-4 3.5E-4 88-89 0.033737500000000004 3.25E-4 0.0 3.375E-4 3.625E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 236075 0.0 70.41046 9 TGGTATC 236895 0.0 70.13512 7 GTGGTAT 235720 0.0 70.10205 6 GGTATCA 237340 0.0 70.02728 8 AGTGGTA 240035 0.0 68.73817 5 CAGTGGT 241585 0.0 68.11288 4 AAGCAGT 244830 0.0 66.852745 1 AGCAGTG 250025 0.0 65.614944 2 GCAGTGG 252945 0.0 65.09462 3 AGAGTAC 63960 0.0 43.511745 18-19 GAGTACT 54265 0.0 42.964783 20-21 AGTACTT 53220 0.0 42.647232 20-21 GTACTTT 52250 0.0 42.64217 22-23 TAACGCC 10030 0.0 41.35575 54-55 CTTAACG 10035 0.0 41.311817 52-53 TACTTTT 56360 0.0 39.756817 22-23 ACGCAGA 215295 0.0 39.55331 14-15 AGACTCG 10190 0.0 39.44318 66-67 CAACGCA 216555 0.0 39.397877 12-13 AACGCCC 10610 0.0 39.359768 54-55 >>END_MODULE