##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR799683_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5848219 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.422402102246856 31.0 27.0 31.0 16.0 34.0 2 28.735134576868617 31.0 28.0 31.0 20.0 34.0 3 28.29057615660426 31.0 27.0 31.0 16.0 34.0 4 31.25950618470341 35.0 30.0 37.0 17.0 37.0 5 31.67177528748496 35.0 31.0 37.0 17.0 37.0 6 31.609423826296517 35.0 31.0 37.0 17.0 37.0 7 31.22858805390154 35.0 30.0 37.0 17.0 37.0 8 31.190000408671427 35.0 30.0 37.0 17.0 37.0 9 32.3663758145856 37.0 30.0 39.0 16.0 39.0 10-11 32.47398763623592 37.0 30.5 39.0 16.5 39.0 12-13 32.298445389955475 37.0 30.0 39.0 16.0 39.0 14-15 33.75735570094075 38.0 31.0 40.0 17.0 41.0 16-17 33.26192410031157 38.0 30.5 40.0 16.5 41.0 18-19 33.23896882110605 37.5 30.5 40.0 17.0 41.0 20-21 33.403802764568155 38.0 30.5 40.0 17.5 41.0 22-23 29.383724515104515 35.0 20.0 39.5 9.5 40.5 24-25 29.584749134736576 33.0 21.5 39.0 13.5 40.0 26-27 30.795065557565472 34.5 26.0 38.5 11.5 40.0 28-29 31.39646839490792 35.5 27.0 39.0 12.0 40.5 30-31 30.68858014722089 34.5 26.0 39.5 10.5 40.5 32-33 30.183630777164808 34.0 25.0 39.0 9.5 40.0 34-35 30.495352089242896 34.0 25.0 39.0 10.5 40.0 36-37 30.647306214079876 34.0 25.5 39.0 10.5 40.0 38-39 30.39728265990039 34.0 25.0 39.0 9.5 40.0 40-41 30.219540000126536 34.0 25.0 38.5 9.5 40.0 42-43 30.324207763081375 34.0 25.0 38.5 10.5 40.0 44-45 30.092510386495444 33.5 25.0 38.0 9.5 40.0 46-47 29.811360945956366 33.0 24.0 38.0 9.0 40.0 48-49 29.700150250871246 33.0 24.0 38.0 9.0 40.0 50-51 29.413032497586016 33.0 24.0 38.0 9.0 40.0 52-53 29.309077943216558 33.0 23.5 38.0 9.0 40.0 54-55 29.222306312400406 33.0 24.0 38.0 9.0 40.0 56-57 29.125922438951072 33.0 24.0 37.5 9.0 40.0 58-59 28.976675206588535 32.5 24.0 37.0 8.5 40.0 60-61 28.55745809108722 32.0 23.0 36.5 8.0 39.5 62-63 28.3468471341446 32.0 23.0 36.0 8.0 39.0 64-65 28.090142657106377 31.0 23.0 35.5 8.0 39.0 66-67 27.87248818828433 31.0 22.5 35.0 8.0 39.0 68-69 27.676935148974415 31.0 22.0 35.0 8.0 38.0 70-71 27.273470265049923 31.0 21.5 35.0 8.0 37.5 72-73 27.125848142827756 30.5 21.5 35.0 8.0 37.0 74-75 27.003475673534112 30.0 21.5 35.0 8.0 36.5 76-77 26.72607908493167 30.0 21.0 34.0 7.5 36.0 78-79 26.47938688684538 30.0 20.0 34.0 7.0 36.0 80-81 26.288292298903308 30.0 20.0 34.0 7.0 35.0 82-83 26.229139247350346 30.0 20.0 34.0 7.0 35.0 84-85 26.03671955513294 29.5 20.0 34.0 7.0 35.0 86-87 25.922146810849593 29.0 20.0 34.0 7.0 35.0 88-89 25.73924428958628 29.0 20.0 34.0 7.0 35.0 90-91 25.702493442875515 29.0 20.0 34.0 7.0 35.0 92-93 25.551294847200488 29.0 20.0 34.0 7.0 35.0 94-95 25.468972092187382 29.0 19.0 34.0 7.0 35.0 96-97 25.33803752903234 29.0 19.0 34.0 7.0 35.0 98-99 25.14949722300071 29.0 19.0 34.0 7.0 35.0 100-101 23.9356446808849 27.0 16.5 33.0 7.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2104 1 -0.10938442848837582 2104 2 0.1762320806658373 2104 3 0.21905325506887507 2104 4 0.10186612889970093 2104 5 -0.05473263694006647 2104 6 0.12240761847560222 2104 7 -0.13621039824129255 2104 8 0.19030072742725324 2104 9 0.44290579886006043 2104 10-11 0.26464502345857 2104 12-13 0.33103503560897707 2104 14-15 0.19081438328944245 2104 16-17 0.07823398233203704 2104 18-19 0.508894731510658 2104 20-21 0.32884432419531606 2104 22-23 -11.281638167055487 2104 24-25 -9.380622739712358 2104 26-27 -2.02868139353183 2104 28-29 -0.30344170265870574 2104 30-31 0.22209422556334246 2104 32-33 0.16931212440931986 2104 34-35 0.4411109389293415 2104 36-37 0.18217291366835298 2104 38-39 0.30347992565213033 2104 40-41 0.24225648911621533 2104 42-43 0.2326503672448439 2104 44-45 0.2893263012278169 2104 46-47 0.2808738556696255 2104 48-49 -0.023414894028768174 2104 50-51 0.3891328800046985 2104 52-53 -0.03046578684836021 2104 54-55 0.048564557132948494 2104 56-57 -0.04986151420536089 2104 58-59 -0.10317731493882576 2104 60-61 0.022445799633306507 2104 62-63 0.037790739062707246 2104 64-65 -0.22578592606241443 2104 66-67 0.032619868986216716 2104 68-69 0.10811859255265333 2104 70-71 -0.02820704316511069 2104 72-73 -0.033778331962018626 2104 74-75 0.005400079937096791 2104 76-77 0.0023128824311449137 2104 78-79 0.053006184411604096 2104 80-81 -0.25454745067990814 2104 82-83 -0.17496446872787175 2104 84-85 -0.30194374350504205 2104 86-87 -0.09808083390545974 2104 88-89 0.04934351994814534 2104 90-91 -0.21092756140335212 2104 92-93 -0.11765842490307321 2104 94-95 -0.03435066710415313 2104 96-97 -0.23186568769042637 2104 98-99 -0.07753602368702062 2104 100-101 -0.06532531160933175 2105 1 -0.11971235251431267 2105 2 -0.18824394534926014 2105 3 -0.0911520593823738 2105 4 -0.26286048761578584 2105 5 -0.27918816302428695 2105 6 -0.22604262923334417 2105 7 -0.15391661720367722 2105 8 -0.1451653456572437 2105 9 -0.0035261615315818062 2105 10-11 0.049211321251839024 2105 12-13 0.14129219186775543 2105 14-15 -0.01994683116866014 2105 16-17 -0.04892372070763429 2105 18-19 0.002733369684271736 2105 20-21 -0.04319050723131568 2105 22-23 3.966183301773089 2105 24-25 3.101687908412096 2105 26-27 0.9773665344151112 2105 28-29 0.3116248237434469 2105 30-31 0.41326811647947537 2105 32-33 0.36994218301189363 2105 34-35 0.40272789143545396 2105 36-37 0.19014993577742345 2105 38-39 0.2255962501367499 2105 40-41 0.4116189515385251 2105 42-43 0.24993826068977754 2105 44-45 0.2905980360470899 2105 46-47 0.4077228366102936 2105 48-49 0.25316682955332226 2105 50-51 0.1941584970832615 2105 52-53 0.379691272499759 2105 54-55 0.12260651965920033 2105 56-57 0.07616404820208089 2105 58-59 0.11240860456508273 2105 60-61 0.3235807347609665 2105 62-63 0.12335448890296163 2105 64-65 -0.10418963889345179 2105 66-67 -0.03300245730846996 2105 68-69 0.10719606084078848 2105 70-71 0.24169019949494341 2105 72-73 0.3259352775266606 2105 74-75 0.22043904878395537 2105 76-77 0.1675825190283433 2105 78-79 0.2095143596600728 2105 80-81 0.29300722218653164 2105 82-83 0.17739696260397153 2105 84-85 0.2112864511833621 2105 86-87 0.1882092583982029 2105 88-89 0.19342726845122016 2105 90-91 0.019696573913602577 2105 92-93 0.03386440534099222 2105 94-95 0.012456717641629922 2105 96-97 0.0659361152031046 2105 98-99 -0.02622118747579094 2105 100-101 0.036072497610728504 2106 1 -0.42870439597112053 2106 2 -0.2829686223455852 2106 3 -1.5616773935859634 2106 4 -0.7105594231791592 2106 5 -0.6298056333590871 2106 6 -0.3921878126801168 2106 7 -0.514458355312609 2106 8 -0.4280721149263904 2106 9 -0.42120665665255785 2106 10-11 -0.7085284236211677 2106 12-13 -2.5937960798381283 2106 14-15 -1.6008564120192617 2106 16-17 -1.0405887186609561 2106 18-19 -2.9115365470003454 2106 20-21 -1.8861252013571352 2106 22-23 1.1391172520997976 2106 24-25 1.2327206844051375 2106 26-27 -0.25456101057588754 2106 28-29 -0.7897373822809506 2106 30-31 -1.191792620220376 2106 32-33 -0.8566250750813005 2106 34-35 -0.5299946724325366 2106 36-37 -0.6757949734496869 2106 38-39 -1.5865051805955765 2106 40-41 -2.2367481551047845 2106 42-43 -1.101994623837065 2106 44-45 -1.1367861464443223 2106 46-47 -0.9431942281497605 2106 48-49 -0.9096152352517421 2106 50-51 -2.9406386237091446 2106 52-53 -2.991760911103473 2106 54-55 -2.213148428504063 2106 56-57 -1.4824517054891189 2106 58-59 -0.8623307002202765 2106 60-61 -2.4260409364648403 2106 62-63 -1.7623110023928739 2106 64-65 -1.2574011404992405 2106 66-67 -1.2226240246001225 2106 68-69 -1.1891407506487788 2106 70-71 -2.547987710774528 2106 72-73 -2.2431396633687584 2106 74-75 -1.5837650025251229 2106 76-77 -2.6412562117959375 2106 78-79 -2.054296242211553 2106 80-81 -2.927786509936208 2106 82-83 -1.2752835310861173 2106 84-85 -0.7425099980825429 2106 86-87 -0.650228410429758 2106 88-89 -1.5690905141751443 2106 90-91 -0.9536826113352497 2106 92-93 -1.037009519981158 2106 94-95 -0.8139789401710296 2106 96-97 -0.8285036235144965 2106 98-99 -0.8588905985851376 2106 100-101 -0.8089296113810498 2107 1 0.053995160223983873 2107 2 0.031127681315254563 2107 3 0.05073684342607976 2107 4 0.023816596227842268 2107 5 -0.13080019025682077 2107 6 -0.07536752104116218 2107 7 0.0856110499801197 2107 8 0.07867010743547809 2107 9 0.10616198998433646 2107 10-11 0.1605201816130304 2107 12-13 0.26572697704378356 2107 14-15 -0.09895007901952368 2107 16-17 0.003982718048561651 2107 18-19 0.10996641887646064 2107 20-21 -0.016712397256206657 2107 22-23 3.922954639948717 2107 24-25 3.2158682636906306 2107 26-27 1.0058011069301749 2107 28-29 0.3528089335382809 2107 30-31 0.4038569683180526 2107 32-33 0.42428135644551546 2107 34-35 0.37799914728173434 2107 36-37 0.23791926812007347 2107 38-39 0.40196105173162877 2107 40-41 0.48904148612354703 2107 42-43 0.377445845757574 2107 44-45 0.42493828036882775 2107 46-47 0.4777454148700606 2107 48-49 0.4113231491127074 2107 50-51 0.45642015970322447 2107 52-53 0.346185682016376 2107 54-55 0.16388397037774638 2107 56-57 -0.012109273183007474 2107 58-59 -0.033901291674670375 2107 60-61 0.10809567805483056 2107 62-63 0.15980054756199635 2107 64-65 0.07895233116961009 2107 66-67 0.06214315238391421 2107 68-69 0.023038877973441174 2107 70-71 0.025202703418667483 2107 72-73 0.010077197110227587 2107 74-75 0.08637529783990061 2107 76-77 0.14582348930429134 2107 78-79 0.19253168620766914 2107 80-81 0.1511216275919871 2107 82-83 0.005025788182095425 2107 84-85 -0.008475974442809786 2107 86-87 0.04035714045122063 2107 88-89 0.05606005263747349 2107 90-91 -0.026945172517987004 2107 92-93 0.006465035204698211 2107 94-95 0.033426853677408275 2107 96-97 0.015059715939212737 2107 98-99 0.08750578767136474 2107 100-101 0.05024621623931935 2108 1 0.1071403677123719 2108 2 0.10044040235145246 2108 3 0.13873897647501465 2108 4 0.1512203524674689 2108 5 0.1201929566807074 2108 6 0.14624411468034282 2108 7 0.019849683930512896 2108 8 0.08427452263198276 2108 9 0.1714090405905182 2108 10-11 -0.09515575802114995 2108 12-13 0.29024544419490894 2108 14-15 0.1562547411612556 2108 16-17 0.04466138423467214 2108 18-19 0.2667760028061821 2108 20-21 0.05080917327880741 2108 22-23 3.8195206186768864 2108 24-25 3.0480435616414425 2108 26-27 0.5995229504897743 2108 28-29 0.06684392044322252 2108 30-31 0.38503754022831416 2108 32-33 0.2070707395500193 2108 34-35 0.34896476963412226 2108 36-37 0.1811798246194627 2108 38-39 0.3000840156397402 2108 40-41 0.43773623944306905 2108 42-43 0.24548735278010625 2108 44-45 0.20535257510900706 2108 46-47 0.20121867361052992 2108 48-49 0.036396220851173666 2108 50-51 0.16848599230015182 2108 52-53 0.06258570288842336 2108 54-55 0.07582449240449662 2108 56-57 0.052109641917198246 2108 58-59 0.004960433625157634 2108 60-61 0.06347826597203365 2108 62-63 -0.00639634207230344 2108 64-65 0.04929114686485292 2108 66-67 0.05700248096986016 2108 68-69 -0.019459955190519906 2108 70-71 0.16223280498740422 2108 72-73 0.0334594562168391 2108 74-75 -0.06919735977238162 2108 76-77 0.015242396762175758 2108 78-79 0.05809042373939022 2108 80-81 0.09893698198735024 2108 82-83 0.03673452846471292 2108 84-85 0.006733983143533351 2108 86-87 -5.737235319429601E-4 2108 88-89 0.007912271981755481 2108 90-91 -0.0182000425096156 2108 92-93 -0.023594994928643587 2108 94-95 0.02911928448009604 2108 96-97 0.02890160582144574 2108 98-99 0.07026921771014472 2108 100-101 0.010164789810485075 2109 1 0.21275358869172223 2109 2 0.19937332195892665 2109 3 0.20806060792729753 2109 4 0.20254439897170684 2109 5 0.17586388940811304 2109 6 0.14561701358071844 2109 7 0.1556847921170963 2109 8 0.16802835344781997 2109 9 0.07083604803956689 2109 10-11 0.1977385720895839 2109 12-13 0.22892711948743738 2109 14-15 0.29636139912371817 2109 16-17 0.22264496957690483 2109 18-19 0.43814842767738327 2109 20-21 0.19062970646567123 2109 22-23 4.13444035513977 2109 24-25 3.191789497560954 2109 26-27 0.5173571942811712 2109 28-29 0.210397689611046 2109 30-31 0.42804095621039195 2109 32-33 0.21818670280400454 2109 34-35 0.18841548692700627 2109 36-37 0.10738909047375955 2109 38-39 0.18502943404013195 2109 40-41 0.3309272663539602 2109 42-43 0.16053809011301823 2109 44-45 0.21624957470834616 2109 46-47 0.3210825830758033 2109 48-49 0.2212170866410581 2109 50-51 0.38216028650746026 2109 52-53 0.3537242128073288 2109 54-55 0.08018627938269418 2109 56-57 -0.013538950508220182 2109 58-59 0.05974581081258634 2109 60-61 0.19953151678690162 2109 62-63 0.15639721328301803 2109 64-65 0.15450998986937847 2109 66-67 0.1925969993180665 2109 68-69 0.15685917680130856 2109 70-71 0.24490864565900594 2109 72-73 0.20728709928237166 2109 74-75 0.19517486972948106 2109 76-77 0.259912386788276 2109 78-79 0.22605288156949754 2109 80-81 0.19430505171547452 2109 82-83 0.12555586791110684 2109 84-85 0.05034251884569585 2109 86-87 0.15969999710751637 2109 88-89 0.23286237337676496 2109 90-91 0.16181211846297927 2109 92-93 -0.011039289163438326 2109 94-95 0.03986712513418311 2109 96-97 0.09491558409222023 2109 98-99 0.028384691795452 2109 100-101 0.046886375881555864 2110 1 -0.2260085638948368 2110 2 -0.19078548795761563 2110 3 -0.21499850258854636 2110 4 -0.3013517394139349 2110 5 -0.30377339092727595 2110 6 -0.24357090220330235 2110 7 -0.1959347869619812 2110 8 -0.20258412493100408 2110 9 -0.039234971006941066 2110 10-11 -0.5516259009956777 2110 12-13 -0.20888126548490504 2110 14-15 -0.2302724957133151 2110 16-17 -0.29895153085635684 2110 18-19 -0.22045077066426444 2110 20-21 -0.20620446204637943 2110 22-23 -11.530857868188704 2110 24-25 -9.9393277722986 2110 26-27 -2.469411811562381 2110 28-29 -0.7868979464328589 2110 30-31 -0.7983743436719948 2110 32-33 -0.5159235706560281 2110 34-35 -0.0654320570706517 2110 36-37 -0.03246679264485408 2110 38-39 0.003236217719138068 2110 40-41 -0.5189644553892556 2110 42-43 -0.42371689459806916 2110 44-45 -0.9462982526296102 2110 46-47 -0.12961913445985473 2110 48-49 0.018792344410513806 2110 50-51 0.09024618611367075 2110 52-53 0.09576570824143005 2110 54-55 -0.04887884034906875 2110 56-57 -0.14232647792254483 2110 58-59 -0.08998696070189638 2110 60-61 -0.035969584724032444 2110 62-63 -0.15547585994603708 2110 64-65 -0.15940620653565318 2110 66-67 -0.14017994699119996 2110 68-69 -0.1342953227608028 2110 70-71 -0.052727572639547304 2110 72-73 -0.07986790660802967 2110 74-75 -0.08718477727876461 2110 76-77 -0.11183004133649987 2110 78-79 -0.11207975705187678 2110 80-81 -0.06602879976930609 2110 82-83 -0.08196148729197716 2110 84-85 -0.14631058972877753 2110 86-87 -0.11383722268888619 2110 88-89 0.0011362479630783184 2110 90-91 -0.005148211185161955 2110 92-93 0.025207400025845317 2110 94-95 0.06961093735142398 2110 96-97 0.015202203963664118 2110 98-99 -0.019648179437037783 2110 100-101 -0.11504486668769331 2111 1 0.1836168195347554 2111 2 0.14051177347340982 2111 3 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-0.11683774047823192 2214 12-13 0.068381952464037 2214 14-15 -0.02881599725903783 2214 16-17 -0.14113514789988812 2214 18-19 -0.06415356385438287 2214 20-21 -0.0530305487674525 2214 22-23 -4.797526079599365 2214 24-25 -3.921088909676449 2214 26-27 -1.1623162203803936 2214 28-29 -0.5813333911174432 2214 30-31 -0.6268287287849041 2214 32-33 -1.0297157282702578 2214 34-35 -1.4001545505040696 2214 36-37 -0.7290713784291221 2214 38-39 -1.0315383741314683 2214 40-41 -0.8463586793817122 2214 42-43 -0.9079899898380503 2214 44-45 -0.38205345100384136 2214 46-47 -0.7691102593488992 2214 48-49 -0.7376261638853769 2214 50-51 -0.7413198824885221 2214 52-53 -0.3814455327554498 2214 54-55 -0.20737891676244402 2214 56-57 -0.23648747384076074 2214 58-59 -0.21925355612363973 2214 60-61 -0.11904684398424692 2214 62-63 -0.1277874507911605 2214 64-65 -0.21131726882528312 2214 66-67 -0.1464380057595065 2214 68-69 -0.20756634302214394 2214 70-71 -0.12815864436026914 2214 72-73 -0.09497835560673806 2214 74-75 -0.17834817920182644 2214 76-77 -0.1052315951082754 2214 78-79 -0.09103200325669292 2214 80-81 -0.0725206620360197 2214 82-83 -0.26057775758279433 2214 84-85 -0.2865228631961081 2214 86-87 -0.20046216113600934 2214 88-89 -0.12330997354616002 2214 90-91 -0.1399662791159848 2214 92-93 -0.29166711062363504 2214 94-95 -0.2859358663418483 2214 96-97 -0.2925643470811323 2214 98-99 -0.3004779610260364 2214 100-101 -0.28065671613713405 2215 1 -0.1506432593581799 2215 2 -0.1833201747200235 2215 3 -0.1186343070924778 2215 4 -0.15861198266157572 2215 5 -0.09169462394172356 2215 6 -0.40048816170329005 2215 7 -0.27640240108420144 2215 8 -0.27014998128041157 2215 9 -0.5914237128123574 2215 10-11 -0.3992131577670932 2215 12-13 -0.2616079668922211 2215 14-15 -0.24143284585627356 2215 16-17 -0.3121827839565512 2215 18-19 -0.1439730016838041 2215 20-21 -0.3909430061881167 2215 22-23 3.494440294902038 2215 24-25 2.4396096618693655 2215 26-27 0.29752150030777 2215 28-29 -0.3991631592022564 2215 30-31 -0.5954146080358278 2215 32-33 -0.3705092507444796 2215 34-35 -0.878847399999195 2215 36-37 -0.41758336742597635 2215 38-39 -0.4978579149593365 2215 40-41 -0.28109854132715384 2215 42-43 -0.34399615122806537 2215 44-45 -0.8455869676487637 2215 46-47 -0.32693457245465396 2215 48-49 -0.27365872893258114 2215 50-51 -0.03667382561550525 2215 52-53 -0.1711469947683284 2215 54-55 -0.18434489172033608 2215 56-57 -0.3013587430781399 2215 58-59 -0.20777392528225747 2215 60-61 -0.17753331387892146 2215 62-63 -0.1465443882841413 2215 64-65 -0.20419099673666707 2215 66-67 -0.13706865145620029 2215 68-69 -0.1198857386812513 2215 70-71 -0.014458466530463454 2215 72-73 -0.029896624949834916 2215 74-75 -0.017713401448069988 2215 76-77 -0.01564731355930249 2215 78-79 -0.0458018460437728 2215 80-81 -0.030729048626554345 2215 82-83 -0.1880148389393348 2215 84-85 -0.1779732269557961 2215 86-87 -0.2155102156053772 2215 88-89 -0.12459906152075817 2215 90-91 -0.16152422851763149 2215 92-93 -0.18399980550469053 2215 94-95 -0.2382692819936203 2215 96-97 -0.13705440710376493 2215 98-99 -0.20436893069308937 2215 100-101 -0.11879301445322099 2216 1 -0.7712712790875607 2216 2 -0.2165877352554304 2216 3 -0.012053129369601123 2216 4 0.04232158218929172 2216 5 0.061140435169388496 2216 6 0.04116396089140295 2216 7 0.22127268935286537 2216 8 0.2146904003926835 2216 9 -0.17679781823952823 2216 10-11 -0.16977587338327993 2216 12-13 0.06871609233663634 2216 14-15 -0.030171515986033626 2216 16-17 0.06605447027874334 2216 18-19 0.1470896978995455 2216 20-21 -0.006405441509620857 2216 22-23 3.8478599396042235 2216 24-25 2.6442049799591203 2216 26-27 0.568856209665551 2216 28-29 0.10551955268481095 2216 30-31 -0.08637216318284757 2216 32-33 -0.20558355699775888 2216 34-35 -0.3182470152790131 2216 36-37 -0.34687591305677756 2216 38-39 -0.191622724124489 2216 40-41 -0.1329857141936941 2216 42-43 -0.22475151493251033 2216 44-45 -0.08707300194647871 2216 46-47 -0.2711790836262331 2216 48-49 -0.1974240712531241 2216 50-51 -0.1538789622644643 2216 52-53 -0.20565954949628562 2216 54-55 -0.33177811538568136 2216 56-57 -0.26335151803787227 2216 58-59 -0.20924377150615925 2216 60-61 -0.10788899978397026 2216 62-63 -0.18346818812038634 2216 64-65 -0.233410743540027 2216 66-67 -0.24037640563093277 2216 68-69 -0.28843461991170116 2216 70-71 -0.255331817481256 2216 72-73 -0.1439608740346081 2216 74-75 -0.3440076308658959 2216 76-77 -0.3761171536754624 2216 78-79 -0.4455719826910318 2216 80-81 -0.28047803720065545 2216 82-83 -0.17177066080961367 2216 84-85 -0.20572435756758267 2216 86-87 -0.3135360200719717 2216 88-89 -0.42136369262812323 2216 90-91 -0.3159337502233761 2216 92-93 -0.3248816860934909 2216 94-95 -0.3431970735816883 2216 96-97 -0.35621049803725313 2216 98-99 -0.37515573911376876 2216 100-101 -0.24482418449051835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 268.0 3 244.0 4 300.0 5 277.0 6 316.0 7 680.0 8 2379.0 9 7667.0 10 23109.0 11 53827.0 12 84019.0 13 100066.0 14 105063.0 15 106626.0 16 109803.0 17 113662.0 18 118297.0 19 122681.0 20 128290.0 21 135862.0 22 146336.0 23 159021.0 24 173240.0 25 181572.0 26 182857.0 27 185935.0 28 199103.0 29 215322.0 30 234896.0 31 262821.0 32 302586.0 33 353509.0 34 420249.0 35 497749.0 36 504151.0 37 416596.0 38 181097.0 39 17735.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.295231426389876 48.16651898883638 21.22943248248315 17.308817102290597 2 17.326167869623358 49.46728483983827 16.17226665909599 17.034280631442382 3 43.57955711529779 22.72405902392973 16.885577857133086 16.810806003639396 4 17.025253554014817 22.617286636398042 16.737327088056926 43.62013272153021 5 17.22619800690314 48.95711539477755 17.651423698025287 16.165262900294024 6 43.14172718966541 23.773050853341363 16.40874814480785 16.67647381218538 7 17.31315760984172 23.048294458021147 43.367766216916735 16.2707817152204 8 42.39610554738207 24.074916156434114 16.914399443043752 16.61457885314007 9 42.18084623282768 23.863008370581813 17.307993932144903 16.648151464445597 10-11 15.937681253728755 36.803480105795956 30.63148788409544 16.62735075637985 12-13 15.759750204303351 24.15856779330829 30.25481220340096 29.826869798987392 14-15 16.07018465604692 49.646145318488884 17.48876737996861 16.794902645495586 16-17 29.270182318667942 23.148557582743752 17.48200906697702 30.099251031611278 18-19 16.38814026313661 35.91106856214773 17.71553794767264 29.985253227043014 20-21 29.734309251167844 35.68832981596922 17.81735820687368 16.760002725989253 22-23 30.989040175533116 21.096827960026555 29.051819351335816 18.86231251310451 24-25 24.75434057157723 29.560010217891836 22.01983075439962 23.665818456131312 26-27 26.74502902276219 26.261594327676768 22.331483681309653 24.66189296825139 28-29 42.69909880813309 21.41600420000811 18.824143135564704 17.060753856294102 30-31 35.14102725623467 25.57194403881644 20.78483224106714 18.502196463881745 32-33 25.01370808514023 29.40280131594196 24.42155130792366 21.161939290994145 34-35 23.27367739026587 27.853289175927987 23.886751375813798 24.986282057992344 36-37 25.90914125765264 26.26776064954699 25.299744038798327 22.52335405400204 38-39 26.00340577003174 25.90113172949457 24.369955212763962 23.725507287709725 40-41 22.583040252103757 28.47997490735279 25.676011898131108 23.26097294241234 42-43 23.114035181535407 26.99562316339491 25.841560311661908 24.04878134340777 44-45 22.908035078875454 29.522027991711962 23.393431685736367 24.176505243676218 46-47 24.89964902190853 25.987604080280786 23.92371425710493 25.189032640705754 48-49 23.814234246677685 27.529321676633465 23.906675200467735 24.74976887622112 50-51 24.48556207156916 28.812434912486523 23.58507479827738 23.11692821766694 52-53 25.14827790273873 25.718728875948532 25.41086413762886 23.72212908368388 54-55 24.560374910910934 27.169135416321875 24.84791460381591 23.422575068951282 56-57 24.855837709941856 26.949168503540733 23.629400853319986 24.56559293319743 58-59 27.2357064643299 25.773644651112182 23.8872374497636 23.10341143479432 60-61 24.964686175517727 26.55294216573721 23.876687623570582 24.605684035174477 62-63 25.205232039672833 27.482512074385195 23.938345523494835 23.37391036244714 64-65 23.54910700922198 26.320174282488225 25.98012090368672 24.150597804603073 66-67 25.06478281590903 26.961822786923555 25.198676425658167 22.774717971509244 68-69 26.9239519323336 26.38504491547516 24.040222083402043 22.650781068789204 70-71 23.111353399050376 27.106590303209106 26.040007439664958 23.742048858075567 72-73 24.006967821713385 26.137139011378295 24.879792675663207 24.976100491245113 74-75 24.32955727488543 28.170315085268122 24.592704884616573 22.907422755229877 76-77 24.922552578087313 25.42224398565449 25.124905748251347 24.53029768800685 78-79 23.87779863015128 26.46804994695501 24.100779161962627 25.55337226093109 80-81 25.029446558644064 26.66987559748376 24.97534289033667 23.325334953535503 82-83 25.079478859841924 26.202577684893164 25.420569843007495 23.297373612257417 84-85 26.89505787353052 26.06454776264734 23.7605861756319 23.27980818819024 86-87 24.458870331126178 26.374493004273518 24.472681258588537 24.693955406011764 88-89 26.22812174424991 25.56109439898522 23.785416531611748 24.425367325153125 90-91 24.93212750161996 26.642377646941927 23.615849953508942 24.809644897929168 92-93 24.317446234405345 27.79251415565885 25.181719761948756 22.70831984798705 94-95 23.478641854323005 27.025479831584065 25.629090764819058 23.866787549273873 96-97 25.23612395996937 25.9081384882012 24.69013513834778 24.165602413481647 98-99 26.58827914289663 26.028659855744564 24.304970189672233 23.07809081168658 100-101 23.804259086214525 28.07084649285056 24.79457040279349 23.330324018141425 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 43471.0 1 30938.5 2 13551.5 3 7309.5 4 5450.0 5 4725.5 6 4348.0 7 4159.0 8 4177.0 9 4235.0 10 4246.5 11 4917.0 12 17992.0 13 18863.5 14 6618.5 15 5934.0 16 6059.0 17 6340.5 18 7049.0 19 7899.5 20 8841.0 21 10408.5 22 12348.5 23 14610.0 24 17002.5 25 19732.5 26 23067.0 27 26606.0 28 30582.0 29 35337.5 30 40320.0 31 45120.0 32 50530.5 33 56637.0 34 63651.0 35 71204.5 36 78510.0 37 85365.5 38 91458.0 39 97080.5 40 104176.5 41 114514.0 42 125130.5 43 134344.0 44 144484.0 45 155438.5 46 166962.5 47 177508.0 48 187155.0 49 196382.0 50 206802.0 51 222252.5 52 245689.0 53 283585.0 54 290718.0 55 269139.0 56 258454.5 57 241998.5 58 216226.0 59 189512.5 60 163664.0 61 135952.0 62 111161.5 63 90389.0 64 73539.5 65 60043.0 66 48372.5 67 39031.0 68 30710.5 69 23507.0 70 17918.5 71 13760.0 72 10920.5 73 8601.0 74 6666.0 75 5090.0 76 3765.0 77 2694.5 78 1990.5 79 1507.5 80 1021.0 81 688.5 82 517.0 83 371.5 84 264.0 85 187.5 86 129.5 87 88.5 88 64.0 89 49.0 90 29.0 91 17.5 92 14.0 93 12.0 94 8.5 95 6.0 96 4.0 97 2.5 98 6.0 99 11.5 100 8.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0755272673612257 2 0.01877494669744755 3 0.018792045920304968 4 0.02120303634320124 5 0.01258502802306138 6 0.007557856502979796 7 0.02038227364604506 8 0.012345638903057496 9 0.028589900617606828 10-11 0.015825330754542538 12-13 0.026007917966136357 14-15 0.02027967830890054 16-17 0.020117235691755046 18-19 0.014329148754518257 20-21 0.013687927897364992 22-23 9.803275150947664 24-25 0.09449885512153358 26-27 0.052417667669422095 28-29 0.043021644709269606 30-31 0.04010622721207944 32-33 0.03790042746347221 34-35 0.03182165373765928 36-37 0.04456912437786615 38-39 0.053665910938013775 40-41 0.07311627693832944 42-43 0.07500574106407437 44-45 0.0706539888468609 46-47 0.10215075735022919 48-49 0.09525122092726007 50-51 0.09583259450441237 52-53 0.056119649418053606 54-55 0.03836210648062256 56-57 0.042123935509255035 58-59 0.050964233726541366 60-61 0.05349491870943957 62-63 0.06181369062957457 64-65 0.03553218509771949 66-67 0.04331233149784575 68-69 0.03637004701773309 70-71 0.020202731806042148 72-73 0.022938607463229402 74-75 0.028906236240469108 76-77 0.025828376126133444 78-79 0.022160592823216776 80-81 0.034796918514850415 82-83 0.026307154366141214 84-85 0.017603649931714253 86-87 0.023930362388959785 88-89 0.03132577627479409 90-91 0.014671133211666663 92-93 0.01406411080022824 94-95 0.011499227371615187 96-97 0.023870515108958813 98-99 0.03288180555481934 100-101 0.04243172152068861 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5848219.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.66287933900331 #Duplication Level Percentage of deduplicated Percentage of total 1 90.18242650139342 64.62732348867891 2 5.365240749946869 7.689772009762922 3 1.65734797345507 3.56310983533357 4 0.785418544591892 2.251414175668174 5 0.4836979741629274 1.7331594779479105 6 0.3073222485404298 1.3214158329206402 7 0.24011719966618583 1.2045242934828069 8 0.1789021922439798 1.0256516973010799 9 0.13459312643839502 0.8680797881832526 >10 0.6064649469651329 7.398612670181444 >50 0.03634370922391787 1.7535009937669963 >100 0.019812034119162526 2.6650515179186534 >500 0.0014868086645100857 0.7473451321965042 >1k 6.371927395377192E-4 0.5862968602968882 >5k 7.079919318198725E-5 0.3948333136604701 >10k+ 1.1799865530331208E-4 2.1699089126999462 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40495 0.6924330296112372 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA 35726 0.6108868358041996 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 29023 0.49627074499091095 No Hit AAAAAAAAAAAAAAAAAAAAAANAAAAAAAAAAAAAAAAAAAAAAAAAAA 12297 0.21026914347769807 No Hit AAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAA 10763 0.18403893561441526 No Hit AAGCAGTGGTATCAACGCAGAGNCACCGGGTTTCGGGTCTATACCCTGCA 9341 0.15972384071116352 No Hit AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA 7397 0.12648295147633834 No Hit AAGCAGTGGTATCAACGCAGAGNACTTTTTTTTTTTTTTTTTTTTTTTTT 6608 0.11299166464183369 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.7099222857420354E-5 0.0 0.0 0.0 0.0 6 1.7099222857420354E-5 0.0 0.0 0.0 1.7099222857420354E-5 7 3.419844571484071E-5 0.0 0.0 0.0 1.7099222857420354E-5 8 3.419844571484071E-5 0.0 0.0 0.0 1.7099222857420354E-5 9 3.419844571484071E-5 0.0 0.0 0.0 1.7099222857420354E-5 10-11 4.274805714355088E-5 1.7099222857420354E-5 0.0 0.0 2.5648834286130532E-5 12-13 5.129766857226106E-5 1.7099222857420354E-5 0.0 0.0 3.419844571484071E-5 14-15 5.129766857226106E-5 1.7099222857420354E-5 0.0 0.0 3.419844571484071E-5 16-17 7.694650285839158E-5 1.7099222857420354E-5 0.0 0.0 3.419844571484071E-5 18-19 9.404572571581195E-5 2.5648834286130532E-5 0.0 0.0 3.419844571484071E-5 20-21 1.1969456000194248E-4 3.419844571484071E-5 0.0 0.0 3.419844571484071E-5 22-23 1.3679378285936283E-4 3.419844571484071E-5 0.0 1.7099222857420354E-5 3.419844571484071E-5 24-25 1.6244261714549335E-4 3.419844571484071E-5 0.0 1.7099222857420354E-5 3.419844571484071E-5 26-27 2.3938912000388493E-4 3.419844571484071E-5 0.0 1.7099222857420354E-5 3.419844571484071E-5 28-29 2.8213717714743584E-4 3.419844571484071E-5 0.0 1.7099222857420354E-5 3.419844571484071E-5 30-31 3.0778601143356633E-4 4.274805714355088E-5 0.0 1.7099222857420354E-5 3.419844571484071E-5 32-33 3.9328212572066815E-4 6.839689142968141E-5 0.0 5.129766857226106E-5 3.419844571484071E-5 34-35 4.7022862857905973E-4 8.549611428710176E-5 0.0 5.129766857226106E-5 3.419844571484071E-5 36-37 4.958774628651902E-4 8.549611428710176E-5 0.0 5.129766857226106E-5 6.839689142968141E-5 38-39 5.728239657235819E-4 8.549611428710176E-5 0.0 5.129766857226106E-5 1.2824417143065265E-4 40-41 6.32671245724553E-4 8.549611428710176E-5 0.0 5.129766857226106E-5 1.4534339428807298E-4 42-43 7.267169714403651E-4 8.549611428710176E-5 0.0 5.129766857226106E-5 1.5389300571678317E-4 44-45 7.609154171552058E-4 8.549611428710176E-5 0.0 5.984728000097123E-5 1.7099222857420353E-4 46-47 8.549611428710176E-4 8.549611428710176E-5 0.0 6.839689142968141E-5 1.7099222857420353E-4 48-49 9.917549257303804E-4 8.549611428710176E-5 0.0 6.839689142968141E-5 1.880914514316239E-4 50-51 0.0011028998743036126 1.0259533714452211E-4 0.0 6.839689142968141E-5 1.880914514316239E-4 52-53 0.0011627471543045839 1.0259533714452211E-4 0.0 8.549611428710176E-5 1.880914514316239E-4 54-55 0.0012396936571629756 1.1969456000194248E-4 0.0 1.0259533714452211E-4 1.880914514316239E-4 56-57 0.001308090548592657 1.1969456000194248E-4 0.0 1.111449485732323E-4 1.880914514316239E-4 58-59 0.0014534339428807302 1.3679378285936283E-4 0.0 1.1969456000194248E-4 2.0519067428904423E-4 60-61 0.0015303804457391216 1.4534339428807298E-4 0.0 1.1969456000194248E-4 2.0519067428904423E-4 62-63 0.001607326948597513 1.5389300571678317E-4 0.0 1.1969456000194248E-4 2.222898971464646E-4 64-65 0.001744120731456876 1.5389300571678317E-4 0.0 1.1969456000194248E-4 2.222898971464646E-4 66-67 0.0019236625714597897 1.5389300571678317E-4 0.0 1.1969456000194248E-4 2.222898971464646E-4 68-69 0.002017708297175602 1.5389300571678317E-4 0.0 1.1969456000194248E-4 2.222898971464646E-4 70-71 0.002128853245748834 1.5389300571678317E-4 0.0 1.1969456000194248E-4 2.222898971464646E-4 72-73 0.002248547805750776 1.5389300571678317E-4 0.0 1.1969456000194248E-4 2.222898971464646E-4 74-75 0.002581982651470473 1.5389300571678317E-4 0.0 1.1969456000194248E-4 2.3083950857517477E-4 76-77 0.002881219051475329 1.5389300571678317E-4 0.0 1.1969456000194248E-4 2.3938912000388496E-4 78-79 0.003189005062908896 1.7099222857420353E-4 0.0 1.1969456000194248E-4 2.564883428613053E-4 80-81 0.003761829028632478 1.880914514316239E-4 0.0 1.1969456000194248E-4 2.564883428613053E-4 82-83 0.004625339782932205 1.880914514316239E-4 0.0 1.1969456000194248E-4 2.7358756571872566E-4 84-85 0.005599995485805165 1.880914514316239E-4 0.0 1.1969456000194248E-4 2.7358756571872566E-4 86-87 0.006890986811540403 1.880914514316239E-4 0.0 1.1969456000194248E-4 2.7358756571872566E-4 88-89 0.008626557931568569 1.880914514316239E-4 0.0 1.1969456000194248E-4 2.7358756571872566E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGTAT 191510 0.0 71.51652 6 TGGTATC 190675 0.0 71.37377 7 AGTGGTA 192370 0.0 71.252754 5 GTATCAA 192820 0.0 70.99181 9 GGTATCA 193145 0.0 70.83055 8 CAGTGGT 193570 0.0 70.704056 4 AAGCAGT 201315 0.0 68.717285 1 AGCAGTG 205940 0.0 67.11504 2 GCAGTGG 208095 0.0 66.38628 3 TCACCGG 7840 0.0 44.150192 22-23 GTCACCG 7995 0.0 43.403484 22-23 CTTAACG 9660 0.0 42.54936 52-53 TAACGCC 9730 0.0 42.36117 54-55 TCGGTTA 9725 0.0 41.1137 94-95 AGACTCG 9980 0.0 40.923553 66-67 CAGAGTA 45860 0.0 40.80656 18-19 TATACCC 8630 0.0 40.61936 40-41 CTATTCG 10185 0.0 40.456635 90-91 GGTCTAT 9800 0.0 40.396954 36-37 GTCTATA 9790 0.0 40.36647 36-37 >>END_MODULE