##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR799683_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5848219 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.336959508527297 31.0 31.0 34.0 30.0 34.0 2 31.601488247960617 31.0 31.0 34.0 30.0 34.0 3 31.791397688766445 31.0 31.0 34.0 30.0 34.0 4 35.292505974895946 37.0 35.0 37.0 33.0 37.0 5 35.218644000848805 37.0 35.0 37.0 33.0 37.0 6 34.93283476559274 36.0 35.0 37.0 32.0 37.0 7 35.04376939372482 36.0 35.0 37.0 32.0 37.0 8 34.77900297509378 36.0 35.0 37.0 32.0 37.0 9 36.24601079405542 38.0 35.0 39.0 32.0 39.0 10-11 36.43912232082964 38.0 35.0 39.0 32.0 39.0 12-13 36.33823921436594 38.0 35.0 39.0 32.0 39.0 14-15 37.64558740019825 39.0 37.0 40.0 32.5 41.0 16-17 36.008055871368704 39.0 36.0 40.0 27.0 41.0 18-19 31.81259662129616 38.0 28.5 40.0 10.0 41.0 20-21 36.212184769414414 38.0 34.0 40.0 29.5 41.0 22-23 37.048250330570724 39.0 36.0 40.0 32.0 41.0 24-25 36.10469127438627 38.5 35.0 40.0 28.5 41.0 26-27 34.9294462980952 38.0 33.0 40.0 25.0 41.0 28-29 34.66072294830272 38.0 33.0 40.0 24.0 41.0 30-31 34.32148171947733 38.0 32.5 40.0 23.5 41.0 32-33 34.146646697054265 38.0 32.0 40.0 23.0 41.0 34-35 34.27021491500233 38.0 32.0 40.0 23.0 41.0 36-37 34.20621021545192 37.5 32.0 40.0 23.0 41.0 38-39 33.76822713034515 37.0 31.5 40.0 23.0 41.0 40-41 33.70376092276982 37.0 31.0 40.0 23.0 41.0 42-43 33.61178813584102 36.5 31.5 40.0 21.5 41.0 44-45 32.92951734536617 36.0 31.0 40.0 18.0 41.0 46-47 33.163755238988145 36.0 31.0 39.5 20.0 41.0 48-49 33.08098029502657 36.0 31.0 39.0 20.0 41.0 50-51 31.51668422471867 35.0 29.0 39.0 11.5 41.0 52-53 31.663071783050533 35.0 28.0 39.0 14.0 40.5 54-55 31.731217828880894 35.0 28.5 39.0 15.0 40.0 56-57 31.04548076944451 35.0 28.0 39.0 11.0 40.0 58-59 31.210954822314278 35.0 28.0 38.0 13.0 40.0 60-61 31.250691962800985 34.5 28.0 38.0 14.5 40.0 62-63 30.736213965311492 34.0 28.0 37.5 12.0 40.0 64-65 30.729304938819837 34.0 28.0 37.0 13.0 40.0 66-67 30.552274034197417 34.0 28.0 36.5 13.5 39.0 68-69 30.273169917200434 34.0 27.5 36.0 13.0 39.0 70-71 29.964645561323884 34.0 26.5 35.5 13.0 39.0 72-73 29.750541917120408 33.0 26.0 35.0 13.0 38.0 74-75 29.56654897841548 33.0 26.0 35.0 13.0 37.0 76-77 29.356237941841783 33.0 26.0 35.0 12.5 37.0 78-79 29.236111455470457 33.0 26.0 35.0 12.0 36.0 80-81 29.01627085100609 33.0 26.0 35.0 11.0 36.0 82-83 29.005625986304548 33.0 26.0 35.0 11.0 36.0 84-85 28.76363308214005 33.0 26.0 35.0 10.5 35.0 86-87 28.590224647879978 33.0 26.0 35.0 10.0 35.0 88-89 28.538584994850567 33.0 25.0 35.0 10.0 35.0 90-91 28.489810829587608 33.0 25.0 35.0 10.0 35.0 92-93 28.454866088291155 33.0 25.0 35.0 10.0 35.0 94-95 28.44233372587449 33.0 25.0 35.0 10.0 35.0 96-97 28.29967679048955 33.0 25.0 35.0 10.0 35.0 98-99 28.18389145481727 32.5 25.0 35.0 10.0 35.0 100-101 26.547300468740996 30.5 22.0 34.0 8.5 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2104 1 -0.26328523806006743 2104 2 -0.05611127382649883 2104 3 -0.1293070208309821 2104 4 -0.12756844317658533 2104 5 -0.2098193147471008 2104 6 -0.1832274845070856 2104 7 -0.18554986502973492 2104 8 -0.005074464772455656 2104 9 -5.33343938513649 2104 10-11 -0.9047240931136855 2104 12-13 -0.08911878342215118 2104 14-15 -0.007631408821339392 2104 16-17 -2.143077507568151 2104 18-19 4.209300192021303 2104 20-21 0.7802691976029408 2104 22-23 -3.743593528895474 2104 24-25 -0.771082974301244 2104 26-27 -0.48330221468597045 2104 28-29 0.21580367348632024 2104 30-31 0.2620751389517437 2104 32-33 -0.29467507460212516 2104 34-35 0.0018599670427406068 2104 36-37 -0.04725823196977075 2104 38-39 0.12463654673801727 2104 40-41 -0.025978714539647285 2104 42-43 -0.008556367870724557 2104 44-45 0.6986279752724798 2104 46-47 0.021968813815739452 2104 48-49 -0.2199295297552908 2104 50-51 1.6397815540180254 2104 52-53 0.9548123164225082 2104 54-55 0.27437206125885893 2104 56-57 0.9611420155340547 2104 58-59 0.08655561709278459 2104 60-61 -0.1342596097405533 2104 62-63 -0.19461245518853332 2104 64-65 -0.4560520916043522 2104 66-67 -0.1505591188048676 2104 68-69 -0.36072401505401785 2104 70-71 -0.23506782902210333 2104 72-73 -0.2556578322157499 2104 74-75 -0.44197114229860546 2104 76-77 -0.4424579952798062 2104 78-79 -0.27093508914986586 2104 80-81 -0.29378277279724685 2104 82-83 -0.5155860462153647 2104 84-85 -0.5520098458617362 2104 86-87 -0.5709326095229308 2104 88-89 -0.5340217642319551 2104 90-91 -0.6952373102690217 2104 92-93 -0.7488355000785134 2104 94-95 -0.7569766441882173 2104 96-97 -0.6995393236867962 2104 98-99 -0.6246293706387291 2104 100-101 -0.688261769082807 2105 1 -0.05643420608164007 2105 2 0.05031943470083888 2105 3 0.04352668806164317 2105 4 -0.17686987155549616 2105 5 -0.07321136382435611 2105 6 0.10969918619201025 2105 7 -0.009406550768609634 2105 8 0.1799812875875446 2105 9 0.3861562870693689 2105 10-11 0.24225300281881346 2105 12-13 0.19981476225801487 2105 14-15 0.06870358519095277 2105 16-17 1.6296367344689315 2105 18-19 6.098212665813108 2105 20-21 1.3746583513740092 2105 22-23 0.4916934309616394 2105 24-25 0.14968037041553117 2105 26-27 0.328841421856211 2105 28-29 0.9728860000178017 2105 30-31 0.7798930942029045 2105 32-33 0.263241952911379 2105 34-35 0.3414420228302717 2105 36-37 0.4019052388736313 2105 38-39 0.3420038917793278 2105 40-41 0.36096133299984956 2105 42-43 0.28406077546198816 2105 44-45 1.258816683804696 2105 46-47 0.5244565241298105 2105 48-49 0.31491728401501007 2105 50-51 2.035899146733467 2105 52-53 1.44234793100431 2105 54-55 0.6426899526109686 2105 56-57 1.2884367576691602 2105 58-59 0.5027958620403226 2105 60-61 0.009040744194042105 2105 62-63 0.2725073245460301 2105 64-65 0.12481401132291836 2105 66-67 0.06088478827232535 2105 68-69 0.06536654331228675 2105 70-71 0.04075515858426826 2105 72-73 0.16873363376087624 2105 74-75 0.06441245155831155 2105 76-77 0.033797238087547754 2105 78-79 0.06252495409255943 2105 80-81 0.08865398019756654 2105 82-83 0.06997905299183671 2105 84-85 0.016069634114874276 2105 86-87 0.08061727335202207 2105 88-89 -0.09431489635465695 2105 90-91 -0.17452322179860857 2105 92-93 -0.16452905553679642 2105 94-95 -0.14819629605502982 2105 96-97 -0.16863022383468618 2105 98-99 -0.14731412247883213 2105 100-101 -0.2769548532202748 2106 1 -0.11649744773767878 2106 2 -0.04389283651198994 2106 3 -0.03883003278901498 2106 4 -0.09564080375093909 2106 5 -0.13100945231007444 2106 6 -0.13172897782508386 2106 7 -0.13699514208177987 2106 8 0.09725926225195991 2106 9 0.3989801811436351 2106 10-11 0.2070580171435168 2106 12-13 -0.0037049411859726433 2106 14-15 -0.06867369120765687 2106 16-17 0.854223903848407 2106 18-19 5.81758387237911 2106 20-21 1.141242375646165 2106 22-23 0.32562549244445194 2106 24-25 -0.19020045470353608 2106 26-27 -0.5395993411916677 2106 28-29 0.2630131913569116 2106 30-31 0.1827186735974351 2106 32-33 -0.13730209945603633 2106 34-35 -0.31364664470994086 2106 36-37 -0.19358142896306418 2106 38-39 -0.05979154926023966 2106 40-41 -1.9415607931375476 2106 42-43 -1.777769580949169 2106 44-45 -0.17244236295444182 2106 46-47 -0.10244790988588903 2106 48-49 -0.26666068840162893 2106 50-51 1.618098737095007 2106 52-53 1.1653589792698718 2106 54-55 0.38727216046793345 2106 56-57 1.1740464315115595 2106 58-59 -0.9014357910059871 2106 60-61 -0.706494466095581 2106 62-63 0.23123429493595538 2106 64-65 0.04421272726913372 2106 66-67 -0.7568739156732036 2106 68-69 -1.2749294590757785 2106 70-71 -1.8542159548754569 2106 72-73 -2.621676852942862 2106 74-75 -1.2016413231662462 2106 76-77 -0.6125261673270899 2106 78-79 -0.4791100076708581 2106 80-81 -0.29069578770537063 2106 82-83 -0.07441578200885957 2106 84-85 -1.161396645301025 2106 86-87 -1.4092941219365436 2106 88-89 -0.8242265480314757 2106 90-91 -0.40617525347846595 2106 92-93 -0.243339618514419 2106 94-95 -0.31977800971094794 2106 96-97 -0.09635713537628732 2106 98-99 -0.14447460953378766 2106 100-101 -0.33766298418552765 2107 1 -0.009642316661903294 2107 2 -0.14944949840642963 2107 3 -0.007672987211410742 2107 4 -0.1040270203991156 2107 5 0.05605080374216698 2107 6 0.06304888327210278 2107 7 0.03128511226944397 2107 8 0.2786240111524023 2107 9 0.41564938376536986 2107 10-11 0.35423891269657304 2107 12-13 0.2483824200162772 2107 14-15 0.11048083530597808 2107 16-17 1.6954415453740808 2107 18-19 6.1100352712564785 2107 20-21 1.4084745632374123 2107 22-23 0.4267104442179033 2107 24-25 0.1286043746943264 2107 26-27 0.35670516158709376 2107 28-29 0.8742978604026064 2107 30-31 0.876260923050495 2107 32-33 0.3957778398597682 2107 34-35 0.3931083395277213 2107 36-37 0.35616611348778093 2107 38-39 0.2763555559164672 2107 40-41 0.28039129355507697 2107 42-43 0.21775684449750798 2107 44-45 1.2106890880100707 2107 46-47 0.4692113866797527 2107 48-49 0.20723095580206774 2107 50-51 2.0495917082407082 2107 52-53 1.415983055472271 2107 54-55 0.6143500164997526 2107 56-57 1.2908369916695257 2107 58-59 0.6018474612543088 2107 60-61 0.10948326567411826 2107 62-63 0.4112513798088635 2107 64-65 0.052141206153173414 2107 66-67 -0.006451750950944302 2107 68-69 0.05409841916600655 2107 70-71 0.2019961174599878 2107 72-73 0.3798546539258254 2107 74-75 0.2075646686796695 2107 76-77 0.09573851646279508 2107 78-79 0.21406740835427485 2107 80-81 0.19487377750004597 2107 82-83 0.11545496841096181 2107 84-85 0.19771200060749905 2107 86-87 0.24064288543668155 2107 88-89 0.006290697637272302 2107 90-91 0.003046458475886027 2107 92-93 -0.0278850588878079 2107 94-95 -6.711193780795099E-4 2107 96-97 -1.1690140177244785 2107 98-99 -1.0991049177549996 2107 100-101 -0.49241712211483346 2108 1 0.04669907606586321 2108 2 0.15549890161972613 2108 3 0.11848661076143685 2108 4 0.10252027831094068 2108 5 -0.01945386033614227 2108 6 0.14573490056806548 2108 7 0.11748954478104423 2108 8 0.23137134094546496 2108 9 0.5645102205598249 2108 10-11 0.3531135571629278 2108 12-13 0.19833378951447145 2108 14-15 0.14871325258097556 2108 16-17 -1.9579859417627148 2108 18-19 5.131911028164836 2108 20-21 1.0420963281158464 2108 22-23 0.33111889253402893 2108 24-25 -0.1887579584072725 2108 26-27 -0.26771085773906833 2108 28-29 -3.5098622642396293 2108 30-31 -1.9916258233140383 2108 32-33 -0.8327808675576875 2108 34-35 -0.34462465063998593 2108 36-37 -0.21690751476523928 2108 38-39 -0.05452881614252192 2108 40-41 -0.0737476057898192 2108 42-43 -0.15847129951189487 2108 44-45 0.9667808907236122 2108 46-47 0.20457543487558638 2108 48-49 0.01161288167023855 2108 50-51 1.8140858587954476 2108 52-53 1.0329939042158038 2108 54-55 0.5514998907696373 2108 56-57 1.2514842124225254 2108 58-59 0.6349811615951566 2108 60-61 -0.08703485436790359 2108 62-63 0.3757666782347435 2108 64-65 0.06550638061274583 2108 66-67 0.05455135632259811 2108 68-69 0.16657537080585172 2108 70-71 0.14133800627807602 2108 72-73 0.1705371730743792 2108 74-75 0.047508820172577515 2108 76-77 0.07411354765650202 2108 78-79 0.11404325578400787 2108 80-81 0.20668564238352616 2108 82-83 0.12984451409336017 2108 84-85 0.04361675838495671 2108 86-87 0.13575748990855985 2108 88-89 0.010183599396736298 2108 90-91 -0.0375313824105632 2108 92-93 -0.028829257912814654 2108 94-95 0.015894102457028936 2108 96-97 -0.010373689517638951 2108 98-99 -0.04303228444696572 2108 100-101 -0.05864091093119583 2109 1 0.1447807718456211 2109 2 0.21244300749593137 2109 3 0.1427551601226824 2109 4 0.18070900611510154 2109 5 0.20129556740266707 2109 6 0.19209289051478606 2109 7 0.15475687464446253 2109 8 0.2420110739723782 2109 9 0.4130263499196687 2109 10-11 0.24241031629122034 2109 12-13 0.20780294857338788 2109 14-15 0.09994483987112801 2109 16-17 -1.88958801155038 2109 18-19 5.229633475416854 2109 20-21 1.2902481453697732 2109 22-23 0.5775381461868321 2109 24-25 0.13830186397247246 2109 26-27 -0.20753538425841356 2109 28-29 0.14752772649639923 2109 30-31 0.0775389804574047 2109 32-33 -0.145980839114074 2109 34-35 -0.03876852115063656 2109 36-37 0.041037074037511445 2109 38-39 0.0492414859760828 2109 40-41 0.020258311329705236 2109 42-43 0.05259958997712033 2109 44-45 0.9187469472345455 2109 46-47 0.2880333759427103 2109 48-49 0.02568472033560454 2109 50-51 1.8762726139469414 2109 52-53 1.1776381827241558 2109 54-55 0.6163788321212458 2109 56-57 1.3270259221222318 2109 58-59 0.6863864664176731 2109 60-61 0.2625692746703301 2109 62-63 0.552880755899789 2109 64-65 0.326739896208494 2109 66-67 0.2706883802420208 2109 68-69 0.24279212139266804 2109 70-71 0.37034581793048105 2109 72-73 0.3525231512950313 2109 74-75 0.343347601200211 2109 76-77 0.210786434636276 2109 78-79 0.297145212631051 2109 80-81 0.32627673235856136 2109 82-83 0.22135218192058304 2109 84-85 0.2619318313156249 2109 86-87 0.23372364224083597 2109 88-89 0.2622841866087633 2109 90-91 0.23956489819794058 2109 92-93 0.20911370211337754 2109 94-95 0.09599699208318313 2109 96-97 0.15567467636506294 2109 98-99 0.11464599135388553 2109 100-101 -0.0018383127333017057 2110 1 0.08660428957827904 2110 2 0.11769569981893468 2110 3 0.10478507889758149 2110 4 0.12557408871532516 2110 5 0.1396175190886595 2110 6 0.17844828853260708 2110 7 0.17681804028228498 2110 8 -0.11928565638618949 2110 9 -0.065706964596977 2110 10-11 -4.378697009468588 2110 12-13 -2.9435111353322156 2110 14-15 -1.160198695888937 2110 16-17 0.7375589953406347 2110 18-19 5.626142113619579 2110 20-21 0.7136189988659609 2110 22-23 0.35822508818616683 2110 24-25 0.2121899003828247 2110 26-27 0.14912196258737254 2110 28-29 0.4755605188409149 2110 30-31 0.4237060608443102 2110 32-33 0.25570947116074194 2110 34-35 0.23034027120278466 2110 36-37 0.2853383575568458 2110 38-39 0.21993861235226575 2110 40-41 0.2411567435487072 2110 42-43 -0.28580675076098316 2110 44-45 0.7968301827156807 2110 46-47 0.4697110846169039 2110 48-49 0.12924373007356849 2110 50-51 1.7221241213726124 2110 52-53 1.5333564555930685 2110 54-55 0.92630903613939 2110 56-57 1.5660675820166787 2110 58-59 0.9240463402679779 2110 60-61 0.5339703557486786 2110 62-63 0.7432423922097406 2110 64-65 0.4299518018847408 2110 66-67 0.3741029346631919 2110 68-69 0.34880679925243285 2110 70-71 0.45250565856955305 2110 72-73 0.5768262046750294 2110 74-75 0.3866982816571358 2110 76-77 0.2401088495933834 2110 78-79 0.3234757260104786 2110 80-81 0.27487000025181274 2110 82-83 0.16472830826466378 2110 84-85 0.25762766146006655 2110 86-87 0.3051739710922696 2110 88-89 0.25345627035984464 2110 90-91 0.14188125076502445 2110 92-93 0.08275994388140617 2110 94-95 0.0973074898981956 2110 96-97 0.012250973348042038 2110 98-99 0.04919744729763309 2110 100-101 -0.02728764747585899 2111 1 0.030601895486796593 2111 2 0.08995112639777503 2111 3 0.0456115011209306 2111 4 0.1930404373742789 2111 5 0.20873998114927161 2111 6 -0.3091876356214698 2111 7 -0.4500302132970475 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26-27 -0.21351945719285226 2214 28-29 0.2014905262825053 2214 30-31 0.18866751346465094 2214 32-33 -0.09413975434640065 2214 34-35 -0.2796175022894829 2214 36-37 -0.3136973544218833 2214 38-39 -0.30465333188148236 2214 40-41 -0.043974216017531376 2214 42-43 -0.037338639413974306 2214 44-45 0.8994015994442606 2214 46-47 0.21478292137020816 2214 48-49 -0.050275813943642333 2214 50-51 -0.34774812245080966 2214 52-53 -0.38220268220899456 2214 54-55 0.08475250136871892 2214 56-57 1.0906286353069206 2214 58-59 0.48336466144515455 2214 60-61 0.12358163073555417 2214 62-63 0.29392330124732524 2214 64-65 0.12640353899040946 2214 66-67 0.05072882003219803 2214 68-69 0.09714218970452748 2214 70-71 0.15313312716900285 2214 72-73 0.15991563406610965 2214 74-75 0.060315124942814435 2214 76-77 -0.049938001024230516 2214 78-79 -0.10583830660422322 2214 80-81 -0.12342873050678449 2214 82-83 -0.04301109363734312 2214 84-85 -0.01567246319520521 2214 86-87 0.06480553227358854 2214 88-89 0.009501566932389238 2214 90-91 -0.0742884213734385 2214 92-93 -0.008212035750311486 2214 94-95 -0.04161383870827606 2214 96-97 0.03417006903747577 2214 98-99 0.05807984278832379 2214 100-101 0.06104101650745264 2215 1 0.02697164185757117 2215 2 0.021030283747336398 2215 3 -0.09720364984328711 2215 4 0.03379062645831965 2215 5 0.12020909524326129 2215 6 -0.0758703914804002 2215 7 -0.13006993570944303 2215 8 -0.263429059881652 2215 9 0.07902528319618085 2215 10-11 0.03039913921119819 2215 12-13 -0.2389551100532401 2215 14-15 -0.20841333178110943 2215 16-17 1.2564018518601188 2215 18-19 5.744289426029667 2215 20-21 1.2664032240319543 2215 22-23 0.10508171781209086 2215 24-25 -0.38591403188762996 2215 26-27 -0.5348619529068444 2215 28-29 0.009561752415272906 2215 30-31 -0.14673637281238427 2215 32-33 -0.6386017670558601 2215 34-35 -0.5819309448172376 2215 36-37 -0.6201016833428099 2215 38-39 -0.6061105945220504 2215 40-41 -0.764973937327035 2215 42-43 -0.7762723877863777 2215 44-45 -0.13371732688491988 2215 46-47 -0.42704505874242926 2215 48-49 -0.6305733344177042 2215 50-51 -6.643870733246853 2215 52-53 -7.504233006641851 2215 54-55 -2.0749806145588323 2215 56-57 0.15907563839873262 2215 58-59 -0.19616319223684897 2215 60-61 -0.4971167202351907 2215 62-63 -0.2186736413390662 2215 64-65 -0.6012015135788218 2215 66-67 -0.43421720597650904 2215 68-69 -0.555931442919551 2215 70-71 -0.7438528113303384 2215 72-73 -0.6797457184076592 2215 74-75 -0.6473257458688373 2215 76-77 -0.6168050008357078 2215 78-79 -0.7045656301760843 2215 80-81 -0.610156223471563 2215 82-83 -0.5126067705786816 2215 84-85 -0.5318803141557602 2215 86-87 -0.5442775003645899 2215 88-89 -0.7181768556494035 2215 90-91 -0.5693516986231373 2215 92-93 -0.48506723787796346 2215 94-95 -0.2789879044683836 2215 96-97 -0.45718431726185926 2215 98-99 -0.2759463793497794 2215 100-101 -0.06134329639340663 2216 1 -0.05400815184988872 2216 2 0.013123232964474596 2216 3 -0.08269334013641583 2216 4 0.05236095478703362 2216 5 0.13560759433235603 2216 6 0.08171214708238494 2216 7 0.010389343942904361 2216 8 0.16413289387795515 2216 9 0.31192948793871267 2216 10-11 0.16564192596227656 2216 12-13 -0.5232426112790307 2216 14-15 -0.36492262471780634 2216 16-17 1.0733470190545944 2216 18-19 5.517746089615308 2216 20-21 1.2314452491470078 2216 22-23 0.28558179466789824 2216 24-25 -0.4023472214513859 2216 26-27 -0.459756369476203 2216 28-29 0.2226156635448433 2216 30-31 0.12544481086554526 2216 32-33 -0.3335640126978987 2216 34-35 -0.6260240835441877 2216 36-37 -0.5972090532045442 2216 38-39 -0.6216751161650222 2216 40-41 -0.6784527101076563 2216 42-43 -0.7060964876487219 2216 44-45 0.3421796381417863 2216 46-47 -0.3011032675201406 2216 48-49 -0.5784600561592868 2216 50-51 1.2631291517239127 2216 52-53 0.76264485021429 2216 54-55 0.1882329865788357 2216 56-57 0.8398402994510263 2216 58-59 0.11613218764294331 2216 60-61 -0.3523108456346975 2216 62-63 -0.10678681457817518 2216 64-65 -0.2558521640368845 2216 66-67 -0.430453073753295 2216 68-69 -0.517839133573716 2216 70-71 -0.5352113655011337 2216 72-73 -0.43257587435540046 2216 74-75 -0.28395972039681894 2216 76-77 -0.41382443950324443 2216 78-79 -0.39569035520023377 2216 80-81 -0.3774208724780195 2216 82-83 -0.5180068139945853 2216 84-85 -0.5721057964726484 2216 86-87 -0.468197423817557 2216 88-89 -0.2771905834888848 2216 90-91 -0.27038985863728016 2216 92-93 -0.21916606910972547 2216 94-95 -0.3283208961072184 2216 96-97 -0.18354336090069623 2216 98-99 -0.2542168932352311 2216 100-101 -0.06707762230345082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 7.0 7 115.0 8 348.0 9 631.0 10 960.0 11 1643.0 12 2841.0 13 5159.0 14 9494.0 15 15549.0 16 25862.0 17 40144.0 18 53478.0 19 63359.0 20 73930.0 21 86554.0 22 102230.0 23 121877.0 24 144832.0 25 165153.0 26 179404.0 27 189891.0 28 204857.0 29 227980.0 30 261834.0 31 304347.0 32 358953.0 33 421087.0 34 496994.0 35 620133.0 36 703662.0 37 635864.0 38 295798.0 39 33205.0 40 44.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.069308041516962 40.97243125429276 22.29819587840445 19.66006482578583 2 18.19578514271957 42.699937433922166 18.833894159536147 20.27038326382212 3 41.933262075171946 20.20926370917368 18.672265864188738 19.185208351465636 4 19.444723256772704 19.72258562820578 18.765969605447403 42.06672150957411 5 19.4801943174502 42.34217012463329 20.3880324930321 17.789603064884407 6 42.07251573585312 20.20334774824687 18.89919991245035 18.82493660344966 7 19.08483591329258 20.181409075138944 42.65360103648649 18.080153975081988 8 41.761141297889154 20.669677383832582 19.35440516163981 18.214776156638457 9 41.17903245415399 20.456090991120547 19.884036490425547 18.480840064299915 10-11 18.046814574137446 31.75055014934397 31.5566655962784 18.64596968024018 12-13 18.031284396155478 20.51160361812716 31.44369251561886 30.0134194700985 14-15 18.02266638783534 43.27046405067936 20.188941282807637 18.517928278677662 16-17 29.71188172902699 20.02850858875947 20.1696216565532 30.089988025660347 18-19 17.98426216798209 32.802284325752794 20.141465570833244 29.07198793543187 20-21 29.584617806129444 31.559963623097744 20.303540404155697 18.551878166617122 22-23 29.536193760218925 20.04479826162431 31.835278712250464 18.5837292659063 24-25 22.99800084145398 28.15657883723577 22.715264780568347 26.130155540741907 26-27 24.247241311886185 22.7700958866784 29.493910567951144 23.488752233484274 28-29 34.03609940888787 19.959785428809795 27.60087884518279 18.403236317119543 30-31 29.047430995288355 22.63737396804248 28.91721931110239 19.397975725566777 32-33 23.201970805553263 24.679493591752767 31.211394955034628 20.907140647659343 34-35 22.228343792155258 23.973076719857637 30.389533620157955 23.40904586782915 36-37 24.167959443914132 22.857555682755244 31.42632409387679 21.54816077945384 38-39 24.08293829097176 22.819922754491326 30.641812812248475 22.45532614228844 40-41 21.49014087083994 24.76354736281515 31.732809567319354 22.013502199025556 42-43 21.816651260042946 23.62029495220185 31.79656109111043 22.76649269664478 44-45 21.745889062347086 25.287169597707766 29.87338988903993 23.093551450905224 46-47 23.21538298829371 22.75581464840739 30.32734668451521 23.701455678783688 48-49 22.463038545108084 24.05100076314249 30.03745434268774 23.448506349061685 50-51 22.97226538467835 25.22241574628426 29.67472957947149 22.1305892895659 52-53 23.394940424154882 22.993613011143992 30.84011495810476 22.77133160659637 54-55 23.05128887548542 24.547290207325865 29.557486356351635 22.843934560837077 56-57 23.676153812174068 24.70422742436295 27.84300453597949 23.776614227483492 58-59 25.825958029918684 23.91203996818373 27.50569152292046 22.756310478977127 60-61 23.82999941519208 24.801045609203985 27.394531943337242 23.974423032266692 62-63 24.11815146144803 25.627422397914863 27.47747141919627 22.776954721440838 64-65 22.69208135776733 24.675360539282178 29.18876919526861 23.44378890768189 66-67 23.652440170246702 25.121502802819112 28.786182596787157 22.439874430147025 68-69 25.09935096346624 24.445287247869306 28.12985244879328 22.32550933987118 70-71 22.061007868433492 24.770898381008102 29.964050806460783 23.204042944097623 72-73 22.888673777649192 23.910195674280544 29.08269761806196 24.11843293000831 74-75 23.131174883543093 25.584541677396672 28.78511020528768 22.499173233772556 76-77 23.538862882110408 23.61983006979423 29.10686608591448 23.734440962180887 78-79 22.671794264540104 24.662879391870085 27.89363369337951 24.7716926502103 80-81 23.591422525834325 24.95614660188638 28.33268512580456 23.119745746474734 82-83 23.651229596603258 24.433495355267667 28.77039142699313 23.144883621135943 84-85 25.030022545764822 24.67600188437109 27.185840541508743 23.10813502835534 86-87 23.503071880113744 24.757088066263012 27.620502683727153 24.119337369896087 88-89 24.79096951364439 24.266841535586224 26.8625591849786 24.079629765790784 90-91 23.638092840937617 25.202635137399636 26.677654100512367 24.481617921150388 92-93 23.370166199316408 26.091755455806286 27.770189522656384 22.767888822220918 94-95 22.75547100149482 25.360418713593486 28.116053868217183 23.76805641669451 96-97 24.22292154243882 24.47180928074 27.45795771327989 23.847311463541292 98-99 25.334085016069867 24.5042245390557 27.135652743094813 23.026037701779618 100-101 22.869973358744613 25.936290997077947 27.852669944294934 23.341065699882506 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 26044.0 1 16955.0 2 5617.0 3 2777.0 4 1890.5 5 1453.5 6 1314.5 7 1291.0 8 1159.5 9 1178.0 10 1785.5 11 13182.5 12 51163.0 13 51237.0 14 21245.5 15 17495.5 16 16788.0 17 17074.5 18 17704.5 19 18428.0 20 19076.5 21 19728.0 22 20718.0 23 22049.0 24 23612.0 25 25299.5 26 27712.5 27 30952.0 28 34028.5 29 37389.5 30 41700.0 31 46509.5 32 51659.5 33 57585.0 34 64639.5 35 71696.5 36 78561.0 37 85299.5 38 91333.0 39 96850.0 40 103493.0 41 113899.5 42 124721.0 43 134637.5 44 145817.0 45 158122.5 46 172797.5 47 187587.0 48 199878.5 49 207618.0 50 216163.0 51 231729.0 52 258183.5 53 294624.5 54 300844.5 55 278757.0 56 256429.0 57 227119.5 58 194989.5 59 165101.5 60 136733.5 61 110373.0 62 88552.0 63 70068.5 64 55715.0 65 44450.0 66 35112.0 67 28343.5 68 22019.0 69 16208.0 70 12034.0 71 9574.5 72 7989.5 73 6243.5 74 4632.0 75 3329.5 76 2214.0 77 1299.5 78 723.5 79 383.0 80 206.0 81 125.0 82 76.0 83 44.5 84 30.5 85 24.0 86 14.5 87 11.0 88 7.5 89 4.5 90 3.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.01988639618317987 2 3.419844571484071E-5 3 0.0 4 0.0 5 0.08544481661852951 6 0.0018638152914588185 7 0.0 8 0.0 9 0.0 10-11 0.05109247789797202 12-13 0.0 14-15 0.0 16-17 1.9400094285114835 18-19 9.575675945103972 20-21 4.274805714355088E-5 22-23 1.7099222857420354E-5 24-25 1.45343394288073E-4 26-27 2.3083950857517477E-4 28-29 0.0015389300571678317 30-31 8.464115314423075E-4 32-33 8.891595885858584E-4 34-35 7.865642514413363E-4 36-37 3.676332914345376E-4 38-39 0.0019151129600310797 40-41 0.0010345029828739314 42-43 0.00145343394288073 44-45 0.8466081725051678 46-47 0.002094654800033993 48-49 5.471751314374513E-4 50-51 4.431990320471925 52-53 2.5648834286130532E-5 54-55 0.8331681833392354 56-57 1.6790239900386768 58-59 0.004574042114359945 60-61 0.002479387314325951 62-63 0.8326808554877989 64-65 8.549611428710177E-6 66-67 0.0 68-69 2.0519067428904426E-4 70-71 0.0017868687886004268 72-73 0.001402136274308469 74-75 0.0022741966400369068 76-77 0.0029239671086188804 78-79 0.0021630516914636745 80-81 6.925185257255242E-4 82-83 0.0024366392571824004 84-85 0.0019493114057459202 86-87 1.538930057167832E-4 88-89 4.958774628651902E-4 90-91 3.9328212572066815E-4 92-93 0.0 94-95 3.590836800058274E-4 96-97 0.0 98-99 1.1969456000194248E-4 100-101 0.15974948954544965 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5848219.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.95838218107575 #Duplication Level Percentage of deduplicated Percentage of total 1 96.20247743926521 87.50421709687004 2 2.6134775380276176 4.7543537745114595 3 0.5840860350100264 1.5938256239721356 4 0.21362873836795493 0.7772529771733394 5 0.10978720291492217 0.4993033180663402 6 0.06398687218581486 0.34920854249093963 7 0.04268492939553805 0.27177864849321093 8 0.03109770252073 0.22628773686671697 9 0.02714061461668366 0.22217997572402426 >10 0.10015957167046473 1.5272276182188598 >50 0.00872905353176128 0.5546348713737722 >100 0.0025286345340141325 0.3380429359609974 >500 1.0783427022931042E-4 0.06434326137968718 >1k 4.313347561730632E-5 0.11895600716558599 >5k 4.313347561413613E-5 0.21904069524653702 >10k+ 2.1566737807068065E-5 0.9793469164864871 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGNAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 49924 0.8536616019338537 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 1.7099222857420354E-5 0.0 0.0 0.0 0.0 4 1.7099222857420354E-5 0.0 0.0 0.0 0.0 5 1.7099222857420354E-5 0.0 0.0 0.0 0.0 6 1.7099222857420354E-5 0.0 0.0 0.0 0.0 7 1.7099222857420354E-5 0.0 0.0 0.0 0.0 8 1.7099222857420354E-5 0.0 0.0 0.0 0.0 9 1.7099222857420354E-5 0.0 0.0 0.0 0.0 10-11 3.419844571484071E-5 0.0 0.0 0.0 0.0 12-13 3.419844571484071E-5 1.7099222857420354E-5 0.0 0.0 0.0 14-15 3.419844571484071E-5 1.7099222857420354E-5 0.0 0.0 0.0 16-17 7.694650285839158E-5 1.7099222857420354E-5 0.0 0.0 1.7099222857420354E-5 18-19 9.404572571581195E-5 1.7099222857420354E-5 0.0 0.0 1.7099222857420354E-5 20-21 1.1969456000194248E-4 1.7099222857420354E-5 0.0 0.0 1.7099222857420354E-5 22-23 1.5389300571678317E-4 1.7099222857420354E-5 0.0 0.0 1.7099222857420354E-5 24-25 2.137402857177544E-4 1.7099222857420354E-5 0.0 0.0 1.7099222857420354E-5 26-27 2.8213717714743584E-4 1.7099222857420354E-5 0.0 0.0 1.7099222857420354E-5 28-29 3.163356228622765E-4 1.7099222857420354E-5 0.0 0.0 1.7099222857420354E-5 30-31 3.4198445714840706E-4 1.7099222857420354E-5 0.0 0.0 3.419844571484071E-5 32-33 4.1038134857808846E-4 1.7099222857420354E-5 0.0 0.0 3.419844571484071E-5 34-35 4.7022862857905973E-4 2.5648834286130532E-5 0.0 0.0 5.129766857226106E-5 36-37 4.958774628651902E-4 4.274805714355088E-5 0.0 0.0 5.129766857226106E-5 38-39 5.557247428661614E-4 5.129766857226106E-5 0.0 0.0 9.404572571581195E-5 40-41 6.32671245724553E-4 5.129766857226106E-5 0.0 0.0 1.0259533714452211E-4 42-43 7.096177485829446E-4 5.129766857226106E-5 0.0 0.0 1.0259533714452211E-4 44-45 7.438161942977853E-4 5.129766857226106E-5 0.0 0.0 1.111449485732323E-4 46-47 8.036634742987566E-4 5.129766857226106E-5 0.0 0.0 1.1969456000194248E-4 48-49 8.891595885858584E-4 5.129766857226106E-5 0.0 0.0 1.1969456000194248E-4 50-51 9.319076457294092E-4 6.839689142968141E-5 0.0 0.0 1.1969456000194248E-4 52-53 9.490068685868297E-4 6.839689142968141E-5 0.0 0.0 1.3679378285936283E-4 54-55 0.0010516022057313517 6.839689142968141E-5 0.0 0.0 1.5389300571678317E-4 56-57 0.0010858006514461924 6.839689142968141E-5 0.0 0.0 1.5389300571678317E-4 58-59 0.0012225944343055553 6.839689142968141E-5 0.0 0.0 1.6244261714549335E-4 60-61 0.0013422889943074977 8.549611428710176E-5 0.0 0.0 1.7099222857420353E-4 62-63 0.0014106858857371792 8.549611428710176E-5 0.0 0.0 1.7099222857420353E-4 64-65 0.0014876323885955707 1.0259533714452211E-4 0.0 0.0 1.795418400029137E-4 66-67 0.0016158765600262233 1.0259533714452211E-4 0.0 8.549611428710177E-6 1.9664106286033407E-4 68-69 0.0017355711200281658 1.0259533714452211E-4 0.0 1.7099222857420354E-5 2.0519067428904423E-4 70-71 0.0018638152914588185 1.0259533714452211E-4 0.0 1.7099222857420354E-5 2.137402857177544E-4 72-73 0.001992059462889471 1.111449485732323E-4 0.0 1.7099222857420354E-5 2.564883428613053E-4 74-75 0.0023511431428952984 1.1969456000194248E-4 0.0 1.7099222857420354E-5 2.564883428613053E-4 76-77 0.002650379542900155 1.1969456000194248E-4 0.0 1.7099222857420354E-5 2.7358756571872566E-4 78-79 0.0029667151657624315 1.1969456000194248E-4 0.0 1.7099222857420354E-5 2.992364000048562E-4 80-81 0.0035138902971998823 1.1969456000194248E-4 0.0 1.7099222857420354E-5 3.0778601143356633E-4 82-83 0.004300454548641219 1.1969456000194248E-4 0.0 1.7099222857420354E-5 3.334348457196969E-4 84-85 0.005394804811516121 1.1969456000194248E-4 0.0 5.129766857226106E-5 3.4198445714840706E-4 86-87 0.006865337977254271 1.1969456000194248E-4 0.0 5.129766857226106E-5 3.4198445714840706E-4 88-89 0.00867785560014083 1.1969456000194248E-4 0.0 5.129766857226106E-5 3.4198445714840706E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 178650 0.0 68.58392 9 GTGGTAT 179080 0.0 68.16684 6 GGTATCA 179380 0.0 68.11883 8 TGGTATC 179675 0.0 68.00183 7 AGTGGTA 182755 0.0 66.700905 5 CAGTGGT 185490 0.0 66.32432 4 AAGCAGT 189230 0.0 65.132324 1 AGCAGTG 192415 0.0 63.951756 2 GCAGTGG 194920 0.0 63.137012 3 AGAGTAC 51020 0.0 45.459038 18-19 AGTACTT 42530 0.0 42.738014 20-21 CTTAACG 8090 0.0 42.66098 52-53 GTACTTT 41960 0.0 42.56911 22-23 GAGTACT 43570 0.0 42.41379 20-21 ACGCAGA 152575 0.0 41.880013 14-15 GCAGAGT 156700 0.0 41.699352 16-17 CAACGCA 154300 0.0 41.563828 12-13 TAACGCC 8350 0.0 41.50508 54-55 AACGCAG 153420 0.0 41.435703 14-15 TCAACGC 154910 0.0 41.130333 12-13 >>END_MODULE