FastQCFastQC Report
Sat 18 Jun 2016
SRR578610_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578610_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000641
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT151222515.12128072590547No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT316560.3165397098046015No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA234500.23448496951345418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCCGCAGCGGCTTCTGA108160.1081530673883804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3167050.091.998111
AGTGGTA3174250.091.3235865
AGCAGTG3198300.090.998292
GGTATCA3189200.090.942978
GTATCAA3189000.090.900929
TGGTATC3188700.090.869597
GTGGTAT3197450.090.655766
GCAGTGG3209100.090.485833
CAGTGGT3212000.090.050114
ATCAACG3194650.045.92513310-11
CAACGCA3194200.045.90934412-13
TCAACGC3191050.045.8400112-13
ACGCAGA3210500.045.7377214-15
AACGCAG3208400.045.66695814-15
TATCAAC3203950.045.571610-11
CGCAGAG3216400.045.54125216-17
AGAGTAC3146900.045.4587818-19
GAGTACT1832050.045.33088720-21
CAGAGTA3192800.045.31552518-19
GCAGAGT3227700.045.22654316-17
Produced by FastQC (version 0.11.5)