FastQCFastQC Report
Sat 18 Jun 2016
SRR799685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR799685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5550218
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1949323.512150333554466No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA554080.9983031297149049No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA323700.5832203347688325No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA68980.12428340652565358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2242100.057.5224727
GTATCAA2253300.057.420029
GTGGTAT2247800.057.3259856
GGTATCA2264100.056.9819958
AGTGGTA2335750.055.1022765
CAGTGGT2363550.055.0589834
AAGCAGT2412050.054.0315131
AGCAGTG2459400.052.8565182
GCAGTGG2501100.052.068123
AGTACTT321400.042.56625720-21
GTACTTT317050.042.3242322-23
TAACGCC91800.041.82978454-55
CTTAACG92200.041.6996952-53
AGAGTAC449500.041.56743218-19
GAGTACT342250.041.3629420-21
GTGGTCT37000.040.6796576
AACGCCC95450.040.5042354-55
TACTTTT333550.040.2792422-23
GGTCTAT87950.040.10267636-37
CTATACC78700.039.87621738-39
Produced by FastQC (version 0.11.5)