##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578610_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10000641 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.686722781069733 31.0 31.0 34.0 27.0 34.0 2 30.985627821256656 31.0 31.0 34.0 27.0 34.0 3 31.086247971505028 31.0 31.0 34.0 27.0 34.0 4 34.49084793664726 37.0 35.0 37.0 30.0 37.0 5 34.57906218211413 37.0 35.0 37.0 30.0 37.0 6 34.573111763535955 37.0 35.0 37.0 30.0 37.0 7 34.522553704307555 37.0 35.0 37.0 31.0 37.0 8 34.50306015384414 37.0 35.0 37.0 30.0 37.0 9 35.91357883959638 39.0 35.0 39.0 30.0 39.0 10-11 35.85478010859504 39.0 35.0 39.0 30.0 39.0 12-13 35.93417431942612 39.0 35.0 39.0 30.0 39.0 14-15 37.0214646241176 40.0 37.0 41.0 31.0 41.0 16-17 36.97647880770843 40.0 36.5 41.0 31.0 41.0 18-19 36.94956898262821 40.0 36.5 41.0 30.5 41.0 20-21 36.80092971040556 39.5 36.0 41.0 30.0 41.0 22-23 36.47270919934032 39.5 36.0 40.5 29.5 41.0 24-25 36.40568564555012 39.0 36.0 40.5 30.0 41.0 26-27 36.31385108214563 39.0 36.0 41.0 29.0 41.0 28-29 36.065250017473886 39.0 35.5 41.0 27.5 41.0 30-31 35.82248577866159 39.0 35.0 41.0 26.5 41.0 32-33 35.40765186951516 38.0 34.0 40.0 25.5 41.0 34-35 35.18853576485748 38.0 34.0 40.0 25.0 41.0 36-37 34.826058449653374 38.0 34.0 39.5 25.0 41.0 38-39 34.01181479267179 36.0 33.0 39.5 24.0 41.0 40-41 34.17143801082351 37.0 33.0 40.0 24.0 41.0 42-43 34.09290659468728 37.0 33.0 40.0 24.0 41.0 44-45 33.964749609550026 36.5 33.0 40.0 23.0 41.0 46-47 33.78059541383398 36.0 33.0 40.0 23.0 41.0 48-49 33.46727694754766 36.0 33.0 40.0 22.0 41.0 50-51 32.27110437220974 35.5 31.5 39.0 17.5 40.5 52-53 31.873707745333526 35.0 31.0 39.0 11.0 40.0 54-55 31.31501375761814 36.0 30.5 39.5 2.0 41.0 56-57 30.727103192685348 35.5 29.0 40.0 2.0 41.0 58-59 30.434446801959993 35.0 29.0 39.0 2.0 41.0 60-61 30.173165300104262 35.0 28.5 39.0 2.0 41.0 62-63 29.85069992013512 35.0 28.0 39.0 2.0 41.0 64-65 29.516864618977923 35.0 28.0 38.5 2.0 40.0 66-67 29.20032210935279 35.0 28.0 38.0 2.0 40.0 68-69 28.874589438816972 35.0 26.5 37.0 2.0 40.0 70-71 28.506691871051068 34.5 26.0 37.0 2.0 39.0 72-73 28.12141261745122 34.0 26.0 36.0 2.0 39.0 74-75 27.759454218984565 34.0 26.0 36.0 2.0 39.0 76-77 27.385018370322463 34.0 25.0 35.0 2.0 37.5 78-79 27.04116121156634 34.0 24.5 35.0 2.0 37.0 80-81 26.69787781603199 34.0 24.0 35.0 2.0 37.0 82-83 26.34329644469789 33.5 23.0 35.0 2.0 36.0 84-85 26.015097332261004 33.0 21.0 35.0 2.0 36.0 86-87 25.700949469139026 33.0 20.0 35.0 2.0 35.5 88-89 25.350219000962035 33.0 18.5 35.0 2.0 35.0 90-91 24.990414564426423 33.0 16.5 35.0 2.0 35.0 92-93 24.636401006695472 32.0 8.5 35.0 2.0 35.0 94-95 24.212692866387265 32.0 2.0 35.0 2.0 35.0 96-97 23.724533807382947 32.0 2.0 35.0 2.0 35.0 98-99 23.191743459244265 32.0 2.0 35.0 2.0 35.0 100-101 21.888864523784026 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.550145586209819 1101 2 0.5980198415501512 1101 3 0.5485113544561635 1101 4 0.6448480616533061 1101 5 0.6254200202221227 1101 6 0.47979015644403944 1101 7 0.42547642843161526 1101 8 0.4975393949476441 1101 9 0.6402782143909818 1101 10-11 0.6770648507725809 1101 12-13 0.7223698632304476 1101 14-15 0.7742006149651672 1101 16-17 0.7836646346242944 1101 18-19 0.7628411209687371 1101 20-21 0.8163413900363921 1101 22-23 0.8132891211734972 1101 24-25 0.854042700900898 1101 26-27 0.9919552889664516 1101 28-29 0.9672484395570251 1101 30-31 1.0201739118644397 1101 32-33 1.0480094250739 1101 34-35 1.1730444864211549 1101 36-37 1.1478511017210735 1101 38-39 1.0777014614093474 1101 40-41 1.1256770799843068 1101 42-43 1.390371315810306 1101 44-45 1.3014613462678213 1101 46-47 1.384899610800673 1101 48-49 1.345764390791814 1101 50-51 1.297537121518431 1101 52-53 1.297283248124426 1101 54-55 1.141847745867782 1101 56-57 1.1312563938193314 1101 58-59 1.0635860286208754 1101 60-61 1.134707343987646 1101 62-63 1.1350055539067725 1101 64-65 1.0291743751786875 1101 66-67 1.0907657432817786 1101 68-69 1.1206778740627925 1101 70-71 1.2010328800004757 1101 72-73 1.309913584005301 1101 74-75 1.2303019443044967 1101 76-77 1.2404076917086506 1101 78-79 1.1763506258830354 1101 80-81 1.206443994555631 1101 82-83 1.218173742994889 1101 84-85 1.182781845405632 1101 86-87 1.132553091118428 1101 88-89 1.255955391883699 1101 90-91 1.2555885947241947 1101 92-93 1.3629162991937314 1101 94-95 1.4191527650847817 1101 96-97 1.4992202440414673 1101 98-99 1.6874941670371264 1101 100-101 1.953966108625238 1102 1 0.519165728729245 1102 2 0.4744971744669435 1102 3 0.4795168900299842 1102 4 0.586845907693494 1102 5 0.5072773968517694 1102 6 0.4247160166589552 1102 7 0.4086254357480641 1102 8 0.5485650787620955 1102 9 0.6065016130330179 1102 10-11 0.7279391102797845 1102 12-13 0.6307914605020812 1102 14-15 0.6783026098323361 1102 16-17 0.720201104560033 1102 18-19 0.6446025256647658 1102 20-21 0.796830188238637 1102 22-23 0.7120206445209689 1102 24-25 0.6738441310182921 1102 26-27 0.8731934346033086 1102 28-29 0.806699226440827 1102 30-31 0.9914994865770481 1102 32-33 1.0171403761859494 1102 34-35 0.9121913717972916 1102 36-37 0.9426938819340478 1102 38-39 1.0184596050692534 1102 40-41 1.069660951776335 1102 42-43 1.1255062983974184 1102 44-45 1.1223505736851038 1102 46-47 1.280953386429914 1102 48-49 1.09817097044143 1102 50-51 1.1819765047037976 1102 52-53 1.1013070740872415 1102 54-55 0.973306184159906 1102 56-57 1.1051520855679833 1102 58-59 0.8962237313022072 1102 60-61 1.1487124529714663 1102 62-63 1.1776125395116068 1102 64-65 1.062846605496329 1102 66-67 1.1669114004267804 1102 68-69 1.1649742003165926 1102 70-71 1.1031863446127623 1102 72-73 1.1765116876148909 1102 74-75 1.1870593573815533 1102 76-77 1.1279743887649865 1102 78-79 0.9960401945146273 1102 80-81 1.0395896773258464 1102 82-83 1.026561258258834 1102 84-85 0.9926332571791221 1102 86-87 1.0242267638516331 1102 88-89 1.156737504007591 1102 90-91 1.287658398560584 1102 92-93 1.4411138095485008 1102 94-95 1.4331087198230676 1102 96-97 1.5554013976287209 1102 98-99 1.6102978859711925 1102 100-101 1.9342701264799942 1103 1 0.41023042028324497 1103 2 0.522998696199025 1103 3 0.4421143896850097 1103 4 0.5157241154430494 1103 5 0.41003328319005306 1103 6 0.3352496544111361 1103 7 0.34031852409660246 1103 8 0.42648424518127115 1103 9 0.4529403317633154 1103 10-11 0.5369156912415178 1103 12-13 0.48823850596097174 1103 14-15 0.608981717123406 1103 16-17 0.5341089051900809 1103 18-19 0.5820542250734988 1103 20-21 0.6036872577735579 1103 22-23 0.44137557213515777 1103 24-25 0.5322537914175669 1103 26-27 0.6916594059413583 1103 28-29 0.6360387065572013 1103 30-31 0.7356565907587438 1103 32-33 0.7564689060195988 1103 34-35 0.7500224272186102 1103 36-37 0.7071160926430693 1103 38-39 0.814487173232429 1103 40-41 0.6966848832589747 1103 42-43 0.8636758747822384 1103 44-45 0.7527527906617806 1103 46-47 0.7016365546282799 1103 48-49 0.8617507246819329 1103 50-51 0.8058121589715341 1103 52-53 0.7513478062882122 1103 54-55 0.7000695854219536 1103 56-57 0.6777698399288354 1103 58-59 0.6696503672905827 1103 60-61 0.7249821122808413 1103 62-63 0.7975468024702614 1103 64-65 0.792982512477824 1103 66-67 0.81082997519405 1103 68-69 0.6719288046342413 1103 70-71 0.6410431584180039 1103 72-73 0.7070557104197093 1103 74-75 0.6890085610140808 1103 76-77 0.7245449196730611 1103 78-79 0.697243404523217 1103 80-81 0.7662866473941712 1103 82-83 0.8070372926624891 1103 84-85 0.8144590737779822 1103 86-87 0.8418620845264186 1103 88-89 0.9220126008535843 1103 90-91 0.8701388240284764 1103 92-93 0.9116043953676218 1103 94-95 1.0106714986772758 1103 96-97 0.988941826514651 1103 98-99 1.018724774154741 1103 100-101 1.2532985901171187 1104 1 0.32811341859201093 1104 2 0.2743637549906168 1104 3 0.3504184764591294 1104 4 0.4264919865406398 1104 5 0.33363915615898065 1104 6 0.3275577053178509 1104 7 0.313191846861514 1104 8 0.3805760844295989 1104 9 0.3797438172178147 1104 10-11 0.5368655494306225 1104 12-13 0.4652123759212259 1104 14-15 0.4469687709408987 1104 16-17 0.5961538130620383 1104 18-19 0.5194876046086208 1104 20-21 0.5434223205026214 1104 22-23 0.44447166974364194 1104 24-25 0.5549768628238354 1104 26-27 0.5715481582033703 1104 28-29 0.5308054809035099 1104 30-31 0.6274363397338121 1104 32-33 0.6752711850137274 1104 34-35 0.6851651199433917 1104 36-37 0.7568197889309474 1104 38-39 0.6960562917869453 1104 40-41 0.7189734489136299 1104 42-43 0.8026390253488884 1104 44-45 0.8563515885829744 1104 46-47 0.7389637999560676 1104 48-49 0.8112022174261426 1104 50-51 0.6580236081343998 1104 52-53 0.7108832375277423 1104 54-55 0.8973169825694676 1104 56-57 0.9461594658301671 1104 58-59 0.8897713886482777 1104 60-61 0.8281000413196082 1104 62-63 0.8745048444312715 1104 64-65 0.8549290897536572 1104 66-67 0.7378199321417185 1104 68-69 0.6796107763138366 1104 70-71 0.7393377509400061 1104 72-73 0.7366820906675215 1104 74-75 0.7769887155561506 1104 76-77 0.8420453966783477 1104 78-79 0.8567048925130827 1104 80-81 0.8435161174644357 1104 82-83 0.7311115521324787 1104 84-85 0.8206864659138589 1104 86-87 0.8559360968962046 1104 88-89 0.9280267310791253 1104 90-91 0.8933847119117289 1104 92-93 0.9683174582816285 1104 94-95 0.9996964709973781 1104 96-97 0.9801320674322547 1104 98-99 1.1368337035663671 1104 100-101 1.3165263097669069 1105 1 0.05773470322766627 1105 2 0.08422232224456394 1105 3 0.24112314662572487 1105 4 0.27174781607360643 1105 5 0.3273359537178706 1105 6 0.26404832738251116 1105 7 0.28301732555205916 1105 8 0.3276063179784927 1105 9 0.2470109757423984 1105 10-11 0.19585937496296424 1105 12-13 0.2040451927424769 1105 14-15 0.09341447408859693 1105 16-17 0.24950429502683136 1105 18-19 0.2240796196746473 1105 20-21 0.36059865270888025 1105 22-23 0.3881230500039905 1105 24-25 0.45404809314252503 1105 26-27 0.65660045267105 1105 28-29 0.6251052787857674 1105 30-31 0.5732276976646773 1105 32-33 0.593306910860143 1105 34-35 0.5543761322226217 1105 36-37 0.6085579733549764 1105 38-39 0.7015618767238294 1105 40-41 0.6958013958348275 1105 42-43 0.62964502364445 1105 44-45 0.6169888066931364 1105 46-47 0.608239427987229 1105 48-49 0.715581170225434 1105 50-51 0.7529931580130409 1105 52-53 0.5591566350943431 1105 54-55 0.6452453047008042 1105 56-57 0.5892250738747435 1105 58-59 0.7674223523212262 1105 60-61 0.6923734332566518 1105 62-63 0.6703182818253559 1105 64-65 0.7277320093094026 1105 66-67 0.7716805316666182 1105 68-69 0.7199083631038476 1105 70-71 0.6320813978580127 1105 72-73 0.7587698740433986 1105 74-75 0.6652828166554237 1105 76-77 0.5874400031299238 1105 78-79 0.5935618995511938 1105 80-81 0.5398148808323633 1105 82-83 0.4744541013857422 1105 84-85 0.6133821208377626 1105 86-87 0.7134906096586633 1105 88-89 0.7193268347789221 1105 90-91 0.7352938097599271 1105 92-93 0.7869119013435188 1105 94-95 0.9198014324771222 1105 96-97 0.9519977232949657 1105 98-99 1.0613152335614018 1105 100-101 1.3183335225240214 1106 1 0.05257181430405922 1106 2 0.14543437179744245 1106 3 0.1621365849091383 1106 4 0.33905692662074216 1106 5 0.2078847739444072 1106 6 0.1655548170866794 1106 7 0.16220412196119582 1106 8 0.0817932903366696 1106 9 0.010242216408308025 1106 10-11 0.1370006413924969 1106 12-13 0.13022532983259083 1106 14-15 0.1359605269887325 1106 16-17 0.150965624478971 1106 18-19 -0.02887812112098942 1106 20-21 0.11033910676999881 1106 22-23 0.19918881318410087 1106 24-25 0.20336908939879805 1106 26-27 0.10770129774252268 1106 28-29 0.081674849737567 1106 30-31 0.16116882183354875 1106 32-33 0.26872706073118735 1106 34-35 0.22557736677487839 1106 36-37 0.263877259855839 1106 38-39 0.48231672623110455 1106 40-41 0.23927964548446568 1106 42-43 0.21827762282457996 1106 44-45 0.3291620634722179 1106 46-47 0.4047900715149595 1106 48-49 0.3272697413738328 1106 50-51 0.4586448052009757 1106 52-53 0.5078656008471576 1106 54-55 0.2718103103616727 1106 56-57 0.30977948224259677 1106 58-59 0.6406156020717333 1106 60-61 0.4946059027506102 1106 62-63 0.40678232089261 1106 64-65 0.5298654406111574 1106 66-67 0.6265393525078053 1106 68-69 0.6878767757948836 1106 70-71 0.5181413734878966 1106 72-73 0.6285372271928154 1106 74-75 0.7005478686260744 1106 76-77 0.6122006068743993 1106 78-79 0.6560353769938239 1106 80-81 0.6183283601705512 1106 82-83 0.5314636950654652 1106 84-85 0.5275885883488911 1106 86-87 0.6399863570878139 1106 88-89 0.6331722267206423 1106 90-91 0.6183027587623897 1106 92-93 0.670997217387864 1106 94-95 0.6896207867770094 1106 96-97 0.7728783458636457 1106 98-99 0.7249943137843111 1106 100-101 0.8999977892458624 1107 1 0.07917566485495797 1107 2 0.10375206743960419 1107 3 0.22983340274386066 1107 4 0.3771080686695356 1107 5 0.19080201454781331 1107 6 0.17798448428811753 1107 7 0.3154696588611401 1107 8 0.20619821638769054 1107 9 0.10319423257416815 1107 10-11 0.1865357255981337 1107 12-13 0.1701394389098212 1107 14-15 0.3063166043605037 1107 16-17 0.2574738290039136 1107 18-19 0.30317732283628374 1107 20-21 0.24133643953152983 1107 22-23 0.2170007803489753 1107 24-25 0.44589317633609227 1107 26-27 0.18523298054614656 1107 28-29 0.1828936762584732 1107 30-31 0.25998282675665507 1107 32-33 0.46253639401820124 1107 34-35 0.2876476297462389 1107 36-37 0.34717862145197387 1107 38-39 0.4865969019978209 1107 40-41 0.24454595174914573 1107 42-43 0.3336964285008506 1107 44-45 0.5332775206403042 1107 46-47 0.4264134127160304 1107 48-49 0.5235780376059438 1107 50-51 0.34550267955938807 1107 52-53 0.5436336898339427 1107 54-55 0.42610467474192504 1107 56-57 0.41710000157300087 1107 58-59 0.6287679142037419 1107 60-61 0.6052648208590519 1107 62-63 0.459255863051105 1107 64-65 0.5563029758554983 1107 66-67 0.6454299718027094 1107 68-69 0.5294757522452826 1107 70-71 0.40882573769099295 1107 72-73 0.4525789136937064 1107 74-75 0.5559425350234015 1107 76-77 0.5557828648264795 1107 78-79 0.41088396927951365 1107 80-81 0.34600098811022306 1107 82-83 0.39165392878070904 1107 84-85 0.4665630417122486 1107 86-87 0.6673191163118481 1107 88-89 0.5486239788775258 1107 90-91 0.49679722423680417 1107 92-93 0.5737648828099111 1107 94-95 0.502431160685056 1107 96-97 0.5859616878055505 1107 98-99 0.6941717722256158 1107 100-101 0.8947744284425596 1108 1 0.18683542383982754 1108 2 0.14685311537827772 1108 3 0.19624372607182394 1108 4 0.48177858525417605 1108 5 0.2126876228002459 1108 6 0.3986971753815425 1108 7 0.38445855341682034 1108 8 0.3085613020704372 1108 9 0.3624189694591209 1108 10-11 0.3861062213959272 1108 12-13 0.2251847961736715 1108 14-15 0.41620291108451823 1108 16-17 0.36687784530596446 1108 18-19 0.35209843257135987 1108 20-21 0.39763710835058674 1108 22-23 0.3469618454795338 1108 24-25 0.4779109919526334 1108 26-27 0.44547503493187435 1108 28-29 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32-33 -0.029978806479661557 2312 34-35 0.006110511797267293 2312 36-37 0.10355376349799172 2312 38-39 0.05576487736307456 2312 40-41 0.06970052921204228 2312 42-43 0.139743118887246 2312 44-45 0.18743126355667528 2312 46-47 0.08024900232841503 2312 48-49 0.06829652572102418 2312 50-51 -0.2132737308179813 2312 52-53 -0.08431724896465553 2312 54-55 -0.15131060290653764 2312 56-57 -0.07813238215325313 2312 58-59 -0.13340644311138306 2312 60-61 -0.07660923936559882 2312 62-63 -0.1516371564892367 2312 64-65 -0.012339027046227358 2312 66-67 -0.05885261110588047 2312 68-69 -0.08685093812658096 2312 70-71 -0.06317834889927099 2312 72-73 0.014502882240705617 2312 74-75 -0.007434497253601791 2312 76-77 -0.003686955461965624 2312 78-79 0.0020475096038445884 2312 80-81 -0.06963990080742732 2312 82-83 -0.1619450977267185 2312 84-85 -0.10832059413552031 2312 86-87 -0.06058595286728519 2312 88-89 0.006588428845773819 2312 90-91 0.12386462588666447 2312 92-93 0.009835683568450548 2312 94-95 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50-51 -0.40743180680467717 2313 52-53 -0.46109581008859735 2313 54-55 -0.31103589009866184 2313 56-57 -0.3562564889514235 2313 58-59 -0.4064596389671564 2313 60-61 -0.5145624386214571 2313 62-63 -0.5650877028585697 2313 64-65 -0.49681866060837976 2313 66-67 -0.5423844900867572 2313 68-69 -0.5767596000157269 2313 70-71 -0.4580567489012317 2313 72-73 -0.4293368561607096 2313 74-75 -0.4881966688932238 2313 76-77 -0.5908051515443233 2313 78-79 -0.48128492267505507 2313 80-81 -0.6527858268945259 2313 82-83 -0.5685619282630974 2313 84-85 -0.5636268424396818 2313 86-87 -0.6564230581081922 2313 88-89 -0.6887961967034038 2313 90-91 -0.5628996386603156 2313 92-93 -0.5989318963298196 2313 94-95 -0.5101253671483832 2313 96-97 -0.4686247505684804 2313 98-99 -0.4945034179173575 2313 100-101 -0.6269414972154266 2314 1 -0.6589221702734989 2314 2 -0.8906836657975994 2314 3 -0.5792557113948398 2314 4 -0.7905788351282013 2314 5 -0.7315408867953579 2314 6 -0.5689921833696445 2314 7 -0.445827028518309 2314 8 -0.5089435349601601 2314 9 -0.4660620871785568 2314 10-11 -0.6097736426679532 2314 12-13 -0.5658744363139832 2314 14-15 -0.7280242822886791 2314 16-17 -0.7562481700516628 2314 18-19 -2.45357447988561 2314 20-21 -1.1028326391100407 2314 22-23 -0.8803647844030777 2314 24-25 -0.9542158628566071 2314 26-27 -1.0435644222073748 2314 28-29 -0.7202877341719471 2314 30-31 -0.8019891011588598 2314 32-33 -0.8425850352934958 2314 34-35 -0.8967818571519999 2314 36-37 -0.9152243648668659 2314 38-39 -0.6653670877754578 2314 40-41 -0.6176044393359845 2314 42-43 -1.0298474177550574 2314 44-45 -1.0231496854316404 2314 46-47 -0.8116390854403193 2314 48-49 -0.8723585388088679 2314 50-51 -0.8851625984982334 2314 52-53 -0.9204588013330302 2314 54-55 -0.8062521681727013 2314 56-57 -0.724518412571765 2314 58-59 -0.7694458770850758 2314 60-61 -0.7726719886820206 2314 62-63 -0.8991565743730341 2314 64-65 -0.8048882263542971 2314 66-67 -0.8279407887632537 2314 68-69 -0.854882020207512 2314 70-71 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-1.5093399863365562 2315 28-29 -1.1806481600820575 2315 30-31 -1.4069550055803361 2315 32-33 -1.7172442355438449 2315 34-35 -1.6842727811562739 2315 36-37 -1.4827460424389827 2315 38-39 -1.630877048619837 2315 40-41 -2.630681200839973 2315 42-43 -2.1591864012415805 2315 44-45 -1.867278077014241 2315 46-47 -1.5806668238307964 2315 48-49 -1.8940591311733783 2315 50-51 -1.7425620058728484 2315 52-53 -1.624771396998316 2315 54-55 -1.5296122971566568 2315 56-57 -1.6689232938706233 2315 58-59 -1.774859043156809 2315 60-61 -1.4989696511763455 2315 62-63 -1.3510489779668369 2315 64-65 -1.3191599444355582 2315 66-67 -1.4091809236303554 2315 68-69 -1.6164113694595201 2315 70-71 -1.392229898566928 2315 72-73 -1.299980452279705 2315 74-75 -1.3663942866147565 2315 76-77 -1.3160845076066572 2315 78-79 -1.423566929122174 2315 80-81 -1.4457815902731497 2315 82-83 -1.3895253754517505 2315 84-85 -1.473334730347922 2315 86-87 -1.5583060118545546 2315 88-89 -1.5448982304078562 2315 90-91 -1.5161436506736514 2315 92-93 -1.567899965296732 2315 94-95 -1.6095175818066956 2315 96-97 -1.8009578258037706 2315 98-99 -1.7467755636600693 2315 100-101 -1.9131460743544864 2316 1 -0.7820208767851646 2316 2 -0.9542752301035158 2316 3 -1.3768197470676569 2316 4 -1.1853723676649963 2316 5 -0.7061063862404708 2316 6 -0.7028285466534925 2316 7 -0.9673670379651043 2316 8 -1.0735435474059614 2316 9 -1.3264807876749671 2316 10-11 -1.3326653176108394 2316 12-13 -1.1716819704183976 2316 14-15 -1.1766479746799234 2316 16-17 -1.9590609818082285 2316 18-19 -1.355048115951952 2316 20-21 -1.6862928060895896 2316 22-23 -1.29754112848795 2316 24-25 -1.132213622930088 2316 26-27 -1.2989183767616126 2316 28-29 -1.6607113929555766 2316 30-31 -1.556287615158432 2316 32-33 -1.6130824839175943 2316 34-35 -2.031645046125327 2316 36-37 -1.6687550989219062 2316 38-39 -1.5756996014962965 2316 40-41 -2.0576198085097346 2316 42-43 -2.2111064954149597 2316 44-45 -2.4331622678274023 2316 46-47 -2.3498212954461657 2316 48-49 -2.94401103655332 2316 50-51 -2.501407026685925 2316 52-53 -3.2936088465546263 2316 54-55 -4.36028651730145 2316 56-57 -3.0622362800371654 2316 58-59 -2.6136722010178346 2316 60-61 -2.3101755400287978 2316 62-63 -2.865423332266495 2316 64-65 -2.883526603652804 2316 66-67 -2.604983523849679 2316 68-69 -2.5774653773506806 2316 70-71 -2.8192207913325262 2316 72-73 -5.9595649592941555 2316 74-75 -3.6235749032772766 2316 76-77 -2.946263092875345 2316 78-79 -2.9171410312037516 2316 80-81 -2.8285116583979892 2316 82-83 -2.8235200428263845 2316 84-85 -2.9361401753479086 2316 86-87 -2.745716570189227 2316 88-89 -2.6142115680574776 2316 90-91 -2.66547258240281 2316 92-93 -2.9544197514566513 2316 94-95 -3.2166422312643803 2316 96-97 -3.187445647338297 2316 98-99 -3.456494150278907 2316 100-101 -3.48606011132366 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97909.0 3 17578.0 4 15292.0 5 14979.0 6 17167.0 7 21308.0 8 26661.0 9 31456.0 10 36270.0 11 43033.0 12 44057.0 13 49262.0 14 55813.0 15 65481.0 16 81654.0 17 106810.0 18 150764.0 19 224656.0 20 324838.0 21 247570.0 22 141807.0 23 131461.0 24 141565.0 25 150658.0 26 162136.0 27 182209.0 28 211089.0 29 245595.0 30 290575.0 31 346867.0 32 422037.0 33 524128.0 34 678777.0 35 968307.0 36 1395505.0 37 1328859.0 38 884392.0 39 122029.0 40 87.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.891486024245854 49.91037904454162 21.575362930586724 17.6227720006258 2 16.38355323725074 51.34644967402967 16.65822839029812 15.611768698421475 3 47.746140079103036 18.79825964522336 17.30803683041009 16.147563445263515 4 16.270135660520396 17.392710412261273 18.331346846254316 48.00580708096402 5 16.514486015538157 49.05594425188643 19.15527830225875 15.274291430316659 6 48.04061134003149 18.357926718987226 17.26589491988921 16.33556702109207 7 17.250783661092864 17.921763912843332 49.58907902192529 15.238373404138509 8 47.520095855205156 18.652622662105696 18.091218774073017 15.73606270861613 9 47.01881762262578 18.372668687850233 18.701665600032825 15.906848089491163 10-11 15.901636990098982 33.967881047484326 33.79203374282812 16.33844821958857 12-13 15.76682025828308 18.7650163343796 33.622489835260296 31.845673572077025 14-15 16.06285017356225 49.702908965819184 17.87989617537605 16.354344685242516 16-17 31.72460018192231 18.477892326827966 17.997961041248814 31.79954645000091 18-19 16.229895115696305 34.05647975005102 18.024850121507146 31.68877501274553 20-21 31.822491604239346 34.01858567837856 18.15968488146788 15.999237835914215 22-23 31.67212731330533 18.42212580023362 33.74920713791706 16.156539748543988 24-25 16.068218175954012 33.70214467065634 18.71929705390773 31.51034009948192 26-27 16.361202131699994 24.521872843994174 42.87856629905014 16.238358725255686 28-29 29.011317136726795 18.235532856190492 36.62121825083372 16.131931756248992 30-31 26.28825230491612 19.863070427353506 37.012826706918304 16.835850560812073 32-33 19.947558757319406 22.14323069425227 38.87930851664027 19.02990203178805 34-35 19.313327392295342 21.23676303637231 39.62451406002583 19.825395511306525 36-37 19.319234745459706 21.14869714129994 39.71880289021285 19.813265223027503 38-39 19.687567311344903 21.159710506567485 39.40327720523846 19.749444976849148 40-41 19.575161955267237 21.00106460368086 39.33167619381 20.092097247241913 42-43 20.095292827062387 21.244426041390565 39.25811571924981 19.40216541229724 44-45 19.49440555253394 21.362401113171966 39.1933565039069 19.94983683038719 46-47 19.808924002564165 21.211222388596024 39.15815979461061 19.821693814229203 48-49 19.72884974438147 21.286959126093887 39.27952952566186 19.70466160386278 50-51 19.858868355862064 21.08975621633783 38.87096485374091 20.1804105740592 52-53 20.211912694074744 21.9206577314192 37.80206076747203 20.065368807034027 54-55 20.817054985307283 22.605880517846664 36.361068135399776 20.215996361446276 56-57 21.14330684889012 23.426355253003553 35.09547439288346 20.33486350522287 58-59 21.15829577372918 24.363894439071004 34.107605414665635 20.370204372534182 60-61 21.13180839678176 24.71556002321779 33.63135070372683 20.52128087627361 62-63 20.900593793103 24.86455031213869 33.461776823175136 20.773079071583176 64-65 20.691530118634144 24.50199781125643 33.7674571507777 21.039014919331724 66-67 20.703877939656486 23.997532671297474 34.69312349168227 20.605465897363768 68-69 20.55053825055789 23.615148625259987 35.211162142822346 20.62315098135978 70-71 20.40892892704016 23.54141881196722 35.69853018234319 20.35112207864943 72-73 20.143220780998007 23.3325520672341 35.65253325282591 20.871693898941977 74-75 20.605488001271734 23.517919571827676 35.13882371560783 20.737768711292762 76-77 20.894625531084557 24.080675182360352 34.30742082045039 20.717278466104702 78-79 20.77638031155752 24.281918864593504 34.07538014324133 20.86632068060765 80-81 20.684505938023314 24.917004126447143 33.49187302945605 20.9066169060735 82-83 21.538909091782145 24.972443362525503 32.81881105082561 20.66983649486674 84-85 21.378317825372072 25.450833300320642 32.2480261412487 20.922822733058588 86-87 21.15248671824103 25.75561763580689 32.109362619631874 20.982533026320212 88-89 20.9956369358415 25.372205935827996 32.104869595750074 21.52728753258043 90-91 21.00525490387918 25.308952003890724 32.212615300690274 21.473177791539825 92-93 21.149406614132552 25.611403444319475 32.21802836065885 21.02116158088913 94-95 21.135478342396475 25.419140698543824 31.7140041491908 21.731376809868905 96-97 21.044871276797494 25.180213683796953 32.10178147182877 21.67313356757678 98-99 21.438414124140603 25.555591066569296 31.49137705059224 21.51461775869786 100-101 21.75403612574337 25.200808595412038 31.33645786720208 21.708697411642515 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 27104.0 1 15844.5 2 3304.0 3 1780.0 4 1453.5 5 1271.5 6 1484.0 7 1935.5 8 1752.0 9 1813.5 10 2877.0 11 17765.5 12 323918.5 13 354317.0 14 79774.0 15 66821.5 16 68659.0 17 71737.5 18 73601.5 19 73390.0 20 72419.5 21 71345.0 22 70431.5 23 70543.0 24 71699.0 25 74064.0 26 77379.0 27 82252.5 28 86180.0 29 89841.0 30 96077.0 31 103853.5 32 111289.5 33 119693.5 34 134995.0 35 146794.5 36 155377.0 37 167006.0 38 177801.0 39 188541.0 40 202403.0 41 218839.0 42 232584.5 43 245575.5 44 259194.5 45 271595.5 46 280116.0 47 290740.5 48 303470.5 49 314711.5 50 322621.5 51 325492.5 52 331414.5 53 339231.5 54 330205.5 55 310380.5 56 296191.0 57 280324.5 58 279387.0 59 248932.5 60 191748.0 61 157184.0 62 126194.5 63 99056.0 64 78257.0 65 63424.0 66 51020.0 67 41342.5 68 32231.5 69 24532.0 70 19534.0 71 16050.0 72 13386.5 73 10731.5 74 8540.5 75 6601.5 76 5108.0 77 4083.0 78 3343.0 79 2867.0 80 2591.5 81 2355.0 82 2118.0 83 1906.0 84 1696.5 85 1462.0 86 1247.5 87 1011.5 88 743.0 89 579.5 90 437.5 91 334.5 92 265.5 93 199.5 94 138.0 95 97.5 96 69.0 97 48.0 98 31.0 99 20.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10231344170838648 2 0.08473456851415824 3 0.09114415765949402 4 0.19032779998802077 5 0.0769250691030705 6 0.09539388525195536 7 0.10033356861825157 8 0.08903429290182499 9 0.0814547787486822 10-11 0.07879994892327402 12-13 0.07148041810519945 14-15 0.07827998225313758 16-17 0.06814063218547692 18-19 0.059611178923431006 20-21 0.05417652728460106 22-23 0.04419216728207722 24-25 0.046537016977211756 26-27 0.04581206344673306 28-29 0.04472713299077529 30-31 0.035307736774072784 32-33 0.0392874816724248 34-35 0.05193167117987737 36-37 0.0469869881340606 38-39 0.051116723418028906 40-41 0.049021857698921506 42-43 0.047151977558238516 44-45 0.050601756427413 46-47 0.04408717401214582 48-49 0.040062431998108924 50-51 0.044417152860501644 52-53 0.039767450906396905 54-55 0.04361220445769426 56-57 0.0426922634259144 58-59 0.049726812511318025 60-61 0.04858688558063428 62-63 0.05324158721425956 64-65 0.04951682597145523 66-67 0.04404717657598148 68-69 0.044597141323241184 70-71 0.05285161221165723 72-73 0.04046740603927289 74-75 0.048521889746867226 76-77 0.04704198460878658 78-79 0.04573206857440438 80-81 0.045112108313857084 82-83 0.04410217305070745 84-85 0.04484712529926831 86-87 0.04976680994748237 88-89 0.04225729130762718 90-91 0.04549208395741833 92-93 0.0415223384181074 94-95 0.04474713170885747 96-97 0.030648035460926957 98-99 0.03742260121126236 100-101 0.02562835722230205 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0000641E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.172949303156486 #Duplication Level Percentage of deduplicated Percentage of total 1 90.02703914707871 44.26895031892587 2 5.507126648948531 5.416033190296163 3 1.7276876717056207 2.5486649487740687 4 0.8225816573917959 1.6179506454653287 5 0.4860227392740742 1.1949585759252646 6 0.3039088812281649 0.8966457605646935 7 0.21603097026276263 0.7436015964049774 8 0.15891688522881237 0.6251529552617543 9 0.1183135348377459 0.5236042905408346 >10 0.6120965324704464 4.923878525003609 >50 0.007481769169503478 0.24908121640399034 >100 0.006960567127938102 0.8639169633725706 >500 0.0024538807823597667 0.842634215954262 >1k 0.0028561562980698577 2.743832841880388 >5k 3.620479814454749E-4 1.3236505991956322 >10k+ 1.609102139757666E-4 31.21744335603063 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1512225 15.12128072590547 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 31656 0.3165397098046015 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23450 0.23448496951345418 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCCGCAGCGGCTTCTGA 10816 0.1081530673883804 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.999359041085466E-6 0.0 0.0 9.999359041085466E-6 0.0 2 2.99980771232564E-5 9.999359041085466E-6 0.0 9.999359041085466E-6 0.0 3 4.999679520542733E-5 9.999359041085466E-6 0.0 9.999359041085466E-6 0.0 4 5.99961542465128E-5 9.999359041085466E-6 0.0 9.999359041085466E-6 0.0 5 6.999551328759827E-5 9.999359041085466E-6 0.0 9.999359041085466E-6 9.999359041085466E-6 6 7.999487232868373E-5 9.999359041085466E-6 0.0 9.999359041085466E-6 9.999359041085466E-6 7 7.999487232868373E-5 9.999359041085466E-6 0.0 9.999359041085466E-6 9.999359041085466E-6 8 9.999359041085466E-5 9.999359041085466E-6 0.0 9.999359041085466E-6 9.999359041085466E-6 9 1.199923084930256E-4 9.999359041085466E-6 0.0 9.999359041085466E-6 9.999359041085466E-6 10-11 1.2499198801356834E-4 9.999359041085466E-6 0.0 9.999359041085466E-6 9.999359041085466E-6 12-13 1.5998974465736746E-4 9.999359041085466E-6 0.0 9.999359041085466E-6 9.999359041085466E-6 14-15 1.9498750130116657E-4 9.999359041085466E-6 0.0 9.999359041085466E-6 1.4999038561628198E-5 16-17 2.24985578424423E-4 9.999359041085466E-6 0.0 9.999359041085466E-6 1.9998718082170932E-5 18-19 2.2998525794496572E-4 9.999359041085466E-6 0.0 1.4999038561628198E-5 1.9998718082170932E-5 20-21 2.6998269410930757E-4 9.999359041085466E-6 0.0 1.9998718082170932E-5 3.9997436164341864E-5 22-23 2.7998205315039307E-4 9.999359041085466E-6 0.0 1.9998718082170932E-5 3.9997436164341864E-5 24-25 3.1997948931473496E-4 9.999359041085466E-6 0.0 1.9998718082170932E-5 3.9997436164341864E-5 26-27 3.899750026023332E-4 9.999359041085466E-6 0.0 1.9998718082170932E-5 3.9997436164341864E-5 28-29 4.0497404116396135E-4 9.999359041085466E-6 0.0 1.9998718082170932E-5 3.9997436164341864E-5 30-31 4.2497275924613235E-4 9.999359041085466E-6 0.0 2.4998397602713666E-5 3.9997436164341864E-5 32-33 4.49971156848846E-4 1.4999038561628198E-5 0.0 2.99980771232564E-5 4.999679520542733E-5 34-35 4.6996987493101695E-4 1.9998718082170932E-5 0.0 3.499775664379913E-5 5.499647472597007E-5 36-37 5.199666701364442E-4 1.9998718082170932E-5 0.0 3.9997436164341864E-5 5.99961542465128E-5 38-39 5.999615424651279E-4 2.99980771232564E-5 0.0 4.999679520542733E-5 5.99961542465128E-5 40-41 7.199538509581536E-4 2.99980771232564E-5 0.0 4.999679520542733E-5 5.99961542465128E-5 42-43 8.0494840280738E-4 2.99980771232564E-5 0.0 4.999679520542733E-5 5.99961542465128E-5 44-45 8.99942313697692E-4 2.99980771232564E-5 0.0 5.99961542465128E-5 6.999551328759827E-5 46-47 9.799371860263757E-4 2.99980771232564E-5 0.0 5.99961542465128E-5 6.999551328759827E-5 48-49 0.0010699314173961449 2.99980771232564E-5 0.0 6.499583376705553E-5 6.999551328759827E-5 50-51 0.0011349272511632005 3.9997436164341864E-5 0.0 6.999551328759827E-5 7.999487232868373E-5 52-53 0.0011849240463686279 3.9997436164341864E-5 0.0 6.999551328759827E-5 7.999487232868373E-5 54-55 0.0012749182777383968 4.4997115684884594E-5 0.0 6.999551328759827E-5 7.999487232868373E-5 56-57 0.0014149093043135934 4.999679520542733E-5 0.0 7.999487232868373E-5 8.499455184922646E-5 58-59 0.0015299019332860764 4.999679520542733E-5 0.0 7.999487232868373E-5 8.99942313697692E-5 60-61 0.0016598936008201874 4.999679520542733E-5 0.0 8.499455184922646E-5 9.499391089031194E-5 62-63 0.0017948849478748412 4.999679520542733E-5 0.0 8.99942313697692E-5 1.0999294945194014E-4 64-65 0.001964874051573294 4.999679520542733E-5 0.0 8.99942313697692E-5 1.0999294945194014E-4 66-67 0.0021448625143128328 4.999679520542733E-5 0.0 9.999359041085466E-5 1.0999294945194014E-4 68-69 0.0022498557842442297 4.999679520542733E-5 0.0 9.999359041085466E-5 1.1499262897248286E-4 70-71 0.0024098455289015973 4.999679520542733E-5 0.0 1.0999294945194014E-4 1.199923084930256E-4 72-73 0.0025798346326000505 4.999679520542733E-5 0.0 1.199923084930256E-4 1.199923084930256E-4 74-75 0.002794820851983388 5.499647472597007E-5 0.0 1.199923084930256E-4 1.2499198801356834E-4 76-77 0.00305480418705161 5.99961542465128E-5 0.0 1.2499198801356834E-4 1.3499134705465378E-4 78-79 0.003334786240202003 5.99961542465128E-5 0.0 1.2999166753411106E-4 1.4499070609573928E-4 80-81 0.0036447663704756526 5.99961542465128E-5 4.999679520542733E-6 1.2999166753411106E-4 1.5998974465736746E-4 82-83 0.004099737206845041 5.99961542465128E-5 9.999359041085466E-6 1.2999166753411106E-4 1.5998974465736746E-4 84-85 0.0046247035565020285 5.99961542465128E-5 9.999359041085466E-6 1.4499070609573928E-4 1.6998910369845293E-4 86-87 0.005474649074994294 6.999551328759827E-5 9.999359041085466E-6 1.5499006513682473E-4 1.799884627395384E-4 88-89 0.006624575364719122 6.999551328759827E-5 9.999359041085466E-6 1.5998974465736746E-4 1.9998718082170933E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 316705 0.0 91.99811 1 AGTGGTA 317425 0.0 91.323586 5 AGCAGTG 319830 0.0 90.99829 2 GGTATCA 318920 0.0 90.94297 8 GTATCAA 318900 0.0 90.90092 9 TGGTATC 318870 0.0 90.86959 7 GTGGTAT 319745 0.0 90.65576 6 GCAGTGG 320910 0.0 90.48583 3 CAGTGGT 321200 0.0 90.05011 4 ATCAACG 319465 0.0 45.925133 10-11 CAACGCA 319420 0.0 45.909344 12-13 TCAACGC 319105 0.0 45.84001 12-13 ACGCAGA 321050 0.0 45.73772 14-15 AACGCAG 320840 0.0 45.666958 14-15 TATCAAC 320395 0.0 45.5716 10-11 CGCAGAG 321640 0.0 45.541252 16-17 AGAGTAC 314690 0.0 45.45878 18-19 GAGTACT 183205 0.0 45.330887 20-21 CAGAGTA 319280 0.0 45.315525 18-19 GCAGAGT 322770 0.0 45.226543 16-17 >>END_MODULE