##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR799685_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5550218 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29570550201812 31.0 31.0 34.0 30.0 34.0 2 31.56604191042586 31.0 31.0 34.0 30.0 34.0 3 31.74721569495108 31.0 31.0 34.0 30.0 34.0 4 35.25622525097212 37.0 35.0 37.0 33.0 37.0 5 35.20100741988873 37.0 35.0 37.0 33.0 37.0 6 34.918589504051916 36.0 35.0 37.0 32.0 37.0 7 35.01670799237075 36.0 35.0 37.0 32.0 37.0 8 34.763982243580344 36.0 35.0 37.0 32.0 37.0 9 36.23327678300204 38.0 35.0 39.0 32.0 39.0 10-11 36.40736661154571 38.0 35.0 39.0 32.0 39.0 12-13 36.309196413546275 38.0 35.0 39.0 32.0 39.0 14-15 37.618390394755664 39.0 37.0 40.0 32.0 41.0 16-17 36.00125841903868 39.0 36.0 40.0 27.0 41.0 18-19 31.992261114788647 38.0 29.0 40.0 10.0 41.0 20-21 36.183879984533945 38.0 33.5 40.0 29.0 41.0 22-23 36.97413669156779 39.0 36.0 40.0 31.5 41.0 24-25 35.80693470058293 38.0 34.0 40.0 28.0 41.0 26-27 34.49695750689432 38.0 32.5 40.0 24.0 41.0 28-29 34.34234610244138 38.0 32.5 40.0 22.5 41.0 30-31 33.86645542571481 37.5 31.5 40.0 20.0 41.0 32-33 33.62093407862538 37.0 31.0 40.0 20.0 41.0 34-35 33.7545354975246 37.0 31.0 40.0 21.0 41.0 36-37 33.660523965004614 37.0 31.0 40.0 20.5 41.0 38-39 33.28680675245549 36.0 31.0 40.0 19.5 41.0 40-41 33.15965066597384 36.0 30.5 39.5 19.5 41.0 42-43 33.18778829948661 36.0 31.0 40.0 19.5 41.0 44-45 32.79349315648503 36.0 30.0 39.5 18.0 41.0 46-47 32.83142725204668 36.0 30.0 39.0 19.0 41.0 48-49 32.62166134735608 35.0 30.0 39.0 18.0 41.0 50-51 31.287600234801587 35.0 28.0 39.0 11.0 40.5 52-53 31.303465917915297 35.0 27.5 39.0 12.5 40.0 54-55 31.29210222012901 35.0 28.0 39.0 13.0 40.0 56-57 30.864996113666166 35.0 27.0 38.5 11.0 40.0 58-59 30.909519950387534 34.5 27.0 38.0 12.5 40.0 60-61 30.774469759566202 34.0 27.5 38.0 13.0 40.0 62-63 30.197986727728534 34.0 26.0 37.0 10.5 40.0 64-65 30.206750077204177 34.0 26.0 36.5 12.0 39.5 66-67 30.00476179494211 34.0 26.5 36.0 11.5 39.0 68-69 29.7476485608313 33.0 26.0 36.0 11.5 39.0 70-71 29.350113455003026 33.0 26.0 35.0 10.5 38.5 72-73 29.247475414479215 33.0 26.0 35.0 11.0 37.5 74-75 29.104069876174236 33.0 26.0 35.0 11.0 37.0 76-77 28.84881494744891 33.0 26.0 35.0 10.0 37.0 78-79 28.706743140539707 33.0 25.5 35.0 10.0 36.0 80-81 28.471307433329645 33.0 25.0 35.0 10.0 36.0 82-83 28.44307520893774 33.0 25.0 35.0 10.0 35.5 84-85 28.180152833636445 32.5 25.0 35.0 10.0 35.0 86-87 28.014405740459203 32.0 25.0 35.0 10.0 35.0 88-89 27.97328861677145 32.0 25.0 35.0 10.0 35.0 90-91 27.881373308219604 32.0 24.5 35.0 10.0 35.0 92-93 27.844611779212997 32.0 24.5 35.0 10.0 35.0 94-95 27.844866003461487 32.0 24.5 35.0 10.0 35.0 96-97 27.668615629151866 32.0 24.0 35.0 10.0 35.0 98-99 27.557510533820473 32.0 24.0 35.0 10.0 35.0 100-101 25.827062738796926 30.5 21.0 33.5 7.5 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2105 1 -0.22827330611584884 2105 2 -0.06422070845777839 2105 3 -0.10993785695448466 2105 4 -0.39119430500677765 2105 5 -0.20781832574165549 2105 6 -0.03640583300500566 2105 7 -0.21569221268535443 2105 8 0.047065295053982936 2105 9 -0.05999256114197493 2105 10-11 0.062449503552116425 2105 12-13 0.12544703956871217 2105 14-15 -0.09334780878990756 2105 16-17 1.4437963201400734 2105 18-19 6.0313772533869425 2105 20-21 1.1850595414523681 2105 22-23 0.09199140997360189 2105 24-25 -0.39761903952188504 2105 26-27 -0.1022253326635294 2105 28-29 0.762863687456651 2105 30-31 0.5260410460891478 2105 32-33 0.09010723487284622 2105 34-35 0.018673779493660447 2105 36-37 0.11778852910485682 2105 38-39 -0.004951741338679483 2105 40-41 -0.19459226055669632 2105 42-43 -0.12207517292477377 2105 44-45 0.9573377798296079 2105 46-47 0.2486381587366182 2105 48-49 -0.08499339583631382 2105 50-51 1.8606670892411898 2105 52-53 1.078151500167845 2105 54-55 0.11701468428229234 2105 56-57 0.7611098420677749 2105 58-59 -0.053998118987411914 2105 60-61 -0.4431469874337921 2105 62-63 -0.2256172667566716 2105 64-65 -0.34436662959575415 2105 66-67 -0.4434323542666121 2105 68-69 -0.3112639181619379 2105 70-71 -0.3971039235700431 2105 72-73 -0.1625176648483162 2105 74-75 -0.17154731641175047 2105 76-77 -0.26585102428940743 2105 78-79 -0.25633094179847404 2105 80-81 -0.29149531999832234 2105 82-83 -0.4068253634849306 2105 84-85 -0.48607672785244205 2105 86-87 -0.309467119251309 2105 88-89 -0.4937369876371207 2105 90-91 -0.49183072581848464 2105 92-93 -0.49295914478040714 2105 94-95 -0.5426506129258115 2105 96-97 -0.510115126431721 2105 98-99 -0.3740370835708795 2105 100-101 -0.6201585805001351 2106 1 -0.0815945892901837 2106 2 -6.352073115465373E-4 2106 3 0.003775725284725695 2106 4 -0.05227888035299344 2106 5 -0.07313476023539067 2106 6 -0.13430469471875028 2106 7 -0.15695396629317315 2106 8 0.07253562697817983 2106 9 0.24027102545481682 2106 10-11 0.15369364919904882 2106 12-13 0.020839066972186515 2106 14-15 -0.11659176802609039 2106 16-17 0.7622254989151926 2106 18-19 5.900396850429551 2106 20-21 1.1199367770180189 2106 22-23 0.20149485346938434 2106 24-25 -0.2723528063527567 2106 26-27 -0.6174151444306943 2106 28-29 0.2971821635633063 2106 30-31 0.3369174002311368 2106 32-33 -0.17997189413770087 2106 34-35 -0.2605999870510445 2106 36-37 -0.1392955420418147 2106 38-39 -0.0999078422582329 2106 40-41 -2.0018643108817926 2106 42-43 -1.8194732346132199 2106 44-45 -0.21822029422218492 2106 46-47 -0.2169326869027799 2106 48-49 -0.4429841781600956 2106 50-51 1.5788256097182085 2106 52-53 1.0987111771719533 2106 54-55 0.2732647374388719 2106 56-57 0.9895750357393354 2106 58-59 -0.9416670706115724 2106 60-61 -0.814025688970375 2106 62-63 0.13117870573058354 2106 64-65 0.01236688078275705 2106 66-67 -0.8018889633380617 2106 68-69 -1.3127656103172463 2106 70-71 -1.8172799251325742 2106 72-73 -2.680208600032909 2106 74-75 -1.225161005804619 2106 76-77 -0.5527492455226408 2106 78-79 -0.3462649090684238 2106 80-81 -0.2825502307207799 2106 82-83 -0.04881295581353129 2106 84-85 -1.1334263682738168 2106 86-87 -1.3188524325659472 2106 88-89 -0.7292607873265133 2106 90-91 -0.34473210953055045 2106 92-93 -0.2640838324573558 2106 94-95 -0.40156240492143525 2106 96-97 -0.19514886747727545 2106 98-99 -0.271055101975616 2106 100-101 -0.3617003054389194 2107 1 -0.08008839748499241 2107 2 -0.2445148456429962 2107 3 -0.06552951729674206 2107 4 -0.1532749261686206 2107 5 -0.021599345669493175 2107 6 -0.01532908169394176 2107 7 -0.01418557041747448 2107 8 0.2346500789340027 2107 9 0.246080467070378 2107 10-11 0.2493547892130863 2107 12-13 0.1791781068320688 2107 14-15 0.14696219558695844 2107 16-17 1.6077804509108589 2107 18-19 6.18095741811927 2107 20-21 1.3723031695979913 2107 22-23 0.2722730785581078 2107 24-25 -0.06206277194529264 2107 26-27 0.12561084969682668 2107 28-29 0.7845199682149939 2107 30-31 0.6984755770788524 2107 32-33 0.18522444747627986 2107 34-35 0.23818473388296724 2107 36-37 0.2085128695986569 2107 38-39 0.14143460926444362 2107 40-41 0.1270568632016733 2107 42-43 0.1395473979237991 2107 44-45 1.111326596339893 2107 46-47 0.4383263851382537 2107 48-49 0.1315021484507568 2107 50-51 1.979501745338311 2107 52-53 1.3140514982390918 2107 54-55 0.6213874700203057 2107 56-57 1.2394599634786019 2107 58-59 0.5013919899552803 2107 60-61 -0.05457715862733181 2107 62-63 0.25420843308782537 2107 64-65 0.00196884170704692 2107 66-67 -0.049759325155864786 2107 68-69 -0.10170141369170338 2107 70-71 0.03967391493122108 2107 72-73 0.22210576500232904 2107 74-75 0.09924817359927829 2107 76-77 -0.07540915953966376 2107 78-79 0.09861094352388378 2107 80-81 0.20322793807737938 2107 82-83 0.007742215601595603 2107 84-85 0.020776047517991003 2107 86-87 0.036215712842349035 2107 88-89 -0.07176778320539157 2107 90-91 -0.07464219360618429 2107 92-93 -0.052347379427292395 2107 94-95 -7.773928011189923E-5 2107 96-97 -1.216346488507451 2107 98-99 -1.2375184090454248 2107 100-101 -0.5469221529309074 2108 1 0.11081118876613161 2108 2 0.18805233246241215 2108 3 0.13967117824201125 2108 4 0.15764308418704331 2108 5 0.031072722412453402 2108 6 0.15182128673349382 2108 7 0.12361528976073544 2108 8 0.2135111569239001 2108 9 0.4092318066008005 2108 10-11 0.35674924101694927 2108 12-13 0.24812406862636038 2108 14-15 0.20998616787219504 2108 16-17 -2.090258897021627 2108 18-19 5.3244390770854935 2108 20-21 1.0970374103392189 2108 22-23 0.24070505871093673 2108 24-25 -0.07400701266556098 2108 26-27 -0.24771565758876335 2108 28-29 -3.3517658101661922 2108 30-31 -1.8965381122041762 2108 32-33 -0.6963185296115242 2108 34-35 -0.2792888922899479 2108 36-37 -0.09746930715327551 2108 38-39 0.0393960421032844 2108 40-41 0.14143758063448786 2108 42-43 0.1096665749919552 2108 44-45 1.166788106693886 2108 46-47 0.35458923575633605 2108 48-49 0.1416689101053592 2108 50-51 1.9687633071152817 2108 52-53 1.2484356653046085 2108 54-55 0.8119517992723146 2108 56-57 1.510098975529722 2108 58-59 0.8658754601201437 2108 60-61 0.057742960754858075 2108 62-63 0.5164993796422692 2108 64-65 0.22796020697699504 2108 66-67 0.1980047504714939 2108 68-69 0.24471643454124958 2108 70-71 0.2470262905783187 2108 72-73 0.3182936816606734 2108 74-75 0.16316928315876922 2108 76-77 0.12634015108356778 2108 78-79 0.15574826947442233 2108 80-81 0.2184601549822176 2108 82-83 0.1755092939680729 2108 84-85 0.17303620445441936 2108 86-87 0.2620216397779096 2108 88-89 0.10965817013405754 2108 90-91 0.10456015226236914 2108 92-93 0.16504357014545334 2108 94-95 0.1205817810072034 2108 96-97 0.025310747632776298 2108 98-99 0.07565129877606225 2108 100-101 0.10083406251336768 2109 1 0.14232440401048052 2109 2 0.2508945804655518 2109 3 0.1843489602228594 2109 4 0.17160572160923238 2109 5 0.2158465001913612 2109 6 0.1355974120532224 2109 7 0.1252695051358259 2109 8 0.19494478329323783 2109 9 0.15731421187391703 2109 10-11 0.20288689571032847 2109 12-13 0.21559021293681724 2109 14-15 0.11740871094551864 2109 16-17 -1.9280503583161703 2109 18-19 5.42877071975979 2109 20-21 1.361904028615406 2109 22-23 0.4450746214537489 2109 24-25 0.16162955176267246 2109 26-27 -0.0702998351236701 2109 28-29 0.4200504456091352 2109 30-31 0.3954851478704384 2109 32-33 -0.028070775285542027 2109 34-35 0.1513444654325795 2109 36-37 0.16563534880885555 2109 38-39 0.18268477059127264 2109 40-41 0.19412354706813062 2109 42-43 0.24057815046114683 2109 44-45 1.071764323965553 2109 46-47 0.46329377324799026 2109 48-49 0.23739337769272595 2109 50-51 2.0139493814746103 2109 52-53 1.3590533843426549 2109 54-55 0.7756825684376558 2109 56-57 1.5158168055950085 2109 58-59 0.808476469624086 2109 60-61 0.3641711439961277 2109 62-63 0.6065364533170161 2109 64-65 0.4160292067828877 2109 66-67 0.3102124657119809 2109 68-69 0.3536940406580875 2109 70-71 0.37657776819620636 2109 72-73 0.44107543901232305 2109 74-75 0.4227842166416629 2109 76-77 0.30980281361650697 2109 78-79 0.4594148926913988 2109 80-81 0.42164426717739545 2109 82-83 0.2838094892057015 2109 84-85 0.3084316267830971 2109 86-87 0.3449682486517993 2109 88-89 0.3353744903492064 2109 90-91 0.23211586761834369 2109 92-93 0.21312313766954105 2109 94-95 0.13765208028519282 2109 96-97 0.25550570025112407 2109 98-99 0.25103961613068293 2109 100-101 0.09463648397866464 2110 1 0.09539267222761438 2110 2 0.13052579998026914 2110 3 0.08625773868534026 2110 4 0.18181253338278225 2110 5 0.1939038710936032 2110 6 0.2728565556849887 2110 7 0.19686822639934576 2110 8 -0.1073574585888295 2110 9 -0.22164773051557063 2110 10-11 -4.353039695964441 2110 12-13 -2.8234938817099007 2110 14-15 -1.0436245059750178 2110 16-17 0.7032881726312965 2110 18-19 5.860346809512173 2110 20-21 0.8084396386645523 2110 22-23 0.2543230292958256 2110 24-25 0.16282410238841294 2110 26-27 0.07177333936110131 2110 28-29 0.5258050650030057 2110 30-31 0.41971300260030375 2110 32-33 0.1683657210599776 2110 34-35 0.14362236683301433 2110 36-37 0.13959859933205365 2110 38-39 0.14446951659967766 2110 40-41 0.22096700433142047 2110 42-43 -0.3548738742636317 2110 44-45 0.7470699838646198 2110 46-47 0.41360345481285776 2110 48-49 0.1649816796313175 2110 50-51 1.6595616978555796 2110 52-53 1.382247954016048 2110 54-55 0.8624598771188907 2110 56-57 1.5217069534167749 2110 58-59 0.9644353397420495 2110 60-61 0.5334384604430795 2110 62-63 0.6933292002303872 2110 64-65 0.38157452376930934 2110 66-67 0.41634819034092985 2110 68-69 0.3795520473216776 2110 70-71 0.4287157366499841 2110 72-73 0.5682406194082574 2110 74-75 0.37817743955917393 2110 76-77 0.25859541974941536 2110 78-79 0.36057850732890984 2110 80-81 0.21065082871734475 2110 82-83 0.13815636092721206 2110 84-85 0.20815238728434338 2110 86-87 0.2996746412121283 2110 88-89 0.2859048031217064 2110 90-91 0.08632111034065915 2110 92-93 0.0903353989743998 2110 94-95 0.2097198565812306 2110 96-97 0.11717389077304219 2110 98-99 0.08646930899041294 2110 100-101 -0.009352642490700447 2111 1 0.09394486273248503 2111 2 0.15624286838881218 2111 3 0.0811946010922 2111 4 0.2144975014838053 2111 5 0.21049570749056556 2111 6 0.21727485014558567 2111 7 0.16197357898792575 2111 8 0.4283452617531154 2111 9 0.47286731175064745 2111 10-11 0.463818772977568 2111 12-13 0.46674096875413795 2111 14-15 0.237665685518067 2111 16-17 1.437554479565513 2111 18-19 -21.550201310623745 2111 20-21 -4.391729695898803 2111 22-23 -0.6134657421446619 2111 24-25 0.32096285994607143 2111 26-27 0.4270321937007253 2111 28-29 -7.490239010550731 2111 30-31 -6.213334661414848 2111 32-33 -1.2407253153926447 2111 34-35 0.16833486043265822 2111 36-37 0.5157035447903411 2111 38-39 0.5851704377053366 2111 40-41 0.6636604092438745 2111 42-43 0.7525926892598633 2111 44-45 1.6246211481232962 2111 46-47 0.9819290185995868 2111 48-49 0.6763443916275023 2111 50-51 2.448901082036059 2111 52-53 1.8468727124821847 2111 54-55 1.2653530706644993 2111 56-57 1.9437650269675188 2111 58-59 1.1605864772245127 2111 60-61 0.7048118128737038 2111 62-63 0.9308615683145831 2111 64-65 0.7153353137509306 2111 66-67 0.49896787574587265 2111 68-69 0.5913162185657761 2111 70-71 0.6918366333324926 2111 72-73 0.6893618744494816 2111 74-75 0.5514166769258253 2111 76-77 0.39846385148988617 2111 78-79 0.4123317827518065 2111 80-81 0.5599781501779866 2111 82-83 0.36797692413880867 2111 84-85 0.36295332000936753 2111 86-87 0.4341849417486543 2111 88-89 0.44380270176725034 2111 90-91 0.3960414095815743 2111 92-93 0.44110841386835276 2111 94-95 0.4401190897321001 2111 96-97 0.45956912506325764 2111 98-99 0.42543387441158487 2111 100-101 0.11808789551553289 2112 1 -0.044926285640045194 2112 2 -0.022970721506524683 2112 3 -0.12316949198345384 2112 4 0.13541770021863897 2112 5 0.19018498169446474 2112 6 0.17097011615263824 2112 7 0.13649384083020522 2112 8 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2215 32-33 -0.6905458153273827 2215 34-35 -0.6036201064696414 2215 36-37 -0.5960418170361379 2215 38-39 -0.6213003362287353 2215 40-41 -0.8132843842785888 2215 42-43 -0.7587823929102839 2215 44-45 -0.11239355530111794 2215 46-47 -0.4737723313207667 2215 48-49 -0.7248837467392804 2215 50-51 -6.546260045223093 2215 52-53 -7.40749713763169 2215 54-55 -2.108101761230497 2215 56-57 0.08999094219383608 2215 58-59 -0.2897751203382697 2215 60-61 -0.614079087278995 2215 62-63 -0.36743602432412814 2215 64-65 -0.7271567142579123 2215 66-67 -0.5287347017433923 2215 68-69 -0.7108182738976048 2215 70-71 -0.9126588333683685 2215 72-73 -0.7844887840589543 2215 74-75 -0.7397872774509189 2215 76-77 -0.786088964659605 2215 78-79 -0.9163662724292863 2215 80-81 -0.7892188725064351 2215 82-83 -0.763055026016616 2215 84-85 -0.7246721638934339 2215 86-87 -0.7067849317848527 2215 88-89 -0.865725521692017 2215 90-91 -0.7805247260953294 2215 92-93 -0.7246288674782413 2215 94-95 -0.5589530487309133 2215 96-97 -0.6990017124810528 2215 98-99 -0.43407276453540433 2215 100-101 -0.25493343074382224 2216 1 -0.03471450534974707 2216 2 0.002064168686040091 2216 3 -0.10612702264399587 2216 4 0.059720763902092244 2216 5 0.12836863732054837 2216 6 0.06496242362874938 2216 7 -0.02574309299701838 2216 8 0.1313616617804314 2216 9 0.08613939215625521 2216 10-11 0.12700667120844855 2216 12-13 -0.5159550464444607 2216 14-15 -0.38302221256409297 2216 16-17 1.0199710021807817 2216 18-19 5.6386891029057935 2216 20-21 1.224337894071006 2216 22-23 0.13786864065502158 2216 24-25 -0.3835269103224306 2216 26-27 -0.4751767897526662 2216 28-29 0.3709016564295595 2216 30-31 0.17904671420553342 2216 32-33 -0.3197195762099696 2216 34-35 -0.5657426524567128 2216 36-37 -0.5854389608307287 2216 38-39 -0.563171332286899 2216 40-41 -0.6851907667128714 2216 42-43 -0.7600742637249525 2216 44-45 0.35044724557306495 2216 46-47 -0.2935203731364595 2216 48-49 -0.5845782470300698 2216 50-51 1.2621844674872698 2216 52-53 0.7088942182288953 2216 54-55 0.036954370773742085 2216 56-57 0.7239434680737915 2216 58-59 -0.019469098567046927 2216 60-61 -0.4113855921546943 2216 62-63 -0.0999277787765358 2216 64-65 -0.3731143279260465 2216 66-67 -0.5654890058598774 2216 68-69 -0.5584894927405841 2216 70-71 -0.6381121762193587 2216 72-73 -0.5514383890786192 2216 74-75 -0.4411622741277519 2216 76-77 -0.535205658042905 2216 78-79 -0.4927063733200754 2216 80-81 -0.5171941340768633 2216 82-83 -0.7358073764834643 2216 84-85 -0.7099322750707451 2216 86-87 -0.6404837132616024 2216 88-89 -0.5103705971234298 2216 90-91 -0.44693709523092906 2216 92-93 -0.3815616952787657 2216 94-95 -0.41588556705733026 2216 96-97 -0.3961262844829321 2216 98-99 -0.5201613539742809 2216 100-101 -0.19797115264944765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 96.0 8 332.0 9 497.0 10 863.0 11 1464.0 12 2844.0 13 6221.0 14 11712.0 15 19642.0 16 32118.0 17 49405.0 18 65113.0 19 75281.0 20 83372.0 21 94120.0 22 107591.0 23 124166.0 24 143797.0 25 162240.0 26 175292.0 27 185601.0 28 199332.0 29 222335.0 30 251992.0 31 290491.0 32 336742.0 33 392503.0 34 464354.0 35 571920.0 36 631623.0 37 554798.0 38 259068.0 39 33241.0 40 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 16.006857324508122 43.47479223218599 21.215014390614122 19.30333605269176 2 17.123777840798326 45.32935463075504 18.077217867838705 19.469649660607928 3 43.1734573308652 20.17165812225754 17.988212354902096 18.666672191975163 4 18.250292150686693 19.68383944558574 18.132909374010172 43.93295902971739 5 18.349379231996828 44.85409021647988 19.686974240607334 17.10955631091596 6 43.3959955702541 20.123666742706074 18.343209446761062 18.13712824027876 7 17.9336199046596 20.068328847623643 44.59358172958252 17.404469518134245 8 43.02618383638264 20.61115437267509 18.78695575561176 17.575706035330505 9 42.45294509152613 20.343705418417798 19.37388765630467 17.829461833751395 10-11 16.918180118783212 32.91463396387905 32.20681805604302 17.960367861294724 12-13 16.93810945804291 20.48720428639019 31.99215778551401 30.58252847005289 14-15 16.925407254273615 45.69502495217305 19.565042310049805 17.814525483503534 16-17 29.87208159172735 20.04301583424048 19.468748659257763 30.616153914774408 18-19 16.811544315865167 34.125043857879355 19.486708893827696 29.576702932427786 20-21 29.686605046253145 32.76637652185491 19.68769869718607 17.85931973470587 22-23 29.56776284602926 20.071125146998757 32.378851133207945 17.982260873764037 24-25 22.9324828907313 28.58077571146883 22.681276089112338 25.80546530868753 26-27 24.47862774176612 23.446378325572965 27.91186782335584 24.16312610930507 28-29 36.681676490137285 19.912694141394194 25.578539147549893 17.82709022091863 30-31 29.649137505450273 24.16899992432678 27.193027973867512 18.988834596355435 32-33 23.45620433484728 26.244337124679113 29.646368480756674 20.653090059716938 34-35 21.43938638436254 25.364418065741777 28.721747898650936 24.474447651244745 36-37 24.98861527820206 23.051045984546686 30.920922420564374 21.039416316686882 38-39 25.950056147308192 22.911480041242257 28.996397833745412 22.142065977704135 40-41 20.574718763397662 26.581718001529865 31.337549596404656 21.506013638667824 42-43 20.750380192666988 23.984249785118283 31.47983571244471 23.78553430977002 44-45 20.736105272175916 28.321602043962695 28.056834699071825 22.88545798478957 46-47 23.634626245434895 22.897515990421468 28.546538502459274 24.921319261684367 48-49 21.666738286023566 25.559642213115602 28.303249149508876 24.470370351351953 50-51 23.56606762009951 26.969444365564627 27.86534268133931 21.599145332996557 52-53 23.98238194693684 23.200637596807045 30.427491470602224 22.38948898565389 54-55 22.74634636745572 25.62503976773823 28.542491941920012 23.086121922886036 56-57 23.653664026454283 25.3160841402878 26.74446511945151 24.285786713806413 58-59 27.742391633668678 23.771416710338155 26.103541836656646 22.38264981933652 60-61 24.378509922895546 25.833933401002167 25.994949431705212 23.792607244397075 62-63 24.44617270241979 26.971941137908807 26.107081943061296 22.47480421661011 64-65 21.939529986772563 25.67948159757328 28.143867826155265 24.237120589498893 66-67 24.10011642785923 25.413596366845407 28.407379674095683 22.078907531199675 68-69 26.6293858118045 24.664416002090007 26.719697669233383 21.986500516872102 70-71 21.30187189906415 26.08092337867574 29.62561838541341 22.991586336846698 72-73 22.088958412610555 24.26677578095271 28.685327804107423 24.958938002329308 74-75 22.30863996778441 27.999845048046367 27.39538828752416 22.296126696645064 76-77 23.78807235615889 23.810090082590797 27.821586506917427 24.580251054332884 78-79 21.92579880137565 25.7752432946179 26.454625919744867 25.844331984261583 80-81 24.0359378882189 25.991403532889972 27.161129886254283 22.81152869263684 82-83 23.987172132269954 24.597259799095657 28.523771806798575 22.891796261835815 84-85 25.003225179898376 25.23979843166168 26.35722416874141 23.399752219698534 86-87 23.480780867539913 25.12500649750014 26.785546821053575 24.60866581390637 88-89 26.22000091708564 24.247519071642675 25.645444061784907 23.88703594948678 90-91 23.986596508177055 26.06110000990055 25.58376547276578 24.368538009156616 92-93 23.42657531650108 27.28649577367952 26.85458481090292 22.432344098916477 94-95 21.9363404520903 26.163695285383138 27.501647914627885 24.398316347898675 96-97 24.58636759853397 24.46056172928703 27.343754785848052 23.60931588633095 98-99 26.517778019410255 24.463252339708557 26.361306476574175 22.657663164307014 100-101 22.104303147471764 26.98592005765837 27.875247006441185 23.03452978842868 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 49757.0 1 32274.0 2 10197.0 3 4537.5 4 3078.5 5 2410.0 6 1979.5 7 1737.5 8 1547.0 9 1452.5 10 1680.5 11 8775.5 12 33284.0 13 33930.0 14 15165.0 15 12923.0 16 12575.5 17 13048.5 18 13876.0 19 14575.5 20 15430.5 21 16552.0 22 17580.0 23 18980.5 24 20947.0 25 23304.0 26 25727.0 27 28360.5 28 31577.0 29 35650.0 30 40334.5 31 45348.0 32 50749.0 33 57287.5 34 64974.0 35 73070.0 36 81520.0 37 88401.0 38 92268.5 39 94395.5 40 97564.0 41 102545.5 42 108509.5 43 116344.0 44 125924.5 45 135459.5 46 147391.0 47 161948.0 48 174799.0 49 183040.5 50 195477.5 51 217930.0 52 253987.5 53 304727.0 54 320310.0 55 299400.5 56 276121.5 57 240421.0 58 197981.5 59 160521.0 60 126995.0 61 98211.0 62 76398.5 63 58315.0 64 44837.5 65 35486.5 66 28386.5 67 22647.0 68 17433.5 69 13178.5 70 10102.0 71 8086.5 72 6694.5 73 5179.0 74 3821.0 75 2809.5 76 1941.5 77 1253.0 78 736.0 79 400.5 80 205.0 81 110.0 82 64.5 83 41.0 84 27.0 85 20.0 86 16.5 87 11.5 88 9.5 89 6.5 90 4.0 91 3.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.02008930099682571 2 0.0 3 0.0 4 0.0 5 0.08410480453200217 6 0.008360031984329264 7 0.0 8 0.0 9 0.0 10-11 0.05046648618126351 12-13 0.0 14-15 0.0 16-17 1.9423291121177584 18-19 9.133686280430787 20-21 1.8017310311054447E-5 22-23 1.8017310311054447E-5 24-25 1.801731031105445E-4 26-27 1.6215579279949005E-4 28-29 0.0013512982733290837 30-31 6.84657791820069E-4 32-33 6.125885505758513E-4 34-35 6.035798954203241E-4 36-37 2.972856201323984E-4 38-39 0.0015404800315951554 40-41 7.657356882198141E-4 42-43 0.001288237687240393 44-45 0.31414982258354535 46-47 0.0017747050656388632 48-49 7.657356882198141E-4 50-51 3.5582386133301434 52-53 3.6034620622108895E-5 54-55 0.8923613450859047 56-57 1.2062048733941622 58-59 0.004657474715407575 60-61 0.00269358789150264 62-63 0.891784791155951 64-65 2.252163788881806E-4 66-67 0.0 68-69 9.909520671079946E-5 70-71 0.0014233675145733015 72-73 0.001333280963018029 74-75 0.0019909127893715166 76-77 0.002603501339947368 78-79 0.0019008262378162441 80-81 5.585366196426878E-4 82-83 0.0020539733754602074 84-85 0.002522423443547623 86-87 1.171125170218539E-4 88-89 4.954760335539974E-4 90-91 4.053894819987251E-4 92-93 0.0 94-95 3.873721716876707E-4 96-97 0.0 98-99 1.3512982733290837E-4 100-101 0.1551560677436454 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5550218.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.6614880431695 #Duplication Level Percentage of deduplicated Percentage of total 1 89.75700591085808 59.833355763173614 2 6.045668841047689 8.060265623209258 3 1.6208400165652588 3.2414282215246693 4 0.7415992206382567 1.977444303176039 5 0.45353003870852693 1.5116493626293335 6 0.2820119551277049 1.127960194486581 7 0.19896172751320634 0.9284159373768965 8 0.12806879938555474 0.6829805391154583 9 0.11213089994896742 0.6727331381596134 >10 0.5921074992587031 7.305432623799598 >50 0.035281162308299974 1.5794822576216425 >100 0.027757453099602678 4.3455420530071445 >500 0.002954360308462691 1.3453010661056144 >1k 0.00196956846254986 1.9560234425588126 >5k 2.8136692321773528E-5 0.12938121806039926 >10k+ 8.441007696532058E-5 5.302604255995285 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 194932 3.512150333554466 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 55408 0.9983031297149049 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA 32370 0.5832203347688325 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA 6898 0.12428340652565358 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 1.8017310311054447E-5 4 1.8017310311054447E-5 0.0 0.0 0.0 3.6034620622108895E-5 5 3.6034620622108895E-5 0.0 0.0 0.0 3.6034620622108895E-5 6 3.6034620622108895E-5 0.0 0.0 0.0 3.6034620622108895E-5 7 3.6034620622108895E-5 0.0 0.0 1.8017310311054447E-5 3.6034620622108895E-5 8 3.6034620622108895E-5 0.0 0.0 1.8017310311054447E-5 7.206924124421779E-5 9 5.405193093316335E-5 0.0 0.0 1.8017310311054447E-5 7.206924124421779E-5 10-11 5.405193093316335E-5 0.0 0.0 1.8017310311054447E-5 1.081038618663267E-4 12-13 9.909520671079946E-5 0.0 0.0 1.8017310311054447E-5 1.2612117217738114E-4 14-15 1.2612117217738114E-4 0.0 0.0 1.8017310311054447E-5 1.2612117217738114E-4 16-17 1.6215579279949005E-4 0.0 0.0 1.8017310311054447E-5 1.2612117217738114E-4 18-19 2.3422503404370784E-4 0.0 0.0 1.8017310311054447E-5 1.3512982733290835E-4 20-21 2.8827696497687116E-4 0.0 0.0 1.8017310311054447E-5 1.6215579279949005E-4 22-23 3.062942752879256E-4 0.0 0.0 1.8017310311054447E-5 1.6215579279949005E-4 24-25 3.783635165321434E-4 0.0 0.0 1.8017310311054447E-5 1.6215579279949005E-4 26-27 4.4142410262083397E-4 0.0 0.0 1.8017310311054447E-5 1.6215579279949005E-4 28-29 5.22501999020579E-4 9.008655155527224E-6 0.0 1.8017310311054447E-5 1.6215579279949005E-4 30-31 6.306058608869057E-4 1.8017310311054447E-5 0.0 1.8017310311054447E-5 1.7116444795501728E-4 32-33 7.20692412442178E-4 1.8017310311054447E-5 0.0 1.8017310311054447E-5 1.891817582660717E-4 34-35 8.01770308841923E-4 1.8017310311054447E-5 0.0 1.8017310311054447E-5 1.9819041342159893E-4 36-37 9.008655155527225E-4 1.8017310311054447E-5 0.0 1.8017310311054447E-5 1.9819041342159893E-4 38-39 0.001117073239285376 1.8017310311054447E-5 0.0 1.8017310311054447E-5 2.0719906857712616E-4 40-41 0.0012251771011517025 1.8017310311054447E-5 0.0 1.8017310311054447E-5 2.162077237326534E-4 42-43 0.001324272307862502 1.8017310311054447E-5 0.0 1.8017310311054447E-5 2.3422503404370784E-4 44-45 0.0014233675145733015 1.8017310311054447E-5 0.0 1.8017310311054447E-5 2.4323368919923504E-4 46-47 0.001522462721284101 1.8017310311054447E-5 0.0 1.8017310311054447E-5 2.522423443547623E-4 48-49 0.0016575925486170093 1.8017310311054447E-5 0.0 1.8017310311054447E-5 2.79268309821344E-4 50-51 0.001810739686260972 2.702596546658167E-5 0.0 3.6034620622108895E-5 2.8827696497687116E-4 52-53 0.002053973375460207 3.6034620622108895E-5 0.0 3.6034620622108895E-5 2.8827696497687116E-4 54-55 0.0022611724440373335 3.6034620622108895E-5 0.0 3.6034620622108895E-5 2.972856201323984E-4 56-57 0.0025044061332365683 3.6034620622108895E-5 0.0 3.6034620622108895E-5 3.062942752879256E-4 58-59 0.0027386311672802765 3.6034620622108895E-5 0.0 3.6034620622108895E-5 3.243115855989801E-4 60-61 0.002882769649768712 3.6034620622108895E-5 0.0 3.6034620622108895E-5 3.243115855989801E-4 62-63 0.0031169946838124196 3.6034620622108895E-5 0.0 3.6034620622108895E-5 3.333202407545073E-4 64-65 0.0033151850972340186 3.6034620622108895E-5 0.0 3.6034620622108895E-5 3.423288959100345E-4 66-67 0.0034773408900335085 3.6034620622108895E-5 0.0 3.6034620622108895E-5 3.423288959100345E-4 68-69 0.003846695751410125 3.6034620622108895E-5 0.0 3.6034620622108895E-5 3.423288959100345E-4 70-71 0.004189024647320159 3.6034620622108895E-5 0.0 3.6034620622108895E-5 3.60346206221089E-4 72-73 0.004549370853541249 3.6034620622108895E-5 0.0 3.6034620622108895E-5 3.7836351653214344E-4 74-75 0.004882691094295756 3.6034620622108895E-5 0.0 3.6034620622108895E-5 3.9638082684319786E-4 76-77 0.005234028645361318 3.6034620622108895E-5 0.0 3.6034620622108895E-5 3.9638082684319786E-4 78-79 0.00587364316140375 3.6034620622108895E-5 0.0 3.6034620622108895E-5 4.143981371542523E-4 80-81 0.006819551952734109 3.6034620622108895E-5 0.0 3.6034620622108895E-5 4.143981371542523E-4 82-83 0.007576278985798395 3.6034620622108895E-5 0.0 3.6034620622108895E-5 4.2340679230977956E-4 84-85 0.008810464742105626 3.6034620622108895E-5 0.0 5.405193093316335E-5 4.504327577763612E-4 86-87 0.009864477395302312 3.6034620622108895E-5 0.0 5.405193093316335E-5 4.504327577763612E-4 88-89 0.011296853565031139 3.6034620622108895E-5 0.0 5.405193093316335E-5 4.504327577763612E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTATC 224210 0.0 57.522472 7 GTATCAA 225330 0.0 57.42002 9 GTGGTAT 224780 0.0 57.325985 6 GGTATCA 226410 0.0 56.981995 8 AGTGGTA 233575 0.0 55.102276 5 CAGTGGT 236355 0.0 55.058983 4 AAGCAGT 241205 0.0 54.031513 1 AGCAGTG 245940 0.0 52.856518 2 GCAGTGG 250110 0.0 52.06812 3 AGTACTT 32140 0.0 42.566257 20-21 GTACTTT 31705 0.0 42.32423 22-23 TAACGCC 9180 0.0 41.829784 54-55 CTTAACG 9220 0.0 41.69969 52-53 AGAGTAC 44950 0.0 41.567432 18-19 GAGTACT 34225 0.0 41.36294 20-21 GTGGTCT 3700 0.0 40.679657 6 AACGCCC 9545 0.0 40.50423 54-55 TACTTTT 33355 0.0 40.27924 22-23 GGTCTAT 8795 0.0 40.102676 36-37 CTATACC 7870 0.0 39.876217 38-39 >>END_MODULE