FastQCFastQC Report
Sat 18 Jun 2016
SRR799682_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR799682_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8000000
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA585450.7318125No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA420060.5250750000000001No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT358190.44773749999999995No Hit
AAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAA112270.14033749999999998No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA87470.10933749999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2577800.070.818466
TGGTATC2569900.070.708117
AGTGGTA2584900.070.608585
GTATCAA2601850.070.297599
GGTATCA2606900.070.192998
CAGTGGT2598600.070.115354
AAGCAGT2709050.067.913331
AGCAGTG2770400.066.406982
GCAGTGG2796550.065.687713
GTCACCG94150.050.18058422-23
TCACCGG95100.049.89344422-23
CAGAGTA570150.045.15398418-19
CAGAGTG587400.044.82142618-19
AGAGTCA228000.044.49326318-19
GAGTACT94200.043.9919920-21
GTACTTT74350.043.50395222-23
AGAGTGG247400.043.47278618-19
AGAGTAC196450.043.33178318-19
AGAGTAG172450.042.49603718-19
GAGTCAC137250.042.4587120-21
Produced by FastQC (version 0.11.5)