##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR799682_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8000000
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.181980125	31.0	27.0	31.0	16.0	34.0
2	28.529161625	31.0	27.0	31.0	18.0	34.0
3	27.98635525	31.0	27.0	31.0	16.0	34.0
4	30.962930125	35.0	30.0	37.0	16.0	37.0
5	31.400064625	35.0	30.0	37.0	17.0	37.0
6	31.364999125	35.0	30.0	37.0	17.0	37.0
7	30.954567125	35.0	30.0	37.0	16.0	37.0
8	30.9478155	35.0	30.0	37.0	16.0	37.0
9	32.2092985	37.0	30.0	39.0	15.0	39.0
10-11	32.292085625	37.0	30.0	39.0	16.0	39.0
12-13	32.1545445625	37.0	30.0	39.0	15.0	39.0
14-15	33.4615715625	38.0	31.0	40.0	17.0	41.0
16-17	33.0158841875	38.0	30.0	40.0	16.5	41.0
18-19	33.0528444375	37.5	30.0	40.0	17.0	41.0
20-21	33.0608449375	37.0	30.0	40.0	17.0	41.0
22-23	27.6182403125	32.0	16.0	39.0	9.5	40.5
24-25	28.0881921875	31.0	20.5	38.0	12.0	40.0
26-27	30.23088775	34.0	25.0	38.0	10.0	40.0
28-29	30.95871125	35.0	26.0	39.0	11.0	40.5
30-31	30.5446178125	34.5	25.5	39.0	9.5	40.5
32-33	30.021396062500003	34.0	24.5	38.5	9.5	40.0
34-35	30.3008148125	34.0	25.0	39.0	10.0	40.0
36-37	30.365258687500003	34.0	25.0	39.0	9.5	40.0
38-39	30.26171625	34.0	25.0	38.5	10.0	40.0
40-41	30.149932	34.0	25.0	38.5	9.5	40.0
42-43	30.1633086875	33.5	25.0	38.5	10.0	40.0
44-45	29.973990875	33.0	25.0	38.0	10.0	40.0
46-47	29.721145625	33.0	24.0	38.0	9.0	40.0
48-49	29.456732125000002	33.0	24.0	38.0	9.0	40.0
50-51	29.161292625	33.0	23.5	38.0	9.0	40.0
52-53	29.0579423125	32.5	23.5	38.0	8.5	40.0
54-55	28.94489775	32.0	23.0	37.0	8.0	40.0
56-57	28.777282375	32.0	23.0	37.0	8.0	40.0
58-59	28.562075812499998	32.0	23.0	37.0	8.0	40.0
60-61	28.219556062499997	31.5	22.5	36.0	8.0	39.0
62-63	27.9997943125	31.0	22.0	36.0	8.0	39.0
64-65	27.747976625	31.0	22.0	35.0	8.0	39.0
66-67	27.545494375	31.0	22.0	35.0	8.0	38.5
68-69	27.343054625	31.0	22.0	35.0	8.0	38.0
70-71	26.926986562499998	30.0	20.5	35.0	8.0	37.5
72-73	26.7769448125	30.0	20.5	35.0	7.5	37.0
74-75	26.613756875	30.0	20.5	34.0	7.5	36.0
76-77	26.3337324375	30.0	20.0	34.0	7.0	36.0
78-79	26.1033155625	29.0	20.0	34.0	7.0	35.5
80-81	25.9413436875	29.0	20.0	34.0	7.0	35.0
82-83	25.809956125	29.0	20.0	34.0	7.0	35.0
84-85	25.634628687499998	29.0	20.0	34.0	7.0	35.0
86-87	25.508205500000003	29.0	19.5	34.0	7.0	35.0
88-89	25.2967665	29.0	19.0	34.0	7.0	35.0
90-91	25.2908885625	29.0	19.0	34.0	7.0	35.0
92-93	25.164630875	29.0	19.0	34.0	7.0	35.0
94-95	25.093568312499997	29.0	19.0	34.0	7.0	35.0
96-97	24.958639437499997	29.0	18.5	34.0	7.0	35.0
98-99	24.7885475625	28.5	18.0	33.5	7.0	35.0
100-101	23.614236	26.5	16.0	32.5	7.0	34.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.31859374465720336
1101	2	0.24861619265697144
1101	3	0.35909598938584963
1101	4	0.356154045867072
1101	5	0.19601994090800545
1101	6	0.26452698936315855
1101	7	0.2641081587399192
1101	8	0.299071365835637
1101	9	0.45622383110277553
1101	10-11	0.49044138316709507
1101	12-13	0.4395870963295252
1101	14-15	0.4807363118652006
1101	16-17	0.4461852130795876
1101	18-19	0.6174364032129844
1101	20-21	0.5667138071131674
1101	22-23	6.517600787515331
1101	24-25	5.315058671382417
1101	26-27	1.6154886416782688
1101	28-29	0.8483329319340136
1101	30-31	0.7531857158397202
1101	32-33	0.6708471981646724
1101	34-35	0.5262600411717635
1101	36-37	0.5663622260014805
1101	38-39	0.5710052893352469
1101	40-41	0.589408703331582
1101	42-43	0.539645047245628
1101	44-45	0.5001761329342962
1101	46-47	0.3560441554767948
1101	48-49	0.41623366279290863
1101	50-51	0.560699800327594
1101	52-53	0.5336878173579009
1101	54-55	0.43358613954452707
1101	56-57	0.30412569213364193
1101	58-59	0.34389579378535373
1101	60-61	0.28725803362122093
1101	62-63	0.2740810294420548
1101	64-65	0.30461328793929354
1101	66-67	0.21662040077371714
1101	68-69	0.14678910493650577
1101	70-71	0.23465822089436728
1101	72-73	0.10254517091824056
1101	74-75	0.1912313874530014
1101	76-77	0.249830617118473
1101	78-79	0.238985348778602
1101	80-81	0.19490938409429504
1101	82-83	0.07491243804693326
1101	84-85	0.04948671853926356
1101	86-87	0.022572597789022097
1101	88-89	0.14475284866233196
1101	90-91	0.0525615117808762
1101	92-93	0.009764429398543939
1101	94-95	-0.04353354313339608
1101	96-97	-0.08430796550447894
1101	98-99	-0.054296707507564435
1101	100-101	0.04717564249567374
1102	1	0.29668091683774733
1102	2	0.17056219357080948
1102	3	0.3176063593315206
1102	4	0.1911721660138106
1102	5	0.20084110114836662
1102	6	0.1748374061288871
1102	7	0.2992830102893933
1102	8	0.257398381596218
1102	9	0.34574359553487
1102	10-11	0.25872857526223925
1102	12-13	0.4219292470550897
1102	14-15	0.424858280476883
1102	16-17	0.3939294662180899
1102	18-19	0.43356769173258414
1102	20-21	0.419712564339207
1102	22-23	5.462851214953151
1102	24-25	4.69335653052638
1102	26-27	1.4757961411788543
1102	28-29	0.7005054348680773
1102	30-31	0.5863859355367751
1102	32-33	0.5268759288828981
1102	34-35	0.48631572188212857
1102	36-37	0.5354024031192743
1102	38-39	0.5118225722206908
1102	40-41	0.6214491177566437
1102	42-43	0.5694812961400615
1102	44-45	0.48720144432352797
1102	46-47	0.46392741177418984
1102	48-49	0.45899698510885045
1102	50-51	0.5084108663205775
1102	52-53	0.3989373351082257
1102	54-55	0.19924445627567522
1102	56-57	0.2158426762033585
1102	58-59	0.30671996945049074
1102	60-61	0.27962578114778225
1102	62-63	0.19092856503242217
1102	64-65	0.19585572740178492
1102	66-67	0.08732841048616535
1102	68-69	0.19544258849616725
1102	70-71	0.10809713462268888
1102	72-73	0.036944650668903734
1102	74-75	0.0787786318367445
1102	76-77	0.15267511552959334
1102	78-79	0.12548410352089334
1102	80-81	0.07499180693907448
1102	82-83	0.05858759173316841
1102	84-85	0.051519448559787406
1102	86-87	-0.02873011049513252
1102	88-89	0.10197575044096752
1102	90-91	-0.020580689756926063
1102	92-93	-0.048621497843331696
1102	94-95	0.008236887184413177
1102	96-97	-0.05468582296031599
1102	98-99	-0.11957666912447351
1102	100-101	-0.09320012208381101
1103	1	-0.12202303783247359
1103	2	-0.16936854676906776
1103	3	-0.07099005368772282
1103	4	-0.18550668142026439
1103	5	-0.3226929117189208
1103	6	-0.1431740520737783
1103	7	-0.19735305358251054
1103	8	-0.11398833655291085
1103	9	0.09895120057731788
1103	10-11	-0.20291935087865198
1103	12-13	-0.028324499299351658
1103	14-15	-0.04572763057577589
1103	16-17	-0.06666145922066846
1103	18-19	-0.013955327347233037
1103	20-21	-0.08554583128783122
1103	22-23	-9.108782889432593
1103	24-25	-7.757559140257367
1103	26-27	-1.860774172596816
1103	28-29	-0.49886799913149815
1103	30-31	-0.18013640623710359
1103	32-33	-0.071474514539144
1103	34-35	-0.06967719404482153
1103	36-37	0.0201219922934186
1103	38-39	-0.001242582496864486
1103	40-41	0.08860850993586311
1103	42-43	0.03284694743624428
1103	44-45	-0.08688126594254086
1103	46-47	-0.14194944732773607
1103	48-49	-0.1521339329356053
1103	50-51	-0.024350656233455226
1103	52-53	-0.11092954450356984
1103	54-55	-0.21232651777181744
1103	56-57	-0.23645749596489196
1103	58-59	-0.22453263845222438
1103	60-61	-0.08328320237240305
1103	62-63	-0.19687278156322918
1103	64-65	-0.2572361921244024
1103	66-67	-0.3557603352577807
1103	68-69	-0.3752447249373603
1103	70-71	-0.32088562770036333
1103	72-73	-0.35132248287021994
1103	74-75	-0.4484310707998347
1103	76-77	-0.27490619187888043
1103	78-79	-0.27655522631453877
1103	80-81	-0.3309869745876526
1103	82-83	-0.4297902354539964
1103	84-85	-0.4371286997134547
1103	86-87	-0.37423552891417344
1103	88-89	-0.30215033629272625
1103	90-91	-0.3928729283080905
1103	92-93	-0.4303135086005945
1103	94-95	-0.39889387226129713
1103	96-97	-0.4413132778255715
1103	98-99	-0.49910802024601963
1103	100-101	-0.4434460047682194
1104	1	-1.3463732368019627
1104	2	-1.2681747815126663
1104	3	-0.9882607805601005
1104	4	-1.2717407168928787
1104	5	-1.3877299008718893
1104	6	-0.5628208134567529
1104	7	-0.6051534499400759
1104	8	-0.31383958208943596
1104	9	-0.5895945718538513
1104	10-11	-0.584258656979209
1104	12-13	-0.7956558450097759
1104	14-15	-1.3153169671443763
1104	16-17	-1.6650241628918074
1104	18-19	-1.4477059127798277
1104	20-21	-1.4548800710944079
1104	22-23	-9.907526934498584
1104	24-25	-8.254762961947122
1104	26-27	-3.4907167342265524
1104	28-29	-2.133309476814823
1104	30-31	-1.8757866231382891
1104	32-33	-1.9746707437269428
1104	34-35	-1.780181432941042
1104	36-37	-1.9017082763492041
1104	38-39	-1.851374868058901
1104	40-41	-1.870723255685153
1104	42-43	-1.897779816409141
1104	44-45	-1.7875355556013908
1104	46-47	-1.750661881558539
1104	48-49	-1.954712470694389
1104	50-51	-2.1437202344776765
1104	52-53	-1.95601978234955
1104	54-55	-2.019842194383248
1104	56-57	-1.9265467982025193
1104	58-59	-1.876350240755933
1104	60-61	-1.814488804910706
1104	62-63	-1.721141071726727
1104	64-65	-1.8060076736154898
1104	66-67	-1.7526798348185935
1104	68-69	-1.911206210266478
1104	70-71	-1.937967172339338
1104	72-73	-1.9848443575518644
1104	74-75	-1.9205286668757502
1104	76-77	-1.7742928681992218
1104	78-79	-1.7439773945024264
1104	80-81	-1.9473176988509238
1104	82-83	-1.999579932835534
1104	84-85	-2.0256288696469937
1104	86-87	-1.9323353647392096
1104	88-89	-1.9322118626374838
1104	90-91	-1.8197324772961743
1104	92-93	-1.8774095228479482
1104	94-95	-1.97886109337108
1104	96-97	-2.0939741574072137
1104	98-99	-2.0248583706113585
1104	100-101	-1.9232922187192258
1105	1	0.40499448715046427
1105	2	0.4097018898802105
1105	3	0.3142257893104201
1105	4	0.4191941206355736
1105	5	0.4867156830406394
1105	6	0.4992543482386509
1105	7	0.02799829689600486
1105	8	0.3938544998758893
1105	9	0.26470957895014635
1105	10-11	0.1469093524597156
1105	12-13	0.40163325568383357
1105	14-15	0.6754639075028379
1105	16-17	0.32243851787524136
1105	18-19	0.29361921420493076
1105	20-21	0.6500176179073591
1105	22-23	3.904170499410931
1105	24-25	3.9960975299811956
1105	26-27	1.1639895825840298
1105	28-29	0.5534958416351898
1105	30-31	0.2991247179884162
1105	32-33	-0.08088183574707486
1105	34-35	0.16987227337850186
1105	36-37	0.3498469293428741
1105	38-39	0.23963246967015195
1105	40-41	0.30972500368715217
1105	42-43	0.37068309910759467
1105	44-45	0.21652084671694638
1105	46-47	0.17185875254353533
1105	48-49	0.09889646356783288
1105	50-51	0.1720954959604093
1105	52-53	0.22083652683523525
1105	54-55	0.26891408656136306
1105	56-57	0.3484695126650692
1105	58-59	0.5523847976717207
1105	60-61	0.5029740924301613
1105	62-63	0.45795096583427153
1105	64-65	0.23059954494836177
1105	66-67	0.21966476814290914
1105	68-69	0.17823486211293016
1105	70-71	0.19778253644084032
1105	72-73	0.25209480925510874
1105	74-75	0.25001838604229576
1105	76-77	0.2439696484618672
1105	78-79	0.3832637397099212
1105	80-81	0.35807438334450836
1105	82-83	0.27364265668693477
1105	84-85	0.28548453459865897
1105	86-87	0.07554162533907416
1105	88-89	0.2147828022070435
1105	90-91	0.08640704130759147
1105	92-93	0.17849092675521305
1105	94-95	0.030361187716042792
1105	96-97	0.10556919275984811
1105	98-99	0.024304897368320866
1105	100-101	0.010817959160036139
1106	1	-0.29575170290458175
1106	2	-0.3754084327176912
1106	3	-0.7064840221571309
1106	4	-0.4966731065311265
1106	5	-0.42818371993419646
1106	6	-0.24931500575197418
1106	7	-0.2992756906011067
1106	8	-0.3540658454533947
1106	9	-0.33653857110893526
1106	10-11	-0.2723765098539985
1106	12-13	-0.7894691059009453
1106	14-15	-0.8735599118371269
1106	16-17	-0.4939559806636211
1106	18-19	-1.580865086580168
1106	20-21	-1.5182883561656375
1106	22-23	4.12372098043539
1106	24-25	3.7885729752614097
1106	26-27	0.54625168678443
1106	28-29	-1.1088198941458032
1106	30-31	-0.47296573114265783
1106	32-33	-0.30315870837606695
1106	34-35	-0.3877196830039651
1106	36-37	-0.2484005910191236
1106	38-39	-0.765596804373569
1106	40-41	-1.5332655912698705
1106	42-43	-0.777559440140994
1106	44-45	-1.028211532933792
1106	46-47	-0.5996256194046587
1106	48-49	-0.44767116700457876
1106	50-51	-1.9062328120385743
1106	52-53	-1.2507312140999005
1106	54-55	-0.6842466388665436
1106	56-57	-1.1225963168104585
1106	58-59	-1.0214045835593097
1106	60-61	-0.600334594138598
1106	62-63	-0.42683834611475646
1106	64-65	-0.3206687715279912
1106	66-67	-0.33102331347338065
1106	68-69	-0.35181196945521265
1106	70-71	-1.0888796377424192
1106	72-73	-0.7657749787211863
1106	74-75	-0.7019862310789229
1106	76-77	-0.9230694677431366
1106	78-79	-0.30111892011726127
1106	80-81	-0.5470820164107835
1106	82-83	-0.4160086967764691
1106	84-85	-0.485855660398542
1106	86-87	-0.4908097179618558
1106	88-89	-1.0452271764035217
1106	90-91	-0.5352598356929832
1106	92-93	-0.4986386039019379
1106	94-95	-0.4621451833987962
1106	96-97	-0.37827642764605685
1106	98-99	-0.4000544796410104
1106	100-101	-0.36180377536059183
1107	1	0.15400319783282868
1107	2	0.1394621477463005
1107	3	0.24764334652109454
1107	4	0.14981449355461152
1107	5	0.241374386892943
1107	6	-0.0015587330410689049
1107	7	-0.31521068162182075
1107	8	-0.34832198498240174
1107	9	-0.18420614062172547
1107	10-11	0.179886939560852
1107	12-13	-0.3913023253304644
1107	14-15	0.19017150966429597
1107	16-17	0.2362260663836082
1107	18-19	0.27713543069877744
1107	20-21	-0.16642498813333617
1107	22-23	4.519835520371039
1107	24-25	4.205136278601934
1107	26-27	0.9083347206432819
1107	28-29	0.03568945464312279
1107	30-31	0.17020948026291194
1107	32-33	-0.1363766023042956
1107	34-35	-0.07193292377281324
1107	36-37	-0.23194935750991164
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2109	18-19	-0.23421550194512264
2109	20-21	-0.14938401097519005
2109	22-23	-9.30016417650473
2109	24-25	-7.7216200918723885
2109	26-27	-1.8129445811044498
2109	28-29	-0.4923521423340773
2109	30-31	-0.26708603672708975
2109	32-33	-0.39212052534266917
2109	34-35	-0.4827819910964095
2109	36-37	-0.3333402230393574
2109	38-39	-0.22762732283737108
2109	40-41	0.03365412249147326
2109	42-43	-0.1457312264900068
2109	44-45	-0.06764009873760912
2109	46-47	-0.11033402417554683
2109	48-49	-0.051207785612657375
2109	50-51	0.13147497992352797
2109	52-53	0.08833581704857707
2109	54-55	0.006556213516041964
2109	56-57	0.10597500671408255
2109	58-59	0.10746278951969757
2109	60-61	0.13410402399221866
2109	62-63	0.009256625047690648
2109	64-65	-0.006278640587403572
2109	66-67	0.051621940524071874
2109	68-69	0.10300148904704542
2109	70-71	0.23679750522964937
2109	72-73	0.1779180374745195
2109	74-75	0.24002421073469193
2109	76-77	0.2857783128193354
2109	78-79	0.1833101750753876
2109	80-81	0.10711000105114721
2109	82-83	0.013003612628352812
2109	84-85	0.13119554913800968
2109	86-87	0.23818024060597764
2109	88-89	0.26816228751700066
2109	90-91	0.14402499331194107
2109	92-93	0.2320180011968418
2109	94-95	0.3073284383436139
2109	96-97	0.27972548434597755
2109	98-99	0.3377633786101981
2109	100-101	0.3104005113406032
2110	1	0.5003681405680389
2110	2	0.3844185003668734
2110	3	0.6307145119608357
2110	4	0.48160201450990314
2110	5	0.5450382967357399
2110	6	0.48127453083454697
2110	7	0.48937586570183456
2110	8	0.5085836982711669
2110	9	-0.08768577054039639
2110	10-11	0.057115912073761876
2110	12-13	0.36844396975616434
2110	14-15	0.5356906665951442
2110	16-17	0.10637346328917374
2110	18-19	0.5432492267651412
2110	20-21	0.31725036456554534
2110	22-23	-8.869920474607515
2110	24-25	-7.355030884228526
2110	26-27	-1.6535909934183053
2110	28-29	-0.296106539252186
2110	30-31	-0.21618520716338452
2110	32-33	0.23397989575774147
2110	34-35	0.286596926990093
2110	36-37	0.5678462603794152
2110	38-39	0.6471847466189153
2110	40-41	0.7328902441242526
2110	42-43	0.6986624166356101
2110	44-45	0.7389203930563149
2110	46-47	0.7648707288623342
2110	48-49	0.772030821159138
2110	50-51	0.8397467998126622
2110	52-53	0.7355032670984372
2110	54-55	0.8204468464484584
2110	56-57	0.7818659965709713
2110	58-59	-1.100040247623781
2110	60-61	-0.3984639152193914
2110	62-63	0.44798428738952367
2110	64-65	0.7385755427188379
2110	66-67	0.7160463840985329
2110	68-69	0.632282532881618
2110	70-71	0.6710789580798604
2110	72-73	0.606898295303882
2110	74-75	0.6921475732793922
2110	76-77	-1.0433873114685142
2110	78-79	-0.5991499535183671
2110	80-81	0.30140900395768355
2110	82-83	0.5804751846130571
2110	84-85	0.644427528808933
2110	86-87	0.6444956801320849
2110	88-89	0.743916721384597
2110	90-91	0.7078326470182574
2110	92-93	0.6695998721762635
2110	94-95	0.7175754323643702
2110	96-97	0.8222925274371775
2110	98-99	0.7672950980824211
2110	100-101	0.8417131106126021
2111	1	0.1965090018242961
2111	2	0.22215867648129972
2111	3	0.3947489406742015
2111	4	0.1698997517078702
2111	5	0.3369427874265085
2111	6	0.23685001547732654
2111	7	0.06732740605108845
2111	8	-0.09892955826413896
2111	9	0.11545785601692415
2111	10-11	0.27218768623271217
2111	12-13	0.10590378792100807
2111	14-15	0.19304075606572013
2111	16-17	0.12669443002563696
2111	18-19	0.15385281450560484
2111	20-21	0.2717313805676085
2111	22-23	-9.142443544722664
2111	24-25	-7.876793349325752
2111	26-27	-2.3259224189995678
2111	28-29	-0.3923768320779679
2111	30-31	-1.592647506275238
2111	32-33	-0.5484458041275921
2111	34-35	-0.055392238952368444
2111	36-37	-0.08302917992768499
2111	38-39	0.18423872930023677
2111	40-41	-0.10670870729915904
2111	42-43	-0.35463731019941847
2111	44-45	-0.5172614471518493
2111	46-47	0.08033735974638034
2111	48-49	0.4744783271301536
2111	50-51	-0.03196232786819664
2111	52-53	0.45672436583238607
2111	54-55	0.5885745012258177
2111	56-57	0.6725853702517313
2111	58-59	-0.724874072968138
2111	60-61	-0.4703863084372948
2111	62-63	0.2586444094262035
2111	64-65	0.5610592407348349
2111	66-67	0.587577874626934
2111	68-69	0.5462194146954928
2111	70-71	0.6133387651397477
2111	72-73	0.5871168441055161
2111	74-75	0.6276670115707788
2111	76-77	-0.872713720472273
2111	78-79	-0.678163068297291
2111	80-81	0.314536612464412
2111	82-83	0.5061733032492661
2111	84-85	0.5857781180023025
2111	86-87	0.6735143473865328
2111	88-89	0.7830098086367059
2111	90-91	0.7031465679997986
2111	92-93	0.7216417950490097
2111	94-95	0.685559227548687
2111	96-97	0.7913689404370352
2111	98-99	0.8429410991643067
2111	100-101	0.6306641890968407
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	190.0
3	296.0
4	275.0
5	271.0
6	333.0
7	729.0
8	3322.0
9	11757.0
10	36418.0
11	81296.0
12	123713.0
13	145311.0
14	151906.0
15	155802.0
16	158901.0
17	163904.0
18	168523.0
19	175587.0
20	184561.0
21	195555.0
22	208486.0
23	225719.0
24	244339.0
25	253301.0
26	254200.0
27	257949.0
28	276969.0
29	298013.0
30	325450.0
31	366373.0
32	420784.0
33	487887.0
34	582052.0
35	671216.0
36	659383.0
37	501103.0
38	191676.0
39	16446.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.310559148453388	47.94672552681362	21.34504179056251	17.397673534170472
2	17.277552369669074	49.40616368512835	16.192776193836416	17.12350775136616
3	43.18412235974143	22.94980306481976	16.96945744961096	16.896617125827845
4	17.091541439825477	22.924370264842484	16.727520247225332	43.256568048106715
5	17.309961758491752	48.8094647201551	17.67713624901652	16.203437272336625
6	42.77288462567386	24.122720388109688	16.397479407174647	16.706915579041798
7	17.34003542931791	23.37109659840538	42.95988595000485	16.328982022271855
8	42.00247257730742	24.459025263469254	16.876521529537474	16.661980629685853
9	41.831250901031396	24.2909187663225	17.254127677196383	16.62370265544972
10-11	15.948302806385506	36.95419932648348	30.471177763753126	16.626320103377886
12-13	15.746146525760876	24.472361265581544	30.07583764581163	29.70565456284595
14-15	16.071935372638293	49.651477943064734	17.474494101655516	16.80209258264146
16-17	29.129572860643066	23.48116844285594	17.456009105931596	29.9332495905694
18-19	16.392167535900022	36.03058629588823	17.69518619704854	29.882059971163216
20-21	29.55138257859523	35.863340994757515	17.786224075498875	16.79905235114838
22-23	31.976011627035696	21.840949298659567	28.03526726891575	18.14777180538899
24-25	24.624868954187978	29.748607779923702	21.975975429516033	23.650547836372283
26-27	26.618406837983017	26.542416050654687	22.243513118804454	24.595663992557842
28-29	42.35083408600268	21.786584340780752	18.793891427934454	17.068690145282115
30-31	34.90861149645017	25.920268451072026	20.671806660409544	18.49931339206826
32-33	24.922103441528034	29.641430128171862	24.322207099374616	21.114259330925485
34-35	23.209967233940663	28.089490541230784	23.782319831999704	24.918222392828852
36-37	25.86081266685018	26.463978741706217	25.225637115188416	22.44957147625519
38-39	25.98794407246061	26.12203706811326	24.251531374897432	23.638487484528692
40-41	22.526580346161225	28.72052465398354	25.54869849836138	23.20419650149386
42-43	23.050525079064837	27.202393600622738	25.76680728891061	23.980274031401812
44-45	22.861887439364857	29.73516255495744	23.32110000415198	24.081850001525726
46-47	24.857653632542707	26.161806088233703	23.823587629407566	25.156952649816024
48-49	23.754361547497783	27.72218383232795	23.83961775798306	24.683836862191203
50-51	24.470882379907007	28.982320781571474	23.509615607113915	23.0371812314076
52-53	25.13802835994377	25.886503824913902	25.35034745711365	23.625120358028674
54-55	24.51150517495318	27.352778270947997	24.755786970470634	23.379929583628194
56-57	24.82546136407041	27.146070829437907	23.52890024983879	24.49956755665289
58-59	27.277436317263216	25.949156096590514	23.767169014389193	23.00623857175708
60-61	24.962437343989144	26.750282212314445	23.762889634007486	24.52439080968892
62-63	25.20463505976474	27.69028884520695	23.82101917088972	23.284056924138582
64-65	23.565940899814514	26.516429775037302	25.847773340811898	24.069855984336286
66-67	25.085453750443413	27.113536921884453	25.057582892669938	22.743426435002196
68-69	26.94197851936133	26.565067080108527	23.91312719588892	22.579827204641216
70-71	23.160368362335856	27.271729951634015	25.88043520319332	23.687466482836804
72-73	24.045977784664117	26.306758078941893	24.776549149309833	24.87071498708416
74-75	24.345369410244047	28.331376301023266	24.47139341388142	22.85186087485127
76-77	24.934454521536292	25.579629684846	25.023992554916276	24.461923238701434
78-79	23.899443292994917	26.646314609628398	23.991393249064107	25.462848848312582
80-81	25.054240539914684	26.788606185672194	24.864842527384095	23.292310747029024
82-83	25.09865360483332	26.38369014587974	25.254453876661724	23.263202372625212
84-85	26.842843742393253	26.28406243698161	23.648238698071438	23.2248551225537
86-87	24.506195120896095	26.54422291957802	24.325190741007702	24.624391218518184
88-89	26.29457493445283	25.72205350014282	23.616860821570352	24.366510743833995
90-91	24.968148343270034	26.800417162626538	23.516355392853352	24.715079101250076
92-93	24.351037814401128	27.93475434266754	25.038081949955863	22.676125892975467
94-95	23.47113843599751	27.17140546203255	25.5384775015564	23.81897860041354
96-97	25.270710979280427	26.05557309362097	24.565439036816393	24.10827689028221
98-99	26.615584044542096	26.170075561144575	24.17415854492986	23.040181849383472
100-101	23.87225788244603	28.21059392793751	24.630895271016904	23.286252918599555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70490.0
1	47571.5
2	18286.5
3	10395.0
4	8105.0
5	7013.5
6	6441.5
7	6203.0
8	6215.0
9	6213.5
10	6295.5
11	7136.5
12	23767.0
13	25107.0
14	9678.0
15	8611.5
16	8770.0
17	9473.5
18	10467.0
19	11554.0
20	12882.5
21	14928.5
22	17621.5
23	20704.0
24	24054.0
25	27826.0
26	32521.5
27	37900.5
28	43204.0
29	48978.5
30	55852.5
31	62921.5
32	70341.0
33	78693.5
34	87968.5
35	98043.5
36	107976.0
37	117022.5
38	124910.0
39	132861.5
40	143126.5
41	156536.0
42	170311.0
43	182856.0
44	196641.0
45	211115.0
46	226893.0
47	242100.5
48	255012.0
49	267723.5
50	281175.5
51	301426.5
52	333264.5
53	383711.5
54	393821.5
55	364497.5
56	349702.5
57	327936.0
58	293664.0
59	257609.5
60	222676.0
61	186557.5
62	153148.0
63	124415.5
64	101101.0
65	81910.5
66	66298.5
67	53852.5
68	42479.5
69	32513.0
70	24854.5
71	19304.0
72	15258.5
73	12037.0
74	9170.5
75	6943.0
76	5212.0
77	3791.0
78	2836.5
79	2087.0
80	1412.0
81	971.5
82	687.5
83	508.0
84	369.5
85	247.5
86	148.0
87	106.0
88	75.5
89	43.5
90	30.5
91	22.5
92	14.0
93	6.5
94	3.5
95	3.0
96	3.0
97	4.0
98	4.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.1135125
2	0.02085
3	0.0210375
4	0.026425
5	0.0202875
6	0.0076500000000000005
7	0.0263
8	0.013237500000000001
9	0.0277625
10-11	0.0173
12-13	0.02244375
14-15	0.020675000000000002
16-17	0.0212125
18-19	0.014862499999999999
20-21	0.01043125
22-23	15.838393749999998
24-25	0.05284375
26-27	0.029
28-29	0.02941875
30-31	0.033925
32-33	0.0316875
34-35	0.0299375
36-37	0.02960625
38-39	0.036725
40-41	0.05126875
42-43	0.054337500000000004
44-45	0.04765
46-47	0.06440625
48-49	0.06593125
50-51	0.06416250000000001
52-53	0.044318750000000004
54-55	0.02325
56-57	0.02303125
58-59	0.0352125
60-61	0.0420125
62-63	0.047675
64-65	0.03648125
66-67	0.0299875
68-69	0.02625
70-71	0.01624375
72-73	0.023456249999999998
74-75	0.02400625
76-77	0.01455625
78-79	0.02034375
80-81	0.028650000000000002
82-83	0.02424375
84-85	0.0167875
86-87	0.0231375
88-89	0.026668749999999998
90-91	0.0150125
92-93	0.01825
94-95	0.00975
96-97	0.0190625
98-99	0.02715625
100-101	0.02964375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8000000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.44282757102185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.73231619533287	56.76008013606516
2	7.129990129651779	9.332134292757784
3	2.0818224518180117	4.087210432434243
4	1.041166054485199	2.7254740230590415
5	0.6467274252168859	2.11618356869598
6	0.47018481097164855	1.8462134106558665
7	0.3549897999228552	1.6262075386076071
8	0.26834374595279087	1.404893879692047
9	0.2156640088206636	1.2702296288273425
>10	0.9858737102067442	10.582080639079287
>50	0.04879424838983319	2.1691320353353176
>100	0.021666382134869475	2.602164708285304
>500	0.0015735480803810307	0.7142954091606067
>1k	7.866379974223849E-4	0.699967071362242
>5k	2.0170203588933647E-5	0.1154599762553454
>10k+	8.068081435573459E-5	1.9482732497267883
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58545	0.7318125	No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA	42006	0.5250750000000001	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35819	0.44773749999999995	No Hit
AAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAA	11227	0.14033749999999998	No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA	8747	0.10933749999999999	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	1.25E-5	0.0	0.0	0.0	0.0
4	1.25E-5	0.0	0.0	0.0	1.25E-5
5	1.25E-5	0.0	0.0	0.0	3.75E-5
6	1.25E-5	0.0	0.0	0.0	3.75E-5
7	2.5E-5	1.25E-5	0.0	0.0	3.75E-5
8	2.5E-5	1.25E-5	0.0	0.0	3.75E-5
9	2.5E-5	1.25E-5	0.0	0.0	3.75E-5
10-11	2.5E-5	1.25E-5	0.0	0.0	3.75E-5
12-13	2.5E-5	1.25E-5	0.0	0.0	5.0E-5
14-15	5.0E-5	1.25E-5	0.0	0.0	5.0E-5
16-17	5.0E-5	2.5E-5	0.0	0.0	5.0E-5
18-19	6.25E-5	3.75E-5	0.0	0.0	5.0E-5
20-21	8.125E-5	3.75E-5	0.0	0.0	5.0E-5
22-23	1.0E-4	4.375E-5	0.0	0.0	6.25E-5
24-25	1.1875000000000001E-4	5.0E-5	0.0	1.25E-5	6.25E-5
26-27	1.625E-4	5.0E-5	0.0	1.25E-5	6.25E-5
28-29	2.0E-4	5.0E-5	0.0	1.25E-5	6.25E-5
30-31	2.1875E-4	5.0E-5	0.0	1.25E-5	6.25E-5
32-33	2.9375E-4	5.0E-5	0.0	2.5E-5	8.125E-5
34-35	3.5625E-4	5.0E-5	0.0	2.5E-5	9.375E-5
36-37	4.125E-4	5.0E-5	0.0	2.5E-5	1.0E-4
38-39	4.5E-4	5.0E-5	0.0	2.5E-5	1.375E-4
40-41	5.375E-4	5.0E-5	0.0	2.5E-5	1.375E-4
42-43	5.625E-4	6.25E-5	0.0	2.5E-5	1.375E-4
44-45	7.0E-4	6.25E-5	0.0	3.75E-5	1.375E-4
46-47	8.0E-4	6.25E-5	0.0	4.375E-5	1.375E-4
48-49	0.00100625	6.25E-5	0.0	5.0E-5	1.375E-4
50-51	0.00115625	6.25E-5	0.0	5.0E-5	1.375E-4
52-53	0.0012875	6.874999999999999E-5	0.0	5.0E-5	1.375E-4
54-55	0.0013874999999999998	7.5E-5	0.0	5.0E-5	1.375E-4
56-57	0.00145	7.5E-5	0.0	5.625E-5	1.5E-4
58-59	0.00154375	7.5E-5	0.0	6.25E-5	1.5E-4
60-61	0.0016250000000000001	7.5E-5	0.0	6.25E-5	1.5624999999999998E-4
62-63	0.00179375	7.5E-5	0.0	6.25E-5	1.625E-4
64-65	0.0019437500000000002	8.125E-5	0.0	8.75E-5	1.75E-4
66-67	0.00211875	9.375E-5	0.0	8.75E-5	1.875E-4
68-69	0.00233125	1.0E-4	0.0	8.75E-5	1.875E-4
70-71	0.0025625	1.0E-4	0.0	1.0E-4	1.875E-4
72-73	0.00283125	1.0E-4	0.0	1.0E-4	1.875E-4
74-75	0.0031125	1.0E-4	0.0	1.0E-4	1.875E-4
76-77	0.0033812499999999997	1.1875E-4	0.0	1.0E-4	1.875E-4
78-79	0.00384375	1.3125000000000002E-4	0.0	1.0E-4	1.875E-4
80-81	0.00451875	1.375E-4	0.0	1.0E-4	1.875E-4
82-83	0.0052125	1.375E-4	0.0	1.0E-4	2.0E-4
84-85	0.006575	1.375E-4	0.0	1.0625000000000001E-4	2.0E-4
86-87	0.00821875	1.375E-4	0.0	1.125E-4	2.0E-4
88-89	0.01005	1.375E-4	0.0	1.125E-4	2.0E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGGTAT	257780	0.0	70.81846	6
TGGTATC	256990	0.0	70.70811	7
AGTGGTA	258490	0.0	70.60858	5
GTATCAA	260185	0.0	70.29759	9
GGTATCA	260690	0.0	70.19299	8
CAGTGGT	259860	0.0	70.11535	4
AAGCAGT	270905	0.0	67.91333	1
AGCAGTG	277040	0.0	66.40698	2
GCAGTGG	279655	0.0	65.68771	3
GTCACCG	9415	0.0	50.180584	22-23
TCACCGG	9510	0.0	49.893444	22-23
CAGAGTA	57015	0.0	45.153984	18-19
CAGAGTG	58740	0.0	44.821426	18-19
AGAGTCA	22800	0.0	44.493263	18-19
GAGTACT	9420	0.0	43.99199	20-21
GTACTTT	7435	0.0	43.503952	22-23
AGAGTGG	24740	0.0	43.472786	18-19
AGAGTAC	19645	0.0	43.331783	18-19
AGAGTAG	17245	0.0	42.496037	18-19
GAGTCAC	13725	0.0	42.45871	20-21
>>END_MODULE