##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR799685_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5550218 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.560958686667803 31.0 28.0 31.0 16.0 34.0 2 28.84038879193574 31.0 28.0 33.0 20.0 34.0 3 28.533006631451233 31.0 27.0 34.0 16.0 34.0 4 31.257704832494866 35.0 30.0 37.0 16.0 37.0 5 32.03777257037471 35.0 32.0 37.0 19.0 37.0 6 31.59930204543317 35.0 31.0 37.0 17.0 37.0 7 31.485281839380004 35.0 31.0 37.0 17.0 37.0 8 31.343553532491878 35.0 30.0 37.0 17.0 37.0 9 32.37899159996959 37.0 30.0 39.0 16.0 39.0 10-11 32.66654453933161 37.0 30.5 39.0 16.5 39.0 12-13 32.42642270988274 37.0 30.0 39.0 16.5 39.0 14-15 33.99789710962705 38.0 31.5 40.0 17.0 41.0 16-17 33.39422883929964 38.0 30.5 40.0 16.5 41.0 18-19 33.39762789497638 38.0 30.5 40.0 17.0 41.0 20-21 33.543659185999545 38.0 30.5 40.0 17.5 41.0 22-23 29.51631530509252 35.0 20.0 39.5 9.5 40.5 24-25 29.600207145016647 33.0 21.5 39.0 13.0 40.0 26-27 30.779383170174576 34.5 25.5 38.5 10.5 40.0 28-29 31.45257816900165 36.0 27.0 39.5 12.0 41.0 30-31 30.636520583515818 34.5 25.0 39.5 9.5 41.0 32-33 30.130234343948292 34.0 24.5 39.0 9.0 40.0 34-35 30.3764554833702 34.0 25.0 39.0 9.0 40.5 36-37 30.464871469913433 34.0 25.0 39.0 9.0 41.0 38-39 30.221911914090583 34.0 25.0 39.0 9.0 40.0 40-41 29.869788087603048 33.0 24.0 38.5 9.0 40.0 42-43 30.10309613063847 34.0 24.0 39.0 9.0 40.0 44-45 29.991704830332793 34.0 24.0 38.0 9.0 40.0 46-47 29.65217267141579 33.0 24.0 38.0 9.0 40.0 48-49 29.476116793970974 33.0 24.0 38.0 9.0 40.0 50-51 29.161962286886748 33.0 23.0 38.0 8.0 40.0 52-53 28.911927153131643 32.5 22.5 38.0 8.0 40.0 54-55 28.721036993501876 32.0 23.0 37.5 8.0 40.0 56-57 28.624619609536058 32.0 22.5 37.0 8.0 40.0 58-59 28.571599259704755 32.0 23.0 37.0 8.0 40.0 60-61 28.043883321339813 31.5 22.0 36.0 8.0 39.5 62-63 27.789443585819512 31.0 22.0 35.5 8.0 39.0 64-65 27.522653614686845 31.0 21.0 35.0 8.0 39.0 66-67 27.27687912438755 31.0 20.5 35.0 8.0 39.0 68-69 27.14370669764683 31.0 20.5 35.0 8.0 38.0 70-71 26.590389062195396 30.0 20.0 35.0 7.0 37.5 72-73 26.552618293551713 30.0 20.0 35.0 7.0 37.0 74-75 26.50122905082287 30.0 20.0 34.5 7.0 36.5 76-77 26.16361897856985 30.0 20.0 34.0 7.0 36.0 78-79 25.831802552620456 29.0 19.5 34.0 7.0 36.0 80-81 25.63010083567889 29.0 19.0 34.0 7.0 35.0 82-83 25.509536021828332 29.0 18.5 34.0 7.0 35.0 84-85 25.262484014141428 29.0 18.0 34.0 7.0 35.0 86-87 25.165575478296528 29.0 18.0 34.0 7.0 35.0 88-89 25.061592535644543 29.0 18.0 34.0 7.0 35.0 90-91 24.97210875320573 29.0 18.0 34.0 7.0 35.0 92-93 24.778935800359555 29.0 18.0 34.0 7.0 35.0 94-95 24.670680142653858 29.0 17.5 34.0 7.0 35.0 96-97 24.527129925347076 28.5 17.5 34.0 7.0 35.0 98-99 24.399228732997514 28.0 17.0 34.0 7.0 35.0 100-101 23.09137037500149 26.0 12.5 32.5 7.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2105 1 -0.2544109558090817 2105 2 -0.20867505892357485 2105 3 -0.26311953347429196 2105 4 -0.39255952835756247 2105 5 -0.3387920977809351 2105 6 -0.30648664586457386 2105 7 -0.24554302072483836 2105 8 -0.16810216139716871 2105 9 -0.0881893065146997 2105 10-11 -0.005982326366300583 2105 12-13 -0.022464707288669672 2105 14-15 -0.22414922393857495 2105 16-17 -0.32456522450593184 2105 18-19 -0.2900158936941395 2105 20-21 -0.2369186900060427 2105 22-23 3.245810513102196 2105 24-25 2.4572053107997682 2105 26-27 0.7298904526203742 2105 28-29 -0.04746558250391075 2105 30-31 0.02695883539425381 2105 32-33 0.04762904993836514 2105 34-35 0.21304232983857574 2105 36-37 0.08618620469483673 2105 38-39 -0.01425032494798728 2105 40-41 0.1049877332830782 2105 42-43 -0.03628573825210779 2105 44-45 -0.0600407797079221 2105 46-47 0.04670828392579196 2105 48-49 0.11754849122320365 2105 50-51 0.03264606432354711 2105 52-53 0.13701968054217772 2105 54-55 0.11645812856320603 2105 56-57 -0.1764539250227557 2105 58-59 -0.13922392098364256 2105 60-61 0.11929709537700361 2105 62-63 -0.05027630160507712 2105 64-65 -0.3226601170020018 2105 66-67 -0.27228928117465045 2105 68-69 -0.054638565926133253 2105 70-71 -0.06229975783316988 2105 72-73 -0.14494473608643688 2105 74-75 -0.09705160870815988 2105 76-77 -0.2203954305742819 2105 78-79 -0.14501490739634448 2105 80-81 -0.07833470633955741 2105 82-83 -0.010364325033147992 2105 84-85 -0.22652426954311622 2105 86-87 -0.17192137144343533 2105 88-89 -0.022294850345090822 2105 90-91 -0.23487587751408512 2105 92-93 -0.012705085416381934 2105 94-95 -0.21557835238897027 2105 96-97 -0.058480777453272736 2105 98-99 -0.16320657243787196 2105 100-101 -0.1759299344625198 2106 1 -0.34109097740991245 2106 2 -0.23978446602233205 2106 3 -1.2605854529770681 2106 4 -0.5972722481844137 2106 5 -0.43618886119807954 2106 6 -0.3369124218180666 2106 7 -0.40233049316221425 2106 8 -0.28224872587112415 2106 9 -0.3015223476744566 2106 10-11 -0.5078207810922493 2106 12-13 -2.2925353312463663 2106 14-15 -1.4519520115747113 2106 16-17 -0.7736180818084577 2106 18-19 -2.644173711353613 2106 20-21 -1.5674281957033713 2106 22-23 0.9446081062522858 2106 24-25 1.1506742882192533 2106 26-27 0.004869605234944174 2106 28-29 -0.40654642493239734 2106 30-31 -0.7702324634563915 2106 32-33 -0.4954291934640409 2106 34-35 -0.11723497540847205 2106 36-37 -0.2176508924245617 2106 38-39 -1.2955986865095923 2106 40-41 -1.7927818316154251 2106 42-43 -0.6489572138757786 2106 44-45 -0.8170674667627011 2106 46-47 -0.5791606573121975 2106 48-49 -0.5425697225075794 2106 50-51 -2.446074243411278 2106 52-53 -2.5759604259390834 2106 54-55 -1.799172522606657 2106 56-57 -1.062306022253722 2106 58-59 -0.49230291021114425 2106 60-61 -1.9858998271146469 2106 62-63 -1.3914447522816218 2106 64-65 -0.780665993293109 2106 66-67 -0.7457672994653173 2106 68-69 -0.9103016403586537 2106 70-71 -2.0492409871174857 2106 72-73 -1.8768456083946745 2106 74-75 -1.283854150598298 2106 76-77 -2.268147475063909 2106 78-79 -1.5538803018271778 2106 80-81 -2.3352169450775904 2106 82-83 -0.8778373532592134 2106 84-85 -0.4807331075137533 2106 86-87 -0.32431241946506617 2106 88-89 -1.2235015872674957 2106 90-91 -0.742809528396073 2106 92-93 -0.7668635809785975 2106 94-95 -0.46160512939718856 2106 96-97 -0.4059078463749195 2106 98-99 -0.4452201416993731 2106 100-101 -0.32612501536008054 2107 1 -0.03902165541520475 2107 2 -0.053495045324115864 2107 3 0.04099732526169575 2107 4 -0.030832941362284316 2107 5 -0.16240761364746348 2107 6 -0.15376920984973808 2107 7 -0.03938023016447545 2107 8 0.014300986923355907 2107 9 0.026044586420702842 2107 10-11 0.07327278854430119 2107 12-13 0.26795886091829146 2107 14-15 -0.16732549218802717 2107 16-17 -0.06975835896327709 2107 18-19 0.07820572286769334 2107 20-21 -0.010473549661107029 2107 22-23 3.5110368337186166 2107 24-25 2.780454756884783 2107 26-27 0.9144314970890086 2107 28-29 0.363438091894313 2107 30-31 0.449947964418385 2107 32-33 0.44819815299585386 2107 34-35 0.4679889780043247 2107 36-37 0.3652455174232472 2107 38-39 0.4650564518789011 2107 40-41 0.5408334946777735 2107 42-43 0.4155525954223265 2107 44-45 0.4191288193567537 2107 46-47 0.5732831440268207 2107 48-49 0.5499792105489334 2107 50-51 0.5012817449390781 2107 52-53 0.3194083805677188 2107 54-55 0.24863623658537293 2107 56-57 0.18665669980649824 2107 58-59 0.1568614223162932 2107 60-61 0.3177361492850501 2107 62-63 0.25704303097538883 2107 64-65 0.26378924159673645 2107 66-67 0.2674326590729912 2107 68-69 0.17034147331441574 2107 70-71 0.16331718725684752 2107 72-73 0.1349171284887234 2107 74-75 0.10933199301421581 2107 76-77 0.21416231394684715 2107 78-79 0.3032477511218872 2107 80-81 0.3505385255474529 2107 82-83 0.16875576083603505 2107 84-85 0.10809101809129373 2107 86-87 0.20377095137398626 2107 88-89 0.2778608717111162 2107 90-91 0.17976660579402548 2107 92-93 0.2028289989334553 2107 94-95 0.18816974603364756 2107 96-97 0.21441535340678541 2107 98-99 0.19026387907389264 2107 100-101 0.20497836176929596 2108 1 0.2959669824921001 2108 2 0.20762439058223237 2108 3 0.23153935163001194 2108 4 0.2063209030613642 2108 5 0.13899907362706188 2108 6 0.07066194058566566 2108 7 0.004955034782273771 2108 8 0.1674593163839333 2108 9 0.1687633401578097 2108 10-11 0.02188982842584153 2108 12-13 0.34683990379894425 2108 14-15 0.27010343163703254 2108 16-17 0.14759511906422063 2108 18-19 0.37993091988443695 2108 20-21 0.1951310251557814 2108 22-23 3.499398255353608 2108 24-25 2.7367707637914087 2108 26-27 0.769624441756779 2108 28-29 0.26195148332758933 2108 30-31 0.5423454068084332 2108 32-33 0.35065861471242243 2108 34-35 0.41541926141472274 2108 36-37 0.2777158007678011 2108 38-39 0.33855595842478436 2108 40-41 0.5307989170739447 2108 42-43 0.3696161589687854 2108 44-45 0.36732104669279764 2108 46-47 0.3367211796199463 2108 48-49 0.182736403950706 2108 50-51 0.41314159736028344 2108 52-53 0.3461236825887575 2108 54-55 0.3122379265401669 2108 56-57 0.19953496098996482 2108 58-59 0.1474856044447641 2108 60-61 0.24964898836813632 2108 62-63 0.1478100653726777 2108 64-65 0.14020627204657288 2108 66-67 0.1571998414017024 2108 68-69 0.21498693706862682 2108 70-71 0.26001812811124836 2108 72-73 0.3210071076773744 2108 74-75 0.21076210651502691 2108 76-77 0.23660799139463862 2108 78-79 0.25337144933854105 2108 80-81 0.28539780480030075 2108 82-83 0.20395447602532002 2108 84-85 0.1996683400124617 2108 86-87 0.07384675790149586 2108 88-89 0.1927580380238041 2108 90-91 0.13467754067316662 2108 92-93 0.04795283623368718 2108 94-95 0.0735086115538266 2108 96-97 0.1517497929957301 2108 98-99 0.19837787842648424 2108 100-101 0.05211751233170858 2109 1 0.29085293222469133 2109 2 0.23466956875477862 2109 3 0.2922943501671291 2109 4 0.18078087831254308 2109 5 0.25876972285546884 2109 6 0.2234972722227404 2109 7 0.20329262420495908 2109 8 0.2616630179970123 2109 9 0.20859609097185938 2109 10-11 0.2227713513685785 2109 12-13 0.3589928105427518 2109 14-15 0.32424801586205376 2109 16-17 0.27292739272470357 2109 18-19 0.5085508034519108 2109 20-21 0.24049119208938663 2109 22-23 3.682684933068682 2109 24-25 3.004062874698729 2109 26-27 0.5766721591106645 2109 28-29 0.3651816861388717 2109 30-31 0.489155043429367 2109 32-33 0.331280271342969 2109 34-35 0.4228219340812096 2109 36-37 0.3307760431224196 2109 38-39 0.42558632482946734 2109 40-41 0.4632569276955074 2109 42-43 0.3691840740814847 2109 44-45 0.43560555369011666 2109 46-47 0.5671156314187336 2109 48-49 0.4186685343628014 2109 50-51 0.49130695045651507 2109 52-53 0.41169109420440364 2109 54-55 0.18390177472952018 2109 56-57 0.05730201813599223 2109 58-59 0.12210677173002082 2109 60-61 0.2767426005802882 2109 62-63 0.33779884184106734 2109 64-65 0.2938719596199739 2109 66-67 0.25031215608109747 2109 68-69 0.2536457941973822 2109 70-71 0.32116302744721636 2109 72-73 0.2711880382159606 2109 74-75 0.30617751586698105 2109 76-77 0.27177774857380754 2109 78-79 0.1869141901410849 2109 80-81 0.15215579266316226 2109 82-83 0.1431314368464136 2109 84-85 0.18081874004508336 2109 86-87 0.2029508031188918 2109 88-89 0.3920773320520041 2109 90-91 0.3024569739552092 2109 92-93 0.23903751759847935 2109 94-95 0.20419482512557963 2109 96-97 0.17072934756873792 2109 98-99 0.16948365158895484 2109 100-101 0.15874614765524342 2110 1 -0.30064003448908494 2110 2 -0.320006760413051 2110 3 -0.2712826200837384 2110 4 -0.42871258581879346 2110 5 -0.3902023403780106 2110 6 -0.2756702283913057 2110 7 -0.24728411091365743 2110 8 -0.26407365480028133 2110 9 -0.06341908768521165 2110 10-11 -0.548070084714773 2110 12-13 -0.09230261924155769 2110 14-15 -0.1966864232218839 2110 16-17 -0.3118904952800534 2110 18-19 -0.1571153458798875 2110 20-21 -0.13995755083958983 2110 22-23 -12.03017915939921 2110 24-25 -10.323346643899708 2110 26-27 -2.5813794653244386 2110 28-29 -0.7580564208689218 2110 30-31 -0.7289432682967281 2110 32-33 -0.5024729802159484 2110 34-35 -0.06948674246614317 2110 36-37 -0.13068541819959378 2110 38-39 0.06817029076515269 2110 40-41 -0.4452611935088733 2110 42-43 -0.3739497943375163 2110 44-45 -1.013264116956794 2110 46-47 -0.14217893661079373 2110 48-49 -0.01884623422493803 2110 50-51 0.03945922709929661 2110 52-53 0.07008372119855366 2110 54-55 -0.0734264336511039 2110 56-57 -0.139662594928339 2110 58-59 -0.18940401468035617 2110 60-61 -0.14959139882178718 2110 62-63 -0.19344204830384015 2110 64-65 -0.19597414792804457 2110 66-67 -0.09912446496075589 2110 68-69 -0.17752778702844907 2110 70-71 -0.08662396238601744 2110 72-73 -0.1658538379443968 2110 74-75 -0.05724620048454199 2110 76-77 -0.05254896783275953 2110 78-79 -0.0652407097093679 2110 80-81 -0.007002008333792276 2110 82-83 -0.04065468839516129 2110 84-85 -0.11236333137255983 2110 86-87 -0.08639401274681902 2110 88-89 -0.01121176874100982 2110 90-91 -0.07987253572020592 2110 92-93 -0.14505224816060647 2110 94-95 -0.060460920408178964 2110 96-97 -0.09164865715260007 2110 98-99 -0.06341274389159324 2110 100-101 -0.14851597141397832 2111 1 0.3114978157596404 2111 2 0.2674162612712685 2111 3 0.3232714131753234 2111 4 0.3563647944756596 2111 5 0.30274747285253767 2111 6 0.17640262052459477 2111 7 0.39842631510387605 2111 8 0.16210383655385385 2111 9 0.37390438715447516 2111 10-11 0.43946378650299067 2111 12-13 0.5247703833442117 2111 14-15 0.3542657476809623 2111 16-17 0.3396376705680595 2111 18-19 0.4258461418826016 2111 20-21 0.39134419143231725 2111 22-23 1.7686285798648456 2111 24-25 1.9759329552592284 2111 26-27 0.3623953785182259 2111 28-29 0.19146519446904264 2111 30-31 -0.33095483606241416 2111 32-33 -0.2752955679295539 2111 34-35 0.3096689293135775 2111 36-37 -1.0043280922374294 2111 38-39 0.0361212868012899 2111 40-41 -0.44096270579841956 2111 42-43 -0.2963034014596708 2111 44-45 0.36482923341672446 2111 46-47 -0.6624219938922344 2111 48-49 0.031191214231810704 2111 50-51 0.23524295787622762 2111 52-53 0.16325368950053232 2111 54-55 0.25049102779622245 2111 56-57 0.21255976907813334 2111 58-59 0.22718195949569164 2111 60-61 0.36571020901451945 2111 62-63 0.25215929983476215 2111 64-65 0.2356067802432129 2111 66-67 0.16594401853420848 2111 68-69 0.2458208580308998 2111 70-71 0.37907589412164455 2111 72-73 0.2677504539426607 2111 74-75 0.3191741768229974 2111 76-77 0.32836040841963765 2111 78-79 0.42131264413332303 2111 80-81 0.3534930847523512 2111 82-83 0.30781716764701983 2111 84-85 0.1338307782176713 2111 86-87 0.08640282176479985 2111 88-89 0.27002978981411374 2111 90-91 0.2258214524372626 2111 92-93 0.35042939071263035 2111 94-95 0.3389972991770982 2111 96-97 0.3252658465656708 2111 98-99 0.3183029540677573 2111 100-101 0.15400904634707757 2112 1 0.20246895601673387 2112 2 0.22113727173553954 2112 3 0.06145438486266386 2112 4 0.18720464728752972 2112 5 0.13632531833863482 2112 6 0.19393013317201024 2112 7 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1.7993898388736689 2215 26-27 0.06159922297803888 2215 28-29 -0.5594635692838992 2215 30-31 -0.8019679943378968 2215 32-33 -0.5457541402657995 2215 34-35 -1.026559745305537 2215 36-37 -0.7259834712084228 2215 38-39 -0.7660800591339729 2215 40-41 -0.49359630632087814 2215 42-43 -0.5074517085442061 2215 44-45 -1.0647925268983656 2215 46-47 -0.5063794357591931 2215 48-49 -0.5371881002516474 2215 50-51 -0.37995250151267257 2215 52-53 -0.43390690940342225 2215 54-55 -0.5425885568486812 2215 56-57 -0.6051684176101801 2215 58-59 -0.4814599807019704 2215 60-61 -0.41571849115593906 2215 62-63 -0.4268565932435706 2215 64-65 -0.46536535270204027 2215 66-67 -0.427956317080632 2215 68-69 -0.44781577154831 2215 70-71 -0.3062336510959831 2215 72-73 -0.3246943571237111 2215 74-75 -0.3518043655813017 2215 76-77 -0.38672428514913904 2215 78-79 -0.37396829265642495 2215 80-81 -0.3424577725891389 2215 82-83 -0.36148625011431434 2215 84-85 -0.44616790893026703 2215 86-87 -0.43399843063659915 2215 88-89 -0.4140969612418637 2215 90-91 -0.38760194340468246 2215 92-93 -0.4523884682424075 2215 94-95 -0.4751996653929247 2215 96-97 -0.39351601293531147 2215 98-99 -0.5190776841308846 2215 100-101 -0.33777016457660025 2216 1 -0.906931191736625 2216 2 -0.30489608404376156 2216 3 -0.15257668014690395 2216 4 -0.09755009753010668 2216 5 -0.10354532912238668 2216 6 0.03454876288580877 2216 7 0.050979863378675105 2216 8 0.08412974028528097 2216 9 -0.15217673154101874 2216 10-11 -0.23852777261441815 2216 12-13 -0.004889886910845576 2216 14-15 -0.17361923332912 2216 16-17 -0.07030224857636114 2216 18-19 -0.004737349719881934 2216 20-21 -0.15686158115909876 2216 22-23 3.2048505661358497 2216 24-25 2.0139300366939885 2216 26-27 0.22371425816894686 2216 28-29 -0.16592428764907297 2216 30-31 -0.30296418117018575 2216 32-33 -0.4990001270448232 2216 34-35 -0.5682454271172368 2216 36-37 -0.6649108143561833 2216 38-39 -0.5582247507768834 2216 40-41 -0.4932646444929034 2216 42-43 -0.5900962242555678 2216 44-45 -0.5413924983922307 2216 46-47 -0.7241384485656894 2216 48-49 -0.5886312265789186 2216 50-51 -0.6189756757122176 2216 52-53 -0.6422279094575138 2216 54-55 -0.7764378391882083 2216 56-57 -0.7246247634124643 2216 58-59 -0.691233724807649 2216 60-61 -0.6768475024141516 2216 62-63 -0.653671432661401 2216 64-65 -0.6830877861427389 2216 66-67 -0.6517831763712927 2216 68-69 -0.7406074544314869 2216 70-71 -0.699744388862765 2216 72-73 -0.6077113366837565 2216 74-75 -0.7202921961727498 2216 76-77 -0.7390927118175625 2216 78-79 -0.8182534524074683 2216 80-81 -0.6854531802979444 2216 82-83 -0.6206027532784866 2216 84-85 -0.61645893448976 2216 86-87 -0.7282605072401473 2216 88-89 -0.8060009361740086 2216 90-91 -0.762347984281611 2216 92-93 -0.7220822262014224 2216 94-95 -0.6262476459066875 2216 96-97 -0.695596074709389 2216 98-99 -0.70633564289124 2216 100-101 -0.5682183315450438 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 231.0 3 239.0 4 226.0 5 282.0 6 274.0 7 514.0 8 1729.0 9 7094.0 10 25024.0 11 58577.0 12 91743.0 13 107683.0 14 110136.0 15 111006.0 16 111795.0 17 114920.0 18 117733.0 19 121591.0 20 127013.0 21 133303.0 22 141879.0 23 152940.0 24 165811.0 25 174137.0 26 176677.0 27 178393.0 28 189778.0 29 204485.0 30 220306.0 31 244129.0 32 276099.0 33 315545.0 34 372304.0 35 436933.0 36 454172.0 37 390095.0 38 190600.0 39 24795.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.134548396525165 52.58107167823436 19.336125591389475 15.948254333851 2 16.05672457818776 53.825620306815125 14.792251953860596 15.325403161136512 3 48.43483436684176 20.622372041048646 15.412001071862157 15.530792520247436 4 15.353836977020578 20.468004242133176 15.748135506780836 48.43002327406541 5 15.287838167546077 53.666104337979604 16.242907973306625 14.803149521167693 6 47.808008089605494 21.140400854943874 15.634222116032937 15.417368939417692 7 15.576648597214612 21.271030967965167 48.563100112453505 14.589220322366714 8 46.89284645353528 22.159573028455938 15.865115971484055 15.08246454652473 9 46.62260287328998 21.689331152852006 16.343355160975104 15.344710812882912 10-11 14.405359364304848 37.8759591482991 32.52106380350487 15.197617683891174 12-13 14.309136127305658 21.945168818764248 32.25243969471151 31.493255359218576 14-15 14.51832131783054 53.7462716140065 16.289592434013098 15.445814634149857 16-17 30.832467800324935 21.03682669874162 16.241270746323877 31.889434754609564 18-19 14.80752049653715 37.06888256436556 16.489247449397908 31.63434948969938 20-21 31.226700519688332 36.848065043932046 16.543604085427823 15.3816303509518 22-23 32.78480041648707 19.436445671091125 30.394169420420415 17.384584492001387 24-25 24.685541580828087 29.614803258293726 21.63757059337342 24.062084567504762 26-27 26.96980149815806 25.43600433978572 22.484403838747234 25.10979032330899 28-29 46.430071989051044 19.554862563981104 18.261027947916403 15.75403749905145 30-31 35.90002053871268 26.031020395509458 20.660674241146836 17.408284824631025 32-33 25.271687530027908 29.988688642237822 24.45518972862745 20.284434099106814 34-35 22.21494780170216 28.189703229476237 23.818475402015117 25.776873566806486 36-37 26.91909067083592 25.014690206084666 26.768511552269914 21.297707570809497 38-39 28.421977586961994 24.37088221289024 24.373847591954465 22.833292608193297 40-41 21.577550995202156 29.210597038351665 27.22528636837062 21.98656559807556 42-43 21.724104015137645 25.880113559375665 27.565302455828327 24.83047996965836 44-45 21.47562433883659 31.945631239500827 23.238110777521413 23.340633644141175 46-47 25.398675060009346 24.562205465436076 23.72251649501772 26.316602979536853 48-49 22.70509855464726 27.94319473740402 23.796967110939264 25.55473959700946 50-51 25.26241602998128 29.452100559065236 23.364618540161743 21.920864870791736 52-53 26.093400903404024 24.542824438689518 26.730721406319898 22.633053251586563 54-55 24.34717918377628 27.129257426657155 25.2480163513301 23.275547038236464 56-57 25.028874864412504 26.3439338812468 23.826115999090135 24.801075255250556 58-59 29.83191910197022 24.31199585757671 23.668054534696175 22.188030505756895 60-61 26.15795690478412 26.658753003113638 23.504932745126407 23.67835734697584 62-63 25.754604065095027 28.064699844937145 23.663923755445275 22.51677233452255 64-65 22.79357202632139 26.482119294104546 26.041425784675248 24.68288289489882 66-67 25.89527921565037 25.987385574521976 26.13451241489174 21.982822794935917 68-69 28.997152276299943 25.320741790613404 23.86184101610718 21.82026491697947 70-71 22.455952682691812 27.394389922320876 27.221427259135574 22.928230135851738 72-73 23.105608128181153 25.255080180800455 26.08935805530754 25.549953635710853 74-75 23.26782756839889 29.90915892731678 24.443753985769323 22.37925951851501 76-77 25.3482463669192 24.429351192930053 25.021655436836994 25.200747003313754 78-79 23.121487389360055 26.596305350607118 23.802039439544735 26.480167820488088 80-81 25.887950078354038 26.612705763268522 24.95793015933324 22.541413999044195 82-83 26.024329325182826 25.18872176697676 26.292658724616253 22.49429018322416 84-85 27.116889070613936 25.53651487118976 24.05778436040522 23.288811697791086 86-87 24.755765368232964 25.726324212248812 24.583937435854 24.933972983664226 88-89 28.346798771421454 24.367741991296427 23.52908682414032 23.756372413141797 90-91 25.822813299656733 26.521594071053688 23.53097418726878 24.1246184420208 92-93 24.691662248798707 28.26622498798977 25.19630830819864 21.84580445501289 94-95 22.7482078480067 26.95270823388946 25.96022461386046 24.33885930424338 96-97 26.056328446392552 24.900456796280753 25.45163091004686 23.591583847279836 98-99 28.400263605222253 24.926634896223693 24.346022135737584 22.32707936281647 100-101 23.55924913916622 28.22116222609281 25.643122098972587 22.57646653576838 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23700.0 1 17529.0 2 8814.0 3 5527.5 4 4504.5 5 4139.0 6 4005.0 7 4022.0 8 4162.0 9 4289.5 10 4550.0 11 5749.0 12 25553.5 13 26935.5 14 8303.0 15 6899.0 16 6706.0 17 7023.0 18 7665.5 19 8245.5 20 9019.0 21 10386.5 22 12071.0 23 14120.0 24 16430.0 25 18933.5 26 21896.0 27 24947.5 28 28428.5 29 32887.5 30 37833.5 31 43393.0 32 49246.5 33 55661.5 34 63655.5 35 72381.5 36 80529.5 37 87214.5 38 91657.5 39 94789.0 40 99533.0 41 104949.0 42 110237.0 43 116892.0 44 125177.5 45 133609.5 46 142677.0 47 153471.5 48 163437.5 49 171696.5 50 184548.0 51 204169.0 52 233356.5 53 281930.5 54 299252.5 55 282920.0 56 274516.0 57 255665.0 58 223589.0 59 190148.5 60 159009.5 61 128103.5 62 100274.0 63 78647.5 64 62258.0 65 49080.5 66 39070.0 67 31395.0 68 24711.0 69 19176.0 70 15014.0 71 11918.0 72 9730.5 73 7631.5 74 5629.0 75 4277.0 76 3233.0 77 2327.5 78 1630.0 79 1114.0 80 723.0 81 498.5 82 344.5 83 224.5 84 145.0 85 98.0 86 67.0 87 43.0 88 30.0 89 17.5 90 9.5 91 8.5 92 6.0 93 5.0 94 4.5 95 4.0 96 3.0 97 3.5 98 2.5 99 5.0 100 10.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.075114166686786 2 0.01708041017487962 3 0.017963258380121284 4 0.020233439479314146 5 0.012179701770272808 6 0.006846577918200691 7 0.022665776371306498 8 0.011188749703164812 9 0.0287556272564429 10-11 0.01492734159270861 12-13 0.024647680505522487 14-15 0.019233478757050625 16-17 0.020404603927269165 18-19 0.01340487887142451 20-21 0.013296775009558184 22-23 9.690024788215526 24-25 0.09312246834268492 26-27 0.05296188365934455 28-29 0.04127765792262574 30-31 0.038890364306411025 32-33 0.04062002609627225 34-35 0.033458145247628114 36-37 0.04151188295666945 38-39 0.05164662000663757 40-41 0.07357368665519085 42-43 0.07287101155305972 44-45 0.06577219129050427 46-47 0.10206806291212345 48-49 0.09502329458050117 50-51 0.09294229523957437 52-53 0.05562844558538061 54-55 0.03685440824126187 56-57 0.03855704406565652 58-59 0.04937643890744472 60-61 0.0513583430416607 62-63 0.06188946091847203 64-65 0.036593157241751585 66-67 0.04115153675044836 68-69 0.03788139492899198 70-71 0.01880106330958532 72-73 0.022116248406819337 74-75 0.028314203153822066 76-77 0.024872896884410665 78-79 0.021503659856243484 80-81 0.03609768120819759 82-83 0.02596294415822946 84-85 0.016774115899591692 86-87 0.024377420850856667 88-89 0.03048528904630413 90-91 0.013810268353423236 92-93 0.014125571283866689 94-95 0.010900472738187943 96-97 0.022440559992418316 98-99 0.03188163059541085 100-101 0.04322352743621962 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5550218.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.29483889733915 #Duplication Level Percentage of deduplicated Percentage of total 1 89.78673132705556 61.31970351099935 2 5.5720994091111935 7.610912629304154 3 1.5039037421461465 3.081265913609297 4 0.7474465971611455 2.0418697974993916 5 0.4907280916840155 1.6757097981979256 6 0.35333123603563044 1.4478419905470659 7 0.2603402968197604 1.2445929040853694 8 0.22219757065564824 1.2139957833046104 9 0.1705843868489542 1.0485029896425635 >10 0.8361043966823651 9.450089882299464 >50 0.02946646363812772 1.386827926417838 >100 0.02312015730642427 3.078788050089055 >500 0.001648812554542625 0.7620606694946048 >1k 0.0021623645181399645 2.3851528111878513 >5k 5.405911294916981E-5 0.2571449032423341 >10k+ 8.108866942375472E-5 1.9955404400791674 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 45721 0.8237694447317204 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA 38072 0.685955038162465 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24309 0.4379827963514226 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA 7606 0.13703966222588015 No Hit AAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAA 6324 0.11394147040710834 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.8017310311054447E-5 3 0.0 0.0 0.0 0.0 1.8017310311054447E-5 4 1.8017310311054447E-5 0.0 0.0 0.0 7.206924124421779E-5 5 3.6034620622108895E-5 0.0 0.0 0.0 7.206924124421779E-5 6 3.6034620622108895E-5 0.0 0.0 0.0 7.206924124421779E-5 7 3.6034620622108895E-5 0.0 0.0 0.0 7.206924124421779E-5 8 3.6034620622108895E-5 0.0 0.0 0.0 7.206924124421779E-5 9 3.6034620622108895E-5 0.0 0.0 0.0 7.206924124421779E-5 10-11 3.6034620622108895E-5 0.0 0.0 0.0 7.206924124421779E-5 12-13 5.405193093316335E-5 0.0 0.0 0.0 7.206924124421779E-5 14-15 6.306058608869057E-5 0.0 0.0 0.0 7.206924124421779E-5 16-17 8.107789639974502E-5 0.0 0.0 0.0 7.206924124421779E-5 18-19 1.171125170218539E-4 0.0 0.0 0.0 7.206924124421779E-5 20-21 1.4413848248843558E-4 0.0 0.0 0.0 7.206924124421779E-5 22-23 1.4413848248843558E-4 0.0 0.0 0.0 7.206924124421779E-5 24-25 2.252163788881806E-4 0.0 0.0 0.0 1.081038618663267E-4 26-27 3.153029304434528E-4 0.0 0.0 0.0 1.081038618663267E-4 28-29 4.6845006808741567E-4 0.0 0.0 0.0 1.081038618663267E-4 30-31 5.945712402647969E-4 0.0 0.0 0.0 1.081038618663267E-4 32-33 6.936664469755963E-4 0.0 0.0 0.0 1.081038618663267E-4 34-35 7.837529985308684E-4 0.0 0.0 0.0 1.2612117217738114E-4 36-37 9.459087913303585E-4 0.0 0.0 0.0 1.4413848248843558E-4 38-39 0.0011891424805295938 0.0 0.0 0.0 1.531471376439628E-4 40-41 0.0013152636527069747 0.0 0.0 0.0 1.6215579279949005E-4 42-43 0.0014594021351954103 0.0 0.0 1.8017310311054447E-5 1.6215579279949005E-4 44-45 0.001612549272839373 0.0 0.0 1.8017310311054447E-5 1.6215579279949005E-4 46-47 0.0017837137207943905 0.0 0.0 1.8017310311054447E-5 1.6215579279949005E-4 48-49 0.001963886823904935 0.0 0.0 1.8017310311054447E-5 1.6215579279949005E-4 50-51 0.002189103202793115 0.0 0.0 3.6034620622108895E-5 1.7116444795501728E-4 52-53 0.0025044061332365683 0.0 0.0 3.6034620622108895E-5 1.9819041342159893E-4 54-55 0.0027926830982134398 0.0 0.0 3.6034620622108895E-5 2.0719906857712616E-4 56-57 0.003044925442568202 0.0 0.0 3.6034620622108895E-5 2.162077237326534E-4 58-59 0.0032971677869229644 0.0 0.0 3.6034620622108895E-5 2.162077237326534E-4 60-61 0.003468332234877981 0.0 0.0 3.6034620622108895E-5 2.162077237326534E-4 62-63 0.003693548613766162 0.0 0.0 3.6034620622108895E-5 2.3422503404370784E-4 64-65 0.003972816923587506 0.0 0.0 3.6034620622108895E-5 2.3422503404370784E-4 66-67 0.004207041957631214 0.0 0.0 3.6034620622108895E-5 2.3422503404370784E-4 68-69 0.004567388163852303 0.0 0.0 3.6034620622108895E-5 2.522423443547623E-4 70-71 0.00490070840460681 0.0 0.0 3.6034620622108895E-5 2.522423443547623E-4 72-73 0.0052880805762944814 0.0 0.0 3.6034620622108895E-5 2.522423443547623E-4 74-75 0.005639418127360043 0.0 0.0 3.6034620622108895E-5 2.79268309821344E-4 76-77 0.005990755678425604 0.0 0.0 3.6034620622108895E-5 2.8827696497687116E-4 78-79 0.006531274987757237 9.008655155527224E-6 0.0 3.6034620622108895E-5 3.062942752879256E-4 80-81 0.007486192434243123 1.8017310311054447E-5 0.0 3.6034620622108895E-5 3.062942752879256E-4 82-83 0.008233910812151883 1.8017310311054447E-5 0.0 3.6034620622108895E-5 3.243115855989801E-4 84-85 0.009269906155037514 1.8017310311054447E-5 1.8017310311054447E-5 3.6034620622108895E-5 3.243115855989801E-4 86-87 0.010143745705123653 1.8017310311054447E-5 1.8017310311054447E-5 3.6034620622108895E-5 3.243115855989801E-4 88-89 0.01148603532329721 1.8017310311054447E-5 1.8017310311054447E-5 3.6034620622108895E-5 3.243115855989801E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTATC 250890 0.0 62.928654 7 GTGGTAT 252610 0.0 62.91712 6 AGTGGTA 257375 0.0 61.91752 5 GTATCAA 256610 0.0 61.83073 9 CAGTGGT 257260 0.0 61.792923 4 GGTATCA 256740 0.0 61.70644 8 AAGCAGT 269870 0.0 59.685696 1 AGCAGTG 275935 0.0 58.213806 2 GCAGTGG 278805 0.0 57.559555 3 GTACTTT 8195 0.0 43.85752 22-23 GTCACCG 8865 0.0 43.75222 22-23 AGTACTT 8550 0.0 43.349854 20-21 TCACCGG 9120 0.0 43.07005 22-23 CTTAACG 11045 0.0 42.62566 52-53 TAACGCC 11080 0.0 42.551525 54-55 GAGTACT 10170 0.0 42.12832 20-21 CTATTCG 11165 0.0 41.93988 90-91 AGACTCG 11245 0.0 41.846905 66-67 TCGGTTA 10860 0.0 41.827625 94-95 GGTCTAT 10765 0.0 41.701748 36-37 >>END_MODULE