FastQCFastQC Report
Sat 18 Jun 2016
SRR799684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR799684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8000000
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3309594.1369875No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA935441.1693No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA521600.652No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA110850.1385625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3370500.058.4696587
GTATCAA3376100.058.3988889
GTGGTAT3381950.058.3217246
GGTATCA3401400.057.9754648
AGTGGTA3512950.056.1121335
CAGTGGT3530850.055.81714
AAGCAGT3583650.054.9182781
AGCAGTG3671750.053.6916772
GCAGTGG3723850.052.9016763
AGTACTT507250.042.85231820-21
GTACTTT499100.042.8055722-23
GAGTACT534800.041.93821720-21
TAACGCC134500.041.83437754-55
CTTAACG134600.041.67933352-53
CTATACC110250.041.54810338-39
AGAGTAC689600.041.22658518-19
TACTTTT523500.040.9839422-23
AACGCCC139100.040.93024454-55
TCTATAC106150.040.8283838-39
GGTTTCG129600.040.74107428-29
Produced by FastQC (version 0.11.5)