FastQCFastQC Report
Sat 18 Jun 2016
SRR799684_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR799684_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8000000
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT632430.7905374999999999No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA475110.5938875No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA368400.46049999999999996No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA93550.1169375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3625250.062.351436
TGGTATC3607550.062.3480457
AGTGGTA3681750.061.470815
CAGTGGT3677050.061.386374
GTATCAA3697950.061.0823639
GGTATCA3702100.060.926278
AAGCAGT3870150.059.155591
AGCAGTG3963300.057.6040952
GCAGTGG4000150.056.938423
AGTACTT116800.050.5823520-21
GTACTTT113700.050.23977322-23
GTCACCG111100.049.5922622-23
TCACCGG111550.048.9079622-23
GAGTACT142850.048.1882120-21
GAGTCAC158900.043.0659120-21
TACTTTT135250.041.9852222-23
TAACGCC148950.041.7615254-55
CTTAACG149600.041.627952-53
AGTCACC133800.041.5314920-21
CTATTCG148400.041.33355390-91
Produced by FastQC (version 0.11.5)