##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR799684_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8000000 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.38762325 31.0 27.0 31.0 16.0 34.0 2 28.68696675 31.0 27.0 33.0 18.0 34.0 3 28.312656125 31.0 27.0 33.0 16.0 34.0 4 31.002755 35.0 30.0 37.0 16.0 37.0 5 31.859405125 35.0 31.0 37.0 18.0 37.0 6 31.3621645 35.0 30.0 37.0 17.0 37.0 7 31.257859375 35.0 30.0 37.0 17.0 37.0 8 31.14149075 35.0 30.0 37.0 16.0 37.0 9 32.249164125 37.0 30.0 39.0 15.0 39.0 10-11 32.5326563125 37.0 30.5 39.0 16.5 39.0 12-13 32.34070475 37.0 30.0 39.0 16.0 39.0 14-15 33.778889 38.0 31.0 40.0 17.0 41.0 16-17 33.208568812500005 38.0 30.0 40.0 16.5 41.0 18-19 33.273898875 38.0 30.5 40.0 17.0 41.0 20-21 33.277698125 38.0 30.0 40.0 17.0 41.0 22-23 27.81637 33.0 16.0 39.5 9.5 40.5 24-25 28.178001625 31.0 20.5 38.0 11.5 40.0 26-27 30.300250499999997 34.0 25.0 38.5 10.0 40.0 28-29 31.10288525 35.0 26.5 39.0 10.0 40.5 30-31 30.5648753125 34.5 25.5 39.5 9.5 40.5 32-33 30.0421750625 34.0 24.5 39.0 9.0 40.0 34-35 30.2364175 34.0 25.0 39.0 9.0 40.0 36-37 30.2429053125 34.0 24.5 39.0 9.0 40.0 38-39 30.1489876875 34.0 25.0 39.0 9.0 40.0 40-41 29.8522043125 33.0 24.0 38.0 9.0 40.0 42-43 29.996388125000003 34.0 24.0 38.5 9.0 40.0 44-45 29.9468040625 33.0 24.0 38.0 9.0 40.0 46-47 29.640431812499997 33.0 24.0 38.0 9.0 40.0 48-49 29.3085924375 33.0 23.5 38.0 8.5 40.0 50-51 28.9745091875 32.5 23.0 38.0 8.0 40.0 52-53 28.736385249999998 32.5 22.5 37.5 8.0 40.0 54-55 28.5117465 32.0 22.0 37.0 8.0 40.0 56-57 28.3439720625 32.0 22.0 37.0 8.0 40.0 58-59 28.232578750000002 32.0 22.0 36.5 8.0 40.0 60-61 27.789129250000002 31.0 21.5 36.0 8.0 39.0 62-63 27.516945125 31.0 21.0 35.5 8.0 39.0 64-65 27.2451538125 31.0 21.0 35.0 8.0 39.0 66-67 27.0146823125 30.5 20.5 35.0 7.5 38.5 68-69 26.8783386875 30.0 20.0 35.0 7.5 38.0 70-71 26.308114562500002 30.0 19.5 35.0 7.0 37.0 72-73 26.266064 30.0 20.0 34.5 7.0 37.0 74-75 26.180834375 29.5 20.0 34.0 7.0 36.0 76-77 25.83531825 29.0 19.0 34.0 7.0 36.0 78-79 25.5117194375 29.0 18.5 34.0 7.0 35.5 80-81 25.3404144375 29.0 18.5 34.0 7.0 35.0 82-83 25.159777812500003 29.0 18.0 34.0 7.0 35.0 84-85 24.935032562499998 29.0 18.0 34.0 7.0 35.0 86-87 24.822046375 29.0 18.0 34.0 7.0 35.0 88-89 24.69378975 28.0 18.0 34.0 7.0 35.0 90-91 24.631392875000003 28.0 17.5 34.0 7.0 35.0 92-93 24.46124125 28.0 17.0 34.0 7.0 35.0 94-95 24.356401062499998 28.0 17.0 34.0 7.0 35.0 96-97 24.210629812500002 27.5 17.0 34.0 7.0 35.0 98-99 24.0989030625 27.0 17.0 33.0 7.0 35.0 100-101 22.8225286875 26.0 12.5 32.5 7.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40644570745448405 1101 2 0.3803177291005646 1101 3 0.5342605251723107 1101 4 0.44942454065570914 1101 5 0.4075848509676021 1101 6 0.4241742764090972 1101 7 0.4332357096321644 1101 8 0.5330347371714304 1101 9 0.6047336688588558 1101 10-11 0.6259738680480069 1101 12-13 0.6158026179799592 1101 14-15 0.5896061886121728 1101 16-17 0.5868584419456084 1101 18-19 0.7301223845891016 1101 20-21 0.7344050773273096 1101 22-23 6.553788887703007 1101 24-25 5.353349103646778 1101 26-27 1.790521936965085 1101 28-29 1.0890236169115646 1101 30-31 0.8909219427129322 1101 32-33 0.8956084835730636 1101 34-35 0.7670228567300583 1101 36-37 0.8051270508739883 1101 38-39 0.7836018187937519 1101 40-41 0.8604278111901671 1101 42-43 0.7884247034106373 1101 44-45 0.6839575493861254 1101 46-47 0.6231802096031274 1101 48-49 0.6588168746093004 1101 50-51 0.7616852122880893 1101 52-53 0.6292554335927925 1101 54-55 0.5826421000218645 1101 56-57 0.5520479237770424 1101 58-59 0.5856999592639092 1101 60-61 0.4947113888362331 1101 62-63 0.45819286099866474 1101 64-65 0.5166114185586466 1101 66-67 0.4388324173749325 1101 68-69 0.4087454982719123 1101 70-71 0.39291282956567386 1101 72-73 0.3461567192601862 1101 74-75 0.42427911747496694 1101 76-77 0.5472047301295859 1101 78-79 0.4485360146672477 1101 80-81 0.353341568527874 1101 82-83 0.2588231383078252 1101 84-85 0.2632583324369122 1101 86-87 0.2909994369316742 1101 88-89 0.4013582329815186 1101 90-91 0.2836960140947866 1101 92-93 0.19130981935323987 1101 94-95 0.15920945112958762 1101 96-97 0.18686415535510648 1101 98-99 0.2459823839108175 1101 100-101 0.24411742238389067 1102 1 0.3461223369350286 1102 2 0.2656269052219642 1102 3 0.4511817684824564 1102 4 0.21108108603382902 1102 5 0.3346776348848337 1102 6 0.21026236191195835 1102 7 0.37650358167568854 1102 8 0.3891762994083692 1102 9 0.4115068285236916 1102 10-11 0.3285306509687871 1102 12-13 0.4758916349436575 1102 14-15 0.5052299895957617 1102 16-17 0.5042761953871491 1102 18-19 0.5849778212946646 1102 20-21 0.5653913747404005 1102 22-23 5.497764817212719 1102 24-25 4.715334266383948 1102 26-27 1.5429559621751991 1102 28-29 0.7687504147861723 1102 30-31 0.7277262553442121 1102 32-33 0.7196811926945941 1102 34-35 0.6884691209989491 1102 36-37 0.7005509354472998 1102 38-39 0.6464669177850446 1102 40-41 0.842901717797421 1102 42-43 0.751527920071748 1102 44-45 0.6601295795780473 1102 46-47 0.595239654568438 1102 48-49 0.5940465318821069 1102 50-51 0.7589563390794929 1102 52-53 0.5898060309064341 1102 54-55 0.5616510453758963 1102 56-57 0.5023814496123791 1102 58-59 0.5423138502057938 1102 60-61 0.48323145042733984 1102 62-63 0.480316139878628 1102 64-65 0.4044402700457077 1102 66-67 0.3437202048041925 1102 68-69 0.3536658760639284 1102 70-71 0.4163418355823296 1102 72-73 0.31382547306882813 1102 74-75 0.2698020850927918 1102 76-77 0.30442842032446293 1102 78-79 0.3574724327860075 1102 80-81 0.3137061964893917 1102 82-83 0.2837277310547357 1102 84-85 0.19262915172827988 1102 86-87 0.1499669798412242 1102 88-89 0.1858434057188596 1102 90-91 0.21174870275728352 1102 92-93 0.1727624023044818 1102 94-95 0.13412156425778576 1102 96-97 0.0970635197853511 1102 98-99 0.12795788564694988 1102 100-101 0.08809938520621685 1103 1 0.061139419524096184 1103 2 -0.05327353494900322 1103 3 0.05867374646997803 1103 4 -0.04738124759085949 1103 5 -0.04681691432866586 1103 6 -0.09114869899226008 1103 7 -0.016310478841983667 1103 8 0.03183424317618844 1103 9 0.16515097267407697 1103 10-11 -0.025036733011816636 1103 12-13 0.10637092708423523 1103 14-15 0.11319119148592449 1103 16-17 0.10787355794260378 1103 18-19 0.17041092563558635 1103 20-21 0.11665376299656316 1103 22-23 -9.207964885559242 1103 24-25 -7.809303681674443 1103 26-27 -1.6228026034949785 1103 28-29 -0.3048495929886208 1103 30-31 0.029544604646478234 1103 32-33 0.18048711584870247 1103 34-35 0.17297694153691978 1103 36-37 0.2511192099475359 1103 38-39 0.232681205157931 1103 40-41 0.2808109556692706 1103 42-43 0.24333637557788634 1103 44-45 0.17385404840275598 1103 46-47 0.14621813445714338 1103 48-49 0.10598754987364245 1103 50-51 0.2853477882773028 1103 52-53 0.15042746019246422 1103 54-55 0.12694486515695047 1103 56-57 0.05561056811982468 1103 58-59 0.015757468304396838 1103 60-61 0.12416320012945903 1103 62-63 0.05311220631993052 1103 64-65 -0.009393565703124551 1103 66-67 -0.09959294555470777 1103 68-69 -0.06035121022192058 1103 70-71 -0.015231612445909093 1103 72-73 -0.06211601447152404 1103 74-75 -0.13612797015558442 1103 76-77 0.049644021729335464 1103 78-79 0.0037267513758898474 1103 80-81 -0.025395160494554148 1103 82-83 -0.0011104704179238922 1103 84-85 -0.07366090403764503 1103 86-87 -0.04365929933127788 1103 88-89 0.05558344382110647 1103 90-91 -0.06865134832759168 1103 92-93 -0.06439364842319151 1103 94-95 -0.05891482580425489 1103 96-97 -0.12647310987423666 1103 98-99 -0.09244543420006934 1103 100-101 -0.04659729746520114 1104 1 -1.1926424488962724 1104 2 -1.1512984546122524 1104 3 -0.7676988130693658 1104 4 -1.0316680466744224 1104 5 -1.0918282628636433 1104 6 -0.45040779048465396 1104 7 -0.35577352575290533 1104 8 -0.14161581360594866 1104 9 -0.28289945301250086 1104 10-11 -0.3790909425212021 1104 12-13 -0.5863447095986096 1104 14-15 -1.1572013927312241 1104 16-17 -1.332164681511319 1104 18-19 -1.238962673442849 1104 20-21 -1.1481590603312597 1104 22-23 -9.964838149451388 1104 24-25 -8.210646620931453 1104 26-27 -3.2259629221116555 1104 28-29 -1.8701853754154065 1104 30-31 -1.5530932953237517 1104 32-33 -1.6618442512023748 1104 34-35 -1.525337921706928 1104 36-37 -1.6139922865459049 1104 38-39 -1.6074562236008383 1104 40-41 -1.5116390953948944 1104 42-43 -1.63115184179782 1104 44-45 -1.5317377131531984 1104 46-47 -1.4491544096853701 1104 48-49 -1.7020620741801729 1104 50-51 -1.8209838433937406 1104 52-53 -1.5316535801078288 1104 54-55 -1.5664529225182804 1104 56-57 -1.5590299514406567 1104 58-59 -1.5188374024877653 1104 60-61 -1.4408158586942612 1104 62-63 -1.4899181816000429 1104 64-65 -1.469189785769732 1104 66-67 -1.5173469443896117 1104 68-69 -1.6558156402266988 1104 70-71 -1.5885569437465037 1104 72-73 -1.6989751091862324 1104 74-75 -1.6566217133251016 1104 76-77 -1.454164599425681 1104 78-79 -1.4093797262129506 1104 80-81 -1.6346206272634198 1104 82-83 -1.6024371983329964 1104 84-85 -1.6754346406839105 1104 86-87 -1.5832383890439559 1104 88-89 -1.6851004007479915 1104 90-91 -1.6288086608846797 1104 92-93 -1.6976435343690248 1104 94-95 -1.6855548132955924 1104 96-97 -1.7978272342424368 1104 98-99 -1.7744942829922827 1104 100-101 -1.6977179505897446 1105 1 0.5486524928207395 1105 2 0.5603814088702528 1105 3 0.6159162848345723 1105 4 0.5346884076193028 1105 5 0.6310372551234806 1105 6 0.6165706312341008 1105 7 0.27715485592366207 1105 8 0.5768386567991577 1105 9 0.4977830884811141 1105 10-11 0.36781010493203325 1105 12-13 0.48813829020134136 1105 14-15 0.8651965542122042 1105 16-17 0.5267281140226885 1105 18-19 0.6461849558236494 1105 20-21 0.8477746414724479 1105 22-23 4.003293359616894 1105 24-25 4.018789560499577 1105 26-27 1.3266088735443091 1105 28-29 0.5894656301863144 1105 30-31 0.4618196673988919 1105 32-33 0.13452275991787843 1105 34-35 0.3821317188478588 1105 36-37 0.6013935748072718 1105 38-39 0.4870813514157142 1105 40-41 0.5865698292857324 1105 42-43 0.6022585628788732 1105 44-45 0.5861069369407765 1105 46-47 0.440556780806606 1105 48-49 0.3836231350472268 1105 50-51 0.5006613635696233 1105 52-53 0.5967824205738843 1105 54-55 0.6366406792312951 1105 56-57 0.6513780222047281 1105 58-59 0.7348049423253791 1105 60-61 0.7049251858022991 1105 62-63 0.6654181490649513 1105 64-65 0.6116371068781632 1105 66-67 0.5285077250653671 1105 68-69 0.561531620530566 1105 70-71 0.5341643914963825 1105 72-73 0.6135096063881562 1105 74-75 0.5412800713862609 1105 76-77 0.5901888186725301 1105 78-79 0.6649450437181521 1105 80-81 0.5483080798979394 1105 82-83 0.447208602426894 1105 84-85 0.49617981039592607 1105 86-87 0.4834487804655119 1105 88-89 0.5091312811813182 1105 90-91 0.4332599064641727 1105 92-93 0.4539986195146355 1105 94-95 0.38197787717127696 1105 96-97 0.3907029365835335 1105 98-99 0.3132653384919024 1105 100-101 0.2766743852334379 1106 1 -0.2835210983619305 1106 2 -0.35538774610705914 1106 3 -0.5776172085053517 1106 4 -0.4461215012139448 1106 5 -0.2939911813657936 1106 6 -0.29084055913334694 1106 7 -0.15966267834741998 1106 8 -0.2787085592956764 1106 9 -0.3183799068383806 1106 10-11 -0.23746920991941778 1106 12-13 -0.6628653361691548 1106 14-15 -0.8273560474630557 1106 16-17 -0.4836907066220988 1106 18-19 -1.599899930192862 1106 20-21 -1.4502631159389452 1106 22-23 4.122316359420118 1106 24-25 3.7180371198712407 1106 26-27 0.5645282860409715 1106 28-29 -1.0781306743710424 1106 30-31 -0.42665475965650757 1106 32-33 -0.2705915359980935 1106 34-35 -0.305569722173864 1106 36-37 -0.21862756604390654 1106 38-39 -0.6827574854514076 1106 40-41 -1.4368905199091202 1106 42-43 -0.7364714153509198 1106 44-45 -1.0155467783400027 1106 46-47 -0.6965281153388503 1106 48-49 -0.4115080872890573 1106 50-51 -1.7439524112873492 1106 52-53 -1.2410396509044794 1106 54-55 -0.7218423696076499 1106 56-57 -1.1245222831747057 1106 58-59 -0.9550830556956278 1106 60-61 -0.5983106348803666 1106 62-63 -0.4088693705275048 1106 64-65 -0.32707634789429463 1106 66-67 -0.31743940566540374 1106 68-69 -0.3662532248286112 1106 70-71 -1.0928837855257818 1106 72-73 -0.7529908995048622 1106 74-75 -0.7617869235020933 1106 76-77 -0.9250676784003851 1106 78-79 -0.4022192904546138 1106 80-81 -0.6071605315303827 1106 82-83 -0.4623055619466889 1106 84-85 -0.48922103411658213 1106 86-87 -0.4908935725992585 1106 88-89 -0.9629029099418887 1106 90-91 -0.5624450673313248 1106 92-93 -0.44289549780424053 1106 94-95 -0.4006620470857314 1106 96-97 -0.33170292807729496 1106 98-99 -0.406576726358864 1106 100-101 -0.36336904050187613 1107 1 0.1488362793313449 1107 2 0.09991478200286963 1107 3 0.2544695353170958 1107 4 0.1743157169079943 1107 5 0.16580298995153697 1107 6 -0.04766046527946344 1107 7 -0.24968845008326213 1107 8 -0.26514780025992835 1107 9 -0.10315332056607218 1107 10-11 0.2161120778746266 1107 12-13 -0.3682190119498756 1107 14-15 0.16325907905414283 1107 16-17 0.24394335546411128 1107 18-19 0.31986509467529345 1107 20-21 -0.15903856679062756 1107 22-23 4.483263642120992 1107 24-25 4.136980995362247 1107 26-27 0.8959090331515256 1107 28-29 0.07335814795787243 1107 30-31 0.1941186219183315 1107 32-33 -0.12353314711850416 1107 34-35 0.030709856186113882 1107 36-37 -0.20469106346621047 1107 38-39 -0.07818476511417671 1107 40-41 0.1268796985617051 1107 42-43 0.2213646804645144 1107 44-45 0.16155347665085174 1107 46-47 0.1372146771660745 1107 48-49 0.0637881566941445 1107 50-51 0.1870435659970795 1107 52-53 0.13029081708243595 1107 54-55 0.08469644535226095 1107 56-57 0.006720054865638758 1107 58-59 0.04572022822598498 1107 60-61 0.14767637368571584 1107 62-63 0.05542814809494345 1107 64-65 -0.005085832757625752 1107 66-67 -0.06346753704663399 1107 68-69 -0.06183369165561459 1107 70-71 0.01902063652100594 1107 72-73 -0.04711951310446594 1107 74-75 -0.15899417685794504 1107 76-77 -0.005399231431624685 1107 78-79 0.10121987083394757 1107 80-81 0.06840807616256228 1107 82-83 -0.0038507422746612008 1107 84-85 -0.08336414705030393 1107 86-87 -0.021191586612442137 1107 88-89 0.06665947861738886 1107 90-91 0.03777661839898627 1107 92-93 -0.027301531742036644 1107 94-95 -0.07530298761098564 1107 96-97 -0.11951794945235505 1107 98-99 -0.009774123566096193 1107 100-101 -0.06964142590143396 1108 1 0.5723369245390266 1108 2 0.542915080314252 1108 3 0.6666598672103703 1108 4 0.6171479114108998 1108 5 0.7731862209650373 1108 6 0.729558529284855 1108 7 0.7543068697318063 1108 8 0.7533412987425265 1108 9 0.6785587827138002 1108 10-11 0.49651354839427597 1108 12-13 0.7291280269452685 1108 14-15 0.8493349458576205 1108 16-17 0.5785315035625942 1108 18-19 0.8456869874755952 1108 20-21 0.7712968768285293 1108 22-23 6.135243034100206 1108 24-25 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2112 74-75 0.5135091025949237 2112 76-77 0.667652045321212 2112 78-79 0.6324252963615571 2112 80-81 0.618342741370526 2112 82-83 0.5938882224369237 2112 84-85 0.6312276799282373 2112 86-87 0.6271541677474524 2112 88-89 0.6485028087036255 2112 90-91 0.5140320756918442 2112 92-93 0.4994662393132465 2112 94-95 0.43684883059344415 2112 96-97 0.4025818059606543 2112 98-99 0.35374559508861125 2112 100-101 0.33088941826515494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 207.0 3 286.0 4 262.0 5 251.0 6 285.0 7 658.0 8 2565.0 9 11982.0 10 41405.0 11 92012.0 12 139725.0 13 161683.0 14 164984.0 15 165477.0 16 167930.0 17 171803.0 18 175625.0 19 181699.0 20 188580.0 21 198543.0 22 211293.0 23 228105.0 24 245875.0 25 255925.0 26 258788.0 27 261091.0 28 277063.0 29 298183.0 30 319939.0 31 352944.0 32 399568.0 33 459674.0 34 540706.0 35 627104.0 36 636803.0 37 510148.0 38 224426.0 39 26380.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.171623242661116 52.34286406475521 19.422772694270524 16.062739998313145 2 16.05590998251266 53.70360437774908 14.841348041854097 15.399137597884167 3 48.11203548543864 20.743573371568015 15.48792165557705 15.656469487416299 4 15.432593953309606 20.65422416360568 15.831895675423313 48.0812862076614 5 15.31898023244867 53.58439320009937 16.27595781212043 14.820668755331528 6 47.4718890432899 21.34716325231531 15.699594918201079 15.481352786193717 7 15.57736562457215 21.57740188438496 48.17332440591735 14.671908085125537 8 46.50671099239577 22.486593892260576 15.854624210179166 15.152070905164491 9 46.28989703720162 22.025326395209248 16.34945008860625 15.335326478982884 10-11 14.424605555972295 37.99831220463581 32.37391543520116 15.203166804190735 12-13 14.382016262650964 22.167651637792684 32.07963312763715 31.370698971919197 14-15 14.57019177645114 53.69201143789767 16.262859101839222 15.474937683811968 16-17 30.69438406700826 21.299065288523295 16.25436639824703 31.752184246221415 18-19 14.818898500123204 37.13227402365113 16.474159995609373 31.57466748061629 20-21 31.08419001500029 36.93464041747284 16.553542557304944 15.427627010221926 22-23 34.062126449778695 19.94941576115549 29.183347525710033 16.805110263355775 24-25 24.551988561963576 29.76613407725927 21.60952942915624 24.072347931620914 26-27 26.8507936154659 25.610807547022596 22.483778245177767 25.05462059233373 28-29 45.984651197829876 19.83468981834448 18.361058517816232 15.819600466009415 30-31 35.63001602527231 26.305360713674798 20.65263846805599 17.411984792996897 32-33 25.197373986152698 30.133882141976727 24.428078244244144 20.240665627626424 34-35 22.136275470571594 28.334694681364848 23.79365027266035 25.735379575403204 36-37 26.871517474581726 25.131842704507797 26.76068682990847 21.235952991002012 38-39 28.375153757535948 24.493048727618486 24.363402849183256 22.76839466566231 40-41 21.566489011794356 29.341630619602633 27.138755642176342 21.95312472642667 42-43 21.663119950259468 25.979573139637242 27.563242306816615 24.794064603286674 44-45 21.412635481565328 32.09768147312553 23.197836709374194 23.29184633593494 46-47 25.355955077541147 24.66468879102351 23.685659109130455 26.293697022304883 48-49 22.663062573116164 28.017107728684348 23.81914383057475 25.500685867624735 50-51 25.25980763828453 29.559235065876223 23.33066212924692 21.85029516659233 52-53 26.064212227750993 24.615525154925724 26.732429167722575 22.587833449600705 54-55 24.24573652022233 27.224206996793487 25.254050902013 23.276005580971187 56-57 24.978404734717373 26.41513845287506 23.828951495952694 24.777505316454874 58-59 29.853325589057818 24.43816385644914 23.598099609806052 22.110410944686993 60-61 26.136785259410296 26.755803491317348 23.461648843678546 23.64576240559381 62-63 25.771557877572338 28.169215145669458 23.59762267712258 22.461604299635628 64-65 22.790453051822393 26.566694570421905 25.98377930886844 24.659073068887263 66-67 25.92009784835156 26.094936007708235 26.050773211538043 21.934192932402162 68-69 28.97697662578246 25.411421938235573 23.817798034277118 21.793803401704853 70-71 22.54992912342014 27.47357763484319 27.122742158834924 22.853751082901752 72-73 23.156369705977504 25.350824960264063 26.047039223316755 25.44576611044168 74-75 23.266222693779486 29.978566121944677 24.422699380826863 22.332511803448977 76-77 25.364686297211563 24.51310255297598 24.95040856441936 25.171802585393095 78-79 23.130853813926585 26.70391553173152 23.773272648427064 26.391958005914834 80-81 25.926782881675166 26.62378660154155 24.91558389636264 22.533846620420647 82-83 26.03257299748633 25.290699946043212 26.179733564355836 22.496993492114626 84-85 27.095185651608762 25.60560827373653 24.03152247308939 23.267683601565324 86-87 24.76308801645587 25.790217148584205 24.536272158025174 24.910422676934747 88-89 28.39361781803537 24.45575543062999 23.467631028370324 23.68299572296432 90-91 25.854815842374624 26.566908954684344 23.48513938973574 24.093135813205297 92-93 24.71219240495884 28.30776116725225 25.15898564001679 21.82106078777212 94-95 22.8052603507063 27.00848666063565 25.912289609442844 24.27396337921521 96-97 26.115476507499523 24.962586781788108 25.374563512454195 23.547373198258175 98-99 28.444154643341985 24.98088698517628 24.286054110353163 22.288904261128575 100-101 23.69593573681744 28.2415551041986 25.511921558585236 22.55058760039872 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 40188.0 1 28277.5 2 12879.5 3 8566.0 4 7229.0 5 6507.0 6 6276.5 7 6299.0 8 6422.0 9 6607.0 10 7046.0 11 8814.5 12 38236.5 13 40267.5 14 12575.0 15 10512.0 16 10323.5 17 10780.5 18 11705.5 19 12679.0 20 13952.5 21 15859.0 22 18070.0 23 20952.5 24 24614.0 25 28220.0 26 32090.5 27 36778.5 28 41953.0 29 48289.0 30 55583.5 31 63310.5 32 71695.0 33 81209.5 34 92129.5 35 104183.5 36 116090.5 37 125270.0 38 131212.0 39 136267.0 40 142703.0 41 150085.5 42 158274.5 43 167551.0 44 178874.0 45 191352.0 46 204160.0 47 218749.0 48 232982.0 49 245478.5 50 263255.5 51 292047.0 52 334609.0 53 403038.5 54 427586.5 55 404789.5 56 393532.5 57 366965.0 58 321818.0 59 273999.5 60 229481.0 61 185749.5 62 145800.0 63 114008.5 64 90190.0 65 71711.0 66 57224.5 67 45963.5 68 36161.5 69 27936.0 70 21918.0 71 17516.0 72 14097.0 73 10981.5 74 8283.0 75 6248.0 76 4571.5 77 3252.5 78 2363.5 79 1638.0 80 1067.0 81 702.5 82 486.0 83 329.5 84 215.0 85 149.5 86 101.5 87 61.0 88 36.5 89 23.5 90 10.0 91 9.0 92 8.5 93 4.5 94 3.5 95 2.0 96 2.5 97 3.5 98 1.5 99 2.5 100 5.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.109975 2 0.0205375 3 0.020962500000000002 4 0.025474999999999998 5 0.019862499999999998 6 0.00785 7 0.027062499999999996 8 0.012924999999999999 9 0.028212499999999998 10-11 0.017499999999999998 12-13 0.02245 14-15 0.020962500000000002 16-17 0.0212875 18-19 0.014287499999999998 20-21 0.0102625 22-23 15.653075 24-25 0.052775 26-27 0.02911875 28-29 0.02884375 30-31 0.0329 32-33 0.03194375 34-35 0.030156250000000002 36-37 0.02765 38-39 0.0353875 40-41 0.04965 42-43 0.05133125 44-45 0.04348125 46-47 0.061125000000000006 48-49 0.06271249999999999 50-51 0.060675 52-53 0.04181875 54-55 0.02188125 56-57 0.0214875 58-59 0.0336875 60-61 0.0413125 62-63 0.04618125 64-65 0.03585625 66-67 0.028975 68-69 0.026600000000000002 70-71 0.01545625 72-73 0.02359375 74-75 0.023925 76-77 0.0142375 78-79 0.02044375 80-81 0.02833125 82-83 0.024168750000000003 84-85 0.0173625 86-87 0.023525 88-89 0.027137500000000002 90-91 0.01483125 92-93 0.01946875 94-95 0.00999375 96-97 0.018625 98-99 0.027218750000000003 100-101 0.029849999999999998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8000000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.06780988558758 #Duplication Level Percentage of deduplicated Percentage of total 1 87.9390718946907 57.22002811558753 2 6.690342852205923 8.706519135534696 3 1.7716156802237353 3.458254568133718 4 0.820992603434453 2.1368076255098636 5 0.5485115737939559 1.7845223401834782 6 0.3822764322426256 1.4924334130142318 7 0.2867940078754243 1.3062740586534671 8 0.2442048843423948 1.2711901590018277 9 0.19316349970141827 1.1311853287865974 >10 1.0639734227522246 11.728688348165823 >50 0.03211382135214432 1.4204505320000973 >100 0.02298718773214838 2.9737755690256016 >500 0.0019454873733196782 0.8512558669637975 >1k 0.0019042720627084038 2.3365493865554288 >5k 4.095208675768165E-5 0.215624497233275 >10k+ 6.142813013652247E-5 1.9664410556505185 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 63243 0.7905374999999999 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA 47511 0.5938875 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36840 0.46049999999999996 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA 9355 0.1169375 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.25E-5 0.0 0.0 0.0 1.25E-5 2 3.75E-5 0.0 0.0 0.0 2.5E-5 3 3.75E-5 0.0 0.0 0.0 2.5E-5 4 3.75E-5 0.0 0.0 0.0 2.5E-5 5 3.75E-5 0.0 0.0 0.0 2.5E-5 6 3.75E-5 0.0 0.0 0.0 2.5E-5 7 3.75E-5 1.25E-5 0.0 0.0 2.5E-5 8 3.75E-5 1.25E-5 0.0 0.0 3.75E-5 9 3.75E-5 1.25E-5 0.0 0.0 3.75E-5 10-11 4.375E-5 1.25E-5 0.0 0.0 3.75E-5 12-13 6.25E-5 1.8750000000000002E-5 0.0 0.0 3.75E-5 14-15 6.25E-5 2.5E-5 0.0 0.0 3.75E-5 16-17 7.500000000000001E-5 2.5E-5 0.0 0.0 3.75E-5 18-19 1.125E-4 2.5E-5 0.0 0.0 3.75E-5 20-21 1.3749999999999998E-4 2.5E-5 0.0 0.0 3.75E-5 22-23 1.5E-4 2.5E-5 0.0 0.0 6.25E-5 24-25 2.625E-4 2.5E-5 0.0 0.0 6.25E-5 26-27 3.5E-4 2.5E-5 0.0 0.0 6.874999999999999E-5 28-29 4.2500000000000003E-4 2.5E-5 0.0 0.0 7.5E-5 30-31 5.6875E-4 2.5E-5 0.0 0.0 7.5E-5 32-33 7.062500000000001E-4 2.5E-5 0.0 0.0 1.0E-4 34-35 8.500000000000001E-4 2.5E-5 0.0 0.0 1.1875E-4 36-37 0.0010187500000000001 2.5E-5 0.0 0.0 1.3125000000000002E-4 38-39 0.00118125 2.5E-5 0.0 0.0 1.4375E-4 40-41 0.00133125 2.5E-5 0.0 0.0 1.5624999999999998E-4 42-43 0.0015687499999999998 2.5E-5 0.0 0.0 1.6875E-4 44-45 0.0016875 2.5E-5 0.0 0.0 1.75E-4 46-47 0.00190625 2.5E-5 0.0 6.25E-6 1.75E-4 48-49 0.0021375 2.5E-5 0.0 1.25E-5 1.9375000000000002E-4 50-51 0.00233125 3.7500000000000003E-5 0.0 5.0E-5 2.0E-4 52-53 0.00248125 6.25E-5 0.0 5.0E-5 2.0E-4 54-55 0.002675 7.5E-5 0.0 6.25E-5 2.0625E-4 56-57 0.0028062499999999997 7.5E-5 0.0 6.25E-5 2.125E-4 58-59 0.00305625 7.5E-5 0.0 6.25E-5 2.125E-4 60-61 0.00331875 7.5E-5 0.0 6.25E-5 2.125E-4 62-63 0.0036437500000000003 7.5E-5 0.0 6.25E-5 2.125E-4 64-65 0.00395625 7.5E-5 0.0 6.874999999999999E-5 2.125E-4 66-67 0.004306249999999999 7.5E-5 0.0 7.5E-5 2.25E-4 68-69 0.00478125 7.5E-5 0.0 7.5E-5 2.25E-4 70-71 0.005156249999999999 7.5E-5 0.0 7.5E-5 2.25E-4 72-73 0.00544375 7.5E-5 0.0 7.5E-5 2.3125E-4 74-75 0.0058625 7.5E-5 0.0 7.5E-5 2.375E-4 76-77 0.00645 7.5E-5 0.0 7.5E-5 2.375E-4 78-79 0.006999999999999999 7.5E-5 0.0 7.5E-5 2.375E-4 80-81 0.00763125 7.5E-5 0.0 7.5E-5 2.375E-4 82-83 0.00845625 7.5E-5 0.0 7.5E-5 2.375E-4 84-85 0.00958125 7.5E-5 0.0 7.5E-5 2.375E-4 86-87 0.011 7.5E-5 0.0 7.5E-5 2.375E-4 88-89 0.01276875 7.5E-5 0.0 7.5E-5 2.4375E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGTAT 362525 0.0 62.35143 6 TGGTATC 360755 0.0 62.348045 7 AGTGGTA 368175 0.0 61.47081 5 CAGTGGT 367705 0.0 61.38637 4 GTATCAA 369795 0.0 61.082363 9 GGTATCA 370210 0.0 60.92627 8 AAGCAGT 387015 0.0 59.15559 1 AGCAGTG 396330 0.0 57.604095 2 GCAGTGG 400015 0.0 56.93842 3 AGTACTT 11680 0.0 50.58235 20-21 GTACTTT 11370 0.0 50.239773 22-23 GTCACCG 11110 0.0 49.59226 22-23 TCACCGG 11155 0.0 48.90796 22-23 GAGTACT 14285 0.0 48.18821 20-21 GAGTCAC 15890 0.0 43.06591 20-21 TACTTTT 13525 0.0 41.98522 22-23 TAACGCC 14895 0.0 41.76152 54-55 CTTAACG 14960 0.0 41.6279 52-53 AGTCACC 13380 0.0 41.53149 20-21 CTATTCG 14840 0.0 41.333553 90-91 >>END_MODULE