FastQCFastQC Report
Sat 18 Jun 2016
SRR799683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR799683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5848219
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGNAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT499240.8536616019338537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1786500.068.583929
GTGGTAT1790800.068.166846
GGTATCA1793800.068.118838
TGGTATC1796750.068.001837
AGTGGTA1827550.066.7009055
CAGTGGT1854900.066.324324
AAGCAGT1892300.065.1323241
AGCAGTG1924150.063.9517562
GCAGTGG1949200.063.1370123
AGAGTAC510200.045.45903818-19
AGTACTT425300.042.73801420-21
CTTAACG80900.042.6609852-53
GTACTTT419600.042.5691122-23
GAGTACT435700.042.4137920-21
ACGCAGA1525750.041.88001314-15
GCAGAGT1567000.041.69935216-17
CAACGCA1543000.041.56382812-13
TAACGCC83500.041.5050854-55
AACGCAG1534200.041.43570314-15
TCAACGC1549100.041.13033312-13
Produced by FastQC (version 0.11.5)