##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR799684_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8000000 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.1322135 31.0 31.0 33.0 30.0 34.0 2 31.3714205 31.0 31.0 34.0 30.0 34.0 3 31.58577575 31.0 31.0 34.0 30.0 34.0 4 35.121727875 37.0 35.0 37.0 33.0 37.0 5 35.116623625 35.0 35.0 37.0 33.0 37.0 6 34.85633875 36.0 35.0 37.0 32.0 37.0 7 34.87693325 35.0 35.0 37.0 32.0 37.0 8 34.850296875 36.0 35.0 37.0 32.0 37.0 9 36.164067625 38.0 35.0 39.0 31.0 39.0 10-11 36.3604808125 38.0 35.0 39.0 32.0 39.0 12-13 36.183437437500004 38.0 35.0 39.0 31.5 39.0 14-15 37.4608086875 39.0 36.0 40.0 32.0 41.0 16-17 36.1256954375 39.0 35.5 40.0 28.0 41.0 18-19 33.2409885 38.0 32.5 40.0 10.0 41.0 20-21 36.1697151875 38.0 33.5 40.0 29.0 41.0 22-23 36.7465294375 38.5 36.0 40.0 31.0 41.0 24-25 35.579691249999996 38.0 34.0 40.0 27.0 41.0 26-27 34.3163064375 38.0 32.5 40.0 23.5 41.0 28-29 34.40809125 38.0 33.0 40.0 23.5 41.0 30-31 33.9836751875 37.5 32.0 40.0 21.0 41.0 32-33 33.425330375 37.0 31.0 40.0 18.0 41.0 34-35 33.4813098125 37.0 31.0 40.0 19.0 41.0 36-37 33.4108455625 37.0 31.0 39.5 19.0 41.0 38-39 33.023021937500005 36.0 30.0 39.0 18.0 41.0 40-41 32.926149 36.0 30.0 39.0 18.0 41.0 42-43 32.976236687500005 36.0 30.5 39.0 18.5 41.0 44-45 31.5890361875 35.0 28.5 39.0 11.5 41.0 46-47 32.1534719375 35.0 29.5 39.0 16.0 41.0 48-49 32.058558125 35.0 29.5 39.0 15.5 40.5 50-51 31.2688048125 35.0 28.0 39.0 12.0 40.0 52-53 31.2263933125 35.0 27.5 39.0 12.5 40.0 54-55 31.084329812500002 35.0 27.5 38.5 12.5 40.0 56-57 30.708228625 34.5 26.5 38.0 11.5 40.0 58-59 30.758176312499998 34.0 27.0 38.0 12.0 40.0 60-61 30.43444775 34.0 26.5 37.5 11.5 40.0 62-63 30.2714903125 34.0 26.0 37.0 11.5 40.0 64-65 29.9529088125 34.0 26.0 36.5 11.0 39.0 66-67 29.6605870625 33.5 26.0 36.0 10.5 39.0 68-69 29.444167812499998 33.0 26.0 35.5 11.0 39.0 70-71 29.1024325 33.0 25.5 35.0 10.0 38.5 72-73 29.040169 33.0 26.0 35.0 10.0 37.5 74-75 28.801137375 33.0 25.5 35.0 10.0 37.0 76-77 28.51597825 32.5 25.0 35.0 10.0 36.5 78-79 28.3718840625 32.0 25.0 35.0 10.0 36.0 80-81 28.1606854375 32.0 25.0 35.0 10.0 36.0 82-83 28.091642375 32.0 25.0 35.0 10.0 35.5 84-85 27.837547375 32.0 24.5 35.0 9.0 35.0 86-87 27.63023325 32.0 24.0 35.0 8.0 35.0 88-89 27.591169375 32.0 24.0 35.0 8.0 35.0 90-91 27.465742312499998 32.0 24.0 35.0 8.0 35.0 92-93 27.4382503125 32.0 24.0 35.0 8.0 35.0 94-95 27.435105812499998 32.0 24.0 35.0 8.0 35.0 96-97 27.3012963125 32.0 24.0 34.0 8.0 35.0 98-99 27.225936812500002 32.0 23.5 34.0 8.0 35.0 100-101 25.533846812500002 29.0 20.5 33.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.06716166596645223 1101 2 -0.5193532414099415 1101 3 -0.03854351632904596 1101 4 -0.01431193380485496 1101 5 -0.2636549823478873 1101 6 0.16549768771399442 1101 7 0.14451377975640156 1101 8 0.2135375247349529 1101 9 0.547828371969473 1101 10-11 0.30551821126427825 1101 12-13 0.5001389393787008 1101 14-15 0.3229131741473452 1101 16-17 1.928896013859898 1101 18-19 6.526487604739689 1101 20-21 1.776063205100435 1101 22-23 0.7774363334012335 1101 24-25 0.32959401604929894 1101 26-27 0.3208365350398239 1101 28-29 0.6670708874278546 1101 30-31 0.48705878823388815 1101 32-33 0.5131328403529736 1101 34-35 0.44571229200715123 1101 36-37 0.23873599510429955 1101 38-39 0.24368296986023097 1101 40-41 0.32456998066903253 1101 42-43 0.2924045714746697 1101 44-45 2.239814800601806 1101 46-47 0.9571663979755236 1101 48-49 0.6031707365224648 1101 50-51 1.4085045528592488 1101 52-53 0.9523127091781376 1101 54-55 0.4853266336772002 1101 56-57 0.7751377493082856 1101 58-59 0.33405012271784784 1101 60-61 0.15970605530403503 1101 62-63 0.0433258589938319 1101 64-65 0.10303332637430529 1101 66-67 0.11571502425059421 1101 68-69 0.09844490024172714 1101 70-71 0.1207391310077206 1101 72-73 0.15579420065042626 1101 74-75 0.2226513752508552 1101 76-77 0.08372862664174363 1101 78-79 0.004160100689510671 1101 80-81 0.0659245097284007 1101 82-83 0.06743063615481404 1101 84-85 0.1412239519573717 1101 86-87 0.21096477866853647 1101 88-89 0.1268013117429021 1101 90-91 0.025506532767266776 1101 92-93 -0.011762700745812538 1101 94-95 0.024786215140309764 1101 96-97 -0.010007178086990365 1101 98-99 0.020816805387390502 1101 100-101 0.01456244906275117 1102 1 -0.39715826525262443 1102 2 -0.39159889605987175 1102 3 -0.11347849443831493 1102 4 -1.410732033037462 1102 5 -0.8468028642817913 1102 6 -0.5422172727435921 1102 7 -0.5488858408416348 1102 8 -0.352894374987045 1102 9 -0.14392927225330254 1102 10-11 -0.35683345742203443 1102 12-13 -0.14084688828199887 1102 14-15 -0.4272927991890896 1102 16-17 1.1200077385173728 1102 18-19 5.657062481766108 1102 20-21 0.8738110037351419 1102 22-23 0.014738053441689658 1102 24-25 -0.34775947569670507 1102 26-27 -0.28767030490671885 1102 28-29 0.009790130219862192 1102 30-31 -0.1594279882091243 1102 32-33 -0.23985524065929553 1102 34-35 -0.2699934408216009 1102 36-37 -0.3024012816229842 1102 38-39 -0.3452760421401777 1102 40-41 -0.3442119461424724 1102 42-43 -0.38646273391849917 1102 44-45 1.5802338525395214 1102 46-47 0.35541239363115906 1102 48-49 0.05043931053393891 1102 50-51 0.859773304194853 1102 52-53 0.4343495254437073 1102 54-55 0.0073990878017617945 1102 56-57 0.3262991024967654 1102 58-59 -0.22177008669762444 1102 60-61 -0.4115704035692751 1102 62-63 -0.5277797904525059 1102 64-65 -0.5174776852040779 1102 66-67 -0.45454599701408327 1102 68-69 -0.49602959636809985 1102 70-71 -0.45951851279169276 1102 72-73 -0.5201131656246325 1102 74-75 -0.3706974711400619 1102 76-77 -0.4453574210970963 1102 78-79 -0.5351448360762916 1102 80-81 -0.5384768290516604 1102 82-83 -0.5120298109133934 1102 84-85 -0.38524514780304386 1102 86-87 -0.37015565420031393 1102 88-89 -0.4741309042179651 1102 90-91 -0.49725058335908656 1102 92-93 -0.5425930185420782 1102 94-95 -0.5033206154863734 1102 96-97 -0.5141990474867377 1102 98-99 -0.5245373867790946 1102 100-101 -0.5772849899104635 1103 1 0.0773975926302164 1103 2 0.22370531658260617 1103 3 0.2115953805102464 1103 4 0.06357167846028489 1103 5 -0.1516868622152927 1103 6 0.07501478175802845 1103 7 0.09318084918406555 1103 8 0.1316332724214746 1103 9 0.34189321565187214 1103 10-11 0.22300191341467013 1103 12-13 0.3883720271705613 1103 14-15 0.1813501553490653 1103 16-17 1.5684648787438533 1103 18-19 6.274683557374264 1103 20-21 1.6158587607601191 1103 22-23 0.6023366188369152 1103 24-25 0.14332111127569647 1103 26-27 -0.0660797338558794 1103 28-29 0.18309648803442258 1103 30-31 0.06801434000209383 1103 32-33 0.06897579189062242 1103 34-35 0.0037368827783339498 1103 36-37 0.024501322492781696 1103 38-39 -0.011506488773576962 1103 40-41 0.11279166555376463 1103 42-43 0.03929491391053119 1103 44-45 1.939318355672654 1103 46-47 0.7357763419053533 1103 48-49 0.40593963388665344 1103 50-51 1.3108645806375137 1103 52-53 0.817722630930632 1103 54-55 0.3114321404224185 1103 56-57 0.6741436617377801 1103 58-59 0.18370023208726138 1103 60-61 0.02251010386460095 1103 62-63 -0.023322008141555273 1103 64-65 -0.050301661467518244 1103 66-67 -0.05715153228355874 1103 68-69 -0.08750859657858712 1103 70-71 0.05026563627079028 1103 72-73 0.02228219990718827 1103 74-75 0.09706112034981373 1103 76-77 -0.0026668893476511357 1103 78-79 0.0022829525261194306 1103 80-81 0.027657935963677716 1103 82-83 0.034546403345967036 1103 84-85 0.10843676646354794 1103 86-87 0.04866689221795539 1103 88-89 -0.02617620837169099 1103 90-91 -0.013755983776956526 1103 92-93 -0.02885257125250007 1103 94-95 -0.07717593212597151 1103 96-97 -0.06437916731323767 1103 98-99 -0.06209351573987831 1103 100-101 -0.03891116499550051 1104 1 0.17141524561565546 1104 2 0.3313003295348551 1104 3 0.3002086051115924 1104 4 0.15709267575905272 1104 5 0.19991649117233834 1104 6 0.2725775642634076 1104 7 -0.5473853447761314 1104 8 -0.05707102874073655 1104 9 0.2803689021978144 1104 10-11 -0.48817030330251754 1104 12-13 -0.2595094529399944 1104 14-15 -0.026012790612860215 1104 16-17 1.7909559833525606 1104 18-19 6.452299545229888 1104 20-21 1.6188704992339353 1104 22-23 0.11291281075919812 1104 24-25 0.04535024803209353 1104 26-27 -0.3738197722044063 1104 28-29 -0.9541891539342657 1104 30-31 -1.2432554399718612 1104 32-33 -1.3056391347601704 1104 34-35 -1.2443351855783433 1104 36-37 -1.4585785919181262 1104 38-39 -1.486671495054562 1104 40-41 -1.5110534699104825 1104 42-43 -1.5863473304074844 1104 44-45 0.36344094787442316 1104 46-47 -0.7848133559692556 1104 48-49 -1.0622157669024759 1104 50-51 -0.4698503730455741 1104 52-53 -0.7366952921783216 1104 54-55 -0.7862313409173254 1104 56-57 0.02918657027260707 1104 58-59 -1.284452566578235 1104 60-61 -1.6388900525546717 1104 62-63 -1.1430846833724289 1104 64-65 -1.6670445722180567 1104 66-67 -1.4381362249320269 1104 68-69 -1.5073672518284909 1104 70-71 -1.5033462073809325 1104 72-73 -1.271309922848836 1104 74-75 -1.355334632174369 1104 76-77 -1.6081681476289198 1104 78-79 -1.0838658208811935 1104 80-81 -1.3146465964547325 1104 82-83 -1.3674165765574813 1104 84-85 -1.4628948430205746 1104 86-87 -1.5708999071324463 1104 88-89 -1.6880778431545629 1104 90-91 -1.5403774042413367 1104 92-93 -1.7027531621035692 1104 94-95 -1.7337675892842803 1104 96-97 -1.8261867183886409 1104 98-99 -1.7125700166946771 1104 100-101 -1.8745065962530845 1105 1 -0.09401766421284918 1105 2 -0.029885044826144735 1105 3 -0.08399302393815589 1105 4 0.03214415012579508 1105 5 0.06452380100795807 1105 6 -3.674026672369667 1105 7 -0.6867133781375543 1105 8 -0.2363143142837245 1105 9 0.33537161041477503 1105 10-11 0.23582017215972684 1105 12-13 0.4370567429957788 1105 14-15 0.2779122255941786 1105 16-17 -26.174543530487597 1105 18-19 0.7068775751448477 1105 20-21 0.6396717624646868 1105 22-23 -2.3481589872232007 1105 24-25 0.2338705733480424 1105 26-27 0.5019732623066844 1105 28-29 0.7845540032338505 1105 30-31 0.664017193222854 1105 32-33 0.7313401366735945 1105 34-35 0.8964150327852494 1105 36-37 0.8146543638496198 1105 38-39 0.9783936396334738 1105 40-41 0.8175505154677651 1105 42-43 0.8068663732858639 1105 44-45 2.734850474163217 1105 46-47 1.4566536932516065 1105 48-49 1.164230515579213 1105 50-51 2.0535223441630848 1105 52-53 1.5613142404128908 1105 54-55 1.1437630399089684 1105 56-57 1.4972897465107877 1105 58-59 0.8917110553050804 1105 60-61 0.6505459030341747 1105 62-63 0.7022152412522011 1105 64-65 0.7988741962571844 1105 66-67 0.7419463363627194 1105 68-69 0.8080324310230189 1105 70-71 0.5743480318200689 1105 72-73 0.6120052972290253 1105 74-75 0.6582753874111802 1105 76-77 0.6135423419750161 1105 78-79 0.7340132679863771 1105 80-81 0.6289478936739954 1105 82-83 0.6868791171643345 1105 84-85 0.5758853956633025 1105 86-87 0.7182713046351132 1105 88-89 0.5209181742394726 1105 90-91 0.46885816455931106 1105 92-93 0.46413740355196254 1105 94-95 0.450062851083036 1105 96-97 0.35909743528349125 1105 98-99 0.3541447407543288 1105 100-101 0.20603450671629986 1106 1 -0.6767021772230386 1106 2 -0.6799538344031681 1106 3 -0.33840276070884556 1106 4 -0.24603658331225375 1106 5 -0.49748951241909367 1106 6 -0.19152173467403344 1106 7 -0.10764628637128482 1106 8 -0.06107340865165156 1106 9 -0.0034216407937819326 1106 10-11 -0.13401608418604383 1106 12-13 -0.5167354914226223 1106 14-15 -0.45546830060764876 1106 16-17 0.8647476340432547 1106 18-19 5.831675878293215 1106 20-21 0.6208952680193676 1106 22-23 0.1590035452485381 1106 24-25 -0.3498129410667872 1106 26-27 -0.7567857146620582 1106 28-29 -0.18599183941786634 1106 30-31 -0.20214327425019718 1106 32-33 -0.3374528669629342 1106 34-35 -0.3268495644141396 1106 36-37 -0.34047366320535133 1106 38-39 -0.366548561023059 1106 40-41 -1.0738245015322327 1106 42-43 -1.0629897541167992 1106 44-45 1.2306342682630955 1106 46-47 0.3055093041878756 1106 48-49 -0.5314777597253055 1106 50-51 0.5463105304825824 1106 52-53 0.33017375543707317 1106 54-55 -0.040415169172341336 1106 56-57 0.33345439406627264 1106 58-59 -0.30024342015020977 1106 60-61 -0.9638121994961324 1106 62-63 -0.7669388345498547 1106 64-65 -1.1313428439694384 1106 66-67 -1.180200843515923 1106 68-69 -0.9427972562470934 1106 70-71 -1.0370559060039248 1106 72-73 -0.9413803286711868 1106 74-75 -1.0428505067273974 1106 76-77 -0.42619506674061824 1106 78-79 -0.3872211447840286 1106 80-81 -0.34072935635653323 1106 82-83 -0.7664253065384194 1106 84-85 -1.066820449912747 1106 86-87 -1.6701037040356645 1106 88-89 -0.7326769699118039 1106 90-91 -0.5069075246291348 1106 92-93 -0.5102583953307516 1106 94-95 -0.4630569979085628 1106 96-97 -0.42779560571161923 1106 98-99 -0.401560192218124 1106 100-101 -0.3629203734687714 1107 1 0.07070132024776044 1107 2 0.14392168677046868 1107 3 0.09025527245781717 1107 4 0.07837277264179932 1107 5 0.08319403514600765 1107 6 0.1638725665454146 1107 7 0.11887745970599894 1107 8 0.15467999757969153 1107 9 0.26985311362589215 1107 10-11 0.14308778418244827 1107 12-13 0.21116671860697522 1107 14-15 0.05924327386098582 1107 16-17 0.5559104684198246 1107 18-19 5.989436190553196 1107 20-21 1.4714733750291558 1107 22-23 0.5811758748560152 1107 24-25 0.07733657519216308 1107 26-27 0.022293325210135606 1107 28-29 0.13675513561225472 1107 30-31 -0.19454265418885797 1107 32-33 0.014214514455602512 1107 34-35 0.1270424471267546 1107 36-37 -0.029693820701261586 1107 38-39 -0.051622544763226585 1107 40-41 0.0538181476958286 1107 42-43 0.08209224988974739 1107 44-45 1.9644841962217896 1107 46-47 0.7467120508742191 1107 48-49 0.4124011985962497 1107 50-51 1.2790840356282587 1107 52-53 0.8674702012730009 1107 54-55 0.41736059039639173 1107 56-57 0.790991751856474 1107 58-59 0.2867560442298007 1107 60-61 0.13033707384073878 1107 62-63 0.1407852599238666 1107 64-65 0.09360899977822257 1107 66-67 0.06853770200534726 1107 68-69 0.12419471369174317 1107 70-71 0.0880488203723111 1107 72-73 0.20962885190469294 1107 74-75 0.18362471412253 1107 76-77 0.1634996460945679 1107 78-79 0.06346231628912591 1107 80-81 0.04721118048809103 1107 82-83 0.0927677562576541 1107 84-85 0.12810594305483747 1107 86-87 0.13265061956400004 1107 88-89 0.004165798938622345 1107 90-91 -0.018994911508695367 1107 92-93 -0.05548394255412248 1107 94-95 0.009228500586427657 1107 96-97 -0.11604187153642087 1107 98-99 -0.10411251130107857 1107 100-101 -0.09737410303679184 1108 1 0.19252055083759956 1108 2 0.23063281349353204 1108 3 0.19090581233340131 1108 4 0.3097505874088924 1108 5 0.3322059515682909 1108 6 0.20158637661337764 1108 7 0.05044331024325288 1108 8 0.12247187874876175 1108 9 0.6167718096844936 1108 10-11 0.36142002678442964 1108 12-13 0.4458427021600073 1108 14-15 0.3250948496155672 1108 16-17 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0.3829217132436895 2109 3 0.2759766787984397 2109 4 0.2700529764298949 2109 5 0.16061687672641511 2109 6 0.23184348167738733 2109 7 0.25054509171104655 2109 8 0.1828763594965892 2109 9 0.4182027903829493 2109 10-11 0.3060359663623373 2109 12-13 0.14041792023910205 2109 14-15 0.19036645466874802 2109 16-17 1.8350686348388052 2109 18-19 6.544894044887808 2109 20-21 0.20482356697409898 2109 22-23 0.012354813340536452 2109 24-25 0.0676160777536694 2109 26-27 0.08732136831643089 2109 28-29 -0.009570557747281327 2109 30-31 0.03979953265630343 2109 32-33 0.2069666509871979 2109 34-35 0.05568485106994814 2109 36-37 -0.07054654602053034 2109 38-39 -0.15543654081959346 2109 40-41 0.21626991118062477 2109 42-43 -0.2139994339554363 2109 44-45 1.8511779311387144 2109 46-47 0.77522183138937 2109 48-49 0.4698783867708869 2109 50-51 1.4636199387603739 2109 52-53 1.0388170970024646 2109 54-55 0.7340322326888362 2109 56-57 1.134504471745032 2109 58-59 0.7543721124659868 2109 60-61 0.6418792178493398 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-2.289087842832622 2110 86-87 0.16146954640628408 2110 88-89 0.7702597806496954 2110 90-91 0.8511795301499383 2110 92-93 0.9667853295463118 2110 94-95 0.9347625361442198 2110 96-97 0.8151104620050518 2110 98-99 0.7915913640089194 2110 100-101 0.8901030240425278 2111 1 0.20300232436830612 2111 2 0.222173976815089 2111 3 0.16854043310564748 2111 4 0.30073710930156494 2111 5 0.28049537969327076 2111 6 -0.8480187033990134 2111 7 -1.0166196574657178 2111 8 -1.7109923465091512 2111 9 0.17963519970025743 2111 10-11 -0.10653475699172787 2111 12-13 0.26834619302235296 2111 14-15 0.4325298376990361 2111 16-17 1.7090543717328401 2111 18-19 -21.310502822362082 2111 20-21 -5.249381995501956 2111 22-23 -0.590155217323769 2111 24-25 0.5633011305151996 2111 26-27 0.862997845220967 2111 28-29 1.0803529337559326 2111 30-31 0.11739704590112154 2111 32-33 -0.23644283834671143 2111 34-35 -0.018054966933938488 2111 36-37 0.5398843965189002 2111 38-39 0.7474965336061672 2111 40-41 0.7320142706239778 2111 42-43 0.8774653964242773 2111 44-45 2.0784732388272857 2111 46-47 1.3642023329095245 2111 48-49 1.112312424262818 2111 50-51 0.868650562449993 2111 52-53 1.2226650998501967 2111 54-55 0.9228349812860728 2111 56-57 1.571308897866352 2111 58-59 1.1718279769883466 2111 60-61 1.0277074585071269 2111 62-63 0.8833080405293146 2111 64-65 0.8433236010292298 2111 66-67 0.927868548182385 2111 68-69 0.9721291004910384 2111 70-71 0.898715383791572 2111 72-73 0.9248775062972996 2111 74-75 0.9712352095902475 2111 76-77 0.8656967351234677 2111 78-79 0.5925356734404801 2111 80-81 0.5436914052513728 2111 82-83 0.8502285205966338 2111 84-85 0.9742766297198919 2111 86-87 1.0071775811626829 2111 88-89 0.9574273371327209 2111 90-91 0.9227187728947968 2111 92-93 0.9590479522689037 2111 94-95 0.8602458826298864 2111 96-97 0.8040935458669018 2111 98-99 0.855955493710109 2111 100-101 0.8363872987890808 2112 1 0.10368656102289009 2112 2 0.12252133716861735 2112 3 0.03854787883470934 2112 4 0.22047275232990415 2112 5 0.12945638792024283 2112 6 0.27287398785626493 2112 7 0.2754651675202737 2112 8 0.22934106753947248 2112 9 0.45031971731950193 2112 10-11 -0.16855021911589319 2112 12-13 0.0022925538565061743 2112 14-15 0.30544528424603357 2112 16-17 -0.23630311410400395 2112 18-19 -21.303744050249808 2112 20-21 -4.274898253179714 2112 22-23 -0.8872632040239807 2112 24-25 0.24079972958037388 2112 26-27 0.46887351296295066 2112 28-29 0.5820845398283865 2112 30-31 0.4349309568302928 2112 32-33 0.6615423173866404 2112 34-35 0.3424870638740245 2112 36-37 0.25298836671506564 2112 38-39 0.3928051203396734 2112 40-41 0.4519008237776134 2112 42-43 0.16772027770546316 2112 44-45 -23.999773674375447 2112 46-47 -9.071271004685602 2112 48-49 -2.4130633841766027 2112 50-51 -28.953230374852097 2112 52-53 -17.696141581006337 2112 54-55 -6.178135346231031 2112 56-57 -23.885922174522157 2112 58-59 -8.721203058553748 2112 60-61 -1.6300020033800173 2112 62-63 -0.11037751030521648 2112 64-65 0.22475729057214267 2112 66-67 0.24291751860242883 2112 68-69 0.056662035456849225 2112 70-71 0.13001596922066483 2112 72-73 -0.39106312153396416 2112 74-75 -0.541712198093748 2112 76-77 0.08032493034777488 2112 78-79 0.07009198277999218 2112 80-81 0.09206992971563466 2112 82-83 0.20518158841140988 2112 84-85 0.5637385916547721 2112 86-87 0.5898038200919196 2112 88-89 0.5854151390303279 2112 90-91 0.619198280692359 2112 92-93 0.5712114867421576 2112 94-95 0.7599549253534938 2112 96-97 0.7391280623262197 2112 98-99 0.6606711116356294 2112 100-101 0.6114452349272348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 144.0 8 490.0 9 996.0 10 1914.0 11 3488.0 12 6327.0 13 12504.0 14 22305.0 15 34465.0 16 53079.0 17 78849.0 18 102199.0 19 116235.0 20 127856.0 21 143341.0 22 161761.0 23 186357.0 24 214228.0 25 238194.0 26 255733.0 27 269758.0 28 288927.0 29 320738.0 30 363179.0 31 419158.0 32 489147.0 33 572515.0 34 669893.0 35 809846.0 36 871512.0 37 762117.0 38 359617.0 39 43064.0 40 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 15.824598047615176 44.35650721982152 20.74923549313038 19.06965923943292 2 16.8512375 46.3009 17.5732375 19.274625 3 44.144149999999996 19.860475 17.6449875 18.3503875 4 18.016413387462123 19.387362369159842 17.60380993190818 44.99241431146986 5 18.023949968374954 45.97717642372759 19.0892311710875 16.909642436809953 6 44.36605334100688 19.895524548568496 17.88194266488028 17.856479445544338 7 17.635275 19.7481875 45.4960875 17.12045 8 44.0320875 20.404600000000002 18.311887499999997 17.251425 9 43.4708 20.037125 18.9352375 17.5568375 10-11 16.66030625 33.3538875 32.451468750000004 17.5343375 12-13 16.635131249999997 20.040425 32.186749999999996 31.13769375 14-15 16.6611875 46.73874375 19.138918750000002 17.46115 16-17 30.132599484092122 19.677139400018902 19.18193793542459 31.00832318046438 18-19 16.56476905011945 34.24098918334133 19.082569694183633 30.11167207235559 20-21 30.084671292335646 33.133604066802036 19.28708464354232 17.49463999732 22-23 29.964156859318624 19.761758645769408 32.62068302154882 17.653401473363147 24-25 22.874347013747666 28.769907713484677 22.412545686068846 25.94319958669881 26-27 24.51283837187747 23.272138883642338 28.133311366565074 24.08171137791512 28-29 37.283528469847646 19.68226071271651 25.55866251747666 17.475548299959186 30-31 29.85951503991521 24.124390124846006 27.311019250188956 18.70507558504983 32-33 23.50161295383116 26.21432792007019 29.818745664799856 20.465313461298795 34-35 21.329627700668766 25.328487185485653 28.93701647403181 24.404868639813767 36-37 25.02034065805357 22.872043417070554 31.28350083568886 20.82411508918702 38-39 26.140918334681047 22.7089976838695 29.23398818072193 21.91609580072753 40-41 20.426735860544902 26.548215317195623 31.732012389597568 21.293036432661907 42-43 20.560844627616625 23.83263099992556 31.87261984725897 23.733904525198845 44-45 20.584791742135895 28.523834033264333 28.30905549928647 22.5823187253133 46-47 23.645443906067136 22.715702926144044 28.75879429177689 24.880058876011933 48-49 21.542304999715828 25.509879173528887 28.517647834828132 24.430167991927153 50-51 23.57469995561662 27.10748070236089 28.12753321905031 21.19028612297219 52-53 24.01648100412025 23.043318260829565 30.745651436412857 22.194549298637327 54-55 22.714422802114235 25.56804167398058 28.773338883620635 22.94419664028455 56-57 23.65720832563594 25.248178227484118 26.84940783608164 24.24520561079831 58-59 28.020071685663094 23.62362476852911 26.170117073582933 22.18618647222487 60-61 24.478825353187865 25.81370070859139 26.035097627334792 23.67237631088595 62-63 24.489436987238534 27.04429269058542 26.204674208303736 22.26159611387231 64-65 21.83929375 25.668593750000003 28.30689375 24.18521875 66-67 24.1630375 25.324393750000002 28.611793749999997 21.900775 68-69 26.807501939535005 24.559510084050785 26.8604957922106 21.772492184203607 70-71 21.171341865285655 26.044736030420275 29.938696319435394 22.845225784858673 72-73 21.956326548010285 24.115749035339288 28.99562160474992 24.932302811900506 74-75 22.250922525369173 28.102490582556776 27.545965954265395 22.100620937808653 76-77 23.79237614942895 23.68148020923413 28.001290525487875 24.524853115849037 78-79 21.84424935688126 25.72924343210748 26.577430783835787 25.849076427175472 80-81 24.07318066319976 25.96741032965611 27.324597629166014 22.63481137797811 82-83 24.028620698987215 24.471771400913852 28.777082690484708 22.722525209614226 84-85 25.06301143959599 25.181230744594735 26.47874293264006 23.277014883169212 86-87 23.55570158396308 25.01725788692464 26.88912220933953 24.537918319772754 88-89 26.424624416929287 24.10754782393913 25.718586431528173 23.749241327603414 90-91 24.02538579442514 26.10377693160963 25.64189487331603 24.2289424006492 92-93 23.47869418732835 27.32212926750945 26.954339606745194 22.244836938417013 94-95 21.864362298703792 26.15482721209031 27.623696788329443 24.357113700876457 96-97 24.660988193782245 24.391112502225788 27.455182853906063 23.492716450085904 98-99 26.716825 24.36559375 26.417524999999998 22.50005625 100-101 22.047764121520416 27.015640950830345 28.029947276555657 22.906647651093582 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 76179.0 1 45845.5 2 12419.5 3 7681.5 4 5366.0 5 4101.5 6 3221.5 7 2855.5 8 2590.5 9 2448.0 10 3069.5 11 11316.5 12 52124.5 13 56066.0 14 23946.0 15 20480.0 16 20096.0 17 20690.5 18 21536.0 19 22498.5 20 23762.0 21 24995.5 22 26485.5 23 28728.5 24 31207.5 25 33913.5 26 37279.5 27 41195.0 28 45497.0 29 50926.5 30 57321.5 31 64280.0 32 71993.0 33 80879.5 34 91591.5 35 103526.0 36 115556.0 37 124849.0 38 130529.5 39 134193.0 40 138824.5 41 145438.5 42 153978.0 43 164882.0 44 177613.5 45 191249.0 46 208799.5 47 229500.0 48 247546.0 49 259971.5 50 279052.5 51 311153.5 52 360236.5 53 436721.0 54 463514.5 55 432559.0 56 400666.0 57 349876.5 58 288292.0 59 234152.0 60 185805.0 61 144801.0 62 112484.0 63 85644.0 64 66402.5 65 52614.5 66 41547.0 67 33228.0 68 25979.5 69 19693.0 70 15201.5 71 12233.0 72 10121.5 73 7785.5 74 5681.5 75 4186.0 76 2865.0 77 1835.5 78 1139.5 79 658.5 80 345.5 81 205.5 82 122.5 83 72.5 84 50.0 85 40.0 86 28.5 87 16.5 88 13.5 89 8.5 90 6.0 91 4.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.058124999999999996 2 0.0 3 0.0 4 8.375E-4 5 0.07919999999999999 6 0.002825 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.2081375 18-19 6.895925 20-21 4.9999999999999996E-5 22-23 4.375E-5 24-25 2.875E-4 26-27 4.6249999999999997E-4 28-29 5.625000000000001E-4 30-31 0.0013062500000000001 32-33 0.0010125 34-35 0.00133125 36-37 1.625E-4 38-39 2.3750000000000003E-4 40-41 7.875E-4 42-43 0.00149375 44-45 2.5886125 46-47 0.00171875 48-49 0.69798125 50-51 2.8070874999999997 52-53 2.4999999999999998E-5 54-55 0.69583125 56-57 0.3992 58-59 9.249999999999999E-4 60-61 0.00143125 62-63 7.125E-4 64-65 0.0 66-67 0.0 68-69 1.9375000000000002E-4 70-71 9.0625E-4 72-73 4.6249999999999997E-4 74-75 0.00105625 76-77 0.0019749999999999998 78-79 0.00145625 80-81 9.0625E-4 82-83 0.0015687499999999998 84-85 0.00575625 86-87 4.3125E-4 88-89 6.875E-5 90-91 1.75E-4 92-93 5.874999999999999E-4 94-95 5.625E-5 96-97 2.5625E-4 98-99 0.0 100-101 0.0468375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8000000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.9353063154073 #Duplication Level Percentage of deduplicated Percentage of total 1 82.50846217325302 44.50119180927598 2 10.060419961282625 10.85223664546833 3 2.832643871036066 4.58338544600374 4 1.2823274593778344 2.7665089727280603 5 0.7320743160640429 1.9742326241278227 6 0.5040172815600362 1.6310595881519663 7 0.342779766681957 1.2941552200300637 8 0.27268528535294206 1.1765891514572144 9 0.21397997908191402 1.0386968145432731 >10 1.1273541279103805 11.05768717451662 >50 0.07781781973911915 2.8395243917991544 >100 0.03536862907832879 4.12457756110979 >500 0.006877962485285441 2.465367852154401 >1k 0.003067670697935511 3.1167728499470835 >5k 2.4739279716432232E-5 0.06989171457469315 >10k+ 9.895711886572893E-5 6.508122184111767 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 330959 4.1369875 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 93544 1.1693 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA 52160 0.652 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA 11085 0.1385625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 1.25E-5 0.0 0.0 0.0 1.25E-5 5 1.25E-5 0.0 0.0 0.0 1.25E-5 6 1.25E-5 0.0 0.0 0.0 1.25E-5 7 1.25E-5 0.0 0.0 0.0 2.5E-5 8 1.25E-5 0.0 0.0 0.0 2.5E-5 9 2.5E-5 0.0 0.0 0.0 2.5E-5 10-11 2.5E-5 0.0 0.0 0.0 2.5E-5 12-13 6.874999999999999E-5 0.0 0.0 0.0 3.125E-5 14-15 8.75E-5 0.0 0.0 0.0 3.75E-5 16-17 9.375E-5 0.0 0.0 0.0 5.625E-5 18-19 1.25E-4 0.0 0.0 6.25E-6 7.5E-5 20-21 1.6875E-4 0.0 0.0 1.25E-5 7.5E-5 22-23 1.75E-4 1.25E-5 0.0 3.75E-5 7.5E-5 24-25 2.25E-4 1.25E-5 0.0 3.75E-5 7.5E-5 26-27 3.0000000000000003E-4 1.25E-5 0.0 3.75E-5 7.5E-5 28-29 3.6875E-4 1.25E-5 0.0 3.75E-5 1.1875E-4 30-31 5.0625E-4 1.25E-5 0.0 3.75E-5 1.25E-4 32-33 6.125E-4 1.25E-5 0.0 3.75E-5 1.375E-4 34-35 7.125E-4 1.25E-5 0.0 3.75E-5 1.375E-4 36-37 8.375E-4 1.25E-5 0.0 3.75E-5 1.625E-4 38-39 9.6875E-4 1.25E-5 0.0 3.75E-5 1.8125E-4 40-41 0.00111875 1.25E-5 0.0 3.75E-5 1.9375000000000002E-4 42-43 0.0012374999999999999 1.25E-5 0.0 3.75E-5 2.0E-4 44-45 0.0013062500000000001 1.25E-5 0.0 4.375E-5 2.125E-4 46-47 0.0014312499999999998 1.25E-5 0.0 5.625E-5 2.375E-4 48-49 0.00161875 1.25E-5 0.0 6.25E-5 2.5E-4 50-51 0.0018062500000000001 1.25E-5 0.0 6.25E-5 2.5624999999999997E-4 52-53 0.001925 1.25E-5 0.0 6.25E-5 2.625E-4 54-55 0.0020875 1.8750000000000002E-5 0.0 6.25E-5 2.625E-4 56-57 0.0022375 3.75E-5 0.0 6.25E-5 2.625E-4 58-59 0.0024749999999999998 3.75E-5 0.0 6.25E-5 2.8125000000000003E-4 60-61 0.0026875 3.75E-5 0.0 6.25E-5 2.9374999999999996E-4 62-63 0.003 3.75E-5 0.0 6.25E-5 3.0625E-4 64-65 0.003225 3.75E-5 0.0 6.874999999999999E-5 3.5E-4 66-67 0.0035 3.75E-5 0.0 7.5E-5 3.5E-4 68-69 0.0039125 3.75E-5 0.0 7.5E-5 3.625E-4 70-71 0.004243749999999999 3.75E-5 0.0 7.5E-5 3.6875E-4 72-73 0.0045125 3.75E-5 0.0 7.5E-5 3.75E-4 74-75 0.0048625000000000005 3.75E-5 0.0 7.5E-5 4.0E-4 76-77 0.0053375 3.75E-5 0.0 7.5E-5 4.125E-4 78-79 0.0059125 3.75E-5 0.0 7.5E-5 4.125E-4 80-81 0.00659375 3.75E-5 0.0 7.5E-5 4.25E-4 82-83 0.00743125 3.75E-5 0.0 7.5E-5 4.25E-4 84-85 0.00866875 3.75E-5 0.0 7.5E-5 4.5625E-4 86-87 0.01010625 3.75E-5 0.0 7.5E-5 4.625E-4 88-89 0.0119375 3.75E-5 0.0 7.5E-5 4.75E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTATC 337050 0.0 58.469658 7 GTATCAA 337610 0.0 58.398888 9 GTGGTAT 338195 0.0 58.321724 6 GGTATCA 340140 0.0 57.975464 8 AGTGGTA 351295 0.0 56.112133 5 CAGTGGT 353085 0.0 55.8171 4 AAGCAGT 358365 0.0 54.918278 1 AGCAGTG 367175 0.0 53.691677 2 GCAGTGG 372385 0.0 52.901676 3 AGTACTT 50725 0.0 42.852318 20-21 GTACTTT 49910 0.0 42.80557 22-23 GAGTACT 53480 0.0 41.938217 20-21 TAACGCC 13450 0.0 41.834377 54-55 CTTAACG 13460 0.0 41.679333 52-53 CTATACC 11025 0.0 41.548103 38-39 AGAGTAC 68960 0.0 41.226585 18-19 TACTTTT 52350 0.0 40.98394 22-23 AACGCCC 13910 0.0 40.930244 54-55 TCTATAC 10615 0.0 40.82838 38-39 GGTTTCG 12960 0.0 40.741074 28-29 >>END_MODULE