FastQCFastQC Report
Sat 18 Jun 2016
SRR799682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR799682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8000000
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4332495.4156125No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATACCCTGCA471620.5895250000000001No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440150.5501874999999999No Hit
AAGCAGTGGTATCAACGCAGAGTCACCGGGTTTCGGGTCTATATCCTGCA103500.129375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2564950.069.8687069
GTGGTAT2578750.069.519646
GGTATCA2581050.069.468
TGGTATC2581400.069.434327
AGTGGTA2626600.068.199055
CAGTGGT2650850.067.585254
AAGCAGT2690150.066.431181
AGCAGTG2750350.065.082462
GCAGTGG2781300.064.341433
AGAGTAC758050.044.6174618-19
GTACTTT629550.043.58870722-23
AGTACTT641450.043.35433220-21
GAGTACT654700.043.18623720-21
CTTAACG117400.042.641952-53
TACTTTT667050.041.3551622-23
TAACGCC122900.040.86853854-55
CTATACC99300.040.3286938-39
ACTTTTT686050.040.12139524-25
TTAACGC125800.039.9827452-53
TATACCC100850.039.8446840-41
Produced by FastQC (version 0.11.5)